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Protein

Chalcone synthase A

Gene

CHSA

Organism
Petunia hybrida (Petunia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathway:iflavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase A (EC:2.3.1.74)
Alternative name(s):
Naringenin-chalcone synthase A
Gene namesi
Name:CHSA
OrganismiPetunia hybrida (Petunia)
Taxonomic identifieri4102 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389Chalcone synthase APRO_0000216029Add
BLAST

Expressioni

Tissue specificityi

Major expressed member of the gene family in various floral tissues and in seedlings treated with UV light. It is relatively low expressed in tissue culture material.

Structurei

3D structure databases

ProteinModelPortaliP08894.
SMRiP08894. Positions 3-388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTVEEYRKA QRAEGPATVM AIGTATPTNC VDQSTYPDYY FRITNSEHKT
60 70 80 90 100
DLKEKFKRMC EKSMIKKRYM HLTEEILKEN PSMCEYMAPS LDARQDIVVV
110 120 130 140 150
EVPKLGKEAA QKAIKEWGQP KSKITHLVFC TTSGVDMPGC DYQLTKLLGL
160 170 180 190 200
RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN NKGARVLVVC SEITAVTFRG
210 220 230 240 250
PNDTHLDSLV GQALFGDGAG AIIIGSDPIP GVERPLFELV SAAQTLLPDS
260 270 280 290 300
HGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLEEAFKPL GISDWNSLFW
310 320 330 340 350
IAHPGGPAIL DQVEIKLGLK PEKLKATRNV LSDYGNMSSA CVLFILDEMR
360 370 380
KASAKEGLGT TGEGLEWGVL FGFGPGLTVE TVVLHSVAT
Length:389
Mass (Da):42,525
Last modified:November 1, 1988 - v1
Checksum:i0221B8DA1E96EAF6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281V → F in CAA32731 (PubMed:2806915).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04080 mRNA. Translation: CAA27718.1.
X14591 Genomic DNA. Translation: CAA32731.1.
PIRiA23643. SYPJCN.
JS0308. SYPJCA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04080 mRNA. Translation: CAA27718.1.
X14591 Genomic DNA. Translation: CAA32731.1.
PIRiA23643. SYPJCN.
JS0308. SYPJCA.

3D structure databases

ProteinModelPortaliP08894.
SMRiP08894. Positions 3-388.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00154.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Floral tissue of Petunia hybrida (V30) expresses only one member of the chalcone synthase multigene family."
    Koes R.E., Spelt C.E., Reif H.J., van den Elzen P.J.M., Veltkamp E., Mol J.N.M.
    Nucleic Acids Res. 14:5229-5239(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Violet 30.
  2. "Cloning and molecular characterization of the chalcone synthase multigene family of Petunia hybrida."
    Koes R.E., Spelt C.E., van den Elzen P.J.M., Mol J.N.M.
    Gene 81:245-257(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Violet 30.
    Tissue: Leaf.

Entry informationi

Entry nameiCHSA_PETHY
AccessioniPrimary (citable) accession number: P08894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: July 22, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.