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Protein

T-kininogen 2

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Kininogens are plasma glycoproteins with a number of functions: (1) as precursor of the active peptide bradykinin they effect smooth muscle contraction, induction of hypotension and increase of vascular permeability. (2) They play a role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII. (3) They are inhibitor of thiol proteases.

GO - Molecular functioni

  1. cysteine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

  1. acute-phase response Source: UniProtKB-KW
  2. vasodilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor, Vasoactive, Vasodilator

Keywords - Biological processi

Acute phase

Protein family/group databases

MEROPSiI25.018.

Names & Taxonomyi

Protein namesi
Recommended name:
T-kininogen 2
Alternative name(s):
Alpha-1-MAP
Major acute phase protein
T-kininogen II
Thiostatin
Cleaved into the following 3 chains:
Alternative name(s):
T-kininogen II heavy chain
Alternative name(s):
T-kininogen II light chain
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Chaini19 – 430412T-kininogen 2PRO_0000006703Add
BLAST
Chaini19 – 375357T-kininogen 2 heavy chainPRO_0000006704Add
BLAST
Peptidei376 – 38611T-kininPRO_0000006705Add
BLAST
Chaini387 – 43044T-kininogen 2 light chainPRO_0000006706Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Pyrrolidone carboxylic acidBy similarity
Disulfide bondi28 ↔ 404Interchain (between heavy and light chains)PROSITE-ProRule annotation
Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi83 ↔ 94PROSITE-ProRule annotation
Disulfide bondi107 ↔ 125PROSITE-ProRule annotation
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi141 ↔ 144PROSITE-ProRule annotation
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi205 ↔ 217PROSITE-ProRule annotation
Disulfide bondi228 ↔ 247PROSITE-ProRule annotation
Disulfide bondi263 ↔ 266PROSITE-ProRule annotation
Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi327 ↔ 339PROSITE-ProRule annotation
Disulfide bondi350 ↔ 369PROSITE-ProRule annotation

Post-translational modificationi

As T-kinin is preceded by a Met instead of an Arg or Lys, it is not released from its precursor by either tissue or plasma kallikrein.

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP08932.
PRIDEiP08932.

PTM databases

UniCarbKBiP08932.

Expressioni

Tissue specificityi

Plasma.

Inductioni

In response to an inflammatory stimulant. T-kininogen II synthesis is induced and the plasma concentration of T-kininogen I is raised.

Gene expression databases

GenevestigatoriP08932.

Structurei

3D structure databases

ProteinModelPortaliP08932.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 131104Cystatin kininogen-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini150 – 253104Cystatin kininogen-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini272 – 375104Cystatin kininogen-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 cystatin kininogen-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG72605.
HOGENOMiHOG000113239.
HOVERGENiHBG006224.
PhylomeDBiP08932.
TreeFamiTF351852.

Family and domain databases

InterProiIPR027358. Kininogen-type_cystatin_dom.
IPR000010. Prot_inh_cystat.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PfamiPF00031. Cystatin. 3 hits.
[Graphical view]
SMARTiSM00043. CY. 3 hits.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 2 hits.
PS51647. CYSTATIN_KININOGEN. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLITILLLC SRLLPSLAQE EGAQEMDCND ETVFQAVDTA LKKYNAELES
60 70 80 90 100
GNQFLLYRVT EGTKKDGAET LYSFKYQIKE GNCSVQSGLT WQDCDFKDAE
110 120 130 140 150
EAATGECTTT LGKKENKFSV ATQICNITPG KGPKKTEEDL CVGCFQPIPM
160 170 180 190 200
DSSDLKPVLK HAVEHFNNNT KHTHLFALTE VKSAHSQVVA GMNYKIIYSI
210 220 230 240 250
VQTNCSKEDF PFLREDCVPL PYGDHGECRG HTYVDIHNTI AGFSQSCDLY
260 270 280 290 300
PGDDLFSLLP KKCFGCPKNI PVDSPELKEA LGHSIAQLNA QHNHLFYFKI
310 320 330 340 350
DTVKKATSQV VAGTKYVIEF IARETNCSKQ TNTELTADCE TKHLGQSLNC
360 370 380 390 400
NANVYMRPWE NKVVPTVRCQ ALDMMISRPP GFSPFRLVQV QETKEGTTRL
410 420 430
LNSCEYKGRL SKAGAGPAPD HQAEASTVTP
Length:430
Mass (Da):47,704
Last modified:January 31, 2005 - v2
Checksum:iD94628D848C81525
GO

Sequence cautioni

The sequence AAA41570.1 differs from that shown. Reason: Frameshift at positions 180 and 181. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 272MD → LN AA sequence (PubMed:2413019).Curated
Sequence conflicti28 – 281C → R in AAA41570 (PubMed:2413019).Curated
Sequence conflicti55 – 551L → V AA sequence (PubMed:2413019).Curated
Sequence conflicti61 – 611E → K AA sequence (PubMed:2413019).Curated
Sequence conflicti166 – 1661F → S AA sequence (PubMed:2413019).Curated
Sequence conflicti179 – 1791T → R AA sequence (PubMed:2413019).Curated
Sequence conflicti193 – 1931N → D in AAA41570 (PubMed:2413019).Curated
Sequence conflicti212 – 2121F → S AA sequence (PubMed:2413019).Curated
Sequence conflicti229 – 2291R → T AA sequence (PubMed:2413019).Curated
Sequence conflicti233 – 2331Y → H AA sequence (PubMed:2413019).Curated
Sequence conflicti415 – 4151A → L in AAA41570 (PubMed:2413019).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088161 mRNA. Translation: AAH88161.1.
M11661 mRNA. Translation: AAA41570.1. Frameshift.
M11885 mRNA. Translation: AAA41491.1.
PIRiB28055.
RefSeqiNP_001009628.1. NM_001009628.1.
UniGeneiRn.128333.
Rn.44576.

Genome annotation databases

GeneIDi288001.
KEGGirno:288001.
UCSCiRGD:1359376. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088161 mRNA. Translation: AAH88161.1.
M11661 mRNA. Translation: AAA41570.1. Frameshift.
M11885 mRNA. Translation: AAA41491.1.
PIRiB28055.
RefSeqiNP_001009628.1. NM_001009628.1.
UniGeneiRn.128333.
Rn.44576.

3D structure databases

ProteinModelPortaliP08932.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI25.018.

PTM databases

UniCarbKBiP08932.

Proteomic databases

PaxDbiP08932.
PRIDEiP08932.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi288001.
KEGGirno:288001.
UCSCiRGD:1359376. rat.

Organism-specific databases

CTDi288001.

Phylogenomic databases

eggNOGiNOG72605.
HOGENOMiHOG000113239.
HOVERGENiHBG006224.
PhylomeDBiP08932.
TreeFamiTF351852.

Miscellaneous databases

NextBioi627394.

Gene expression databases

GenevestigatoriP08932.

Family and domain databases

InterProiIPR027358. Kininogen-type_cystatin_dom.
IPR000010. Prot_inh_cystat.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PfamiPF00031. Cystatin. 3 hits.
[Graphical view]
SMARTiSM00043. CY. 3 hits.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 2 hits.
PS51647. CYSTATIN_KININOGEN. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  2. "The relationship between rat major acute phase protein and the kininogens."
    Anderson K.P., Heath E.C.
    J. Biol. Chem. 260:12065-12071(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-430, PARTIAL PROTEIN SEQUENCE.
  3. "Primary structures of the mRNAs encoding the rat precursors for bradykinin and T-kinin. Structural relationship of kininogens with major acute phase protein and alpha 1-cysteine proteinase inhibitor."
    Furuto-Kato S., Matsumoto A., Kitamura N., Nakanishi S.
    J. Biol. Chem. 260:12054-12059(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 238-430.

Entry informationi

Entry nameiKNT2_RAT
AccessioniPrimary (citable) accession number: P08932
Secondary accession number(s): Q5M894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1988
Last sequence update: January 31, 2005
Last modified: March 3, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Rats express four types of kininogens: the classical HMW and LMW kininogens produced by alternative splicing of the same gene, and two additional LMW-like kininogens: T-I and T-II.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.