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P08884

- GRAE_MOUSE

UniProt

P08884 - GRAE_MOUSE

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Protein

Granzyme E

Gene

Gzme

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme is probably necessary for target cell lysis in cell-mediated immune responses.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay systemBy similarity
Active sitei109 – 1091Charge relay systemBy similarity
Active sitei204 – 2041Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. cytolysis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Cytolysis

Enzyme and pathway databases

ReactomeiREACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

Protein family/group databases

MEROPSiS01.399.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme E (EC:3.4.21.-)
Alternative name(s):
CTL serine protease 2
Cytotoxic cell protease 3
Short name:
CCP3
Cytotoxic serine protease 2
D12
MCSP2
Gene namesi
Name:Gzme
Synonyms:Ccp3, Ctla-6, Ctla6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:109265. Gzme.

Subcellular locationi

Cytoplasmic granule
Note: Cytoplasmic granules of cytolytic T-lymphocytes.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Propeptidei19 – 2021 PublicationPRO_0000027407
Chaini21 – 248228Granzyme EPRO_0000027408Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi102 – 1021N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi143 ↔ 210PROSITE-ProRule annotation
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi175 ↔ 189PROSITE-ProRule annotation
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP08884.
PRIDEiP08884.

Expressioni

Gene expression databases

BgeeiP08884.
CleanExiMM_GZME.
GenevestigatoriP08884.

Interactioni

Protein-protein interaction databases

IntActiP08884. 1 interaction.
MINTiMINT-4096698.
STRINGi10090.ENSMUSP00000015588.

Structurei

3D structure databases

ProteinModelPortaliP08884.
SMRiP08884. Positions 21-248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 246226Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP08884.
KOiK01362.
OMAiNSECFIL.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP08884.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08884-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPVLILLTL LLPLGAGAEE IIGGHVVKPH SRPYMAFVKS VDIEGNRRYC
60 70 80 90 100
GGFLVQDDFV LTAAHCRNRT MTVTLGAHNI KAKEETQQII PVAKAIPHPD
110 120 130 140 150
YNATAFFSDI MLLKLESKAK RTKAVRPLKL PRPNARVKPG DVCSVAGWGS
160 170 180 190 200
RSINDTKASA RLREAQLVIQ EDEECKKRFR HYTETTEICA GDLKKIKTPF
210 220 230 240
KGDSGGPLVC DNKAYGLLAY AKNRTISSGV FTKIVHFLPW ISRNMKLL
Length:248
Mass (Da):27,494
Last modified:November 1, 1988 - v1
Checksum:i3A31912A45E93D3F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51L → P in AAB19192. (PubMed:8917549)Curated
Sequence conflicti132 – 1321R → K in AAA37487. (PubMed:3053963)Curated
Sequence conflicti132 – 1321R → K in CAA32255. (PubMed:3053963)Curated
Sequence conflicti150 – 1501S → P(PubMed:8917549)Curated
Sequence conflicti150 – 1501S → P(PubMed:3260382)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36901 mRNA. Translation: AAA37487.1.
X12821 mRNA. Translation: CAA31308.1.
U66474 Genomic DNA. Translation: AAB19192.1.
X56988 Genomic DNA. Translation: CAA40306.1.
J03256 mRNA. Translation: AAA37737.1.
X14093 mRNA. Translation: CAA32255.1.
CCDSiCCDS27143.1.
PIRiS01006.
RefSeqiNP_034503.2. NM_010373.3.
UniGeneiMm.14424.

Genome annotation databases

EnsembliENSMUST00000089549; ENSMUSP00000086978; ENSMUSG00000022156.
GeneIDi14942.
KEGGimmu:14942.
UCSCiuc007ubo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36901 mRNA. Translation: AAA37487.1 .
X12821 mRNA. Translation: CAA31308.1 .
U66474 Genomic DNA. Translation: AAB19192.1 .
X56988 Genomic DNA. Translation: CAA40306.1 .
J03256 mRNA. Translation: AAA37737.1 .
X14093 mRNA. Translation: CAA32255.1 .
CCDSi CCDS27143.1.
PIRi S01006.
RefSeqi NP_034503.2. NM_010373.3.
UniGenei Mm.14424.

3D structure databases

ProteinModelPortali P08884.
SMRi P08884. Positions 21-248.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08884. 1 interaction.
MINTi MINT-4096698.
STRINGi 10090.ENSMUSP00000015588.

Protein family/group databases

MEROPSi S01.399.

Proteomic databases

PaxDbi P08884.
PRIDEi P08884.

Protocols and materials databases

DNASUi 14942.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000089549 ; ENSMUSP00000086978 ; ENSMUSG00000022156 .
GeneIDi 14942.
KEGGi mmu:14942.
UCSCi uc007ubo.1. mouse.

Organism-specific databases

CTDi 14942.
MGIi MGI:109265. Gzme.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00760000118895.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P08884.
KOi K01362.
OMAi NSECFIL.
OrthoDBi EOG7RRF7Z.
PhylomeDBi P08884.
TreeFami TF333630.

Enzyme and pathway databases

Reactomei REACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

Miscellaneous databases

NextBioi 287275.
PROi P08884.
SOURCEi Search...

Gene expression databases

Bgeei P08884.
CleanExi MM_GZME.
Genevestigatori P08884.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation of two cDNA sequences which encode cytotoxic cell proteases."
    Bleackley R.C., Duggan B., Ehrman N., Lobe C.G.
    FEBS Lett. 234:153-159(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Prendergast J.A., Pinkoski M., Wolfenden A., Bleackley R.C.
    Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Long-range disruption of gene expression by a selectable marker cassette."
    Pham C.T.N., MacIvor D.M., Hug B.A., Heusel J.W., Ley T.J.
    Proc. Natl. Acad. Sci. U.S.A. 93:13090-13095(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  4. "Identification and sequencing of cDNA clones encoding the granule-associated serine proteases granzymes D, E, and F of cytolytic T lymphocytes."
    Jenne D.E., Rey C., Haefliger J.-A., Qiao B.-Y., Groscurth P., Tschopp J.
    Proc. Natl. Acad. Sci. U.S.A. 85:4814-4818(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-248.
  5. "Isolation and sequence analysis of serine protease cDNAs from mouse cytolytic T lymphocytes."
    Kwon B.S., Kestler D., Lee E., Wakulchik M., Young J.D.-E.
    J. Exp. Med. 168:1839-1854(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-248.
    Tissue: Cytotoxic T-cell.
  6. "A family of serine esterases in lytic granules of cytolytic T lymphocytes."
    Masson D., Tschopp J.
    Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-40.

Entry informationi

Entry nameiGRAE_MOUSE
AccessioniPrimary (citable) accession number: P08884
Secondary accession number(s): P97389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: October 29, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3