P08949 (NMB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuromedin-B

Cleaved into the following 2 chains:

1. Neuromedin-B-32
2. Neuromedin-B

Gene names

Name:

NMB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates smooth muscle contraction in a manner similar to that of bombesin.
Subcellular location	Secreted.
Sequence similarities	Belongs to the bombesin/neuromedin-B/ranatensin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
PTM	Amidation Cleavage on pair of basic residues
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adult feeding behavior Inferred from electronic annotation. Source: Compara arachidonic acid secretion Inferred from electronic annotation. Source: Compara cell-cell signaling Traceable author statement Ref.1. Source: ProtInc elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara glucose homeostasis Inferred from electronic annotation. Source: Compara negative regulation of hormone secretion Inferred from electronic annotation. Source: Compara neuropeptide signaling pathway Inferred from electronic annotation. Source: InterPro positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of hormone secretion Inferred from electronic annotation. Source: Compara signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	extracellular region Traceable author statement. Source: Reactome neuron projection Inferred from electronic annotation. Source: Compara
Molecular_function	hormone activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P08949-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P08949-2)

The sequence of this isoform differs from the canonical sequence as follows:
     111-121: YRRLLVQILQK → EAAGTNTAEMTPIMGQTQQRGLDCAHPGKVLNGTLLMAPSGCKS

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Peptide

25 – 56

32

Neuromedin-B-32

PRO_0000003017

Peptide

47 – 56

10

Neuromedin-B

PRO_0000003018

Propeptide

60 – 121

62

PRO_0000003019

Amino acid modifications

Modified residue

56

1

Methionine amide

Natural variations

Alternative sequence

111 – 121

11

YRRLLVQILQK → EAAGTNTAEMTPIMGQTQQR GLDCAHPGKVLNGTLLMAPS GCKS in isoform 2.

VSP_000548

Natural variant

73

1

P → T. Ref.4
Corresponds to variant rs1051168 [ dbSNP | Ensembl ].

VAR_060369

Experimental info

Sequence conflict

68 – 76

9

PLGTAPHTS → HWGQLPTPP in AAA59934. Ref.1

Secondary structure

1

.

.

.

121

Helix Strand Turn

Details...

Helix

51 – 54

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 15, 2009. Version 4. Checksum: 10E16BA4886F03FB	FASTA	121	13,252
	10 20 30 40 50 60 MARRAGGARM FGSLLLFALL AAGVAPLSWD LPEPRSRASK IRVHSRGNLW ATGHFMGKKS 70 80 90 100 110 120 LEPSSPSPLG TAPHTSLRDQ RLQLSHDLLG ILLLKKALGV SLSRPAPQIQ YRRLLVQILQ K
	Isoform 2 [UniParc]. Checksum: 3FFBCF2BB83E2D84	FASTA	154	16,309
	10 20 30 40 50 60 MARRAGGARM FGSLLLFALL AAGVAPLSWD LPEPRSRASK IRVHSRGNLW ATGHFMGKKS 70 80 90 100 110 120 LEPSSPSPLG TAPHTSLRDQ RLQLSHDLLG ILLLKKALGV SLSRPAPQIQ EAAGTNTAEM 130 140 150 TPIMGQTQQR GLDCAHPGKV LNGTLLMAPS GCKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of cDNAs encoding the human bombesin-like peptide neuromedin B. Chromosomal localization and comparison to cDNAs encoding its amphibian homolog ranatensin." Krane I.M., Naylor S.L., Helin-Davis D., Chin W.W., Spindel E.R. J. Biol. Chem. 263:13317-13323(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Hypothalamus.
[2]	Erratum Krane I.M., Naylor S.L., Helin-Davis D., Chin W.W., Spindel E.R. J. Biol. Chem. 265:7091-7091(1990) Cited for: SEQUENCE REVISION.
[3]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT THR-73. Tissue: Brain and Ovary.
[5]	"Solution structure of neuromedin B by (1)H nuclear magnetic resonance spectroscopy." Lee S., Kim Y. FEBS Lett. 460:263-269(1999) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 47-56.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M21551 mRNA. Translation: AAA59934.1.
AC048382 Genomic DNA. No translation available.
BC007407 mRNA. Translation: AAH07407.1.
BC007431 mRNA. Translation: AAH07431.1.
BC008603 mRNA. Translation: AAH08603.1.

IPI

IPI00031753.
IPI00220630.

PIR

A28945.

RefSeq

NP_066563.2. NM_021077.3.
NP_995580.1. NM_205858.1.

UniGene

Hs.386470.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C98	NMR	-	A	47-56	[»]
1C9A	NMR	-	A	47-56	[»]

ProteinModelPortal

P08949.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000378089.

Polymorphism databases

DMDM

281185514.

Proteomic databases

PaxDb

P08949.

PRIDE

P08949.

Protocols and materials databases

DNASU

4828.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000360476; ENSP00000353664; ENSG00000197696.
ENST00000394588; ENSP00000378089; ENSG00000197696.

GeneID

4828.

KEGG

hsa:4828.

UCSC

uc002bkz.3. human.
uc002bla.3. human.

Organism-specific databases

CTD

4828.

GeneCards

GC15M085198.

H-InvDB

HIX0202167.

HGNC

HGNC:7842. NMB.

HPA

CAB019302.

MIM

162340. gene.

neXtProt

NX_P08949.

PharmGKB

PA31654.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG40699.

HOGENOM

HOG000063661.

HOVERGEN

HBG079570.

KO

K05223.

OMA

LGTAPHT.

OrthoDB

EOG4X97JR.

PhylomeDB

P08949.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P08949.

CleanEx

HS_NMB.

Genevestigator

P08949.

GermOnline

ENSG00000197696. Homo sapiens.

Family and domain databases

InterPro

IPR000874. Bombesin.
[Graphical view]

Pfam

PF02044. Bombesin. 1 hit.
[Graphical view]

PROSITE

PS00257. BOMBESIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

NMB. human.

EvolutionaryTrace

P08949.

GenomeRNAi

4828.

NextBio

18596.

SOURCE

Search...

Entry information

Entry name

NMB_HUMAN

Accession

Primary (citable) accession number: P08949
Secondary accession number(s): Q96A06, Q96HH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	December 15, 2009
Last modified:	April 3, 2013
This is version 131 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.