P08894 (CHSA_PETHY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone synthase A
EC=2.3.1.74

Alternative name(s):
Naringenin-chalcone synthase A

Gene names

Name:

CHSA

Organism

Petunia hybrida (Petunia)

Taxonomic identifier

4102 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Tissue specificity	Major expressed member of the gene family in various floral tissues and in seedlings treated with UV light. It is relatively low expressed in tissue culture material.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Chalcone synthase A		PRO_0000216029
Sites
	Active site	164	1	By similarity
Experimental info
	Sequence conflict	128	1	V → F in CAA32731. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08894 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 0221B8DA1E96EAF6	FASTA	389	42,525
	10 20 30 40 50 60 MVTVEEYRKA QRAEGPATVM AIGTATPTNC VDQSTYPDYY FRITNSEHKT DLKEKFKRMC 70 80 90 100 110 120 EKSMIKKRYM HLTEEILKEN PSMCEYMAPS LDARQDIVVV EVPKLGKEAA QKAIKEWGQP 130 140 150 160 170 180 KSKITHLVFC TTSGVDMPGC DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN 190 200 210 220 230 240 NKGARVLVVC SEITAVTFRG PNDTHLDSLV GQALFGDGAG AIIIGSDPIP GVERPLFELV 250 260 270 280 290 300 SAAQTLLPDS HGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLEEAFKPL GISDWNSLFW 310 320 330 340 350 360 IAHPGGPAIL DQVEIKLGLK PEKLKATRNV LSDYGNMSSA CVLFILDEMR KASAKEGLGT 370 380 TGEGLEWGVL FGFGPGLTVE TVVLHSVAT

References

[1]	"Floral tissue of Petunia hybrida (V30) expresses only one member of the chalcone synthase multigene family." Koes R.E., Spelt C.E., Reif H.J., van den Elzen P.J.M., Veltkamp E., Mol J.N.M. Nucleic Acids Res. 14:5229-5239(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Violet 30.
[2]	"Cloning and molecular characterization of the chalcone synthase multigene family of Petunia hybrida." Koes R.E., Spelt C.E., van den Elzen P.J.M., Mol J.N.M. Gene 81:245-257(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Violet 30. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04080 mRNA. Translation: CAA27718.1. X14591 Genomic DNA. Translation: CAA32731.1.
PIR	SYPJCN. A23643. SYPJCA. JS0308.
3D structure databases
ProteinModelPortal	P08894.
SMR	P08894. Positions 3-388.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00154.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHSA_PETHY

Accession

Primary (citable) accession number: P08894

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program