Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P08956

- T1RK_ECOLI

UniProt

P08956 - T1RK_ECOLI

Protein

Type I restriction enzyme EcoKI R protein

Gene

hsdR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 3 (24 Nov 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The EcoKI enzyme recognizes 5'-AACN6GTGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi431 – 45020H-T-H motifBy similarityAdd
    BLAST
    Nucleotide bindingi472 – 4787ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. helicase activity Source: UniProtKB-KW
    4. Type I site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10459-MONOMER.
    ECOL316407:JW4313-MONOMER.
    MetaCyc:EG10459-MONOMER.

    Protein family/group databases

    REBASEi980. EcoKI.
    TCDBi3.A.17.1.1. the phage t7 injectisome (t7 injectisome) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type I restriction enzyme EcoKI R protein (EC:3.1.21.3)
    Short name:
    R.EcoKI
    Gene namesi
    Name:hsdR
    Synonyms:hsr
    Ordered Locus Names:b4350, JW4313
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10459. hsdR.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11701170Type I restriction enzyme EcoKI R proteinPRO_0000077259Add
    BLAST

    Proteomic databases

    PaxDbiP08956.

    Expressioni

    Gene expression databases

    GenevestigatoriP08956.

    Interactioni

    Subunit structurei

    The type I restriction/modification system is composed of three polypeptides R, M and S.

    Protein-protein interaction databases

    DIPiDIP-9944N.
    IntActiP08956. 15 interactions.
    MINTiMINT-1243362.
    STRINGi511145.b4350.

    Structurei

    3D structure databases

    ProteinModelPortaliP08956.
    SMRiP08956. Positions 237-843.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini458 – 639182Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini714 – 879166Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili143 – 22987Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi574 – 5774DEAH box

    Sequence similaritiesi

    Belongs to the HsdR family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG4096.
    HOGENOMiHOG000295053.
    KOiK01153.
    OrthoDBiEOG6MPWQ6.
    PhylomeDBiP08956.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR025285. DUF4145.
    IPR013670. EcoEI_R_C_dom.
    IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR007409. Restrct_endonuc_type1_HsdR_N.
    [Graphical view]
    PfamiPF13643. DUF4145. 1 hit.
    PF08463. EcoEI_R_C. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF04313. HSDR_N. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08956-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMNKSNFEFL KGVNDFTYAI ACAAENNYPD DPNTTLIKMR MFGEATAKHL     50
    GLLLNIPPCE NQHDLLRELG KIAFVDDNIL SVFHKLRRIG NQAVHEYHND 100
    LNDAQMCLRL GFRLAVWYYR LVTKDYDFPV PVFVLPERGE NLYHQEVLTL 150
    KQQLEQQVRE KAQTQAEVEA QQQKLVALNG YIAILEGKQQ ETEAQTQARL 200
    AALEAQLAEK NAELAKQTEQ ERKAYHKEIT DQAIKRTLNL SEEESRFLID 250
    AQLRKAGWQA DSKTLRFSKG ARPEPGVNKA IAEWPTGKDE TGNQGFADYV 300
    LFVGLKPIAV VEAKRNNIDV PARLNESYRY SKCFDNGFLR ETLLEHYSPD 350
    EVHEAVPEYE TSWQDTSGKQ RFKIPFCYST NGREYRATMK TKSGIWYRDV 400
    RDTRNMSKAL PEWHRPEELL EMLGSEPQKQ NQWFADNPGM SELGLRYYQE 450
    DAVRAVEKAI VKGQQEILLA MATGTGKTRT AIAMMFRLIQ SQRFKRILFL 500
    VDRRSLGEQA LGAFEDTRIN GDTFNSIFDI KGLTDKFPED STKIHVATVQ 550
    SLVKRTLQSD EPMPVARYDC IVVDEAHRGY ILDKEQTEGE LQFRSQLDYV 600
    SAYRRILDHF DAVKIALTAT PALHTVQIFG EPVYRYTYRT AVIDGFLIDQ 650
    DPPIQIITRN AQEGVYLSKG EQVERISPQG EVINDTLEDD QDFEVADFNR 700
    GLVIPAFNRA VCNELTNYLD PTGSQKTLVF CVTNAHADMV VEELRAAFKK 750
    KYPQLEHDAI IKITGDADKD ARKVQTMITR FNKERLPNIV VTVDLLTTGV 800
    DIPSICNIVF LRKVRSRILY EQMKGRATRL CPEVNKTSFK IFDCVDIYST 850
    LESVDTMRPV VVRPKVELQT LVNEITDSET YKITEADGRS FAEHSHEQLV 900
    AKLQRIIGLA TFNRDRSETI DKQVRRLDEL CQDAAGVNFN GFASRLREKG 950
    PHWSAEVFNK LPGFIARLEK LKTDINNLND APIFLDIDDE VVSVKSLYGD 1000
    YDTPQDFLEA FDSLVQRSPN AQPALQAVIN RPRDLTRKGL VELQEWFDRQ 1050
    HFEESSLRKA WKETRNEDIA ARLIGHIRRA AVGDALKPFE ERVDHALTRI 1100
    KGENDWSSEQ LSWLDRLAQA LKEKVVLDDD VFKTGNFHRR GGKAMLQRTF 1150
    DDNLDTLLGK FSDYIWDELA 1170
    Length:1,170
    Mass (Da):134,095
    Last modified:November 24, 2009 - v3
    Checksum:iC5B90A799C455318
    GO

    Sequence cautioni

    The sequence CAA29791.1 differs from that shown. Reason: Frameshift at position 1066.
    The sequence AAA97247.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAE78340.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA29791.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence CAA38116.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti611 – 67868DAVKI…ERISP → ECGKNRSHRHPGATYCADFR RAGLPLYLPYRGYRRFSDRP GSAYSDHHPQRAGGGLSLQR RAGRAH in CAA29791. (PubMed:3323532)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06545 Genomic DNA. Translation: CAA29791.1. Sequence problems.
    U14003 Genomic DNA. Translation: AAA97247.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC77306.2.
    AP009048 Genomic DNA. Translation: BAE78340.1. Different initiation.
    X54198 Genomic DNA. Translation: CAA38116.1. Different initiation.
    PIRiS56576. NDECKR.
    RefSeqiNP_418770.2. NC_000913.3.
    YP_492481.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77306; AAC77306; b4350.
    BAE78340; BAE78340; BAE78340.
    GeneIDi12933775.
    948878.
    KEGGiecj:Y75_p4235.
    eco:b4350.
    PATRICi32124306. VBIEscCol129921_4496.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06545 Genomic DNA. Translation: CAA29791.1 . Sequence problems.
    U14003 Genomic DNA. Translation: AAA97247.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC77306.2 .
    AP009048 Genomic DNA. Translation: BAE78340.1 . Different initiation.
    X54198 Genomic DNA. Translation: CAA38116.1 . Different initiation.
    PIRi S56576. NDECKR.
    RefSeqi NP_418770.2. NC_000913.3.
    YP_492481.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P08956.
    SMRi P08956. Positions 237-843.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9944N.
    IntActi P08956. 15 interactions.
    MINTi MINT-1243362.
    STRINGi 511145.b4350.

    Protein family/group databases

    REBASEi 980. EcoKI.
    TCDBi 3.A.17.1.1. the phage t7 injectisome (t7 injectisome) family.

    Proteomic databases

    PaxDbi P08956.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77306 ; AAC77306 ; b4350 .
    BAE78340 ; BAE78340 ; BAE78340 .
    GeneIDi 12933775.
    948878.
    KEGGi ecj:Y75_p4235.
    eco:b4350.
    PATRICi 32124306. VBIEscCol129921_4496.

    Organism-specific databases

    EchoBASEi EB0454.
    EcoGenei EG10459. hsdR.

    Phylogenomic databases

    eggNOGi COG4096.
    HOGENOMi HOG000295053.
    KOi K01153.
    OrthoDBi EOG6MPWQ6.
    PhylomeDBi P08956.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10459-MONOMER.
    ECOL316407:JW4313-MONOMER.
    MetaCyc:EG10459-MONOMER.

    Miscellaneous databases

    PROi P08956.

    Gene expression databases

    Genevestigatori P08956.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR025285. DUF4145.
    IPR013670. EcoEI_R_C_dom.
    IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR007409. Restrct_endonuc_type1_HsdR_N.
    [Graphical view ]
    Pfami PF13643. DUF4145. 1 hit.
    PF08463. EcoEI_R_C. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF04313. HSDR_N. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Organization and sequence of the hsd genes of Escherichia coli K-12."
      Loenen W.A.M., Daniel A.S., Braymer H.D., Murray N.E.
      J. Mol. Biol. 198:159-170(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Characterization and expression of the Escherichia coli Mrr restriction system."
      Waite-Rees P.A., Keating C.J., Moran L.S., Slatko B.E., Hornstra L.J., Benner J.S.
      J. Bacteriol. 173:5207-5219(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
      Strain: K12 / CR63.

    Entry informationi

    Entry nameiT1RK_ECOLI
    AccessioniPrimary (citable) accession number: P08956
    Secondary accession number(s): Q2M5W6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 139 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3