Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type I restriction enzyme EcoKI R protein

Gene

hsdR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The EcoKI enzyme recognizes 5'-AACN6GTGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification.

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.

Catalytic activityi

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi431 – 450H-T-H motifBy similarityAdd BLAST20
Nucleotide bindingi472 – 478ATPPROSITE-ProRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processRestriction system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10459-MONOMER
MetaCyc:EG10459-MONOMER

Protein family/group databases

REBASEi980 EcoKI
TCDBi3.A.17.1.1 the phage t7 injectisome (t7 injectisome) family

Names & Taxonomyi

Protein namesi
Recommended name:
Type I restriction enzyme EcoKI R protein (EC:3.1.21.3)
Short name:
R.EcoKI
Gene namesi
Name:hsdR
Synonyms:hsr
Ordered Locus Names:b4350, JW4313
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10459 hsdR

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000772591 – 1170Type I restriction enzyme EcoKI R proteinAdd BLAST1170

Proteomic databases

PaxDbiP08956
PRIDEiP08956

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Protein-protein interaction databases

BioGridi4262769, 27 interactors
DIPiDIP-9944N
IntActiP08956, 16 interactors
STRINGi316407.85677090

Structurei

3D structure databases

ProteinModelPortaliP08956
SMRiP08956
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini458 – 639Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST182
Domaini714 – 879Helicase C-terminalPROSITE-ProRule annotationAdd BLAST166

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili143 – 229Sequence analysisAdd BLAST87

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi574 – 577DEAH box4

Sequence similaritiesi

Belongs to the HsdR family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4107SCJ Bacteria
COG4096 LUCA
HOGENOMiHOG000295053
InParanoidiP08956
KOiK01153
PhylomeDBiP08956

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR013670 EcoEI_R_C_dom
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR007409 Restrct_endonuc_type1_HsdR_N
PfamiView protein in Pfam
PF08463 EcoEI_R_C, 1 hit
PF00271 Helicase_C, 1 hit
PF04313 HSDR_N, 1 hit
PF04851 ResIII, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

P08956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNKSNFEFL KGVNDFTYAI ACAAENNYPD DPNTTLIKMR MFGEATAKHL
60 70 80 90 100
GLLLNIPPCE NQHDLLRELG KIAFVDDNIL SVFHKLRRIG NQAVHEYHND
110 120 130 140 150
LNDAQMCLRL GFRLAVWYYR LVTKDYDFPV PVFVLPERGE NLYHQEVLTL
160 170 180 190 200
KQQLEQQVRE KAQTQAEVEA QQQKLVALNG YIAILEGKQQ ETEAQTQARL
210 220 230 240 250
AALEAQLAEK NAELAKQTEQ ERKAYHKEIT DQAIKRTLNL SEEESRFLID
260 270 280 290 300
AQLRKAGWQA DSKTLRFSKG ARPEPGVNKA IAEWPTGKDE TGNQGFADYV
310 320 330 340 350
LFVGLKPIAV VEAKRNNIDV PARLNESYRY SKCFDNGFLR ETLLEHYSPD
360 370 380 390 400
EVHEAVPEYE TSWQDTSGKQ RFKIPFCYST NGREYRATMK TKSGIWYRDV
410 420 430 440 450
RDTRNMSKAL PEWHRPEELL EMLGSEPQKQ NQWFADNPGM SELGLRYYQE
460 470 480 490 500
DAVRAVEKAI VKGQQEILLA MATGTGKTRT AIAMMFRLIQ SQRFKRILFL
510 520 530 540 550
VDRRSLGEQA LGAFEDTRIN GDTFNSIFDI KGLTDKFPED STKIHVATVQ
560 570 580 590 600
SLVKRTLQSD EPMPVARYDC IVVDEAHRGY ILDKEQTEGE LQFRSQLDYV
610 620 630 640 650
SAYRRILDHF DAVKIALTAT PALHTVQIFG EPVYRYTYRT AVIDGFLIDQ
660 670 680 690 700
DPPIQIITRN AQEGVYLSKG EQVERISPQG EVINDTLEDD QDFEVADFNR
710 720 730 740 750
GLVIPAFNRA VCNELTNYLD PTGSQKTLVF CVTNAHADMV VEELRAAFKK
760 770 780 790 800
KYPQLEHDAI IKITGDADKD ARKVQTMITR FNKERLPNIV VTVDLLTTGV
810 820 830 840 850
DIPSICNIVF LRKVRSRILY EQMKGRATRL CPEVNKTSFK IFDCVDIYST
860 870 880 890 900
LESVDTMRPV VVRPKVELQT LVNEITDSET YKITEADGRS FAEHSHEQLV
910 920 930 940 950
AKLQRIIGLA TFNRDRSETI DKQVRRLDEL CQDAAGVNFN GFASRLREKG
960 970 980 990 1000
PHWSAEVFNK LPGFIARLEK LKTDINNLND APIFLDIDDE VVSVKSLYGD
1010 1020 1030 1040 1050
YDTPQDFLEA FDSLVQRSPN AQPALQAVIN RPRDLTRKGL VELQEWFDRQ
1060 1070 1080 1090 1100
HFEESSLRKA WKETRNEDIA ARLIGHIRRA AVGDALKPFE ERVDHALTRI
1110 1120 1130 1140 1150
KGENDWSSEQ LSWLDRLAQA LKEKVVLDDD VFKTGNFHRR GGKAMLQRTF
1160 1170
DDNLDTLLGK FSDYIWDELA
Length:1,170
Mass (Da):134,095
Last modified:November 24, 2009 - v3
Checksum:iC5B90A799C455318
GO

Sequence cautioni

The sequence AAA97247 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE78340 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA29791 differs from that shown. Reason: Frameshift at position 1066.Curated
The sequence CAA29791 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA38116 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti611 – 678DAVKI…ERISP → ECGKNRSHRHPGATYCADFR RAGLPLYLPYRGYRRFSDRP GSAYSDHHPQRAGGGLSLQR RAGRAH in CAA29791 (PubMed:3323532).CuratedAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06545 Genomic DNA Translation: CAA29791.1 Sequence problems.
U14003 Genomic DNA Translation: AAA97247.1 Different initiation.
U00096 Genomic DNA Translation: AAC77306.2
AP009048 Genomic DNA Translation: BAE78340.1 Different initiation.
X54198 Genomic DNA Translation: CAA38116.1 Different initiation.
PIRiS56576 NDECKR
RefSeqiNP_418770.2, NC_000913.3
WP_000981388.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77306; AAC77306; b4350
BAE78340; BAE78340; BAE78340
GeneIDi948878
KEGGiecj:JW4313
eco:b4350
PATRICifig|1411691.4.peg.2336

Similar proteinsi

Entry informationi

Entry nameiT1RK_ECOLI
AccessioniPrimary (citable) accession number: P08956
Secondary accession number(s): Q2M5W6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 24, 2009
Last modified: May 23, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health