Reviewed, UniProtKB/Swiss-Prot P08966 (PGKB_CRIFA)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase, cytosolic
    EC=2.7.2.3
Alternative name(s):
    Phosphoglycerate kinase B

Gene names

Name:	PGKB
Synonyms:	PGK-B

Organism

Crithidia fasciculata

Taxonomic identifier

5656 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Crithidia

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Domain	This cytosolic PGK lacks a C-terminal extension of 38 AA which is present in the glycosomal isoenzyme.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Phosphoglycerate kinase, cytosolic		PRO_0000145849
Regions
	Nucleotide binding	372 – 375	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	62 – 65	4	Substrate binding By similarity
Sites
	Binding site	39	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	220	1	ATP By similarity
	Binding site	342	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08966-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 7D7C2D3625E05DB6	FASTA	417	44,603
	10 20 30 40 50 60 MSLAPKKTID DAVVKGKKVL IRVDFNVPVK NGEITNDFRI RSALPTIQKV LKEGGSCILM 70 80 90 100 110 120 SHLGRPKGAK MSDPKPAKSV RGYEEAATLR PVAARLSELL GQKVEFAPDC LDAASYAAKL 130 140 150 160 170 180 KGGDVLLLEN VRFYAEEGSK KEEERDAMAK VLAAYGDVYV SDAFGTAHRD SADMTGIPKV 190 200 210 220 230 240 LGAGYAGYLM EKEINYFAQV LNNPPRPLVA IVGGAKVSDK IQLLDNMLGR INYLVIGGAM 250 260 270 280 290 300 AYTFQKAQGH AIGISMCEED KLDLAKSLLK KAQERNVEVL LPVDHVCNKE FQGVDAPLVT 310 320 330 340 350 360 KDVEIPEGYM ALDIGPKTIK IYEDVIAKCK STIWNGPMGV FEMPCYSKGT FAVAKAMGNG 370 380 390 400 410 TQKNGLMSII GGGDTASAAE LSGEAKNMSH VSTGGGASLE LLEGKSLPGV TVLTNKE

References

[1]	"The topogenic signal of the glycosomal (microbody) phosphoglycerate kinase of Crithidia fasciculata resides in a carboxy-terminal extension." Swinkels B.W., Evers R., Borst P. EMBO J. 7:1159-1165(1988) [PubMed: 3402434] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07458 Genomic DNA. Translation: CAA30341.1.
PIR	TVCRGC. S00486.
3D structure databases
SMR	P08966. Positions 6-416.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.2.3. 571.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKB_CRIFA

Accession

Primary (citable) accession number: P08966

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	November 24, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)