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P08882

- GRAC_MOUSE

UniProt

P08882 - GRAC_MOUSE

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Protein

Granzyme C

Gene

Gzmc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme is probably necessary for target cell lysis in cell-mediated immune responses.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay systemBy similarity
Active sitei109 – 1091Charge relay systemBy similarity
Active sitei204 – 2041Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. cytolysis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Cytolysis

Enzyme and pathway databases

ReactomeiREACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

Protein family/group databases

MEROPSiS01.137.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme C (EC:3.4.21.-)
Alternative name(s):
B10
Cytotoxic cell protease 2
Short name:
CCP2
Gene namesi
Name:Gzmc
Synonyms:Ctla-5, Ctla5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:109256. Gzmc.

Subcellular locationi

Cytoplasmic granule
Note: Cytoplasmic granules of cytolytic T-lymphocytes.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Propeptidei19 – 2021 PublicationPRO_0000027403
Chaini21 – 248228Granzyme CPRO_0000027404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Disulfide bondi143 ↔ 210PROSITE-ProRule annotation
Disulfide bondi174 ↔ 189PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP08882.
PRIDEiP08882.

Expressioni

Gene expression databases

BgeeiP08882.
CleanExiMM_GZMC.
ExpressionAtlasiP08882. baseline and differential.
GenevestigatoriP08882.

Interactioni

Protein-protein interaction databases

IntActiP08882. 1 interaction.
MINTiMINT-4096670.

Structurei

Secondary structure

1
248
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi35 – 417
Beta strandi43 – 453
Beta strandi47 – 5610
Beta strandi59 – 624
Beta strandi69 – 768
Beta strandi88 – 9710
Turni103 – 1064
Beta strandi111 – 1177
Beta strandi142 – 1498
Beta strandi162 – 1687
Helixi171 – 1788
Turni179 – 1813
Turni184 – 1863
Beta strandi187 – 1904
Turni200 – 2056
Beta strandi207 – 2104
Beta strandi213 – 2208
Beta strandi223 – 2253
Beta strandi226 – 2338
Helixi234 – 2363
Helixi238 – 2458

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FZZX-ray2.50A/B21-247[»]
3G01X-ray2.50A/B21-247[»]
ProteinModelPortaliP08882.
SMRiP08882. Positions 21-247.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08882.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 246226Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP08882.
KOiK01362.
OMAiRRNAHVK.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP08882.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08882-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPVLILLTL LLPLRAGAEE IIGGNEISPH SRPYMAYYEF LKVGGKKMFC
60 70 80 90 100
GGFLVRDKFV LTAAHCKGSS MTVTLGAHNI KAKEETQQII PVAKAIPHPD
110 120 130 140 150
YNPDDRSNDI MLLKLVRNAK RTRAVRPLNL PRRNAHVKPG DECYVAGWGK
160 170 180 190 200
VTPDGEFPKT LHEVKLTVQK DQVCESQFQS SYNRANEICV GDSKIKGASF
210 220 230 240
EEDSGGPLVC KRAAAGIVSY GQTDGSAPQV FTRVLSFVSW IKKTMKHS
Length:248
Mass (Da):27,311
Last modified:November 1, 1988 - v1
Checksum:iAA6247655EC52289
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 691S → R in AAA37734. (PubMed:3257230)Curated
Sequence conflicti181 – 1811S → F in AAA37384. (PubMed:3518058)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22527 Genomic DNA. Translation: AAA85454.1.
X12822 mRNA. Translation: CAA31309.1.
M18459 mRNA. Translation: AAA37734.1.
M12301 mRNA. Translation: AAA37384.1.
CCDSiCCDS27146.1.
PIRiB28952. PRMSC2.
RefSeqiNP_034501.2. NM_010371.2.
UniGeneiMm.14465.

Genome annotation databases

EnsembliENSMUST00000015585; ENSMUSP00000015585; ENSMUSG00000079186.
GeneIDi14940.
KEGGimmu:14940.
UCSCiuc007ubu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22527 Genomic DNA. Translation: AAA85454.1 .
X12822 mRNA. Translation: CAA31309.1 .
M18459 mRNA. Translation: AAA37734.1 .
M12301 mRNA. Translation: AAA37384.1 .
CCDSi CCDS27146.1.
PIRi B28952. PRMSC2.
RefSeqi NP_034501.2. NM_010371.2.
UniGenei Mm.14465.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3FZZ X-ray 2.50 A/B 21-247 [» ]
3G01 X-ray 2.50 A/B 21-247 [» ]
ProteinModelPortali P08882.
SMRi P08882. Positions 21-247.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08882. 1 interaction.
MINTi MINT-4096670.

Protein family/group databases

MEROPSi S01.137.

Proteomic databases

PaxDbi P08882.
PRIDEi P08882.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000015585 ; ENSMUSP00000015585 ; ENSMUSG00000079186 .
GeneIDi 14940.
KEGGi mmu:14940.
UCSCi uc007ubu.2. mouse.

Organism-specific databases

CTDi 14940.
MGIi MGI:109256. Gzmc.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P08882.
KOi K01362.
OMAi RRNAHVK.
OrthoDBi EOG7RRF7Z.
PhylomeDBi P08882.
TreeFami TF333630.

Enzyme and pathway databases

Reactomei REACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

Miscellaneous databases

EvolutionaryTracei P08882.
NextBioi 287267.
PROi P08882.
SOURCEi Search...

Gene expression databases

Bgeei P08882.
CleanExi MM_GZMC.
ExpressionAtlasi P08882. baseline and differential.
Genevestigatori P08882.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation of two cDNA sequences which encode cytotoxic cell proteases."
    Bleackley R.C., Duggan B., Ehrman N., Lobe C.G.
    FEBS Lett. 234:153-159(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Organization of two genes encoding cytotoxic T lymphocyte-specific serine proteases CCPI and CCPII."
    Lobe C.G., Upton C., Duggan B., Ehrman N., Letellier M., Bell J., McFadden G., Bleackley R.C.
    Biochemistry 27:6941-6946(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "cDNA cloning of granzyme C, a granule-associated serine protease of cytolytic T lymphocytes."
    Jenne D.E., Rey C., Masson D., Stanley K.K., Herz J., Plaetinck G., Tschopp J.
    J. Immunol. 140:318-323(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Novel serine proteases encoded by two cytotoxic T lymphocyte-specific genes."
    Lobe C.G., Finlay B.B., Paranchych W., Paetkau V.H., Bleackley R.C.
    Science 232:858-861(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 159-248.
  5. "A family of serine esterases in lytic granules of cytolytic T lymphocytes."
    Masson D., Tschopp J.
    Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-40.

Entry informationi

Entry nameiGRAC_MOUSE
AccessioniPrimary (citable) accession number: P08882
Secondary accession number(s): Q61389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: October 29, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3