P08909 (5HT2C_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 2C
Short name=5-HT-2C
Short name=5-HT2C
Short name=5-HTR2C

Alternative name(s):
5-hydroxytryptamine receptor 1C
Short name=5-HT-1C
Short name=5-HT1C
Serotonin receptor 2C

Gene names

Name:	Htr2c
Synonyms:	5ht1c, Htr1c

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subunit structure	Interacts with MPDZ. Interacts with ARRB2 By similarity. Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein.
Domain	The PDZ domain-binding motif is involved in the interaction with MPDZ.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to cyclic nucleotide second messenger Traceable author statement. Source: RGD activation of phospholipase C activity by serotonin receptor signaling pathway Inferred from direct assay. Source: RGD activation of phospholipase D activity by G-protein coupled receptor protein signaling pathway Inferred from mutant phenotype. Source: RGD behavioral response to nicotine Inferred from mutant phenotype. Source: RGD feeding behavior Inferred from mutant phenotype. Source: RGD negative regulation of dopamine metabolic process Inferred from mutant phenotype. Source: RGD negative regulation of locomotion Inferred from mutant phenotype. Source: RGD organ regeneration Inferred from expression pattern. Source: RGD positive regulation of acetylcholine secretion Inferred from mutant phenotype. Source: RGD positive regulation of gamma-aminobutyric acid secretion Inferred from mutant phenotype. Source: RGD positive regulation of vasoconstriction Inferred from mutant phenotype. Source: RGD regulation of sensory perception of pain Inferred from mutant phenotype. Source: RGD response to drug Inferred from mutant phenotype. Source: RGD
Cellular component	external side of plasma membrane Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: RGD serotonin receptor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	5-hydroxytryptamine receptor 2C		PRO_0000068961
Regions
	Topological domain	1 – 53	53	Extracellular By similarity
	Transmembrane	54 – 79	26	Helical; Name=1; By similarity
	Topological domain	80 – 90	11	Cytoplasmic By similarity
	Transmembrane	91 – 111	21	Helical; Name=2; By similarity
	Topological domain	112 – 128	17	Extracellular By similarity
	Transmembrane	129 – 151	23	Helical; Name=3; By similarity
	Topological domain	152 – 171	20	Cytoplasmic By similarity
	Transmembrane	172 – 194	23	Helical; Name=4; By similarity
	Topological domain	195 – 214	20	Extracellular By similarity
	Transmembrane	215 – 236	22	Helical; Name=5; By similarity
	Topological domain	237 – 313	77	Cytoplasmic By similarity
	Transmembrane	314 – 335	22	Helical; Name=6; By similarity
	Topological domain	336 – 350	15	Extracellular By similarity
	Transmembrane	351 – 373	23	Helical; Name=7; By similarity
	Topological domain	374 – 460	87	Cytoplasmic By similarity
	Motif	458 – 460	3	PDZ-binding
Amino acid modifications
	Glycosylation	39	1	N-linked (GlcNAc...) By similarity
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	205	1	N-linked (GlcNAc...) Potential
	Disulfide bond	128 ↔ 208		By similarity
Experimental info
	Mutagenesis	458	1	S → A or D: Loss of interaction with MPDZ. Ref.2
	Mutagenesis	459	1	S → D: No effect on interaction with MPDZ. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08909 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: D44E977D8F80047E	FASTA	460	51,917
	10 20 30 40 50 60 MVNLGNAVRS LLMHLIGLLV WQFDISISPV AAIVTDTFNS SDGGRLFQFP DGVQNWPALS 70 80 90 100 110 120 IVVIIIMTIG GNILVIMAVS MEKKLHNATN YFLMSLAIAD MLVGLLVMPL SLLAILYDYV 130 140 150 160 170 180 WPLPRYLCPV WISLDVLFST ASIMHLCAIS LDRYVAIRNP IEHSRFNSRT KAIMKIAIVW 190 200 210 220 230 240 AISIGVSVPI PVIGLRDESK VFVNNTTCVL NDPNFVLIGS FVAFFIPLTI MVITYFLTIY 250 260 270 280 290 300 VLRRQTLMLL RGHTEEELAN MSLNFLNCCC KKNGGEEENA PNPNPDQKPR RKKKEKRPRG 310 320 330 340 350 360 TMQAINNEKK ASKVLGIVFF VFLIMWCPFF ITNILSVLCG KACNQKLMEK LLNVFVWIGY 370 380 390 400 410 420 VCSGINPLVY TLFNKIYRRA FSKYLRCDYK PDKKPPVRQI PRVAATALSG RELNVNIYRH 430 440 450 460 TNERVARKAN DPEPGIEMQV ENLELPVNPS NVVSERISSV

References

[1]	"Molecular characterization of a functional cDNA encoding the serotonin 1c receptor." Julius D., McDermott A.B., Axel R., Jessell T.M. Science 241:558-564(1988) [PubMed: 3399891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Agonist-induced phosphorylation of the serotonin 5-HT2C receptor regulates its interaction with multiple PDZ protein 1." Parker L.L., Backstrom J.R., Sanders-Bush E., Shieh B.H. J. Biol. Chem. 278:21576-21583(2003) [PubMed: 12682077] [Abstract] Cited for: INTERACTION WITH MPDZ, MUTAGENESIS OF SER-458 AND SER-459.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21410 Genomic DNA. Translation: AAA42177.1.
IPI	IPI00187680.
PIR	A32605.
RefSeq	NP_036897.2. NM_012765.3.
UniGene	Rn.9935.
3D structure databases
ProteinModelPortal	P08909.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-443850.
STRING	P08909.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P08909.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25187.
KEGG	rno:25187.
UCSC	U35315. rat.
Organism-specific databases
CTD	3358.
RGD	2848. Htr2c.
Phylogenomic databases
eggNOG	roNOG05307.
GeneTree	ENSGT00580000081527.
HOVERGEN	HBG107487.
InParanoid	P08909.
Gene expression databases
ArrayExpress	P08909.
Genevestigator	P08909.
GermOnline	ENSRNOG00000030877. Rattus norvegicus.
Family and domain databases
InterPro	IPR000377. 5HT2C_rcpt. IPR002231. 5HT_rcpt. IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
KO	K04157.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00517. 5HT2CRECEPTR. PR01101. 5HTRECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	605689.

Entry information

Entry name

5HT2C_RAT

Accession

Primary (citable) accession number: P08909

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	November 16, 2011
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program