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P08956

- T1RK_ECOLI

UniProt

P08956 - T1RK_ECOLI

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Protein

Type I restriction enzyme EcoKI R protein

Gene
hsdR, hsr, b4350, JW4313
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

The EcoKI enzyme recognizes 5'-AACN6GTGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification.

Catalytic activityi

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi431 – 45020H-T-H motif By similarityAdd
BLAST
Nucleotide bindingi472 – 4787ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. helicase activity Source: UniProtKB-KW
  4. Type I site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10459-MONOMER.
ECOL316407:JW4313-MONOMER.
MetaCyc:EG10459-MONOMER.

Protein family/group databases

REBASEi980. EcoKI.
TCDBi3.A.17.1.1. the phage t7 injectisome (t7 injectisome) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Type I restriction enzyme EcoKI R protein (EC:3.1.21.3)
Short name:
R.EcoKI
Gene namesi
Name:hsdR
Synonyms:hsr
Ordered Locus Names:b4350, JW4313
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10459. hsdR.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11701170Type I restriction enzyme EcoKI R proteinPRO_0000077259Add
BLAST

Proteomic databases

PaxDbiP08956.

Expressioni

Gene expression databases

GenevestigatoriP08956.

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Protein-protein interaction databases

DIPiDIP-9944N.
IntActiP08956. 15 interactions.
MINTiMINT-1243362.
STRINGi511145.b4350.

Structurei

3D structure databases

ProteinModelPortaliP08956.
SMRiP08956. Positions 237-843.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini458 – 639182Helicase ATP-bindingAdd
BLAST
Domaini714 – 879166Helicase C-terminalAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili143 – 22987 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi574 – 5774DEAH box

Sequence similaritiesi

Belongs to the HsdR family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG4096.
HOGENOMiHOG000295053.
KOiK01153.
OrthoDBiEOG6MPWQ6.
PhylomeDBiP08956.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR025285. DUF4145.
IPR013670. EcoEI_R_C_dom.
IPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
[Graphical view]
PfamiPF13643. DUF4145. 1 hit.
PF08463. EcoEI_R_C. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08956-1 [UniParc]FASTAAdd to Basket

« Hide

MMNKSNFEFL KGVNDFTYAI ACAAENNYPD DPNTTLIKMR MFGEATAKHL     50
GLLLNIPPCE NQHDLLRELG KIAFVDDNIL SVFHKLRRIG NQAVHEYHND 100
LNDAQMCLRL GFRLAVWYYR LVTKDYDFPV PVFVLPERGE NLYHQEVLTL 150
KQQLEQQVRE KAQTQAEVEA QQQKLVALNG YIAILEGKQQ ETEAQTQARL 200
AALEAQLAEK NAELAKQTEQ ERKAYHKEIT DQAIKRTLNL SEEESRFLID 250
AQLRKAGWQA DSKTLRFSKG ARPEPGVNKA IAEWPTGKDE TGNQGFADYV 300
LFVGLKPIAV VEAKRNNIDV PARLNESYRY SKCFDNGFLR ETLLEHYSPD 350
EVHEAVPEYE TSWQDTSGKQ RFKIPFCYST NGREYRATMK TKSGIWYRDV 400
RDTRNMSKAL PEWHRPEELL EMLGSEPQKQ NQWFADNPGM SELGLRYYQE 450
DAVRAVEKAI VKGQQEILLA MATGTGKTRT AIAMMFRLIQ SQRFKRILFL 500
VDRRSLGEQA LGAFEDTRIN GDTFNSIFDI KGLTDKFPED STKIHVATVQ 550
SLVKRTLQSD EPMPVARYDC IVVDEAHRGY ILDKEQTEGE LQFRSQLDYV 600
SAYRRILDHF DAVKIALTAT PALHTVQIFG EPVYRYTYRT AVIDGFLIDQ 650
DPPIQIITRN AQEGVYLSKG EQVERISPQG EVINDTLEDD QDFEVADFNR 700
GLVIPAFNRA VCNELTNYLD PTGSQKTLVF CVTNAHADMV VEELRAAFKK 750
KYPQLEHDAI IKITGDADKD ARKVQTMITR FNKERLPNIV VTVDLLTTGV 800
DIPSICNIVF LRKVRSRILY EQMKGRATRL CPEVNKTSFK IFDCVDIYST 850
LESVDTMRPV VVRPKVELQT LVNEITDSET YKITEADGRS FAEHSHEQLV 900
AKLQRIIGLA TFNRDRSETI DKQVRRLDEL CQDAAGVNFN GFASRLREKG 950
PHWSAEVFNK LPGFIARLEK LKTDINNLND APIFLDIDDE VVSVKSLYGD 1000
YDTPQDFLEA FDSLVQRSPN AQPALQAVIN RPRDLTRKGL VELQEWFDRQ 1050
HFEESSLRKA WKETRNEDIA ARLIGHIRRA AVGDALKPFE ERVDHALTRI 1100
KGENDWSSEQ LSWLDRLAQA LKEKVVLDDD VFKTGNFHRR GGKAMLQRTF 1150
DDNLDTLLGK FSDYIWDELA 1170
Length:1,170
Mass (Da):134,095
Last modified:November 24, 2009 - v3
Checksum:iC5B90A799C455318
GO

Sequence cautioni

The sequence CAA29791.1 differs from that shown. Reason: Frameshift at position 1066.
The sequence AAA97247.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAE78340.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA29791.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence CAA38116.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti611 – 67868DAVKI…ERISP → ECGKNRSHRHPGATYCADFR RAGLPLYLPYRGYRRFSDRP GSAYSDHHPQRAGGGLSLQR RAGRAH in CAA29791. 1 PublicationAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06545 Genomic DNA. Translation: CAA29791.1. Sequence problems.
U14003 Genomic DNA. Translation: AAA97247.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77306.2.
AP009048 Genomic DNA. Translation: BAE78340.1. Different initiation.
X54198 Genomic DNA. Translation: CAA38116.1. Different initiation.
PIRiS56576. NDECKR.
RefSeqiNP_418770.2. NC_000913.3.
YP_492481.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77306; AAC77306; b4350.
BAE78340; BAE78340; BAE78340.
GeneIDi12933775.
948878.
KEGGiecj:Y75_p4235.
eco:b4350.
PATRICi32124306. VBIEscCol129921_4496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06545 Genomic DNA. Translation: CAA29791.1 . Sequence problems.
U14003 Genomic DNA. Translation: AAA97247.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC77306.2 .
AP009048 Genomic DNA. Translation: BAE78340.1 . Different initiation.
X54198 Genomic DNA. Translation: CAA38116.1 . Different initiation.
PIRi S56576. NDECKR.
RefSeqi NP_418770.2. NC_000913.3.
YP_492481.1. NC_007779.1.

3D structure databases

ProteinModelPortali P08956.
SMRi P08956. Positions 237-843.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9944N.
IntActi P08956. 15 interactions.
MINTi MINT-1243362.
STRINGi 511145.b4350.

Protein family/group databases

REBASEi 980. EcoKI.
TCDBi 3.A.17.1.1. the phage t7 injectisome (t7 injectisome) family.

Proteomic databases

PaxDbi P08956.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77306 ; AAC77306 ; b4350 .
BAE78340 ; BAE78340 ; BAE78340 .
GeneIDi 12933775.
948878.
KEGGi ecj:Y75_p4235.
eco:b4350.
PATRICi 32124306. VBIEscCol129921_4496.

Organism-specific databases

EchoBASEi EB0454.
EcoGenei EG10459. hsdR.

Phylogenomic databases

eggNOGi COG4096.
HOGENOMi HOG000295053.
KOi K01153.
OrthoDBi EOG6MPWQ6.
PhylomeDBi P08956.

Enzyme and pathway databases

BioCyci EcoCyc:EG10459-MONOMER.
ECOL316407:JW4313-MONOMER.
MetaCyc:EG10459-MONOMER.

Miscellaneous databases

PROi P08956.

Gene expression databases

Genevestigatori P08956.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR025285. DUF4145.
IPR013670. EcoEI_R_C_dom.
IPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
[Graphical view ]
Pfami PF13643. DUF4145. 1 hit.
PF08463. EcoEI_R_C. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Organization and sequence of the hsd genes of Escherichia coli K-12."
    Loenen W.A.M., Daniel A.S., Braymer H.D., Murray N.E.
    J. Mol. Biol. 198:159-170(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Characterization and expression of the Escherichia coli Mrr restriction system."
    Waite-Rees P.A., Keating C.J., Moran L.S., Slatko B.E., Hornstra L.J., Benner J.S.
    J. Bacteriol. 173:5207-5219(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
    Strain: K12 / CR63.

Entry informationi

Entry nameiT1RK_ECOLI
AccessioniPrimary (citable) accession number: P08956
Secondary accession number(s): Q2M5W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 24, 2009
Last modified: May 14, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi