Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P08909

- 5HT2C_RAT

UniProt

P08909 - 5HT2C_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

5-hydroxytryptamine receptor 2C

Gene

Htr2c

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and feeding behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.1 Publication

GO - Molecular functioni

  1. Gq/11-coupled serotonin receptor activity Source: UniProtKB
  2. serotonin receptor activity Source: MGI

GO - Biological processi

  1. behavioral fear response Source: UniProtKB
  2. behavioral response to nicotine Source: RGD
  3. feeding behavior Source: RGD
  4. G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: RGD
  5. inositol phosphate-mediated signaling Source: MGI
  6. locomotory behavior Source: InterPro
  7. negative regulation of dopamine metabolic process Source: RGD
  8. negative regulation of locomotion Source: RGD
  9. organ regeneration Source: RGD
  10. phospholipase C-activating serotonin receptor signaling pathway Source: RGD
  11. phospholipase D-activating G-protein coupled receptor signaling pathway Source: RGD
  12. positive regulation of acetylcholine secretion, neurotransmission Source: RGD
  13. positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: UniProtKB
  14. positive regulation of gamma-aminobutyric acid secretion Source: RGD
  15. positive regulation of vasoconstriction Source: RGD
  16. regulation of appetite Source: UniProtKB
  17. regulation of corticotropin-releasing hormone secretion Source: UniProtKB
  18. regulation of neurological system process Source: UniProtKB
  19. regulation of sensory perception of pain Source: RGD
  20. response to drug Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 2C
Short name:
5-HT-2C
Short name:
5-HT2C
Short name:
5-HTR2C
Alternative name(s):
5-hydroxytryptamine receptor 1C
Short name:
5-HT-1C
Short name:
5-HT1C
Serotonin receptor 2C
Gene namesi
Name:Htr2c
Synonyms:5ht1c, Htr1c
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2848. Htr2c.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 5321ExtracellularBy similarityAdd
BLAST
Transmembranei54 – 7926Helical; Name=1By similarityAdd
BLAST
Topological domaini80 – 9011CytoplasmicBy similarityAdd
BLAST
Transmembranei91 – 11121Helical; Name=2By similarityAdd
BLAST
Topological domaini112 – 12817ExtracellularBy similarityAdd
BLAST
Transmembranei129 – 15123Helical; Name=3By similarityAdd
BLAST
Topological domaini152 – 17120CytoplasmicBy similarityAdd
BLAST
Transmembranei172 – 19423Helical; Name=4By similarityAdd
BLAST
Topological domaini195 – 21420ExtracellularBy similarityAdd
BLAST
Transmembranei215 – 23622Helical; Name=5By similarityAdd
BLAST
Topological domaini237 – 31377CytoplasmicBy similarityAdd
BLAST
Transmembranei314 – 33522Helical; Name=6By similarityAdd
BLAST
Topological domaini336 – 35015ExtracellularBy similarityAdd
BLAST
Transmembranei351 – 37323Helical; Name=7By similarityAdd
BLAST
Topological domaini374 – 46087CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: RGD
  2. external side of plasma membrane Source: MGI
  3. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi458 – 4581S → A or D: Loss of interaction with MPDZ. 1 Publication
Mutagenesisi459 – 4591S → D: No effect on interaction with MPDZ. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232By similarityAdd
BLAST
Chaini33 – 4604285-hydroxytryptamine receptor 2CPRO_0000068961Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)By similarity
Disulfide bondi128 ↔ 208PROSITE-ProRule annotation
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi339 ↔ 343PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

PTM databases

PhosphoSiteiP08909.

Expressioni

Gene expression databases

GenevestigatoriP08909.

Interactioni

Subunit structurei

Interacts with MPDZ. Interacts with ARRB2 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-443850.
STRINGi10116.ENSRNOP00000062375.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MHONMR-B452-460[»]
ProteinModelPortaliP08909.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1406Agonist bindingBy similarity
Regioni326 – 3305Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi152 – 1543DRY motif; important for ligand-induced conformation changesBy similarity
Motifi366 – 3705NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity
Motifi458 – 4603PDZ-binding

Domaini

The PDZ domain-binding motif is involved in the interaction with MPDZ.

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG247243.
HOGENOMiHOG000240378.
HOVERGENiHBG107487.
InParanoidiP08909.
KOiK04157.
PhylomeDBiP08909.

Family and domain databases

Gene3Di1.20.1070.10. 2 hits.
InterProiIPR000377. 5HT2C_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF32. PTHR24247:SF32. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00517. 5HT2CRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08909-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNLGNAVRS LLMHLIGLLV WQFDISISPV AAIVTDTFNS SDGGRLFQFP
60 70 80 90 100
DGVQNWPALS IVVIIIMTIG GNILVIMAVS MEKKLHNATN YFLMSLAIAD
110 120 130 140 150
MLVGLLVMPL SLLAILYDYV WPLPRYLCPV WISLDVLFST ASIMHLCAIS
160 170 180 190 200
LDRYVAIRNP IEHSRFNSRT KAIMKIAIVW AISIGVSVPI PVIGLRDESK
210 220 230 240 250
VFVNNTTCVL NDPNFVLIGS FVAFFIPLTI MVITYFLTIY VLRRQTLMLL
260 270 280 290 300
RGHTEEELAN MSLNFLNCCC KKNGGEEENA PNPNPDQKPR RKKKEKRPRG
310 320 330 340 350
TMQAINNEKK ASKVLGIVFF VFLIMWCPFF ITNILSVLCG KACNQKLMEK
360 370 380 390 400
LLNVFVWIGY VCSGINPLVY TLFNKIYRRA FSKYLRCDYK PDKKPPVRQI
410 420 430 440 450
PRVAATALSG RELNVNIYRH TNERVARKAN DPEPGIEMQV ENLELPVNPS
460
NVVSERISSV
Length:460
Mass (Da):51,917
Last modified:November 1, 1988 - v1
Checksum:iD44E977D8F80047E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21410 Genomic DNA. Translation: AAA42177.1.
PIRiA32605.
RefSeqiNP_036897.2. NM_012765.3.
UniGeneiRn.9935.

Genome annotation databases

GeneIDi25187.
KEGGirno:25187.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21410 Genomic DNA. Translation: AAA42177.1 .
PIRi A32605.
RefSeqi NP_036897.2. NM_012765.3.
UniGenei Rn.9935.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2MHO NMR - B 452-460 [» ]
ProteinModelPortali P08909.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-443850.
STRINGi 10116.ENSRNOP00000062375.

Chemistry

BindingDBi P08909.
ChEMBLi CHEMBL2094123.
DrugBanki DB00540. Nortriptyline.
DB00726. Trimipramine.
GuidetoPHARMACOLOGYi 8.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P08909.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25187.
KEGGi rno:25187.

Organism-specific databases

CTDi 3358.
RGDi 2848. Htr2c.

Phylogenomic databases

eggNOGi NOG247243.
HOGENOMi HOG000240378.
HOVERGENi HBG107487.
InParanoidi P08909.
KOi K04157.
PhylomeDBi P08909.

Miscellaneous databases

NextBioi 605689.

Gene expression databases

Genevestigatori P08909.

Family and domain databases

Gene3Di 1.20.1070.10. 2 hits.
InterProi IPR000377. 5HT2C_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
PANTHERi PTHR24247:SF32. PTHR24247:SF32. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00517. 5HT2CRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of a functional cDNA encoding the serotonin 1c receptor."
    Julius D., McDermott A.B., Axel R., Jessell T.M.
    Science 241:558-564(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Agonist-induced phosphorylation of the serotonin 5-HT2C receptor regulates its interaction with multiple PDZ protein 1."
    Parker L.L., Backstrom J.R., Sanders-Bush E., Shieh B.H.
    J. Biol. Chem. 278:21576-21583(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPDZ, MUTAGENESIS OF SER-458 AND SER-459.

Entry informationi

Entry namei5HT2C_RAT
AccessioniPrimary (citable) accession number: P08909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3