Reviewed, UniProtKB/Swiss-Prot P08874 (ABRB_BACSU)

Names and origin

Protein names

Recommended name:
    Transition state regulatory protein abrB

Gene names

Name:	abrB
Synonyms:	cpsX
Ordered Locus Names:	BSU00370

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	96 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. It controls the expression of genes spovG and tycA. AbrB binds to the tycA promoter region at two A- and T-rich sites, it may be the sole repressor of tycA transcription. Ref.5
Sequence similarities	To B.subtilis abh and spoVT.
Caution	It is uncertain whether Met-1 or Met-3 is the initiator.

Ontologies

Keywords
Biological process	Sporulation Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 96

96

Transition state regulatory protein abrB

PRO_0000064431

Experimental info

Mutagenesis

56

1

C → Y: Loss of DNA-binding activity. Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

96

Helix Strand Turn

Details...

Beta strand

8 – 11

4

Beta strand

17 – 19

3

Helix

22 – 27

6

Beta strand

35 – 41

7

Beta strand

44 – 49

6

Sequences

	Sequence		Length	Mass (Da)
	P08874-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 6A964557F5590CFE	FASTA	96	10,773
	10 20 30 40 50 60 MFMKSTGIVR KVDELGRVVI PIELRRTLGI AEKDALEIYV DDEKIILKKY KPNMTCQVTG 70 80 90 EVSDDNLKLA GGKLVLSKEG AEQIISEIQN QLQNLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis." Perego M., Spiegelman G.B., Hoch J.A. Mol. Microbiol. 2:689-699(1988) [PubMed: 3145384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"AbrB, a regulator of gene expression in Bacillus, interacts with the transcription initiation regions of a sporulation gene and an antibiotic biosynthesis gene." Robertson J.R., Gocht M., Marahiel M.A., Zuber P. Proc. Natl. Acad. Sci. U.S.A. 86:8457-8461(1989) [PubMed: 2554317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[5]	"The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein." Strauch M.A., Spiegelman G.B., Perego M., Johnson W.C., Burbulys D., Hoch J.A. EMBO J. 8:1615-1621(1989) [PubMed: 2504584] [Abstract] Cited for: FUNCTION.
[6]	"Mutant analysis of interaction of the Bacillus subtilis transcription regulator AbrB with the antibiotic biosynthesis gene tycA." Fuerbass R., Marahiel M.A. FEBS Lett. 287:153-156(1991) [PubMed: 1908787] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-56.
[7]	"Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis." Hamon M.A., Stanley N.R., Britton R.A., Grossman A.D., Lazazzera B.A. Mol. Microbiol. 52:847-860(2004) [PubMed: 15101989] [Abstract] Cited for: ABRB-REGULATED GENES.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X12820 Genomic DNA. Translation: CAA31307.1.
M26917 Genomic DNA. Translation: AAA22195.1. Different initiation.
D26185 Genomic DNA. Translation: BAA05272.1.
X61953 Genomic DNA. Translation: CAA43955.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB11813.1.

PIR

S03096.

RefSeq

NP_387918.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YFB	NMR	-	A/B	2-53	[»]
1YSF	NMR	-	A/B	2-53	[»]
1Z0R	NMR	-	A/B	3-55	[»]
2K1N	NMR	-	A/B/C/D	3-57	[»]
2RO4	NMR	-	A/B	3-55	[»]

ModBase

Search...

Genome annotation databases

GeneID

937009.

GenomeReviews

Gene locus BSU00370 in contig AL009126_GR.

KEGG

bsu:BSU00370.

NMPDR

fig|224308.1.peg.37.

Organism-specific databases

SubtiList

BG10100. abrB. [Micado]

CMR

Search...

Phylogenomic databases

HOGENOM

P08874.

OMA

EVSDDNF

Family and domain databases

InterPro

IPR006339. Tscrpt_reg_AbrB.
IPR007159. Tscrpt_reg_AbrB/SpoVT.
[Graphical view]

Pfam

PF04014. SpoVT_AbrB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01439. lp_hng_hel_AbrB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ABRB_BACSU

Accession

Primary (citable) accession number: P08874

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	November 24, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)