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P08932

- KNT2_RAT

UniProt

P08932 - KNT2_RAT

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Protein
T-kininogen 2
Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Kininogens are plasma glycoproteins with a number of functions: (1) as precursor of the active peptide bradykinin they effect smooth muscle contraction, induction of hypotension and increase of vascular permeability. (2) They play a role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. (3) They are inhibitor of thiol proteases.

GO - Molecular functioni

  1. cysteine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. acute-phase response Source: UniProtKB-KW
  2. vasodilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor, Vasoactive, Vasodilator

Keywords - Biological processi

Acute phase

Protein family/group databases

MEROPSiI25.018.

Names & Taxonomyi

Protein namesi
Recommended name:
T-kininogen 2
Alternative name(s):
Alpha-1-MAP
Major acute phase protein
T-kininogen II
Thiostatin
Cleaved into the following 3 chains:
Alternative name(s):
T-kininogen II heavy chain
Alternative name(s):
T-kininogen II light chain
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818
Add
BLAST
Chaini19 – 430412T-kininogen 2
PRO_0000006703Add
BLAST
Chaini19 – 375357T-kininogen 2 heavy chain
PRO_0000006704Add
BLAST
Peptidei376 – 38611T-kinin
PRO_0000006705Add
BLAST
Chaini387 – 43044T-kininogen 2 light chain
PRO_0000006706Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Pyrrolidone carboxylic acid By similarity
Disulfide bondi28 ↔ 404Interchain (between heavy and light chains) By similarity
Glycosylationi82 – 821N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi83 ↔ 94 By similarity
Disulfide bondi107 ↔ 125 By similarity
Glycosylationi126 – 1261N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi141 ↔ 144 By similarity
Glycosylationi168 – 1681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi204 – 2041N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi205 ↔ 217 By similarity
Disulfide bondi228 ↔ 247 By similarity
Disulfide bondi263 ↔ 266 By similarity
Glycosylationi326 – 3261N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi327 ↔ 339 By similarity
Disulfide bondi350 ↔ 369 By similarity

Post-translational modificationi

As T-kinin is preceded by a Met instead of an Arg or Lys, it is not released from its precursor by either tissue or plasma kallikrein.

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP08932.
PRIDEiP08932.

PTM databases

UniCarbKBiP08932.

Expressioni

Tissue specificityi

Plasma.

Inductioni

In response to an inflammatory stimulant. T-kininogen II synthesis is induced and the plasma concentration of T-kininogen I is raised.

Gene expression databases

ArrayExpressiP08932.
GenevestigatoriP08932.

Structurei

3D structure databases

ProteinModelPortaliP08932.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 131104Cystatin kininogen-type 1
Add
BLAST
Domaini150 – 253104Cystatin kininogen-type 2
Add
BLAST
Domaini272 – 375104Cystatin kininogen-type 3
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG72605.
HOGENOMiHOG000113239.
HOVERGENiHBG006224.
PhylomeDBiP08932.
TreeFamiTF351852.

Family and domain databases

InterProiIPR027358. Kininogen-type_cystatin_dom.
IPR000010. Prot_inh_cystat.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PfamiPF00031. Cystatin. 3 hits.
[Graphical view]
SMARTiSM00043. CY. 3 hits.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 2 hits.
PS51647. CYSTATIN_KININOGEN. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08932-1 [UniParc]FASTAAdd to Basket

« Hide

MKLITILLLC SRLLPSLAQE EGAQEMDCND ETVFQAVDTA LKKYNAELES    50
GNQFLLYRVT EGTKKDGAET LYSFKYQIKE GNCSVQSGLT WQDCDFKDAE 100
EAATGECTTT LGKKENKFSV ATQICNITPG KGPKKTEEDL CVGCFQPIPM 150
DSSDLKPVLK HAVEHFNNNT KHTHLFALTE VKSAHSQVVA GMNYKIIYSI 200
VQTNCSKEDF PFLREDCVPL PYGDHGECRG HTYVDIHNTI AGFSQSCDLY 250
PGDDLFSLLP KKCFGCPKNI PVDSPELKEA LGHSIAQLNA QHNHLFYFKI 300
DTVKKATSQV VAGTKYVIEF IARETNCSKQ TNTELTADCE TKHLGQSLNC 350
NANVYMRPWE NKVVPTVRCQ ALDMMISRPP GFSPFRLVQV QETKEGTTRL 400
LNSCEYKGRL SKAGAGPAPD HQAEASTVTP 430
Length:430
Mass (Da):47,704
Last modified:February 1, 2005 - v2
Checksum:iD94628D848C81525
GO

Sequence cautioni

The sequence AAA41570.1 differs from that shown. Reason: Frameshift at positions 180 and 181.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 272MD → LN AA sequence 1 Publication
Sequence conflicti28 – 281C → R in AAA41570. 1 Publication
Sequence conflicti55 – 551L → V AA sequence 1 Publication
Sequence conflicti61 – 611E → K AA sequence 1 Publication
Sequence conflicti166 – 1661F → S AA sequence 1 Publication
Sequence conflicti179 – 1791T → R AA sequence 1 Publication
Sequence conflicti193 – 1931N → D in AAA41570. 1 Publication
Sequence conflicti212 – 2121F → S AA sequence 1 Publication
Sequence conflicti229 – 2291R → T AA sequence 1 Publication
Sequence conflicti233 – 2331Y → H AA sequence 1 Publication
Sequence conflicti415 – 4151A → L in AAA41570. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC088161 mRNA. Translation: AAH88161.1.
M11661 mRNA. Translation: AAA41570.1. Frameshift.
M11885 mRNA. Translation: AAA41491.1.
PIRiB28055.
RefSeqiNP_001009628.1. NM_001009628.1.
UniGeneiRn.44576.

Genome annotation databases

GeneIDi288001.
KEGGirno:288001.
UCSCiRGD:1359376. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC088161 mRNA. Translation: AAH88161.1 .
M11661 mRNA. Translation: AAA41570.1 . Frameshift.
M11885 mRNA. Translation: AAA41491.1 .
PIRi B28055.
RefSeqi NP_001009628.1. NM_001009628.1.
UniGenei Rn.44576.

3D structure databases

ProteinModelPortali P08932.
ModBasei Search...

Protein family/group databases

MEROPSi I25.018.

PTM databases

UniCarbKBi P08932.

Proteomic databases

PaxDbi P08932.
PRIDEi P08932.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 288001.
KEGGi rno:288001.
UCSCi RGD:1359376. rat.

Organism-specific databases

CTDi 288001.

Phylogenomic databases

eggNOGi NOG72605.
HOGENOMi HOG000113239.
HOVERGENi HBG006224.
PhylomeDBi P08932.
TreeFami TF351852.

Miscellaneous databases

NextBioi 627394.

Gene expression databases

ArrayExpressi P08932.
Genevestigatori P08932.

Family and domain databases

InterProi IPR027358. Kininogen-type_cystatin_dom.
IPR000010. Prot_inh_cystat.
IPR018073. Prot_inh_cystat_CS.
[Graphical view ]
Pfami PF00031. Cystatin. 3 hits.
[Graphical view ]
SMARTi SM00043. CY. 3 hits.
[Graphical view ]
PROSITEi PS00287. CYSTATIN. 2 hits.
PS51647. CYSTATIN_KININOGEN. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  2. "The relationship between rat major acute phase protein and the kininogens."
    Anderson K.P., Heath E.C.
    J. Biol. Chem. 260:12065-12071(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-430, PARTIAL PROTEIN SEQUENCE.
  3. "Primary structures of the mRNAs encoding the rat precursors for bradykinin and T-kinin. Structural relationship of kininogens with major acute phase protein and alpha 1-cysteine proteinase inhibitor."
    Furuto-Kato S., Matsumoto A., Kitamura N., Nakanishi S.
    J. Biol. Chem. 260:12054-12059(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 238-430.

Entry informationi

Entry nameiKNT2_RAT
AccessioniPrimary (citable) accession number: P08932
Secondary accession number(s): Q5M894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Rats express four types of kininogens: the classical HMW and LMW kininogens produced by alternative splicing of the same gene, and two additional LMW-like kininogens: T-I and T-II.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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