P08967 (PGKC_CRIFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase, glycosomal
Short name=Phosphoglycerate kinase C
EC=2.7.2.3

Gene names

Name:	PGKC
Synonyms:	PGK-C

Organism

Crithidia fasciculata

Taxonomic identifier

5656 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmaniinae › Crithidia

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Glycosome.
Domain	This glycosomal PGK has a C-terminal extension of 38 AA which is not present in the cytosolic isoenzyme. This domain most likely serves as topogenic signal to direct the glycosomal PKG to the glycosome.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Glycosome Peroxisome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	glycosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Phosphoglycerate kinase, glycosomal		PRO_0000145850
Regions
	Nucleotide binding	372 – 375	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	62 – 65	4	Substrate binding By similarity
	Region	417 – 455	39	Topogenic signal Potential
	Compositional bias	422 – 427	6	Poly-Ala
Sites
	Binding site	39	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	220	1	ATP By similarity
	Binding site	342	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08967 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: D8BF53AE5A0E1BFE	FASTA	455	47,843
	10 20 30 40 50 60 MSLVPKKSID DAVVKGKKVL IRVDFNVPVK NGEITNDFRI RSALPTIQKV LKEGGSCILM 70 80 90 100 110 120 SHLGRPKGAK MSDPKPAKSV RGYEEAATLR PVAARLSELL GQKVEFAPDC LDAASYAAKL 130 140 150 160 170 180 KGGDVLLLEN VRFYAEEGSK KEEERDAMAK VLAAYGDVYV SDAFGTAHRD SADMTGIPKV 190 200 210 220 230 240 LGAGYAGYLM EKEINYFAQV LNNPPRPLVA IVGGAKVSDK IQLLDNMLGR INYLVIGGAM 250 260 270 280 290 300 AYTFQKAQGH AIGISMCEED KLDLAKSLLK KAQERNVEVL LPVDHVCNKE FQGVDAPLVT 310 320 330 340 350 360 KDVEIPEGYM ALDIGPKTIK IYEDVIAKCK STIWNGPMGV FEMPCYSKGT FAVAKAMGNG 370 380 390 400 410 420 TQKNGLMSII GGGDTASAAE LSGEAKNMSH VSTGGGASLE LLEGKSLPGV TVLTNKDAKA 430 440 450 PAAAAAAGGD CPCGSGCAAV PAAATATVSM VLASP

References

[1]	"The topogenic signal of the glycosomal (microbody) phosphoglycerate kinase of Crithidia fasciculata resides in a carboxy-terminal extension." Swinkels B.W., Evers R., Borst P. EMBO J. 7:1159-1165(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07459 Genomic DNA. Translation: CAA30342.1.
PIR	TVCRGG. S00487.
3D structure databases
ProteinModelPortal	P08967.
SMR	P08967. Positions 6-416.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
InterPro	IPR027250. Pgk_euglenozoa. IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit. PIRSF500126. Pgk_euglenozoa. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKC_CRIFA

Accession

Primary (citable) accession number: P08967

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)