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P08882

- GRAC_MOUSE

UniProt

P08882 - GRAC_MOUSE

Protein

Granzyme C

Gene

Gzmc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    This enzyme is probably necessary for target cell lysis in cell-mediated immune responses.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay systemBy similarity
    Active sitei109 – 1091Charge relay systemBy similarity
    Active sitei204 – 2041Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: RefGenome

    GO - Biological processi

    1. cytolysis Source: UniProtKB-KW
    2. granzyme-mediated apoptotic signaling pathway Source: RefGenome
    3. proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Cytolysis

    Enzyme and pathway databases

    ReactomeiREACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

    Protein family/group databases

    MEROPSiS01.137.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Granzyme C (EC:3.4.21.-)
    Alternative name(s):
    B10
    Cytotoxic cell protease 2
    Short name:
    CCP2
    Gene namesi
    Name:Gzmc
    Synonyms:Ctla-5, Ctla5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:109256. Gzmc.

    Subcellular locationi

    Cytoplasmic granule
    Note: Cytoplasmic granules of cytolytic T-lymphocytes.

    GO - Cellular componenti

    1. secretory granule Source: RefGenome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Propeptidei19 – 2021 PublicationPRO_0000027403
    Chaini21 – 248228Granzyme CPRO_0000027404Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
    Disulfide bondi143 ↔ 210PROSITE-ProRule annotation
    Disulfide bondi174 ↔ 189PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP08882.
    PRIDEiP08882.

    Expressioni

    Gene expression databases

    ArrayExpressiP08882.
    BgeeiP08882.
    CleanExiMM_GZMC.
    GenevestigatoriP08882.

    Interactioni

    Protein-protein interaction databases

    IntActiP08882. 1 interaction.
    MINTiMINT-4096670.

    Structurei

    Secondary structure

    1
    248
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi35 – 417
    Beta strandi43 – 453
    Beta strandi47 – 5610
    Beta strandi59 – 624
    Beta strandi69 – 768
    Beta strandi88 – 9710
    Turni103 – 1064
    Beta strandi111 – 1177
    Beta strandi142 – 1498
    Beta strandi162 – 1687
    Helixi171 – 1788
    Turni179 – 1813
    Turni184 – 1863
    Beta strandi187 – 1904
    Turni200 – 2056
    Beta strandi207 – 2104
    Beta strandi213 – 2208
    Beta strandi223 – 2253
    Beta strandi226 – 2338
    Helixi234 – 2363
    Helixi238 – 2458

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3FZZX-ray2.50A/B21-247[»]
    3G01X-ray2.50A/B21-247[»]
    ProteinModelPortaliP08882.
    SMRiP08882. Positions 21-247.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08882.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 246226Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP08882.
    KOiK01362.
    OMAiRRNAHVK.
    OrthoDBiEOG7RRF7Z.
    PhylomeDBiP08882.
    TreeFamiTF333630.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08882-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPVLILLTL LLPLRAGAEE IIGGNEISPH SRPYMAYYEF LKVGGKKMFC    50
    GGFLVRDKFV LTAAHCKGSS MTVTLGAHNI KAKEETQQII PVAKAIPHPD 100
    YNPDDRSNDI MLLKLVRNAK RTRAVRPLNL PRRNAHVKPG DECYVAGWGK 150
    VTPDGEFPKT LHEVKLTVQK DQVCESQFQS SYNRANEICV GDSKIKGASF 200
    EEDSGGPLVC KRAAAGIVSY GQTDGSAPQV FTRVLSFVSW IKKTMKHS 248
    Length:248
    Mass (Da):27,311
    Last modified:November 1, 1988 - v1
    Checksum:iAA6247655EC52289
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 691S → R in AAA37734. (PubMed:3257230)Curated
    Sequence conflicti181 – 1811S → F in AAA37384. (PubMed:3518058)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22527 Genomic DNA. Translation: AAA85454.1.
    X12822 mRNA. Translation: CAA31309.1.
    M18459 mRNA. Translation: AAA37734.1.
    M12301 mRNA. Translation: AAA37384.1.
    CCDSiCCDS27146.1.
    PIRiB28952. PRMSC2.
    RefSeqiNP_034501.2. NM_010371.2.
    UniGeneiMm.14465.

    Genome annotation databases

    EnsembliENSMUST00000015585; ENSMUSP00000015585; ENSMUSG00000079186.
    GeneIDi14940.
    KEGGimmu:14940.
    UCSCiuc007ubu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22527 Genomic DNA. Translation: AAA85454.1 .
    X12822 mRNA. Translation: CAA31309.1 .
    M18459 mRNA. Translation: AAA37734.1 .
    M12301 mRNA. Translation: AAA37384.1 .
    CCDSi CCDS27146.1.
    PIRi B28952. PRMSC2.
    RefSeqi NP_034501.2. NM_010371.2.
    UniGenei Mm.14465.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3FZZ X-ray 2.50 A/B 21-247 [» ]
    3G01 X-ray 2.50 A/B 21-247 [» ]
    ProteinModelPortali P08882.
    SMRi P08882. Positions 21-247.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08882. 1 interaction.
    MINTi MINT-4096670.

    Protein family/group databases

    MEROPSi S01.137.

    Proteomic databases

    PaxDbi P08882.
    PRIDEi P08882.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000015585 ; ENSMUSP00000015585 ; ENSMUSG00000079186 .
    GeneIDi 14940.
    KEGGi mmu:14940.
    UCSCi uc007ubu.2. mouse.

    Organism-specific databases

    CTDi 14940.
    MGIi MGI:109256. Gzmc.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P08882.
    KOi K01362.
    OMAi RRNAHVK.
    OrthoDBi EOG7RRF7Z.
    PhylomeDBi P08882.
    TreeFami TF333630.

    Enzyme and pathway databases

    Reactomei REACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

    Miscellaneous databases

    EvolutionaryTracei P08882.
    NextBioi 287267.
    PROi P08882.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P08882.
    Bgeei P08882.
    CleanExi MM_GZMC.
    Genevestigatori P08882.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of two cDNA sequences which encode cytotoxic cell proteases."
      Bleackley R.C., Duggan B., Ehrman N., Lobe C.G.
      FEBS Lett. 234:153-159(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Organization of two genes encoding cytotoxic T lymphocyte-specific serine proteases CCPI and CCPII."
      Lobe C.G., Upton C., Duggan B., Ehrman N., Letellier M., Bell J., McFadden G., Bleackley R.C.
      Biochemistry 27:6941-6946(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "cDNA cloning of granzyme C, a granule-associated serine protease of cytolytic T lymphocytes."
      Jenne D.E., Rey C., Masson D., Stanley K.K., Herz J., Plaetinck G., Tschopp J.
      J. Immunol. 140:318-323(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Novel serine proteases encoded by two cytotoxic T lymphocyte-specific genes."
      Lobe C.G., Finlay B.B., Paranchych W., Paetkau V.H., Bleackley R.C.
      Science 232:858-861(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 159-248.
    5. "A family of serine esterases in lytic granules of cytolytic T lymphocytes."
      Masson D., Tschopp J.
      Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-40.

    Entry informationi

    Entry nameiGRAC_MOUSE
    AccessioniPrimary (citable) accession number: P08882
    Secondary accession number(s): Q61389
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3