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Protein

Muscarinic acetylcholine receptor M5

Gene

CHRM5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

GO - Molecular functioni

  1. G-protein coupled acetylcholine receptor activity Source: ProtInc
  2. phosphatidylinositol phospholipase C activity Source: ProtInc

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
  2. cell proliferation Source: ProtInc
  3. dopamine transport Source: Ensembl
  4. gastric acid secretion Source: InterPro
  5. G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
  6. metabolic process Source: GOC
  7. phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  8. regulation of phosphatidylinositol dephosphorylation Source: Ensembl
  9. sensory perception of chemical stimulus Source: GO_Central
  10. synaptic transmission, cholinergic Source: GO_Central
  11. transmission of nerve impulse Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_16943. Muscarinic acetylcholine receptors.
REACT_18283. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M5
Gene namesi
Name:CHRM5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:1954. CHRM5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2929ExtracellularBy similarityAdd
BLAST
Transmembranei30 – 5324Helical; Name=1By similarityAdd
BLAST
Topological domaini54 – 6613CytoplasmicBy similarityAdd
BLAST
Transmembranei67 – 8721Helical; Name=2By similarityAdd
BLAST
Topological domaini88 – 10417ExtracellularBy similarityAdd
BLAST
Transmembranei105 – 12622Helical; Name=3By similarityAdd
BLAST
Topological domaini127 – 14620CytoplasmicBy similarityAdd
BLAST
Transmembranei147 – 16923Helical; Name=4By similarityAdd
BLAST
Topological domaini170 – 19122ExtracellularBy similarityAdd
BLAST
Transmembranei192 – 21423Helical; Name=5By similarityAdd
BLAST
Topological domaini215 – 443229CytoplasmicBy similarityAdd
BLAST
Transmembranei444 – 46421Helical; Name=6By similarityAdd
BLAST
Topological domaini465 – 47814ExtracellularBy similarityAdd
BLAST
Transmembranei479 – 49820Helical; Name=7By similarityAdd
BLAST
Topological domaini499 – 53234CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. integral component of plasma membrane Source: ProtInc
  3. plasma membrane Source: Reactome
  4. postsynaptic membrane Source: UniProtKB-SubCell
  5. synapse Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26486.

Chemistry

DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00835. Brompheniramine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00363. Clozapine.
DB00785. Cryptenamine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB01142. Doxepin.
DB06702. Fesoterodine.
DB00725. Homatropine Methylbromide.
DB00458. Imipramine.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB01403. Methotrimeprazine.
DB00340. Metixene.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB00715. Paroxetine.
DB00454. Pethidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB01036. Tolterodine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00246. Ziprasidone.

Polymorphism and mutation databases

BioMutaiCHRM5.
DMDMi543761.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 532532Muscarinic acetylcholine receptor M5PRO_0000069042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi8 – 81N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi103 ↔ 183PROSITE-ProRule annotation
Modified residuei501 – 5011PhosphothreonineSequence Analysis
Modified residuei505 – 5051PhosphothreonineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08912.
PRIDEiP08912.

PTM databases

PhosphoSiteiP08912.

Expressioni

Gene expression databases

BgeeiP08912.
CleanExiHS_CHRM5.
ExpressionAtlasiP08912. baseline and differential.
GenevestigatoriP08912.

Organism-specific databases

HPAiHPA013172.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000331108.

Structurei

3D structure databases

ProteinModelPortaliP08912.
SMRiP08912. Positions 27-222, 439-507.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08912.
KOiK04133.
OMAiTQKYFLS.
PhylomeDBiP08912.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000502. Musac_Ach_M5_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF58. PTHR24249:SF58. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00542. MUSCRINICM5R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGDSYHNAT TVNGTPVNHQ PLERHRLWEV ITIAAVTAVV SLITIVGNVL
60 70 80 90 100
VMISFKVNSQ LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWALGS
110 120 130 140 150
LACDLWLALD YVASNASVMN LLVISFDRYF SITRPLTYRA KRTPKRAGIM
160 170 180 190 200
IGLAWLISFI LWAPAILCWQ YLVGKRTVPL DECQIQFLSE PTITFGTAIA
210 220 230 240 250
AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVTKAE KRKPAHRALF
260 270 280 290 300
RSCLRCPRPT LAQRERNQAS WSSSRRSTST TGKPSQATGP SANWAKAEQL
310 320 330 340 350
TTCSSYPSSE DEDKPATDPV LQVVYKSQGK ESPGEEFSAE ETEETFVKAE
360 370 380 390 400
TEKSDYDTPN YLLSPAAAHR PKSQKCVAYK FRLVVKADGN QETNNGCHKV
410 420 430 440 450
KIMPCPFPVA KEPSTKGLNP NPSHQMTKRK RVVLVKERKA AQTLSAILLA
460 470 480 490 500
FIITWTPYNI MVLVSTFCDK CVPVTLWHLG YWLCYVNSTV NPICYALCNR
510 520 530
TFRKTFKMLL LCRWKKKKVE EKLYWQGNSK LP
Length:532
Mass (Da):60,074
Last modified:June 1, 1994 - v2
Checksum:iEE846186F645DD9B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti526 – 5261Q → P (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80333 Genomic DNA. Translation: AAA51569.1.
AF026263 mRNA. Translation: AAB95158.1.
AB084282 mRNA. Translation: BAB91222.1.
AF498919 mRNA. Translation: AAM18942.1.
AF385591 mRNA. Translation: AAK68116.1.
CCDSiCCDS10031.1.
PIRiJT0530.
RefSeqiNP_036257.1. NM_012125.3.
XP_005254198.1. XM_005254141.1.
UniGeneiHs.584747.

Genome annotation databases

EnsembliENST00000383263; ENSP00000372750; ENSG00000184984.
ENST00000557872; ENSP00000453745; ENSG00000184984.
GeneIDi1133.
KEGGihsa:1133.
UCSCiuc001zhk.1. human.

Polymorphism and mutation databases

BioMutaiCHRM5.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80333 Genomic DNA. Translation: AAA51569.1.
AF026263 mRNA. Translation: AAB95158.1.
AB084282 mRNA. Translation: BAB91222.1.
AF498919 mRNA. Translation: AAM18942.1.
AF385591 mRNA. Translation: AAK68116.1.
CCDSiCCDS10031.1.
PIRiJT0530.
RefSeqiNP_036257.1. NM_012125.3.
XP_005254198.1. XM_005254141.1.
UniGeneiHs.584747.

3D structure databases

ProteinModelPortaliP08912.
SMRiP08912. Positions 27-222, 439-507.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000331108.

Chemistry

BindingDBiP08912.
ChEMBLiCHEMBL2094109.
DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00835. Brompheniramine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00363. Clozapine.
DB00785. Cryptenamine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB01142. Doxepin.
DB06702. Fesoterodine.
DB00725. Homatropine Methylbromide.
DB00458. Imipramine.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB01403. Methotrimeprazine.
DB00340. Metixene.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB00715. Paroxetine.
DB00454. Pethidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB01036. Tolterodine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi17.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP08912.

Polymorphism and mutation databases

BioMutaiCHRM5.
DMDMi543761.

Proteomic databases

PaxDbiP08912.
PRIDEiP08912.

Protocols and materials databases

DNASUi1133.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000383263; ENSP00000372750; ENSG00000184984.
ENST00000557872; ENSP00000453745; ENSG00000184984.
GeneIDi1133.
KEGGihsa:1133.
UCSCiuc001zhk.1. human.

Organism-specific databases

CTDi1133.
GeneCardsiGC15P034261.
H-InvDBHIX0012084.
HGNCiHGNC:1954. CHRM5.
HPAiHPA013172.
MIMi118496. gene.
neXtProtiNX_P08912.
PharmGKBiPA26486.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08912.
KOiK04133.
OMAiTQKYFLS.
PhylomeDBiP08912.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiREACT_16943. Muscarinic acetylcholine receptors.
REACT_18283. G alpha (q) signalling events.

Miscellaneous databases

GeneWikiiMuscarinic_acetylcholine_receptor_M5.
GenomeRNAii1133.
NextBioi4712.
PROiP08912.
SOURCEiSearch...

Gene expression databases

BgeeiP08912.
CleanExiHS_CHRM5.
ExpressionAtlasiP08912. baseline and differential.
GenevestigatoriP08912.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000502. Musac_Ach_M5_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF58. PTHR24249:SF58. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00542. MUSCRINICM5R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes."
    Bonner T.I., Young A.C., Brann M.R., Buckley N.J.
    Neuron 1:403-410(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Rae J.L., Shepard A.R.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lens epithelium.
  3. "Human muscarinic acetylcholine receptor 5 is localized on 15q13.1 and its expression in SH-SY5Y cells."
    Wang H., Yeomans J.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Cloning cholinergic receptors in human keratinocytes."
    Arredondo J., Grando S.A.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-526.
    Tissue: Keratinocyte.

Entry informationi

Entry nameiACM5_HUMAN
AccessioniPrimary (citable) accession number: P08912
Secondary accession number(s): Q96RG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: June 1, 1994
Last modified: April 29, 2015
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.