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P08893 (PGKE_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase, cytosolic

EC=2.7.2.3
Alternative name(s):
PGK B allele 4
Phosphoglycerate kinase B
OrganismTrypanosoma brucei brucei
Taxonomic identifier5702 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00145.

Miscellaneous

In T.brucei, three genes code for phosphoglycerate kinase isozymes, which are transported to different cell compartments.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420Phosphoglycerate kinase, cytosolic HAMAP-Rule MF_00145
PRO_0000145865

Regions

Nucleotide binding375 – 3784ATP By similarity
Region24 – 263Substrate binding By similarity
Region62 – 654Substrate binding By similarity

Sites

Binding site391Substrate By similarity
Binding site1351Substrate By similarity
Binding site1721Substrate By similarity
Binding site2231ATP By similarity
Binding site3451ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P08893 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 98AFF47BEB156D7A

FASTA42045,087
        10         20         30         40         50         60 
MSLKERKSIN ECDLKGKKVL IRVDFNVPLD DGNITNDYRI RSALPAVQKV LTEGGSCVLM 

        70         80         90        100        110        120 
SHLGRPKGVS MAEGKELRST GGIPGFEQKA TLKPVAKALS ELLSRPVTFA PDCLNAADVV 

       130        140        150        160        170        180 
SKMSPGDVVL LENVRFYKEE GSKSTEEREA MAKILASYGD VYISDAFGTA HRDSATMTGI 

       190        200        210        220        230        240 
PKILGHGAAG YLMEKEISYF AKVLGNPPRP LVAIVGGAKV SDKIQLLDNM LQRIDYLLIG 

       250        260        270        280        290        300 
GAMAYTFLKA QGYSIGISMC EESKLEFARS LLKKAEDRKV QVILPIDHVC HTEFKAVDSP 

       310        320        330        340        350        360 
LITEDQNIPE GHMALDIGPK TIEKYVQTIG KCKSAIWNGP MGVFEMVPYS KGTFAIAKAM 

       370        380        390        400        410        420 
GRGTHEHGLM SIIGGGESAG AAELCGEAAR ISHVSTGGGA SLELLEGKTL PGVTVLDEKE 

« Hide

References

[1]"Evidence for gene conversion between the phosphoglycerate kinase genes of Trypanosoma brucei."
le Blancq S.M., Swinkels B.W., Gibson W.C., Borst P.
J. Mol. Biol. 200:439-447(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05890 Genomic DNA. Translation: CAA29320.1.
PIRTVUTG4. S02234.

3D structure databases

ProteinModelPortalP08893.
SMRP08893. Positions 5-419.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR027250. Pgk_euglenozoa.
IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PIRSF500126. Pgk_euglenozoa. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGKE_TRYBB
AccessionPrimary (citable) accession number: P08893
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways