Reviewed, UniProtKB/Swiss-Prot P08893 (PGKE_TRYBB)

Names and origin

Protein names	Recommended name:     Phosphoglycerate kinase, cytosolic     EC=2.7.2.3 Alternative name(s):     Phosphoglycerate kinase B     PGK B allele 4
Organism	Trypanosoma brucei brucei
Taxonomic identifier	5702 [NCBI]
Taxonomic lineage	Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Miscellaneous	In T.brucei, three genes code for phosphoglycerate kinase isozymes, which are transported to different cell compartments.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Phosphoglycerate kinase, cytosolic		PRO_0000145865
Regions
	Nucleotide binding	375 – 378	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	62 – 65	4	Substrate binding By similarity
Sites
	Binding site	39	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	223	1	ATP By similarity
	Binding site	345	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08893-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 98AFF47BEB156D7A	FASTA	420	45,087
	10 20 30 40 50 60 MSLKERKSIN ECDLKGKKVL IRVDFNVPLD DGNITNDYRI RSALPAVQKV LTEGGSCVLM 70 80 90 100 110 120 SHLGRPKGVS MAEGKELRST GGIPGFEQKA TLKPVAKALS ELLSRPVTFA PDCLNAADVV 130 140 150 160 170 180 SKMSPGDVVL LENVRFYKEE GSKSTEEREA MAKILASYGD VYISDAFGTA HRDSATMTGI 190 200 210 220 230 240 PKILGHGAAG YLMEKEISYF AKVLGNPPRP LVAIVGGAKV SDKIQLLDNM LQRIDYLLIG 250 260 270 280 290 300 GAMAYTFLKA QGYSIGISMC EESKLEFARS LLKKAEDRKV QVILPIDHVC HTEFKAVDSP 310 320 330 340 350 360 LITEDQNIPE GHMALDIGPK TIEKYVQTIG KCKSAIWNGP MGVFEMVPYS KGTFAIAKAM 370 380 390 400 410 420 GRGTHEHGLM SIIGGGESAG AAELCGEAAR ISHVSTGGGA SLELLEGKTL PGVTVLDEKE

References

[1]	"Evidence for gene conversion between the phosphoglycerate kinase genes of Trypanosoma brucei." le Blancq S.M., Swinkels B.W., Gibson W.C., Borst P. J. Mol. Biol. 200:439-447(1988) [PubMed: 3267227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X05890 Genomic DNA. Translation: CAA29320.1.
PIR	TVUTG4. S02234.
3D structure databases
HSSP	HSSP built from PDB template 16PK based on UniProtKB P07378.
SMR	P08893. Positions 5-419.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.2.3. 74165.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKE_TRYBB

Accession

Primary (citable) accession number: P08893

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)