P08912 (ACM5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Muscarinic acetylcholine receptor M5

Gene names

Name:

CHRM5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell proliferation Traceable author statement. Source: ProtInc inhibition of adenylate cyclase activity by G-protein coupled acetylcholine receptor signaling pathway Traceable author statement. Source: ProtInc
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement. Source: ProtInc postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled acetylcholine receptor activity Traceable author statement. Source: ProtInc phosphatidylinositol phospholipase C activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Muscarinic acetylcholine receptor M5		PRO_0000069042
Regions
	Topological domain	1 – 29	29	Extracellular By similarity
	Transmembrane	30 – 53	24	Helical; Name=1; By similarity
	Topological domain	54 – 66	13	Cytoplasmic By similarity
	Transmembrane	67 – 87	21	Helical; Name=2; By similarity
	Topological domain	88 – 104	17	Extracellular By similarity
	Transmembrane	105 – 126	22	Helical; Name=3; By similarity
	Topological domain	127 – 146	20	Cytoplasmic By similarity
	Transmembrane	147 – 169	23	Helical; Name=4; By similarity
	Topological domain	170 – 191	22	Extracellular By similarity
	Transmembrane	192 – 214	23	Helical; Name=5; By similarity
	Topological domain	215 – 443	229	Cytoplasmic By similarity
	Transmembrane	444 – 464	21	Helical; Name=6; By similarity
	Topological domain	465 – 478	14	Extracellular By similarity
	Transmembrane	479 – 498	20	Helical; Name=7; By similarity
	Topological domain	499 – 532	34	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	501	1	Phosphothreonine Potential
	Modified residue	505	1	Phosphothreonine Potential
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Disulfide bond	103 ↔ 183		By similarity
Experimental info
	Sequence conflict	526	1	Q → P Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P08912 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: EE846186F645DD9B	FASTA	532	60,074
	10 20 30 40 50 60 MEGDSYHNAT TVNGTPVNHQ PLERHRLWEV ITIAAVTAVV SLITIVGNVL VMISFKVNSQ 70 80 90 100 110 120 LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWALGS LACDLWLALD YVASNASVMN 130 140 150 160 170 180 LLVISFDRYF SITRPLTYRA KRTPKRAGIM IGLAWLISFI LWAPAILCWQ YLVGKRTVPL 190 200 210 220 230 240 DECQIQFLSE PTITFGTAIA AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVTKAE 250 260 270 280 290 300 KRKPAHRALF RSCLRCPRPT LAQRERNQAS WSSSRRSTST TGKPSQATGP SANWAKAEQL 310 320 330 340 350 360 TTCSSYPSSE DEDKPATDPV LQVVYKSQGK ESPGEEFSAE ETEETFVKAE TEKSDYDTPN 370 380 390 400 410 420 YLLSPAAAHR PKSQKCVAYK FRLVVKADGN QETNNGCHKV KIMPCPFPVA KEPSTKGLNP 430 440 450 460 470 480 NPSHQMTKRK RVVLVKERKA AQTLSAILLA FIITWTPYNI MVLVSTFCDK CVPVTLWHLG 490 500 510 520 530 YWLCYVNSTV NPICYALCNR TFRKTFKMLL LCRWKKKKVE EKLYWQGNSK LP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes." Bonner T.I., Young A.C., Brann M.R., Buckley N.J. Neuron 1:403-410(1988) [PubMed: 3272174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Rae J.L., Shepard A.R. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lens epithelium.
[3]	"Human muscarinic acetylcholine receptor 5 is localized on 15q13.1 and its expression in SH-SY5Y cells." Wang H., Yeomans J. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Cloning cholinergic receptors in human keratinocytes." Arredondo J., Grando S.A. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-526. Tissue: Keratinocyte.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80333 Genomic DNA. Translation: AAA51569.1. AF026263 mRNA. Translation: AAB95158.1. AB084282 mRNA. Translation: BAB91222.1. AF498919 mRNA. Translation: AAM18942.1. AF385591 mRNA. Translation: AAK68116.1.
IPI	IPI00031710.
PIR	JT0530.
RefSeq	NP_036257.1. NM_012125.3.
UniGene	Hs.584747.
3D structure databases
ProteinModelPortal	P08912.
SMR	P08912. Positions 31-511.
ModBase	Search...
Protein-protein interaction databases
STRING	P08912.
Protein family/group databases
GPCRDB	Search...
Polymorphism databases
DMDM	543761.
Proteomic databases
PRIDE	P08912.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000327937; ENSP00000331108; ENSG00000184984. ENST00000383263; ENSP00000372750; ENSG00000184984.
GeneID	1133.
KEGG	hsa:1133.
UCSC	uc001zhk.1. human.
Organism-specific databases
CTD	1133.
GeneCards	GC15P034261.
H-InvDB	HIX0012084.
HGNC	HGNC:1954. CHRM5.
HPA	HPA013172.
MIM	118496. gene.
neXtProt	NX_P08912.
PharmGKB	PA26486.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12672.
HOGENOM	HBG713567.
HOVERGEN	HBG105720.
InParanoid	P08912.
OMA	RKPAHRA.
OrthoDB	EOG4H19VH.
PhylomeDB	P08912.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P08912.
Bgee	P08912.
CleanEx	HS_CHRM5.
Genevestigator	P08912.
GermOnline	ENSG00000184984. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000502. Musac_M5_rcpt. IPR000995. Musac_rcpt. [Graphical view]
KO	K04133.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00542. MUSCRINICM5R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00572. Atropine. DB00767. Benzquinamide. DB00785. Cryptenamine. DB00725. Homatropine Methylbromide. DB01403. Methotrimeprazine. DB00340. Metixene. DB00334. Olanzapine. DB00420. Promazine. DB01069. Promethazine. DB00777. Propiomazine. DB00372. Thiethylperazine.
NextBio	4712.
SOURCE	Search...

Entry information

Entry name

ACM5_HUMAN

Accession

Primary (citable) accession number: P08912
Secondary accession number(s): Q96RG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.