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Protein

Myosin-1

Gene

MYO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for cell division.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi180 – 1878ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • microfilament motor activity Source: SGD

GO - Biological processi

  • actomyosin contractile ring assembly Source: SGD
  • actomyosin contractile ring contraction Source: SGD
  • cell separation after cytokinesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31084-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-1
Alternative name(s):
Type II myosin
Gene namesi
Name:MYO1
Ordered Locus Names:YHR023W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR023W.
SGDiS000001065. MYO1.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • incipient cellular bud site Source: SGD
  • myosin II complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19281928Myosin-1PRO_0000123484Add
BLAST

Proteomic databases

MaxQBiP08964.
PeptideAtlasiP08964.
PRIDEiP08964.

PTM databases

iPTMnetiP08964.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MLC1P531413EBI-11650,EBI-10988

Protein-protein interaction databases

BioGridi36454. 136 interactions.
DIPiDIP-808N.
IntActiP08964. 22 interactions.
MINTiMINT-582338.

Structurei

3D structure databases

ProteinModelPortaliP08964.
SMRiP08964. Positions 8-992.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini75 – 791717Myosin motorAdd
BLAST
Domaini794 – 82330IQPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni460 – 52970Actin-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili856 – 19111056Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00760000118919.
HOGENOMiHOG000248744.
InParanoidiP08964.
KOiK10352.
OrthoDBiEOG77T1CS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08964-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGGQSCSSN MIVWIPDEKE VFVKGELMST DINKNKFTGQ EEQIGIVHPL
60 70 80 90 100
DSTEVSNLVQ VRISDVFPVN PSTFDKVENM SELTHLNEPS VLYNLEKRYD
110 120 130 140 150
CDLIYTYSGL FLVAINPYHN LNLYSEDHIN LYHNKHNRLS KSRLDENSHE
160 170 180 190 200
KLPPHIFAIA EEAYENLLSE GKDQSILVTG ESGAGKTENT KKILQYLASI
210 220 230 240 250
TSGSPSNIAP VSGSSIVESF EMKILQSNPI LESFGNAQTV RNNNSSRFGK
260 270 280 290 300
FIKIEFNEHG MINGAHIEWY LLEKSRIVHQ NSKERNYHIF YQLLSGLDDS
310 320 330 340 350
ELKNLRLKSR NVKDYKILSN SNQDIIPGIN DVENFKELLS ALNIIGFSKD
360 370 380 390 400
QIRWIFQVVA IILLIGNIEF VSDRAEQASF KNDVSAICSN LGVDEKDFQT
410 420 430 440 450
AILRPRSKAG KEWVSQSKNS QQAKFILNAL SRNLYERLFG YIVDMINKNL
460 470 480 490 500
DHGSATLNYI GLLDIAGFEI FENNSFEQLC INYTNEKLQQ FFNNHMFVLE
510 520 530 540 550
QSEYLKENIQ WDYIDYGKDL QLTIDLIESK GPPTGVLPLL DEEAVLPKST
560 570 580 590 600
DESFYSKLIS TWDQNSSKFK RSRLKNGFIL KHYAGDVEYT VEGWLSKNKD
610 620 630 640 650
PLNDNLLSLL SSSQNDIISK LFQPEGEKSS SAGVEANISN QEVKKSARTS
660 670 680 690 700
TFKTTSSRHR EQQITLLNQL ASTHPHFVRC IIPNNVKKVK TFNRRLILDQ
710 720 730 740 750
LRCNGVLEGI RLAREGYPNR IAFQEFFQRY RILYPENSTT TTFSSKLKAS
760 770 780 790 800
TKQNCEFLLT SLQLDTKVYK IGNTKLFFKA GVLADLEKQK DVKLNNIMIK
810 820 830 840 850
LTATIRGYTV RKEITYHLQK LKKTRVIGNT FRLYNRLVKE DPWFNLFIRI
860 870 880 890 900
KPLLTSSNDM TRTKKFNEQI NKLKNDLQEM ESKKKFLEEK NQKTVNELEN
910 920 930 940 950
TQDLLNQEKE NLRKNESLLN RVKTSSETLQ KQFDDLVSEK DEISREKLEV
960 970 980 990 1000
AQNLEEAHQK IQGLQETIRE REATLEKLHS KNNELIKQIS DLNCDISKEQ
1010 1020 1030 1040 1050
SSQSLIKESK LKLENEIKRL KDVINSKEEE IKSFNDKLSS SEEDLDIKLV
1060 1070 1080 1090 1100
TLEKNCNIAM SRLQSLVTEN SDLRSKNENF KKEKAALNNQ LKNKESELLK
1110 1120 1130 1140 1150
MKEKIDNHKK ELATFSKQRD DAVSEHGKIT AELKETRIQL TEYKSNYQKI
1160 1170 1180 1190 1200
KEEYSNFQRE TKEQEQKKRN SLVESLNDSK IKELEARLSQ EISLNQYLNK
1210 1220 1230 1240 1250
RISGNSVETN ISSTRRSTSY SDDPLDKEDI IKKYYDLQLA FTEITRNLEN
1260 1270 1280 1290 1300
EIEEKKNLIS RLRFTETRLA SSSFEDQKIK AQMKKLKKLI QDMDPSIPLD
1310 1320 1330 1340 1350
SILNEPLDNC PDKESDINKL MLEVDYLKRQ LDIETRAHYD AENAISALHS
1360 1370 1380 1390 1400
KFRKIQGESS LSSSDIYKLK FEASEERVKS LEDKLKTMPL RDRTNLPVGD
1410 1420 1430 1440 1450
IIKNRDSISK YEEEIRYYKL ENYKLQEILN ESNGKLSQLT LDLRQSKSKE
1460 1470 1480 1490 1500
ALLSEQLDRL QKDLESTERQ KELLSSTIKQ QKQQFENCMD DLQGNELRLR
1510 1520 1530 1540 1550
EHIHALKQAE EDVKNMASII EKLKTQNKQK EKLIWEREME RNDSDMQLQE
1560 1570 1580 1590 1600
TLLELKRVQD VKKILSDDLA HLKERLSAVE DRSQYTDEIN RLKEELNCSL
1610 1620 1630 1640 1650
KAETNLKKEF ATLKYKLETS TNDSEAKISD LLKQLDHYTK VVEMLNNEKD
1660 1670 1680 1690 1700
AISLAEKELY QKYEALNTEC ESLKGKIVSL TKIKQELESD LNQKTDALQI
1710 1720 1730 1740 1750
SNAALSSSTQ KNKEITEKIK YLEETLQLQM EQNSRNGELV KTLQASCNGY
1760 1770 1780 1790 1800
KDKFDDEKQK NIDLYEENQT LQKLNTDLQL QLKNLHERLS DTTEKNAWLS
1810 1820 1830 1840 1850
KIHELENMVS LETDLKYEEM KKNKSLERAV EELQTKNSQQ TDVIELANKN
1860 1870 1880 1890 1900
RSEFEEATLK YEAQISDLEK YISQQELEMK KSIRDNSSYR DKVQEMAQEI
1910 1920
EFWKSRYEST MIGSKNIDSN NAQSKIFS
Length:1,928
Mass (Da):223,636
Last modified:February 1, 1995 - v3
Checksum:i6F54C7611F43DC9F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361K → I in CAA37894 (PubMed:2263482).Curated
Sequence conflicti46 – 461I → T in CAA37894 (PubMed:2263482).Curated
Sequence conflicti46 – 461I → T in CAA29550 (PubMed:3322809).Curated
Sequence conflicti59 – 591V → S in CAA37894 (PubMed:2263482).Curated
Sequence conflicti59 – 591V → S in CAA29550 (PubMed:3322809).Curated
Sequence conflicti86 – 861L → F in CAA37894 (PubMed:2263482).Curated
Sequence conflicti330 – 3301Missing in CAA37894 (PubMed:2263482).Curated
Sequence conflicti330 – 3301Missing in CAA29550 (PubMed:3322809).Curated
Sequence conflicti343 – 3431N → S in CAA37894 (PubMed:2263482).Curated
Sequence conflicti343 – 3431N → S in CAA29550 (PubMed:3322809).Curated
Sequence conflicti421 – 4266QQAKFI → TKLSSL in CAA37894 (PubMed:2263482).Curated
Sequence conflicti515 – 5151D → S in CAA37894 (PubMed:2263482).Curated
Sequence conflicti529 – 5357SKGPPTG → ARGHDR in CAA37894 (PubMed:2263482).Curated
Sequence conflicti529 – 5357SKGPPTG → ARGHDR in CAA29550 (PubMed:3322809).Curated
Sequence conflicti541 – 5411D → V in CAA37894 (PubMed:2263482).Curated
Sequence conflicti541 – 5411D → V in CAA29550 (PubMed:3322809).Curated
Sequence conflicti550 – 5512TD → LM in CAA37894 (PubMed:2263482).Curated
Sequence conflicti573 – 5731R → A in CAA37894 (PubMed:2263482).Curated
Sequence conflicti582 – 5821H → D in CAA37894 (PubMed:2263482).Curated
Sequence conflicti588 – 59912EYTVE…LSKNK → NTLWKAGYPKT in CAA37894 (PubMed:2263482).CuratedAdd
BLAST
Sequence conflicti599 – 5991Missing in CAA29550 (PubMed:3322809).Curated
Sequence conflicti627 – 6326EKSSSA → GKNLLVC in CAA37894 (PubMed:2263482).Curated
Sequence conflicti627 – 6326EKSSSA → GKNLLVC in CAA29550 (PubMed:3322809).Curated
Sequence conflicti695 – 6951R → S in CAA37894 (PubMed:2263482).Curated
Sequence conflicti695 – 6951R → S in CAA29550 (PubMed:3322809).Curated
Sequence conflicti736 – 7427ENSTTTT → RKFNHHD in CAA29550 (PubMed:3322809).Curated
Sequence conflicti756 – 7561E → R in CAA37894 (PubMed:2263482).Curated
Sequence conflicti756 – 7561E → R in CAA29550 (PubMed:3322809).Curated
Sequence conflicti773 – 78412NTKLF…AGVLA → ILTVFQKLEYWS in CAA37894 (PubMed:2263482).CuratedAdd
BLAST
Sequence conflicti793 – 7942KL → NV in CAA37894 (PubMed:2263482).Curated
Sequence conflicti896 – 8961N → T in CAA37894 (PubMed:2263482).Curated
Sequence conflicti900 – 9001N → NSQITKINTNITETPQSTYI GERPKRVICGN in CAA37894 (PubMed:2263482).Curated
Sequence conflicti906 – 9061N → I in CAA37894 (PubMed:2263482).Curated
Sequence conflicti911 – 9111N → K in CAA37894 (PubMed:2263482).Curated
Sequence conflicti915 – 93016NESLL…SETLQ → RIAIKILKPAINIT in CAA37894 (PubMed:2263482).CuratedAdd
BLAST
Sequence conflicti934 – 9396DDLVSE → MTLFL in CAA37894 (PubMed:2263482).Curated
Sequence conflicti951 – 9588AQNLEEAH → RKILKKLD in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1002 – 10021S → C in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1049 – 10491L → D in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1056 – 10561C → S in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1060 – 10601M → I in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1085 – 10851A → E in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1123 – 11231V → C in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1133 – 11331L → S in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1144 – 11463KSN → NLI in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1159 – 116810RETKEQEQKK → TRKKEEQDKE in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1179 – 120426SKIKE…KRISG → ELKVKEWKARCHRKYLKSIL KQKNIR in CAA37894 (PubMed:2263482).CuratedAdd
BLAST
Sequence conflicti1224 – 12241P → S in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1228 – 12281E → Q in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1253 – 12531E → Q in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1311 – 132313PDKES…KLMLE → LTKSLILTNGNAS in CAA37894 (PubMed:2263482).CuratedAdd
BLAST
Sequence conflicti1400 – 14001D → H in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1454 – 1554101SEQLD…ETLLE → P in CAA37894 (PubMed:2263482).CuratedAdd
BLAST
Sequence conflicti1568 – 15681D → V in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1630 – 164617DLLKQ…VEMLN → SEAARSLYKSGGNVD in CAA37894 (PubMed:2263482).CuratedAdd
BLAST
Sequence conflicti1699 – 17057Missing in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1725 – 173713TLQLQ…NSRNG → NTTANGTKFKEW in CAA37894 (PubMed:2263482).CuratedAdd
BLAST
Sequence conflicti1754 – 17574FDDE → LMM in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1777 – 17771D → E in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1788 – 17881R → T in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1825 – 18251S → D in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1882 – 18821S → W in CAA37894 (PubMed:2263482).Curated
Sequence conflicti1902 – 19043FWK → NSGKRLDADDL in CAA37894 (PubMed:2263482).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53947 Genomic DNA. Translation: CAA37894.1.
U10399 Genomic DNA. Translation: AAB68872.1.
X06187 Genomic DNA. Translation: CAA29550.1.
BK006934 Genomic DNA. Translation: DAA06714.1.
PIRiS46773.
RefSeqiNP_011888.1. NM_001179153.1.

Genome annotation databases

EnsemblFungiiYHR023W; YHR023W; YHR023W.
GeneIDi856418.
KEGGisce:YHR023W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53947 Genomic DNA. Translation: CAA37894.1.
U10399 Genomic DNA. Translation: AAB68872.1.
X06187 Genomic DNA. Translation: CAA29550.1.
BK006934 Genomic DNA. Translation: DAA06714.1.
PIRiS46773.
RefSeqiNP_011888.1. NM_001179153.1.

3D structure databases

ProteinModelPortaliP08964.
SMRiP08964. Positions 8-992.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36454. 136 interactions.
DIPiDIP-808N.
IntActiP08964. 22 interactions.
MINTiMINT-582338.

PTM databases

iPTMnetiP08964.

Proteomic databases

MaxQBiP08964.
PeptideAtlasiP08964.
PRIDEiP08964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR023W; YHR023W; YHR023W.
GeneIDi856418.
KEGGisce:YHR023W.

Organism-specific databases

EuPathDBiFungiDB:YHR023W.
SGDiS000001065. MYO1.

Phylogenomic databases

GeneTreeiENSGT00760000118919.
HOGENOMiHOG000248744.
InParanoidiP08964.
KOiK10352.
OrthoDBiEOG77T1CS.

Enzyme and pathway databases

BioCyciYEAST:G3O-31084-MONOMER.

Miscellaneous databases

PROiP08964.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The MYO1 gene from Saccharomyces cerevisiae: its complete nucleotide sequence."
    Sweeney F.P., Watts F.Z., Pocklington M.J., Orr E.
    Nucleic Acids Res. 18:7147-7147(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The yeast MYO1 gene encoding a myosin-like protein required for cell division."
    Watts F.Z., Shiels G., Orr E.
    EMBO J. 6:3499-3505(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-760.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMYO1_YEAST
AccessioniPrimary (citable) accession number: P08964
Secondary accession number(s): D3DKX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 165 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.