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Protein

Muscarinic acetylcholine receptor M5

Gene

Chrm5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091AntagonistBy similarity

GO - Molecular functioni

  • G-protein coupled acetylcholine receptor activity Source: RGD

GO - Biological processi

  • dopamine transport Source: Ensembl
  • gastric acid secretion Source: InterPro
  • G-protein coupled acetylcholine receptor signaling pathway Source: GOC
  • regulation of phosphatidylinositol dephosphorylation Source: RGD
  • transmission of nerve impulse Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-390648. Muscarinic acetylcholine receptors.
R-RNO-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M5
Gene namesi
Name:Chrm5
Synonyms:Chrm-5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi620027. Chrm5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828ExtracellularBy similarityAdd
BLAST
Transmembranei29 – 5224Helical; Name=1By similarityAdd
BLAST
Topological domaini53 – 6513CytoplasmicBy similarityAdd
BLAST
Transmembranei66 – 8621Helical; Name=2By similarityAdd
BLAST
Topological domaini87 – 10317ExtracellularBy similarityAdd
BLAST
Transmembranei104 – 12522Helical; Name=3By similarityAdd
BLAST
Topological domaini126 – 14520CytoplasmicBy similarityAdd
BLAST
Transmembranei146 – 16823Helical; Name=4By similarityAdd
BLAST
Topological domaini169 – 19022ExtracellularBy similarityAdd
BLAST
Transmembranei191 – 21323Helical; Name=5By similarityAdd
BLAST
Topological domaini214 – 442229CytoplasmicBy similarityAdd
BLAST
Transmembranei443 – 46321Helical; Name=6By similarityAdd
BLAST
Topological domaini464 – 47714ExtracellularBy similarityAdd
BLAST
Transmembranei478 – 49720Helical; Name=7By similarityAdd
BLAST
Topological domaini498 – 53134CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1907609.
GuidetoPHARMACOLOGYi17.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531Muscarinic acetylcholine receptor M5PRO_0000069046Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi7 – 71N-linked (GlcNAc...)Sequence analysis
Disulfide bondi102 ↔ 182PROSITE-ProRule annotation
Modified residuei500 – 5001PhosphothreonineSequence analysis
Modified residuei504 – 5041PhosphothreonineSequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08911.
PRIDEiP08911.

PTM databases

iPTMnetiP08911.
PhosphoSiteiP08911.

Expressioni

Gene expression databases

GenevisibleiP08911. RN.

Interactioni

Protein-protein interaction databases

IntActiP08911. 3 interactions.
MINTiMINT-8038781.
STRINGi10116.ENSRNOP00000008387.

Chemistry

BindingDBiP08911.

Structurei

3D structure databases

ProteinModelPortaliP08911.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08911.
KOiK04133.
OMAiLTSCFSC.
OrthoDBiEOG7V49Z7.
PhylomeDBiP08911.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000502. Musac_Ach_M5_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF58. PTHR24249:SF58. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00542. MUSCRINICM5R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08911-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGESYNEST VNGTPVNHQA LERHGLWEVI TIAVVTAVVS LMTIVGNVLV
60 70 80 90 100
MISFKVNSQL KTVNNYYLLS LACADLIIGI FSMNLYTTYI LMGRWVLGSL
110 120 130 140 150
ACDLWLALDY VASNASVMNL LVISFDRYFS ITRPLTYRAK RTPKRAGIMI
160 170 180 190 200
GLAWLVSFIL WAPAILCWQY LVGKRTVPPD ECQIQFLSEP TITFGTAIAA
210 220 230 240 250
FYIPVSVMTI LYCRIYRETE KRTKDLADLQ GSDSVAEAKK REPAQRTLLR
260 270 280 290 300
SFFSCPRPSL AQRERNQASW SSSRRSTSTT GKTTQATDLS ADWEKAEQVT
310 320 330 340 350
TCSSYPSSED EAKPTTDPVF QMVYKSEAKE SPGKESNTQE TKETVVNTRT
360 370 380 390 400
ENSDYDTPKY FLSPAAAHRL KSQKCVAYKF RLVVKADGTQ ETNNGCRKVK
410 420 430 440 450
IMPCSFPVSK DPSTKGPDPN LSHQMTKRKR MVLVKERKAA QTLSAILLAF
460 470 480 490 500
IITWTPYNIM VLVSTFCDKC VPVTLWHLGY WLCYVNSTIN PICYALCNRT
510 520 530
FRKTFKLLLL CRWKKKKVEE KLYWQGNSKL P
Length:531
Mass (Da):60,137
Last modified:November 1, 1988 - v1
Checksum:i647CE0D5D75A2BB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22926 mRNA. Translation: AAA40658.1.
M22925 Genomic DNA. Translation: AAA41572.1.
PIRiJT0531.
RefSeqiNP_059058.1. NM_017362.4.
UniGeneiRn.44293.

Genome annotation databases

EnsembliENSRNOT00000008387; ENSRNOP00000008387; ENSRNOG00000006397.
GeneIDi53949.
KEGGirno:53949.
UCSCiRGD:620027. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22926 mRNA. Translation: AAA40658.1.
M22925 Genomic DNA. Translation: AAA41572.1.
PIRiJT0531.
RefSeqiNP_059058.1. NM_017362.4.
UniGeneiRn.44293.

3D structure databases

ProteinModelPortaliP08911.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08911. 3 interactions.
MINTiMINT-8038781.
STRINGi10116.ENSRNOP00000008387.

Chemistry

BindingDBiP08911.
ChEMBLiCHEMBL1907609.
GuidetoPHARMACOLOGYi17.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP08911.
PhosphoSiteiP08911.

Proteomic databases

PaxDbiP08911.
PRIDEiP08911.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008387; ENSRNOP00000008387; ENSRNOG00000006397.
GeneIDi53949.
KEGGirno:53949.
UCSCiRGD:620027. rat.

Organism-specific databases

CTDi1133.
RGDi620027. Chrm5.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08911.
KOiK04133.
OMAiLTSCFSC.
OrthoDBiEOG7V49Z7.
PhylomeDBiP08911.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiR-RNO-390648. Muscarinic acetylcholine receptors.
R-RNO-416476. G alpha (q) signalling events.

Miscellaneous databases

PROiP08911.

Gene expression databases

GenevisibleiP08911. RN.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000502. Musac_Ach_M5_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF58. PTHR24249:SF58. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00542. MUSCRINICM5R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes."
    Bonner T.I., Young A.C., Brann M.R., Buckley N.J.
    Neuron 1:403-410(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Molecular cloning and expression of a fifth muscarinic acetylcholine receptor."
    Liao C.-F., Themmen A.P.N., Joho R., Barberis C., Birnbaumer M., Birnbaumer L.
    J. Biol. Chem. 264:7328-7337(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "Muscarinic acetylcholine receptors. Peptide sequencing identifies residues involved in antagonist binding and disulfide bond formation."
    Kurtenbach E., Curtis C.A.M., Pedder E.K., Aitken A., Harris A.C.M., Hulme E.C.
    J. Biol. Chem. 265:13702-13708(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 66-128.

Entry informationi

Entry nameiACM5_RAT
AccessioniPrimary (citable) accession number: P08911
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: June 8, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.