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P08882 (GRAC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Granzyme C
EC=3.4.21.-
Alternative name(s):
B10
Cytotoxic cell protease 2
Short name=CCP2
Gene names
Name:Gzmc
Synonyms:Ctla-5, Ctla5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme is probably necessary for target cell lysis in cell-mediated immune responses.

Subcellular location

Cytoplasmic granule. Note: Cytoplasmic granules of cytolytic T-lymphocytes.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Biological processCytolysis
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcytolysis

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818
Propeptide19 – 202
PRO_0000027403
Chain21 – 248228Granzyme C
PRO_0000027404

Regions

Domain21 – 246226Peptidase S1

Sites

Active site651Charge relay system By similarity
Active site1091Charge relay system By similarity
Active site2041Charge relay system By similarity

Amino acid modifications

Disulfide bond50 ↔ 66 By similarity
Disulfide bond143 ↔ 210 By similarity
Disulfide bond174 ↔ 189 By similarity

Experimental info

Sequence conflict691S → R in AAA37734. Ref.3
Sequence conflict1811S → F in AAA37384. Ref.4

Secondary structure

1 ....................................... 248
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08882 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: AA6247655EC52289

FASTA24827,311
        10         20         30         40         50         60 
MPPVLILLTL LLPLRAGAEE IIGGNEISPH SRPYMAYYEF LKVGGKKMFC GGFLVRDKFV 

        70         80         90        100        110        120 
LTAAHCKGSS MTVTLGAHNI KAKEETQQII PVAKAIPHPD YNPDDRSNDI MLLKLVRNAK 

       130        140        150        160        170        180 
RTRAVRPLNL PRRNAHVKPG DECYVAGWGK VTPDGEFPKT LHEVKLTVQK DQVCESQFQS 

       190        200        210        220        230        240 
SYNRANEICV GDSKIKGASF EEDSGGPLVC KRAAAGIVSY GQTDGSAPQV FTRVLSFVSW 


IKKTMKHS

« Hide

References

[1]"Isolation of two cDNA sequences which encode cytotoxic cell proteases."
Bleackley R.C., Duggan B., Ehrman N., Lobe C.G.
FEBS Lett. 234:153-159(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Organization of two genes encoding cytotoxic T lymphocyte-specific serine proteases CCPI and CCPII."
Lobe C.G., Upton C., Duggan B., Ehrman N., Letellier M., Bell J., McFadden G., Bleackley R.C.
Biochemistry 27:6941-6946(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"cDNA cloning of granzyme C, a granule-associated serine protease of cytolytic T lymphocytes."
Jenne D.E., Rey C., Masson D., Stanley K.K., Herz J., Plaetinck G., Tschopp J.
J. Immunol. 140:318-323(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Novel serine proteases encoded by two cytotoxic T lymphocyte-specific genes."
Lobe C.G., Finlay B.B., Paranchych W., Paetkau V.H., Bleackley R.C.
Science 232:858-861(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 159-248.
[5]"A family of serine esterases in lytic granules of cytolytic T lymphocytes."
Masson D., Tschopp J.
Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-40.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M22527 Genomic DNA. Translation: AAA85454.1.
X12822 mRNA. Translation: CAA31309.1.
M18459 mRNA. Translation: AAA37734.1.
M12301 mRNA. Translation: AAA37384.1.
IPIIPI00323937.
PIRPRMSC2. B28952.
RefSeqNP_034501.2. NM_010371.2.
UniGeneMm.14465.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3FZZX-ray2.50A/B21-247[»]
3G01X-ray2.50A/B21-247[»]
ProteinModelPortalP08882.
SMRP08882. Positions 21-247.
ModBaseSearch...

Protein family/group databases

MEROPSS01.137.

Proteomic databases

PaxDbP08882.
PRIDEP08882.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000015585; ENSMUSP00000015585; ENSMUSG00000079186.
GeneID14940.
KEGGmmu:14940.

Organism-specific databases

CTD14940.
MGIMGI:109256. Gzmc.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP08882.
KOK01362.
OMAPRRNAHV.
OrthoDBEOG47WNQM.

Gene expression databases

ArrayExpressP08882.
BgeeP08882.
CleanExMM_GZMC.
GenevestigatorP08882.
GermOnlineENSMUSG00000015441. Mus musculus.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. Pept_Ser_Cys. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. False negative.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP08882.
NextBio287267.
SOURCESearch...

Entry information

Entry nameGRAC_MOUSE
AccessionPrimary (citable) accession number: P08882
Secondary accession number(s): Q61389
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: April 3, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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