Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P08923

- LTK_MOUSE

UniProt

P08923 - LTK_MOUSE

Protein

Leukocyte tyrosine kinase receptor

Gene

Ltk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Orphan receptor with a tyrosine-protein kinase activity. The exact function of this protein is not known. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway. Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei540 – 5401ATPPROSITE-ProRule annotation
    Active sitei639 – 6391Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi512 – 5209ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase activity Source: UniProtKB
    3. transmembrane receptor protein tyrosine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell proliferation Source: UniProtKB
    2. cellular response to retinoic acid Source: Ensembl
    3. negative regulation of apoptotic process Source: UniProtKB
    4. phosphatidylinositol 3-kinase signaling Source: UniProtKB
    5. positive regulation of cardiac muscle cell apoptotic process Source: Ensembl
    6. positive regulation of neuron projection development Source: Ensembl
    7. protein phosphorylation Source: UniProtKB
    8. transmembrane receptor protein tyrosine kinase signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Receptor, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.1. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leukocyte tyrosine kinase receptor (EC:2.7.10.1)
    Gene namesi
    Name:Ltk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:96840. Ltk.

    Subcellular locationi

    Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
    Isoform A : Endoplasmic reticulum
    Note: Retained in the endoplasmic reticulum.
    Isoform B : Endoplasmic reticulum
    Note: Retained in the endoplasmic reticulum.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: UniProtKB
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 888872Leukocyte tyrosine kinase receptorPRO_0000016739Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis
    Modified residuei672 – 6721Phosphotyrosine; by autocatalysisBy similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP08923.
    PRIDEiP08923.

    PTM databases

    PhosphoSiteiP08923.

    Expressioni

    Tissue specificityi

    Subsets of lymphoid and neuronal cells.

    Gene expression databases

    ArrayExpressiP08923.
    BgeeiP08923.
    CleanExiMM_LTK.
    GenevestigatoriP08923.

    Interactioni

    Subunit structurei

    Homodimer when bound to ligand Probable. Part of a complex including LTK, TNK2 and GRB2, in which GRB2 promotes LTK recruitment by TNK2 By similarity. Isoform A binds calnexin.By similarityCurated

    Structurei

    3D structure databases

    ProteinModelPortaliP08923.
    SMRiP08923. Positions 498-789.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini17 – 421405ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini447 – 888442CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei422 – 44625HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini506 – 782277Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117362.
    HOGENOMiHOG000231766.
    HOVERGENiHBG052371.
    InParanoidiA2AQ22.
    KOiK05118.
    OMAiAEGGQGC.
    OrthoDBiEOG7GN2KT.
    TreeFamiTF351636.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR026984. LTK.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR002011. Tyr_kinase_rcpt_2_CS.
    [Graphical view]
    PANTHERiPTHR24416:SF138. PTHR24416:SF138. 1 hit.
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    PRINTSiPR00109. TYRKINASE.
    SMARTiSM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS00239. RECEPTOR_TYR_KIN_II. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative promoter usage and alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform D (identifier: P08923-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGCSHRLLLW LGAAGTILCS NSEFQTPFLT PSLLPVLVLN SQEQKVTPTP    50
    SKLEPASLPN PLGTRGPWVF NTCGASGRSG PTQTQCDGAY TGSSVMVTVG 100
    AAGPLKGVQL WRVPDTGQYL ISAYGAAGGK GAQNHLSRAH GIFLSAVFFL 150
    RRGEPVYILV GQQGQDACPG GSPESQLVCL GESGEHATTY GTERIPGWRR 200
    WAGGGGGGGG ATSIFRLRAG EPEPLLVAAG GGGRSYRRRP DRGRTQAVPE 250
    RLETRAAAPG SGGRGGAAGG GSGWTSRAHS PQAGRSPREG AEGGEGCAEA 300
    WAALRWAAAG GFGGGGGACA AGGGGGGYRG GDTSESDLLW ADGEDGTSFV 350
    HPSGELYLQP LAVTEGHGEV EIRKHPNCSH CPFKDCQWQA ELWTAECTCP 400
    EGTELAVDNV TCMDLPTTAS PLILMGAVVA ALALSLLMMC AVLILVNQKC 450
    QGLWGTRLPG PELELSKLRS SAIRTAPNPY YCQVGLSPAQ PWPLPPGLTE 500
    VSPANVTLLR ALGHGAFGEV YEGLVTGLPG DSSPLPVAIK TLPELCSHQD 550
    ELDFLMEALI ISKFSHQNIV RCVGLSFRSA PRLILLELMS GGDMKSFLRH 600
    SRPHPGQLAP LTMQDLLQLA QDIAQGCHYL EENHFIHRDI AARNCLLSCS 650
    GASRVAKIGD FGMARDIYQA SYYRKGGRTL LPVKWMPPEA LLEGLFTSKT 700
    DSWSFGVLLW EIFSLGYMPY PGHTNQEVLD FIATGNRMDP PRNCPGPVYR 750
    IMTQCWQHQP ELRPDFGSIL ERIQYCTQDP DVLNSPLPVE PGPILEEEEA 800
    SRLGNRSLEG LRSPKPLELS SQNLKSWGGG LLGSWLPSGL KTLKPRCLQP 850
    QNIWNPTYGS WTPRGPQGED TGIEHCNGSS SSSIPGIQ 888

    Note: May be produced by alternative promoter usage.

    Length:888
    Mass (Da):94,471
    Last modified:July 27, 2011 - v3
    Checksum:i7D204A474DEF0AC6
    GO
    Isoform A (identifier: P08923-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-251: Missing.
         252-252: L → M
         271-331: Missing.

    Note: May be produced by alternative promoter usage. Starts at a CUG codon.

    Show »
    Length:576
    Mass (Da):62,749
    Checksum:i3598526F13E50B4E
    GO
    Isoform B (identifier: P08923-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-251: Missing.
         252-252: L → M

    Note: May be produced by alternative promoter usage. Starts at a CUG codon.

    Show »
    Length:637
    Mass (Da):68,323
    Checksum:i72225A1464E47365
    GO
    Isoform C (identifier: P08923-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         271-331: Missing.

    Show »
    Length:827
    Mass (Da):88,897
    Checksum:i906AFE379E266FD6
    GO

    Sequence cautioni

    The sequence CAA30793.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261T → A in AAA39451. (PubMed:8380920)Curated
    Sequence conflicti113 – 1131V → A in AAA39451. (PubMed:8380920)Curated
    Sequence conflicti789 – 7891V → M in CAA36848. (PubMed:1662793)Curated
    Sequence conflicti789 – 7891V → M in AAA39451. (PubMed:8380920)Curated
    Sequence conflicti875 – 8751H → Q in CAA36848. (PubMed:1662793)Curated
    Sequence conflicti875 – 8751H → Q in AAA39451. (PubMed:8380920)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti746 – 7461G → E.1 Publication

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 251251Missing in isoform A and isoform B. 4 PublicationsVSP_002950Add
    BLAST
    Alternative sequencei252 – 2521L → M in isoform A and isoform B. 4 PublicationsVSP_002951
    Alternative sequencei271 – 33161Missing in isoform A and isoform C. 4 PublicationsVSP_002952Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52621 mRNA. Translation: CAA36848.1.
    M90470 mRNA. Translation: AAA39451.1.
    AL844536 Genomic DNA. Translation: CAM18081.1.
    X07984 mRNA. Translation: CAA30793.1. Different initiation.
    CCDSiCCDS16608.1. [P08923-1]
    CCDS16609.1. [P08923-4]
    CCDS50674.1. [P08923-2]
    PIRiI58378.
    S12792.
    RefSeqiNP_976220.2. NM_203345.2. [P08923-1]
    NP_996824.1. NM_206941.1. [P08923-3]
    NP_996825.2. NM_206942.2. [P08923-4]
    UniGeneiMm.1740.

    Genome annotation databases

    EnsembliENSMUST00000028759; ENSMUSP00000028759; ENSMUSG00000027297. [P08923-1]
    ENSMUST00000082130; ENSMUSP00000080774; ENSMUSG00000027297. [P08923-4]
    ENSMUST00000182203; ENSMUSP00000138201; ENSMUSG00000027297. [P08923-2]
    GeneIDi17005.
    KEGGimmu:17005.
    UCSCiuc008lug.1. mouse. [P08923-3]
    uc008lui.1. mouse. [P08923-1]

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52621 mRNA. Translation: CAA36848.1 .
    M90470 mRNA. Translation: AAA39451.1 .
    AL844536 Genomic DNA. Translation: CAM18081.1 .
    X07984 mRNA. Translation: CAA30793.1 . Different initiation.
    CCDSi CCDS16608.1. [P08923-1 ]
    CCDS16609.1. [P08923-4 ]
    CCDS50674.1. [P08923-2 ]
    PIRi I58378.
    S12792.
    RefSeqi NP_976220.2. NM_203345.2. [P08923-1 ]
    NP_996824.1. NM_206941.1. [P08923-3 ]
    NP_996825.2. NM_206942.2. [P08923-4 ]
    UniGenei Mm.1740.

    3D structure databases

    ProteinModelPortali P08923.
    SMRi P08923. Positions 498-789.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P08923.

    Proteomic databases

    PaxDbi P08923.
    PRIDEi P08923.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028759 ; ENSMUSP00000028759 ; ENSMUSG00000027297 . [P08923-1 ]
    ENSMUST00000082130 ; ENSMUSP00000080774 ; ENSMUSG00000027297 . [P08923-4 ]
    ENSMUST00000182203 ; ENSMUSP00000138201 ; ENSMUSG00000027297 . [P08923-2 ]
    GeneIDi 17005.
    KEGGi mmu:17005.
    UCSCi uc008lug.1. mouse. [P08923-3 ]
    uc008lui.1. mouse. [P08923-1 ]

    Organism-specific databases

    CTDi 4058.
    MGIi MGI:96840. Ltk.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117362.
    HOGENOMi HOG000231766.
    HOVERGENi HBG052371.
    InParanoidi A2AQ22.
    KOi K05118.
    OMAi AEGGQGC.
    OrthoDBi EOG7GN2KT.
    TreeFami TF351636.

    Enzyme and pathway databases

    BRENDAi 2.7.10.1. 3474.

    Miscellaneous databases

    NextBioi 291120.
    PROi P08923.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P08923.
    Bgeei P08923.
    CleanExi MM_LTK.
    Genevestigatori P08923.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR026984. LTK.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR002011. Tyr_kinase_rcpt_2_CS.
    [Graphical view ]
    PANTHERi PTHR24416:SF138. PTHR24416:SF138. 1 hit.
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    PRINTSi PR00109. TYRKINASE.
    SMARTi SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS00239. RECEPTOR_TYR_KIN_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ltk receptor tyrosine kinase is expressed in pre-B lymphocytes and cerebral neurons and uses a non-AUG translational initiator."
      Bernards A., de la Monte S.
      EMBO J. 9:2279-2287(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
      Strain: BALB/c.
    2. "Alternatively spliced ltk mRNA in neurons predicts a receptor with a larger putative extracellular domain."
      Haase V.H., Snijders A.J., Cooke S.M., Teng M.N., Kaul D., le Beau M.M., Bruns G.A., Bernards A.
      Oncogene 6:2319-2325(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
      Strain: BALB/c.
    3. "Four tissue-specific mouse ltk mRNAs predict tyrosine kinases that differ upstream of their transmembrane segment."
      Snijders A.J., Haase V.H., Bernards A.
      Oncogene 8:27-35(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C AND D).
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "Leukocytes express a novel gene encoding a putative transmembrane protein-kinase devoid of an extracellular domain."
      Ben-Neriah Y., Bauskin A.R.
      Nature 333:672-676(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 260-888 (ISOFORM A).
    6. "A lymphocyte-specific Ltk tyrosine kinase isoform is retained in the endoplasmic reticulum in association with calnexin."
      Snijders A.J., Ho S.C., Haase V.H., Pillai S., Bernards A.
      J. Biol. Chem. 272:1297-1301(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CALNEXIN (ISOFORM A).
    7. "Gain-of-function polymorphism in mouse and human Ltk: implications for the pathogenesis of systemic lupus erythematosus."
      Li N., Nakamura K., Jiang Y., Tsurui H., Matsuoka S., Abe M., Ohtsuji M., Nishimura H., Kato K., Kawai T., Atsumi T., Koike T., Shirai T., Ueno H., Hirose S.
      Hum. Mol. Genet. 13:171-179(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT GLU-746.
      Strain: NZB.

    Entry informationi

    Entry nameiLTK_MOUSE
    AccessioniPrimary (citable) accession number: P08923
    Secondary accession number(s): A2AQ22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 147 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3