Reviewed, UniProtKB/Swiss-Prot P08928 (LAM0_DROME)

Names and origin

Protein names

Recommended name:
    Lamin Dm0

Gene names

Name:	Lam
ORF Names:	CG6944

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Ref.1
Subunit structure	Interacts directly with LBR. Ref.8
Subcellular location	Nucleus. Note: Nuclear periphery. Ref.7
Tissue specificity	Constitutively expressed in all tissues. Ref.7
Developmental stage	Constitutively expressed in all developmental stages, especially during the first 6-9 hours. Ref.7
Post-translational modification	Three forms of lamin have been identified in D.melanogaster, lamin Dm0 is rapidly processed to lamin Dm1 in the cytoplasm, Dm1 is then assembled in the nuclear envelope and is then phosphorylated, forming lamin Dm2. Ref.9
Sequence similarities	Belongs to the intermediate filament family.

Ontologies

Keywords
Cellular component	Intermediate filament Nucleus
Domain	Coiled coil
PTM	Lipoprotein Phosphoprotein Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	central nervous system development Inferred from mutant phenotype. Source: FlyBase chitin-based cuticle development Inferred from mutant phenotype. Source: FlyBase compound eye morphogenesis Inferred from mutant phenotype. Source: FlyBase digestive tract morphogenesis Inferred from mutant phenotype. Source: FlyBase gonad morphogenesis Inferred from mutant phenotype. Source: FlyBase mitotic nuclear envelope reassembly Inferred from direct assay. Source: FlyBase nuclear migration Inferred from mutant phenotype. Source: FlyBase terminal branching, open tracheal system Inferred from mutant phenotype. Source: FlyBase
Cellular component	lamin filament Traceable author statement. Source: FlyBase lipid particle Inferred from direct assay. Source: FlyBase
Molecular function	receptor binding Ref.8 Inferred from physical interaction. Source: UniProtKB structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ote	P20240	1	EBI-188444,EBI-115143

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	Lamin Dm0		PRO_0000063828
Regions
	Region	1 – 56	56	Head
	Region	55 – 91	37	Coil 1A
	Region	57 – 408	352	Rod
	Region	92 – 103	12	Linker 1
	Region	104 – 241	138	Coil 1B
	Region	242 – 265	24	Linker 2
	Region	266 – 408	143	Coil 2
	Region	409 – 622	214	Tail
	Motif	446 – 451	6	Nuclear localization signal Potential
	Compositional bias	28 – 33	6	Poly-Pro
Sites
	Site	289	1	Heptad change of phase
	Site	353	1	Heptad change of phase
Amino acid modifications
	Modified residue	10	1	Phosphothreonine Ref.9
	Modified residue	12	1	Phosphothreonine Ref.9
	Modified residue	20	1	Phosphothreonine Ref.9
	Modified residue	25	1	Phosphoserine Ref.9
	Modified residue	34	1	Phosphoserine Ref.9
	Modified residue	39	1	Phosphothreonine Ref.9
	Modified residue	41	1	Phosphoserine Ref.9
	Modified residue	42	1	Phosphoserine Ref.9
	Modified residue	45	1	Phosphoserine Ref.9
	Modified residue	47	1	Phosphothreonine Ref.9
	Modified residue	235	1	Phosphoserine Ref.9
	Modified residue	249	1	Phosphotyrosine Ref.9
	Modified residue	250	1	Phosphoserine Ref.9
	Modified residue	311	1	Phosphoserine Ref.9
	Modified residue	413	1	Phosphothreonine Ref.9
	Modified residue	435	1	Phosphothreonine Ref.9
	Modified residue	442	1	Phosphoserine Ref.9
	Modified residue	455	1	Phosphoserine Ref.9
	Modified residue	459	1	Phosphoserine Ref.9
	Modified residue	595	1	Phosphoserine Ref.9
	Modified residue	597	1	Phosphothreonine Ref.9
	Modified residue	615	1	Phosphoserine Ref.9
	Lipidation	619	1	S-farnesyl cysteine By similarity
Experimental info
	Sequence conflict	135	1	E → R in CAA30259. Ref.1
	Sequence conflict	270 – 271	2	EL → DV in CAA30259. Ref.1
	Sequence conflict	290	1	Y → I in CAA30259. Ref.1
	Sequence conflict	290	1	Y → I in CAA34351. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P08928-1 [UniParc]. Last modified October 25, 2005. Version 4. Checksum: B77E48AA5CD9A8BD	FASTA	622	71,300
	10 20 30 40 50 60 MSSKSRRAGT ATPQPGNTST PRPPSAGPQP PPPSTHSQTA SSPLSPTRHS RVAEKVELQN 70 80 90 100 110 120 LNDRLATYID RVRNLETENS RLTIEVQTTR DTVTRETTNI KNIFEAELLE TRRLLDDTAR 130 140 150 160 170 180 DRARAEIDIK RLWEENEELK NKLDKKTKEC TTAEGNVRMY ESRANELNNK YNQANADRKK 190 200 210 220 230 240 LNEDLNEALK ELERLRKQFE ETRKNLEQET LSRVDLENTI QSLREELSFK DQIHSQEINE 250 260 270 280 290 300 SRRIKQTEYS EIDGRLSSEY DAKLKQSLQE LRAQYEEQMQ INRDEIQSLY EDKIQRLQEA 310 320 330 340 350 360 AARTSNSTHK SIEELRSTRV RIDALNANIN ELEQANADLN ARIRDLERQL DNDRERHGQE 370 380 390 400 410 420 IDLLEKELIR LREEMTQQLK EYQDLMDIKV SLDLEIAAYD KLLVGEEARL NITPATNTAT 430 440 450 460 470 480 VQSFSQSLRN STRATPSRRT PSAAVKRKRA VVDESEDHSV ADYYVSASAK GNVEIKEIDP 490 500 510 520 530 540 EGKFVRLFNK GSEEVAIGGW QLQRLINEKG PSTTYKFHRS VRIEPNGVIT VWSADTKASH 550 560 570 580 590 600 EPPSSLVMKS QKWVSADNTR TILLNSEGEA VANLDRIKRI VSQHTSSSRL SRRRSVTAVD 610 620 GNEQLYHQQG DPQQSNEKCA IM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene." Gruenbaum Y., Landesman Y., Drees B., Bare J.W., Saumweber H., Paddy M.R., Sedat J.W., Smith D.E., Benton B.M., Fisher P.A. J. Cell Biol. 106:585-596(1988) [PubMed: 3126192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, POST-TRANSLATIONAL MODIFICATION. Tissue: Embryo.
[2]	Stuurman N., Maus N., Fisher P.A. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 24-39 AND 254-263.
[3]	"Molecular analysis of the Drosophila nuclear lamin gene." Osman M., Paz M., Landesman Y., Fainsod A., Gruenbaum Y. Genomics 8:217-224(1990) [PubMed: 2123469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[6]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[7]	"Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins." Riemer D., Stuurman N., Berrios M., Hunter C., Fisher P.A., Weber K. J. Cell Sci. 108:3189-3198(1995) [PubMed: 7593280] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[8]	"The lamin B receptor of Drosophila melanogaster." Wagner N., Weber D., Seitz S., Krohne G. J. Cell Sci. 117:2015-2028(2004) [PubMed: 15054108] [Abstract] Cited for: SUBUNIT, INTERACTION WITH LBR.
[9]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-10; THR-12; THR-20; SER-25; SER-34; THR-39; SER-41; SER-42; SER-45; THR-47; SER-235; TYR-249; SER-250; SER-311; THR-413; THR-435; SER-442; SER-455; SER-459; SER-595; THR-597 AND SER-615, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07278 mRNA. Translation: CAA30259.1. X16275 Genomic DNA. Translation: CAA34351.1. AE014134 Genomic DNA. Translation: AAF52262.1. BT001506 mRNA. Translation: AAN71261.1.
PIR	A29965. A37103.
RefSeq	NP_476616.1.
UniGene	Dm.312
3D structure databases
HSSP	HSSP built from PDB template 1GK7 based on UniProtKB P08670.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2663N.
IntAct	P08928. 11 interactions.
Genome annotation databases
Ensembl	FBgn0002525. Drosophila melanogaster. [Contig view]
GeneID	33782.
KEGG	dme:Dmel_CG6944.
NMPDR	fig|7227.3.peg.902.
Organism-specific databases
FlyBase	FBgn0002525. Lam.
Phylogenomic databases
HOGENOM	P08928.
OMA	P08928. INDEYQS.
Gene expression databases
ArrayExpress	P08928.
GermOnline	CG6944. Drosophila melanogaster.
Family and domain databases
InterPro	IPR016044. F. IPR001664. IF. IPR001322. IF_tail_C. IPR018039. Intermediate_filament_CS. [Graphical view]
PANTHER	PTHR23239. IF. 1 hit.
Pfam	PF00038. Filament. 1 hit. PF00932. IF_tail. 1 hit. [Graphical view]
PROSITE	PS00226. IF. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	785227.

Entry information

Entry name

LAM0_DROME

Accession

Primary (citable) accession number: P08928
Secondary accession number(s): Q9VMQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 91 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project