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Protein

Phosphocarrier protein HPr

Gene

ptsH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the permease (enzymes II/III).
P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbon catabolite repression (CCR), a mechanism that allows bacteria to coordinate and optimize the utilization of available carbon sources. P-Ser-HPr also plays a role in inducer exclusion, in which it probably interacts with several non-PTS permeases and inhibits their transport activity.

Catalytic activityi

Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine.

Enzyme regulationi

Phosphorylation on Ser-46 inhibits the phosphoryl transfer from enzyme I to HPr.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei15Pros-phosphohistidine intermediate; alternatePROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU13900-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphocarrier protein HPr (EC:2.7.11.-)
Alternative name(s):
Histidine-containing protein
Gene namesi
Name:ptsH
Ordered Locus Names:BSU13900
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi46S → A: No phosphorylation by HPrK/P; abolishes the catabolite repressive effects of glucose on gluconate kinase, glucitol dehydrogenase, and the mannitol-specific catabolic enzymes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001078422 – 88Phosphocarrier protein HPrAdd BLAST87

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine1 Publication1
Modified residuei15Tele-phosphohistidine; alternate1 Publication1
Modified residuei46Phosphoserine; by HPrK/PPROSITE-ProRule annotation1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08877.
PRIDEiP08877.

PTM databases

iPTMnetiP08877.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ccpAP251443EBI-1034482,EBI-5247535

Protein-protein interaction databases

IntActiP08877. 5 interactors.
MINTiMINT-8366889.
STRINGi224308.Bsubs1_010100007716.

Structurei

Secondary structure

188
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Beta strandi10 – 12Combined sources3
Helixi16 – 26Combined sources11
Beta strandi29 – 37Combined sources9
Beta strandi40 – 43Combined sources4
Helixi47 – 53Combined sources7
Beta strandi60 – 67Combined sources8
Helixi70 – 83Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JEMNMR-A2-88[»]
1KKLX-ray2.80H/I/J1-88[»]
1KKMX-ray2.80H/I/J1-88[»]
1SPHX-ray2.00A/B1-88[»]
2FEPX-ray2.45S2-88[»]
2HIDNMR-A2-88[»]
2HPRX-ray2.00A2-88[»]
3OQMX-ray2.96D/S2-88[»]
3OQNX-ray3.30D/S2-88[»]
3OQOX-ray2.97D/S2-88[»]
ProteinModelPortaliP08877.
SMRiP08877.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08877.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 88HPrPROSITE-ProRule annotationAdd BLAST87

Sequence similaritiesi

Belongs to the HPr family.Curated
Contains 1 HPr domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105KRI. Bacteria.
COG1925. LUCA.
HOGENOMiHOG000278399.
InParanoidiP08877.
KOiK11189.
OMAiVIRIRID.
PhylomeDBiP08877.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKTFKVTA DSGIHARPAT VLVQTASKYD ADVNLEYNGK TVNLKSIMGV
60 70 80
MSLGIAKGAE ITISASGADE NDALNALEET MKSEGLGE
Length:88
Mass (Da):9,189
Last modified:January 23, 2007 - v3
Checksum:i2B13DBD63DD636A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12832 Genomic DNA. Translation: CAA31317.1.
AL009126 Genomic DNA. Translation: CAB13263.1.
PIRiS04177. WQBSPH.
RefSeqiNP_389273.1. NC_000964.3.
WP_003154654.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13263; CAB13263; BSU13900.
GeneIDi939259.
9779573.
KEGGibsu:BSU13900.
PATRICi18974563. VBIBacSub10457_1473.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12832 Genomic DNA. Translation: CAA31317.1.
AL009126 Genomic DNA. Translation: CAB13263.1.
PIRiS04177. WQBSPH.
RefSeqiNP_389273.1. NC_000964.3.
WP_003154654.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JEMNMR-A2-88[»]
1KKLX-ray2.80H/I/J1-88[»]
1KKMX-ray2.80H/I/J1-88[»]
1SPHX-ray2.00A/B1-88[»]
2FEPX-ray2.45S2-88[»]
2HIDNMR-A2-88[»]
2HPRX-ray2.00A2-88[»]
3OQMX-ray2.96D/S2-88[»]
3OQNX-ray3.30D/S2-88[»]
3OQOX-ray2.97D/S2-88[»]
ProteinModelPortaliP08877.
SMRiP08877.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08877. 5 interactors.
MINTiMINT-8366889.
STRINGi224308.Bsubs1_010100007716.

PTM databases

iPTMnetiP08877.

Proteomic databases

PaxDbiP08877.
PRIDEiP08877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13263; CAB13263; BSU13900.
GeneIDi939259.
9779573.
KEGGibsu:BSU13900.
PATRICi18974563. VBIBacSub10457_1473.

Phylogenomic databases

eggNOGiENOG4105KRI. Bacteria.
COG1925. LUCA.
HOGENOMiHOG000278399.
InParanoidiP08877.
KOiK11189.
OMAiVIRIRID.
PhylomeDBiP08877.

Enzyme and pathway databases

BioCyciBSUB:BSU13900-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP08877.
PROiP08877.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di3.30.1340.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR001020. PTS_HPr_His_P_site.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamiPF00381. PTS-HPr. 1 hit.
[Graphical view]
PRINTSiPR00107. PHOSPHOCPHPR.
SUPFAMiSSF55594. SSF55594. 1 hit.
TIGRFAMsiTIGR01003. PTS_HPr_family. 1 hit.
PROSITEiPS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
PS00589. PTS_HPR_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTHP_BACSU
AccessioniPrimary (citable) accession number: P08877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 167 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.