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P08912

- ACM5_HUMAN

UniProt

P08912 - ACM5_HUMAN

Protein

Muscarinic acetylcholine receptor M5

Gene

CHRM5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

    GO - Molecular functioni

    1. G-protein coupled acetylcholine receptor activity Source: ProtInc
    2. phosphatidylinositol phospholipase C activity Source: ProtInc

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
    2. cell proliferation Source: ProtInc
    3. dopamine transport Source: Ensembl
    4. gastric acid secretion Source: InterPro
    5. G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
    6. metabolic process Source: GOC
    7. regulation of phosphatidylinositol dephosphorylation Source: Ensembl
    8. transmission of nerve impulse Source: Ensembl

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_16943. Muscarinic acetylcholine receptors.
    REACT_18283. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muscarinic acetylcholine receptor M5
    Gene namesi
    Name:CHRM5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:1954. CHRM5.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of plasma membrane Source: ProtInc
    3. plasma membrane Source: Reactome
    4. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26486.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 532532Muscarinic acetylcholine receptor M5PRO_0000069042Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi8 – 81N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi103 ↔ 183PROSITE-ProRule annotation
    Modified residuei501 – 5011PhosphothreonineSequence Analysis
    Modified residuei505 – 5051PhosphothreonineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP08912.
    PRIDEiP08912.

    PTM databases

    PhosphoSiteiP08912.

    Expressioni

    Gene expression databases

    ArrayExpressiP08912.
    BgeeiP08912.
    CleanExiHS_CHRM5.
    GenevestigatoriP08912.

    Organism-specific databases

    HPAiHPA013172.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000331108.

    Structurei

    3D structure databases

    ProteinModelPortaliP08912.
    SMRiP08912. Positions 27-222, 439-507.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2929ExtracellularBy similarityAdd
    BLAST
    Topological domaini54 – 6613CytoplasmicBy similarityAdd
    BLAST
    Topological domaini88 – 10417ExtracellularBy similarityAdd
    BLAST
    Topological domaini127 – 14620CytoplasmicBy similarityAdd
    BLAST
    Topological domaini170 – 19122ExtracellularBy similarityAdd
    BLAST
    Topological domaini215 – 443229CytoplasmicBy similarityAdd
    BLAST
    Topological domaini465 – 47814ExtracellularBy similarityAdd
    BLAST
    Topological domaini499 – 53234CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei30 – 5324Helical; Name=1By similarityAdd
    BLAST
    Transmembranei67 – 8721Helical; Name=2By similarityAdd
    BLAST
    Transmembranei105 – 12622Helical; Name=3By similarityAdd
    BLAST
    Transmembranei147 – 16923Helical; Name=4By similarityAdd
    BLAST
    Transmembranei192 – 21423Helical; Name=5By similarityAdd
    BLAST
    Transmembranei444 – 46421Helical; Name=6By similarityAdd
    BLAST
    Transmembranei479 – 49820Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250863.
    HOGENOMiHOG000231484.
    HOVERGENiHBG105720.
    InParanoidiP08912.
    KOiK04133.
    OMAiYDTPKYF.
    PhylomeDBiP08912.
    TreeFamiTF320495.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR000502. Musac_Ach_M5_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view]
    PANTHERiPTHR24249:SF58. PTHR24249:SF58. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00542. MUSCRINICM5R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08912-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGDSYHNAT TVNGTPVNHQ PLERHRLWEV ITIAAVTAVV SLITIVGNVL    50
    VMISFKVNSQ LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWALGS 100
    LACDLWLALD YVASNASVMN LLVISFDRYF SITRPLTYRA KRTPKRAGIM 150
    IGLAWLISFI LWAPAILCWQ YLVGKRTVPL DECQIQFLSE PTITFGTAIA 200
    AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVTKAE KRKPAHRALF 250
    RSCLRCPRPT LAQRERNQAS WSSSRRSTST TGKPSQATGP SANWAKAEQL 300
    TTCSSYPSSE DEDKPATDPV LQVVYKSQGK ESPGEEFSAE ETEETFVKAE 350
    TEKSDYDTPN YLLSPAAAHR PKSQKCVAYK FRLVVKADGN QETNNGCHKV 400
    KIMPCPFPVA KEPSTKGLNP NPSHQMTKRK RVVLVKERKA AQTLSAILLA 450
    FIITWTPYNI MVLVSTFCDK CVPVTLWHLG YWLCYVNSTV NPICYALCNR 500
    TFRKTFKMLL LCRWKKKKVE EKLYWQGNSK LP 532
    Length:532
    Mass (Da):60,074
    Last modified:June 1, 1994 - v2
    Checksum:iEE846186F645DD9B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti526 – 5261Q → P1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80333 Genomic DNA. Translation: AAA51569.1.
    AF026263 mRNA. Translation: AAB95158.1.
    AB084282 mRNA. Translation: BAB91222.1.
    AF498919 mRNA. Translation: AAM18942.1.
    AF385591 mRNA. Translation: AAK68116.1.
    CCDSiCCDS10031.1.
    PIRiJT0530.
    RefSeqiNP_036257.1. NM_012125.3.
    XP_005254198.1. XM_005254141.1.
    UniGeneiHs.584747.

    Genome annotation databases

    EnsembliENST00000383263; ENSP00000372750; ENSG00000184984.
    ENST00000557872; ENSP00000453745; ENSG00000184984.
    GeneIDi1133.
    KEGGihsa:1133.
    UCSCiuc001zhk.1. human.

    Polymorphism databases

    DMDMi543761.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80333 Genomic DNA. Translation: AAA51569.1 .
    AF026263 mRNA. Translation: AAB95158.1 .
    AB084282 mRNA. Translation: BAB91222.1 .
    AF498919 mRNA. Translation: AAM18942.1 .
    AF385591 mRNA. Translation: AAK68116.1 .
    CCDSi CCDS10031.1.
    PIRi JT0530.
    RefSeqi NP_036257.1. NM_012125.3.
    XP_005254198.1. XM_005254141.1.
    UniGenei Hs.584747.

    3D structure databases

    ProteinModelPortali P08912.
    SMRi P08912. Positions 27-222, 439-507.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000331108.

    Chemistry

    BindingDBi P08912.
    ChEMBLi CHEMBL2035.
    DrugBanki DB00572. Atropine.
    DB00767. Benzquinamide.
    DB00785. Cryptenamine.
    DB00725. Homatropine Methylbromide.
    DB01403. Methotrimeprazine.
    DB00340. Metixene.
    DB00334. Olanzapine.
    DB00420. Promazine.
    DB01069. Promethazine.
    DB00777. Propiomazine.
    DB00372. Thiethylperazine.
    GuidetoPHARMACOLOGYi 17.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P08912.

    Polymorphism databases

    DMDMi 543761.

    Proteomic databases

    PaxDbi P08912.
    PRIDEi P08912.

    Protocols and materials databases

    DNASUi 1133.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000383263 ; ENSP00000372750 ; ENSG00000184984 .
    ENST00000557872 ; ENSP00000453745 ; ENSG00000184984 .
    GeneIDi 1133.
    KEGGi hsa:1133.
    UCSCi uc001zhk.1. human.

    Organism-specific databases

    CTDi 1133.
    GeneCardsi GC15P034261.
    H-InvDB HIX0012084.
    HGNCi HGNC:1954. CHRM5.
    HPAi HPA013172.
    MIMi 118496. gene.
    neXtProti NX_P08912.
    PharmGKBi PA26486.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG250863.
    HOGENOMi HOG000231484.
    HOVERGENi HBG105720.
    InParanoidi P08912.
    KOi K04133.
    OMAi YDTPKYF.
    PhylomeDBi P08912.
    TreeFami TF320495.

    Enzyme and pathway databases

    Reactomei REACT_16943. Muscarinic acetylcholine receptors.
    REACT_18283. G alpha (q) signalling events.

    Miscellaneous databases

    GeneWikii Muscarinic_acetylcholine_receptor_M5.
    GenomeRNAii 1133.
    NextBioi 4712.
    PROi P08912.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P08912.
    Bgeei P08912.
    CleanExi HS_CHRM5.
    Genevestigatori P08912.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR000502. Musac_Ach_M5_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view ]
    PANTHERi PTHR24249:SF58. PTHR24249:SF58. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00542. MUSCRINICM5R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes."
      Bonner T.I., Young A.C., Brann M.R., Buckley N.J.
      Neuron 1:403-410(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Rae J.L., Shepard A.R.
      Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lens epithelium.
    3. "Human muscarinic acetylcholine receptor 5 is localized on 15q13.1 and its expression in SH-SY5Y cells."
      Wang H., Yeomans J.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Cloning cholinergic receptors in human keratinocytes."
      Arredondo J., Grando S.A.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-526.
      Tissue: Keratinocyte.

    Entry informationi

    Entry nameiACM5_HUMAN
    AccessioniPrimary (citable) accession number: P08912
    Secondary accession number(s): Q96RG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 149 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    3. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3