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P08912 (ACM5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M5
Gene names
Name:CHRM5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 532532Muscarinic acetylcholine receptor M5
PRO_0000069042

Regions

Topological domain1 – 2929Extracellular By similarity
Transmembrane30 – 5324Helical; Name=1; By similarity
Topological domain54 – 6613Cytoplasmic By similarity
Transmembrane67 – 8721Helical; Name=2; By similarity
Topological domain88 – 10417Extracellular By similarity
Transmembrane105 – 12622Helical; Name=3; By similarity
Topological domain127 – 14620Cytoplasmic By similarity
Transmembrane147 – 16923Helical; Name=4; By similarity
Topological domain170 – 19122Extracellular By similarity
Transmembrane192 – 21423Helical; Name=5; By similarity
Topological domain215 – 443229Cytoplasmic By similarity
Transmembrane444 – 46421Helical; Name=6; By similarity
Topological domain465 – 47814Extracellular By similarity
Transmembrane479 – 49820Helical; Name=7; By similarity
Topological domain499 – 53234Cytoplasmic By similarity

Amino acid modifications

Modified residue5011Phosphothreonine Potential
Modified residue5051Phosphothreonine Potential
Glycosylation81N-linked (GlcNAc...) Potential
Disulfide bond103 ↔ 183 By similarity

Experimental info

Sequence conflict5261Q → P Ref.5

Sequences

Sequence LengthMass (Da)Tools
P08912 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: EE846186F645DD9B

FASTA53260,074
        10         20         30         40         50         60 
MEGDSYHNAT TVNGTPVNHQ PLERHRLWEV ITIAAVTAVV SLITIVGNVL VMISFKVNSQ 

        70         80         90        100        110        120 
LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWALGS LACDLWLALD YVASNASVMN 

       130        140        150        160        170        180 
LLVISFDRYF SITRPLTYRA KRTPKRAGIM IGLAWLISFI LWAPAILCWQ YLVGKRTVPL 

       190        200        210        220        230        240 
DECQIQFLSE PTITFGTAIA AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVTKAE 

       250        260        270        280        290        300 
KRKPAHRALF RSCLRCPRPT LAQRERNQAS WSSSRRSTST TGKPSQATGP SANWAKAEQL 

       310        320        330        340        350        360 
TTCSSYPSSE DEDKPATDPV LQVVYKSQGK ESPGEEFSAE ETEETFVKAE TEKSDYDTPN 

       370        380        390        400        410        420 
YLLSPAAAHR PKSQKCVAYK FRLVVKADGN QETNNGCHKV KIMPCPFPVA KEPSTKGLNP 

       430        440        450        460        470        480 
NPSHQMTKRK RVVLVKERKA AQTLSAILLA FIITWTPYNI MVLVSTFCDK CVPVTLWHLG 

       490        500        510        520        530 
YWLCYVNSTV NPICYALCNR TFRKTFKMLL LCRWKKKKVE EKLYWQGNSK LP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes."
Bonner T.I., Young A.C., Brann M.R., Buckley N.J.
Neuron 1:403-410(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Rae J.L., Shepard A.R.
Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lens epithelium.
[3]"Human muscarinic acetylcholine receptor 5 is localized on 15q13.1 and its expression in SH-SY5Y cells."
Wang H., Yeomans J.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[4]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Cloning cholinergic receptors in human keratinocytes."
Arredondo J., Grando S.A.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-526.
Tissue: Keratinocyte.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80333 Genomic DNA. Translation: AAA51569.1.
AF026263 mRNA. Translation: AAB95158.1.
AB084282 mRNA. Translation: BAB91222.1.
AF498919 mRNA. Translation: AAM18942.1.
AF385591 mRNA. Translation: AAK68116.1.
PIRJT0530.
RefSeqNP_036257.1. NM_012125.3.
XP_005254198.1. XM_005254141.1.
UniGeneHs.584747.

3D structure databases

ProteinModelPortalP08912.
SMRP08912. Positions 27-511.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000331108.

Chemistry

BindingDBP08912.
ChEMBLCHEMBL2094109.
DrugBankDB00572. Atropine.
DB00767. Benzquinamide.
DB00785. Cryptenamine.
DB00725. Homatropine Methylbromide.
DB01403. Methotrimeprazine.
DB00340. Metixene.
DB00334. Olanzapine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB00372. Thiethylperazine.
GuidetoPHARMACOLOGY17.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP08912.

Polymorphism databases

DMDM543761.

Proteomic databases

PaxDbP08912.
PRIDEP08912.

Protocols and materials databases

DNASU1133.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000383263; ENSP00000372750; ENSG00000184984.
ENST00000557872; ENSP00000453745; ENSG00000184984.
GeneID1133.
KEGGhsa:1133.
UCSCuc001zhk.1. human.

Organism-specific databases

CTD1133.
GeneCardsGC15P034261.
H-InvDBHIX0012084.
HGNCHGNC:1954. CHRM5.
HPAHPA013172.
MIM118496. gene.
neXtProtNX_P08912.
PharmGKBPA26486.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG250863.
HOGENOMHOG000231484.
HOVERGENHBG105720.
InParanoidP08912.
KOK04133.
OMAQKYFLSP.
PhylomeDBP08912.
TreeFamTF320495.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP08912.
BgeeP08912.
CleanExHS_CHRM5.
GenevestigatorP08912.

Family and domain databases

InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000502. Musac_Ach_M5_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERPTHR24249:SF109. PTHR24249:SF109. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00542. MUSCRINICM5R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMuscarinic_acetylcholine_receptor_M5.
GenomeRNAi1133.
NextBio4712.
PROP08912.
SOURCESearch...

Entry information

Entry nameACM5_HUMAN
AccessionPrimary (citable) accession number: P08912
Secondary accession number(s): Q96RG7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: June 1, 1994
Last modified: March 19, 2014
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries