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Protein

Collagenase

Gene
N/A
Organism
Hypoderma lineatum (Early cattle grub) (Common cattle grub)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is a serine protease capable of degrading the native triple helix of collagen. Also cleaves the B chain of insulin at the 15-Leu-|-Try-16 and 22-Arg-|-Gly-23 bonds. Hydrolyzes casein, but not Px-Pro-Leu-Gly-Pro-DArg, BzArgNHPh, AcTyrNHPh, 2-naphthyl phosphate, 2-naphthyl butyrate, 2-naphthyl caprylate, 2-naphthyl myristate, L-leucine 2-2-naphthylamide, L-valine 2-naphthylamide, L-cysteine 2-naphthylamide or L-glutarylphenylalanine 2-naphthylamide.2 Publications

Catalytic activityi

Hydrolysis of proteins including native collagen at Xaa-|-Ala bond leaving an N-terminal (75%) and a C-terminal (25%) fragment.2 Publications

Enzyme regulationi

Inhibited by diisopropylfluorophosphate.1 Publication

pH dependencei

Optimum pH is 8.0-8.5. Reversibly inactivated below pH 4.5.1 Publication

Temperature dependencei

Thermostable. No loss of activity occurs after incubation for 2 hours at 60 degrees Celsius. Inactivated after incubation for 2 hours at 75 degrees Celsius, however 45% of activity remains after incubation for 20 minutes at 75 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75Charge relay system1
Active sitei118Charge relay system1
Active sitei210Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Collagen degradation

Enzyme and pathway databases

BRENDAi3.4.21.49. 2751.

Protein family/group databases

MEROPSiS01.121.

Names & Taxonomyi

Protein namesi
Recommended name:
Collagenase (EC:3.4.21.49)
Alternative name(s):
Hypodermin C
Short name:
HC
OrganismiHypoderma lineatum (Early cattle grub) (Common cattle grub)
Taxonomic identifieri7389 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaOestroideaOestridaeHypodermatinaeHypoderma

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000002762217 – 302 PublicationsAdd BLAST14
ChainiPRO_000002762331 – 260CollagenaseAdd BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi60 ↔ 76
Disulfide bondi181 ↔ 196
Disulfide bondi206 ↔ 234

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Developmental stagei

Larval-specific.

Structurei

Secondary structure

1260
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi45 – 52Combined sources8
Beta strandi57 – 66Combined sources10
Beta strandi69 – 72Combined sources4
Helixi74 – 77Combined sources4
Beta strandi80 – 88Combined sources9
Beta strandi96 – 100Combined sources5
Beta strandi102 – 106Combined sources5
Turni112 – 115Combined sources4
Beta strandi120 – 123Combined sources4
Helixi141 – 145Combined sources5
Beta strandi152 – 159Combined sources8
Beta strandi169 – 176Combined sources8
Helixi178 – 182Combined sources5
Beta strandi194 – 197Combined sources4
Beta strandi213 – 216Combined sources4
Turni217 – 220Combined sources4
Beta strandi221 – 228Combined sources8
Beta strandi240 – 244Combined sources5
Helixi245 – 248Combined sources4
Helixi249 – 256Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HYLX-ray1.80A/B31-258[»]
2HLCX-ray1.70A/B31-258[»]
ProteinModelPortaliP08897.
SMRiP08897.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08897.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 257Peptidase S1PROSITE-ProRule annotationAdd BLAST227

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

KOiK20752.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLLVFALA LATTSAFQHP ASIFELREGR IINGYEAYTG LFPYQAGLDI
60 70 80 90 100
TLQDQRRVWC GGSLIDNKWI LTAAHCVHDA VSVVVYLGSA VQYEGEAVVN
110 120 130 140 150
SERIISHSMF NPDTYLNDVA LIKIPHVEYT DNIQPIRLPS GEELNNKFEN
160 170 180 190 200
IWATVSGWGQ SNTDTVILQY TYNLVIDNDR CAQEYPPGII VESTICGDTC
210 220 230 240 250
DGKSPCFGDS GGPFVLSDKN LLIGVVSFVS GAGCESGKPV GFSRVTSYMD
260
WIQQNTGIIF
Length:260
Mass (Da):28,579
Last modified:July 15, 1998 - v3
Checksum:iF8B1AF6350F2D74E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54D → E AA sequence (PubMed:6249306).Curated1
Sequence conflicti56 – 57RR → QD AA sequence (PubMed:6249306).Curated2
Sequence conflicti200C → S in BAB20995 (PubMed:11470181).Curated1
Sequence conflicti200C → S AA sequence (PubMed:3034899).Curated1
Sequence conflicti215 – 216VL → SK AA sequence (PubMed:6303340).Curated2
Sequence conflicti259I → K AA sequence (PubMed:3034899).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74306 mRNA. Translation: CAA52359.1.
AB054066 mRNA. Translation: BAB20995.1.
PIRiA27802.

Genome annotation databases

KEGGiag:CAA52359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74306 mRNA. Translation: CAA52359.1.
AB054066 mRNA. Translation: BAB20995.1.
PIRiA27802.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HYLX-ray1.80A/B31-258[»]
2HLCX-ray1.70A/B31-258[»]
ProteinModelPortaliP08897.
SMRiP08897.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.121.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA52359.

Phylogenomic databases

KOiK20752.

Enzyme and pathway databases

BRENDAi3.4.21.49. 2751.

Miscellaneous databases

EvolutionaryTraceiP08897.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOGS_HYPLI
AccessioniPrimary (citable) accession number: P08897
Secondary accession number(s): Q25083, Q9BPQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.