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P08894 (CHSA_PETHY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chalcone synthase A

EC=2.3.1.74
Alternative name(s):
Naringenin-chalcone synthase A
Gene names
Name:CHSA
OrganismPetunia hybrida (Petunia)
Taxonomic identifier4102 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Tissue specificity

Major expressed member of the gene family in various floral tissues and in seedlings treated with UV light. It is relatively low expressed in tissue culture material.

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
   Biological processFlavonoid biosynthesis
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological_processflavonoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionnaringenin-chalcone synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389Chalcone synthase A
PRO_0000216029

Sites

Active site1641 By similarity

Experimental info

Sequence conflict1281V → F in CAA32731. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P08894 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 0221B8DA1E96EAF6

FASTA38942,525
        10         20         30         40         50         60 
MVTVEEYRKA QRAEGPATVM AIGTATPTNC VDQSTYPDYY FRITNSEHKT DLKEKFKRMC 

        70         80         90        100        110        120 
EKSMIKKRYM HLTEEILKEN PSMCEYMAPS LDARQDIVVV EVPKLGKEAA QKAIKEWGQP 

       130        140        150        160        170        180 
KSKITHLVFC TTSGVDMPGC DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN 

       190        200        210        220        230        240 
NKGARVLVVC SEITAVTFRG PNDTHLDSLV GQALFGDGAG AIIIGSDPIP GVERPLFELV 

       250        260        270        280        290        300 
SAAQTLLPDS HGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLEEAFKPL GISDWNSLFW 

       310        320        330        340        350        360 
IAHPGGPAIL DQVEIKLGLK PEKLKATRNV LSDYGNMSSA CVLFILDEMR KASAKEGLGT 

       370        380 
TGEGLEWGVL FGFGPGLTVE TVVLHSVAT 

« Hide

References

[1]"Floral tissue of Petunia hybrida (V30) expresses only one member of the chalcone synthase multigene family."
Koes R.E., Spelt C.E., Reif H.J., van den Elzen P.J.M., Veltkamp E., Mol J.N.M.
Nucleic Acids Res. 14:5229-5239(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Violet 30.
[2]"Cloning and molecular characterization of the chalcone synthase multigene family of Petunia hybrida."
Koes R.E., Spelt C.E., van den Elzen P.J.M., Mol J.N.M.
Gene 81:245-257(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Violet 30.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04080 mRNA. Translation: CAA27718.1.
X14591 Genomic DNA. Translation: CAA32731.1.
PIRSYPJCN. A23643.
SYPJCA. JS0308.

3D structure databases

ProteinModelPortalP08894.
SMRP08894. Positions 3-388.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHSA_PETHY
AccessionPrimary (citable) accession number: P08894
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways