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P08964 (MYO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin-1
Alternative name(s):
Type II myosin
Gene names
Name:MYO1
Ordered Locus Names:YHR023W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length1928 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for cell division.

Miscellaneous

Present with 2140 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Contains 1 IQ domain.

Contains 1 myosin head-like domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MLC1P531413EBI-11650,EBI-10988

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19281928Myosin-1
PRO_0000123484

Regions

Domain1 – 793793Myosin head-like
Domain794 – 82330IQ
Nucleotide binding180 – 1878ATP By similarity
Region460 – 52970Actin-binding By similarity
Coiled coil856 – 19111056 Potential

Amino acid modifications

Modified residue11711Phosphoserine Ref.7 Ref.8
Modified residue12061Phosphoserine Ref.8
Modified residue14761Phosphoserine Ref.6
Modified residue14771Phosphothreonine Ref.6

Experimental info

Sequence conflict361K → I in CAA37894. Ref.1
Sequence conflict461I → T in CAA37894. Ref.1
Sequence conflict461I → T in CAA29550. Ref.4
Sequence conflict591V → S in CAA37894. Ref.1
Sequence conflict591V → S in CAA29550. Ref.4
Sequence conflict861L → F in CAA37894. Ref.1
Sequence conflict3301Missing in CAA37894. Ref.1
Sequence conflict3301Missing in CAA29550. Ref.4
Sequence conflict3431N → S in CAA37894. Ref.1
Sequence conflict3431N → S in CAA29550. Ref.4
Sequence conflict421 – 4266QQAKFI → TKLSSL in CAA37894. Ref.1
Sequence conflict5151D → S in CAA37894. Ref.1
Sequence conflict529 – 5357SKGPPTG → ARGHDR in CAA37894. Ref.1
Sequence conflict529 – 5357SKGPPTG → ARGHDR in CAA29550. Ref.4
Sequence conflict5411D → V in CAA37894. Ref.1
Sequence conflict5411D → V in CAA29550. Ref.4
Sequence conflict550 – 5512TD → LM in CAA37894. Ref.1
Sequence conflict5731R → A in CAA37894. Ref.1
Sequence conflict5821H → D in CAA37894. Ref.1
Sequence conflict588 – 59912EYTVE…LSKNK → NTLWKAGYPKT in CAA37894. Ref.1
Sequence conflict5991Missing in CAA29550. Ref.4
Sequence conflict627 – 6326EKSSSA → GKNLLVC in CAA37894. Ref.1
Sequence conflict627 – 6326EKSSSA → GKNLLVC in CAA29550. Ref.4
Sequence conflict6951R → S in CAA37894. Ref.1
Sequence conflict6951R → S in CAA29550. Ref.4
Sequence conflict736 – 7427ENSTTTT → RKFNHHD in CAA29550. Ref.4
Sequence conflict7561E → R in CAA37894. Ref.1
Sequence conflict7561E → R in CAA29550. Ref.4
Sequence conflict773 – 78412NTKLF…AGVLA → ILTVFQKLEYWS in CAA37894. Ref.1
Sequence conflict793 – 7942KL → NV in CAA37894. Ref.1
Sequence conflict8961N → T in CAA37894. Ref.1
Sequence conflict9001N → NSQITKINTNITETPQSTYI GERPKRVICGN in CAA37894. Ref.1
Sequence conflict9061N → I in CAA37894. Ref.1
Sequence conflict9111N → K in CAA37894. Ref.1
Sequence conflict915 – 93016NESLL…SETLQ → RIAIKILKPAINIT in CAA37894. Ref.1
Sequence conflict934 – 9396DDLVSE → MTLFL in CAA37894. Ref.1
Sequence conflict951 – 9588AQNLEEAH → RKILKKLD in CAA37894. Ref.1
Sequence conflict10021S → C in CAA37894. Ref.1
Sequence conflict10491L → D in CAA37894. Ref.1
Sequence conflict10561C → S in CAA37894. Ref.1
Sequence conflict10601M → I in CAA37894. Ref.1
Sequence conflict10851A → E in CAA37894. Ref.1
Sequence conflict11231V → C in CAA37894. Ref.1
Sequence conflict11331L → S in CAA37894. Ref.1
Sequence conflict1144 – 11463KSN → NLI in CAA37894. Ref.1
Sequence conflict1159 – 116810RETKEQEQKK → TRKKEEQDKE in CAA37894. Ref.1
Sequence conflict1179 – 120426SKIKE…KRISG → ELKVKEWKARCHRKYLKSIL KQKNIR in CAA37894. Ref.1
Sequence conflict12241P → S in CAA37894. Ref.1
Sequence conflict12281E → Q in CAA37894. Ref.1
Sequence conflict12531E → Q in CAA37894. Ref.1
Sequence conflict1311 – 132313PDKES…KLMLE → LTKSLILTNGNAS in CAA37894. Ref.1
Sequence conflict14001D → H in CAA37894. Ref.1
Sequence conflict1454 – 1554101SEQLD…ETLLE → P in CAA37894. Ref.1
Sequence conflict15681D → V in CAA37894. Ref.1
Sequence conflict1630 – 164617DLLKQ…VEMLN → SEAARSLYKSGGNVD in CAA37894. Ref.1
Sequence conflict1699 – 17057Missing in CAA37894. Ref.1
Sequence conflict1725 – 173713TLQLQ…NSRNG → NTTANGTKFKEW in CAA37894. Ref.1
Sequence conflict1754 – 17574FDDE → LMM in CAA37894. Ref.1
Sequence conflict17771D → E in CAA37894. Ref.1
Sequence conflict17881R → T in CAA37894. Ref.1
Sequence conflict18251S → D in CAA37894. Ref.1
Sequence conflict18821S → W in CAA37894. Ref.1
Sequence conflict1902 – 19043FWK → NSGKRLDADDL in CAA37894. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P08964 [UniParc].

Last modified February 1, 1995. Version 3.
Checksum: 6F54C7611F43DC9F

FASTA1,928223,636
        10         20         30         40         50         60 
MTGGQSCSSN MIVWIPDEKE VFVKGELMST DINKNKFTGQ EEQIGIVHPL DSTEVSNLVQ 

        70         80         90        100        110        120 
VRISDVFPVN PSTFDKVENM SELTHLNEPS VLYNLEKRYD CDLIYTYSGL FLVAINPYHN 

       130        140        150        160        170        180 
LNLYSEDHIN LYHNKHNRLS KSRLDENSHE KLPPHIFAIA EEAYENLLSE GKDQSILVTG 

       190        200        210        220        230        240 
ESGAGKTENT KKILQYLASI TSGSPSNIAP VSGSSIVESF EMKILQSNPI LESFGNAQTV 

       250        260        270        280        290        300 
RNNNSSRFGK FIKIEFNEHG MINGAHIEWY LLEKSRIVHQ NSKERNYHIF YQLLSGLDDS 

       310        320        330        340        350        360 
ELKNLRLKSR NVKDYKILSN SNQDIIPGIN DVENFKELLS ALNIIGFSKD QIRWIFQVVA 

       370        380        390        400        410        420 
IILLIGNIEF VSDRAEQASF KNDVSAICSN LGVDEKDFQT AILRPRSKAG KEWVSQSKNS 

       430        440        450        460        470        480 
QQAKFILNAL SRNLYERLFG YIVDMINKNL DHGSATLNYI GLLDIAGFEI FENNSFEQLC 

       490        500        510        520        530        540 
INYTNEKLQQ FFNNHMFVLE QSEYLKENIQ WDYIDYGKDL QLTIDLIESK GPPTGVLPLL 

       550        560        570        580        590        600 
DEEAVLPKST DESFYSKLIS TWDQNSSKFK RSRLKNGFIL KHYAGDVEYT VEGWLSKNKD 

       610        620        630        640        650        660 
PLNDNLLSLL SSSQNDIISK LFQPEGEKSS SAGVEANISN QEVKKSARTS TFKTTSSRHR 

       670        680        690        700        710        720 
EQQITLLNQL ASTHPHFVRC IIPNNVKKVK TFNRRLILDQ LRCNGVLEGI RLAREGYPNR 

       730        740        750        760        770        780 
IAFQEFFQRY RILYPENSTT TTFSSKLKAS TKQNCEFLLT SLQLDTKVYK IGNTKLFFKA 

       790        800        810        820        830        840 
GVLADLEKQK DVKLNNIMIK LTATIRGYTV RKEITYHLQK LKKTRVIGNT FRLYNRLVKE 

       850        860        870        880        890        900 
DPWFNLFIRI KPLLTSSNDM TRTKKFNEQI NKLKNDLQEM ESKKKFLEEK NQKTVNELEN 

       910        920        930        940        950        960 
TQDLLNQEKE NLRKNESLLN RVKTSSETLQ KQFDDLVSEK DEISREKLEV AQNLEEAHQK 

       970        980        990       1000       1010       1020 
IQGLQETIRE REATLEKLHS KNNELIKQIS DLNCDISKEQ SSQSLIKESK LKLENEIKRL 

      1030       1040       1050       1060       1070       1080 
KDVINSKEEE IKSFNDKLSS SEEDLDIKLV TLEKNCNIAM SRLQSLVTEN SDLRSKNENF 

      1090       1100       1110       1120       1130       1140 
KKEKAALNNQ LKNKESELLK MKEKIDNHKK ELATFSKQRD DAVSEHGKIT AELKETRIQL 

      1150       1160       1170       1180       1190       1200 
TEYKSNYQKI KEEYSNFQRE TKEQEQKKRN SLVESLNDSK IKELEARLSQ EISLNQYLNK 

      1210       1220       1230       1240       1250       1260 
RISGNSVETN ISSTRRSTSY SDDPLDKEDI IKKYYDLQLA FTEITRNLEN EIEEKKNLIS 

      1270       1280       1290       1300       1310       1320 
RLRFTETRLA SSSFEDQKIK AQMKKLKKLI QDMDPSIPLD SILNEPLDNC PDKESDINKL 

      1330       1340       1350       1360       1370       1380 
MLEVDYLKRQ LDIETRAHYD AENAISALHS KFRKIQGESS LSSSDIYKLK FEASEERVKS 

      1390       1400       1410       1420       1430       1440 
LEDKLKTMPL RDRTNLPVGD IIKNRDSISK YEEEIRYYKL ENYKLQEILN ESNGKLSQLT 

      1450       1460       1470       1480       1490       1500 
LDLRQSKSKE ALLSEQLDRL QKDLESTERQ KELLSSTIKQ QKQQFENCMD DLQGNELRLR 

      1510       1520       1530       1540       1550       1560 
EHIHALKQAE EDVKNMASII EKLKTQNKQK EKLIWEREME RNDSDMQLQE TLLELKRVQD 

      1570       1580       1590       1600       1610       1620 
VKKILSDDLA HLKERLSAVE DRSQYTDEIN RLKEELNCSL KAETNLKKEF ATLKYKLETS 

      1630       1640       1650       1660       1670       1680 
TNDSEAKISD LLKQLDHYTK VVEMLNNEKD AISLAEKELY QKYEALNTEC ESLKGKIVSL 

      1690       1700       1710       1720       1730       1740 
TKIKQELESD LNQKTDALQI SNAALSSSTQ KNKEITEKIK YLEETLQLQM EQNSRNGELV 

      1750       1760       1770       1780       1790       1800 
KTLQASCNGY KDKFDDEKQK NIDLYEENQT LQKLNTDLQL QLKNLHERLS DTTEKNAWLS 

      1810       1820       1830       1840       1850       1860 
KIHELENMVS LETDLKYEEM KKNKSLERAV EELQTKNSQQ TDVIELANKN RSEFEEATLK 

      1870       1880       1890       1900       1910       1920 
YEAQISDLEK YISQQELEMK KSIRDNSSYR DKVQEMAQEI EFWKSRYEST MIGSKNIDSN 


NAQSKIFS

« Hide

References

« Hide 'large scale' references
[1]"The MYO1 gene from Saccharomyces cerevisiae: its complete nucleotide sequence."
Sweeney F.P., Watts F.Z., Pocklington M.J., Orr E.
Nucleic Acids Res. 18:7147-7147(1990) [PubMed: 2263482] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The yeast MYO1 gene encoding a myosin-like protein required for cell division."
Watts F.Z., Shiels G., Orr E.
EMBO J. 6:3499-3505(1987) [PubMed: 3322809] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-760.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1476 AND THR-1477, MASS SPECTROMETRY.
[7]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1171, MASS SPECTROMETRY.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1171 AND SER-1206, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X53947 Genomic DNA. Translation: CAA37894.1.
U10399 Genomic DNA. Translation: AAB68872.1.
X06187 Genomic DNA. Translation: CAA29550.1.
BK006934 Genomic DNA. Translation: DAA06714.1.
PIRS46773.
RefSeqNP_011888.1. NM_001179153.1.

3D structure databases

ProteinModelPortalP08964.
SMRP08964. Positions 6-822, 851-976.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-808N.
IntActP08964. 24 interactions.
MINTMINT-582338.
STRINGP08964.

Proteomic databases

PeptideAtlasP08964.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR023W; YHR023W; YHR023W.
GeneID856418.
KEGGsce:YHR023W.
NMPDRfig|4932.3.peg.3034.

Organism-specific databases

CYGDYHR023w.
SGDS000001065. MYO1.

Phylogenomic databases

eggNOGfuNOG04687.
GeneTreeEFGT00050000000497.
HOGENOMHBG397279.
OMARIQANAR.
OrthoDBEOG42591S.

Gene expression databases

ArrayExpressP08964.
GenevestigatorP08964.
GermOnlineYHR023W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
[Graphical view]
KOK10352.
PfamPF00063. Myosin_head. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
PROSITEPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981982.

Entry information

Entry nameMYO1_YEAST
AccessionPrimary (citable) accession number: P08964
Secondary accession number(s): D3DKX0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 1995
Last modified: December 14, 2011
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents