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P08932

- KNT2_RAT

UniProt

P08932 - KNT2_RAT

Protein

T-kininogen 2

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Kininogens are plasma glycoproteins with a number of functions: (1) as precursor of the active peptide bradykinin they effect smooth muscle contraction, induction of hypotension and increase of vascular permeability. (2) They play a role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. (3) They are inhibitor of thiol proteases.

    GO - Molecular functioni

    1. cysteine-type endopeptidase inhibitor activity Source: UniProtKB-KW

    GO - Biological processi

    1. acute-phase response Source: UniProtKB-KW
    2. vasodilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Protease inhibitor, Thiol protease inhibitor, Vasoactive, Vasodilator

    Keywords - Biological processi

    Acute phase

    Protein family/group databases

    MEROPSiI25.018.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-kininogen 2
    Alternative name(s):
    Alpha-1-MAP
    Major acute phase protein
    T-kininogen II
    Thiostatin
    Cleaved into the following 3 chains:
    Alternative name(s):
    T-kininogen II heavy chain
    Alternative name(s):
    T-kininogen II light chain
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Chaini19 – 430412T-kininogen 2PRO_0000006703Add
    BLAST
    Chaini19 – 375357T-kininogen 2 heavy chainPRO_0000006704Add
    BLAST
    Peptidei376 – 38611T-kininPRO_0000006705Add
    BLAST
    Chaini387 – 43044T-kininogen 2 light chainPRO_0000006706Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191Pyrrolidone carboxylic acidBy similarity
    Disulfide bondi28 ↔ 404Interchain (between heavy and light chains)PROSITE-ProRule annotation
    Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi83 ↔ 94PROSITE-ProRule annotation
    Disulfide bondi107 ↔ 125PROSITE-ProRule annotation
    Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi141 ↔ 144PROSITE-ProRule annotation
    Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi205 ↔ 217PROSITE-ProRule annotation
    Disulfide bondi228 ↔ 247PROSITE-ProRule annotation
    Disulfide bondi263 ↔ 266PROSITE-ProRule annotation
    Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi327 ↔ 339PROSITE-ProRule annotation
    Disulfide bondi350 ↔ 369PROSITE-ProRule annotation

    Post-translational modificationi

    As T-kinin is preceded by a Met instead of an Arg or Lys, it is not released from its precursor by either tissue or plasma kallikrein.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiP08932.
    PRIDEiP08932.

    PTM databases

    UniCarbKBiP08932.

    Expressioni

    Tissue specificityi

    Plasma.

    Inductioni

    In response to an inflammatory stimulant. T-kininogen II synthesis is induced and the plasma concentration of T-kininogen I is raised.

    Gene expression databases

    ArrayExpressiP08932.
    GenevestigatoriP08932.

    Structurei

    3D structure databases

    ProteinModelPortaliP08932.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 131104Cystatin kininogen-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini150 – 253104Cystatin kininogen-type 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini272 – 375104Cystatin kininogen-type 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 cystatin kininogen-type domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG72605.
    HOGENOMiHOG000113239.
    HOVERGENiHBG006224.
    PhylomeDBiP08932.
    TreeFamiTF351852.

    Family and domain databases

    InterProiIPR027358. Kininogen-type_cystatin_dom.
    IPR000010. Prot_inh_cystat.
    IPR018073. Prot_inh_cystat_CS.
    [Graphical view]
    PfamiPF00031. Cystatin. 3 hits.
    [Graphical view]
    SMARTiSM00043. CY. 3 hits.
    [Graphical view]
    PROSITEiPS00287. CYSTATIN. 2 hits.
    PS51647. CYSTATIN_KININOGEN. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08932-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLITILLLC SRLLPSLAQE EGAQEMDCND ETVFQAVDTA LKKYNAELES    50
    GNQFLLYRVT EGTKKDGAET LYSFKYQIKE GNCSVQSGLT WQDCDFKDAE 100
    EAATGECTTT LGKKENKFSV ATQICNITPG KGPKKTEEDL CVGCFQPIPM 150
    DSSDLKPVLK HAVEHFNNNT KHTHLFALTE VKSAHSQVVA GMNYKIIYSI 200
    VQTNCSKEDF PFLREDCVPL PYGDHGECRG HTYVDIHNTI AGFSQSCDLY 250
    PGDDLFSLLP KKCFGCPKNI PVDSPELKEA LGHSIAQLNA QHNHLFYFKI 300
    DTVKKATSQV VAGTKYVIEF IARETNCSKQ TNTELTADCE TKHLGQSLNC 350
    NANVYMRPWE NKVVPTVRCQ ALDMMISRPP GFSPFRLVQV QETKEGTTRL 400
    LNSCEYKGRL SKAGAGPAPD HQAEASTVTP 430
    Length:430
    Mass (Da):47,704
    Last modified:February 1, 2005 - v2
    Checksum:iD94628D848C81525
    GO

    Sequence cautioni

    The sequence AAA41570.1 differs from that shown. Reason: Frameshift at positions 180 and 181.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 272MD → LN AA sequence (PubMed:2413019)Curated
    Sequence conflicti28 – 281C → R in AAA41570. (PubMed:2413019)Curated
    Sequence conflicti55 – 551L → V AA sequence (PubMed:2413019)Curated
    Sequence conflicti61 – 611E → K AA sequence (PubMed:2413019)Curated
    Sequence conflicti166 – 1661F → S AA sequence (PubMed:2413019)Curated
    Sequence conflicti179 – 1791T → R AA sequence (PubMed:2413019)Curated
    Sequence conflicti193 – 1931N → D in AAA41570. (PubMed:2413019)Curated
    Sequence conflicti212 – 2121F → S AA sequence (PubMed:2413019)Curated
    Sequence conflicti229 – 2291R → T AA sequence (PubMed:2413019)Curated
    Sequence conflicti233 – 2331Y → H AA sequence (PubMed:2413019)Curated
    Sequence conflicti415 – 4151A → L in AAA41570. (PubMed:2413019)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC088161 mRNA. Translation: AAH88161.1.
    M11661 mRNA. Translation: AAA41570.1. Frameshift.
    M11885 mRNA. Translation: AAA41491.1.
    PIRiB28055.
    RefSeqiNP_001009628.1. NM_001009628.1.
    UniGeneiRn.44576.

    Genome annotation databases

    GeneIDi288001.
    KEGGirno:288001.
    UCSCiRGD:1359376. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC088161 mRNA. Translation: AAH88161.1 .
    M11661 mRNA. Translation: AAA41570.1 . Frameshift.
    M11885 mRNA. Translation: AAA41491.1 .
    PIRi B28055.
    RefSeqi NP_001009628.1. NM_001009628.1.
    UniGenei Rn.44576.

    3D structure databases

    ProteinModelPortali P08932.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi I25.018.

    PTM databases

    UniCarbKBi P08932.

    Proteomic databases

    PaxDbi P08932.
    PRIDEi P08932.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 288001.
    KEGGi rno:288001.
    UCSCi RGD:1359376. rat.

    Organism-specific databases

    CTDi 288001.

    Phylogenomic databases

    eggNOGi NOG72605.
    HOGENOMi HOG000113239.
    HOVERGENi HBG006224.
    PhylomeDBi P08932.
    TreeFami TF351852.

    Miscellaneous databases

    NextBioi 627394.

    Gene expression databases

    ArrayExpressi P08932.
    Genevestigatori P08932.

    Family and domain databases

    InterProi IPR027358. Kininogen-type_cystatin_dom.
    IPR000010. Prot_inh_cystat.
    IPR018073. Prot_inh_cystat_CS.
    [Graphical view ]
    Pfami PF00031. Cystatin. 3 hits.
    [Graphical view ]
    SMARTi SM00043. CY. 3 hits.
    [Graphical view ]
    PROSITEi PS00287. CYSTATIN. 2 hits.
    PS51647. CYSTATIN_KININOGEN. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    2. "The relationship between rat major acute phase protein and the kininogens."
      Anderson K.P., Heath E.C.
      J. Biol. Chem. 260:12065-12071(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-430, PARTIAL PROTEIN SEQUENCE.
    3. "Primary structures of the mRNAs encoding the rat precursors for bradykinin and T-kinin. Structural relationship of kininogens with major acute phase protein and alpha 1-cysteine proteinase inhibitor."
      Furuto-Kato S., Matsumoto A., Kitamura N., Nakanishi S.
      J. Biol. Chem. 260:12054-12059(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 238-430.

    Entry informationi

    Entry nameiKNT2_RAT
    AccessioniPrimary (citable) accession number: P08932
    Secondary accession number(s): Q5M894
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Rats express four types of kininogens: the classical HMW and LMW kininogens produced by alternative splicing of the same gene, and two additional LMW-like kininogens: T-I and T-II.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3