P08874 (ABRB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transition state regulatory protein AbrB

Gene names

Name:	abrB
Synonyms:	cpsX
Ordered Locus Names:	BSU00370

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	96 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. It controls the expression of genes spovG and tycA. AbrB binds to the tycA promoter region at two A- and T-rich sites, it may be the sole repressor of tycA transcription. Ref.5
Sequence similarities	Contains 1 AbrB-like domain. To B.subtilis Abh and SpoVT.
Caution	It is uncertain whether Met-1 or Met-3 is the initiator.
Sequence caution	The sequence AAA22195.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAA43955.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Sporulation Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 96

96

Transition state regulatory protein AbrB

PRO_0000064431

Regions

Domain

10 – 52

43

AbrB-like

Experimental info

Mutagenesis

56

1

C → Y: Loss of DNA-binding activity. Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

96

Helix Strand Turn

Details...

Beta strand

8 – 11

4

Beta strand

17 – 19

3

Helix

22 – 27

6

Beta strand

35 – 41

7

Beta strand

44 – 49

6

Sequences

	Sequence		Length	Mass (Da)
	P08874 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 6A964557F5590CFE	FASTA	96	10,773
	10 20 30 40 50 60 MFMKSTGIVR KVDELGRVVI PIELRRTLGI AEKDALEIYV DDEKIILKKY KPNMTCQVTG 70 80 90 EVSDDNLKLA GGKLVLSKEG AEQIISEIQN QLQNLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis." Perego M., Spiegelman G.B., Hoch J.A. Mol. Microbiol. 2:689-699(1988) [PubMed: 3145384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"AbrB, a regulator of gene expression in Bacillus, interacts with the transcription initiation regions of a sporulation gene and an antibiotic biosynthesis gene." Robertson J.R., Gocht M., Marahiel M.A., Zuber P. Proc. Natl. Acad. Sci. U.S.A. 86:8457-8461(1989) [PubMed: 2554317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[5]	"The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein." Strauch M.A., Spiegelman G.B., Perego M., Johnson W.C., Burbulys D., Hoch J.A. EMBO J. 8:1615-1621(1989) [PubMed: 2504584] [Abstract] Cited for: FUNCTION.
[6]	"Mutant analysis of interaction of the Bacillus subtilis transcription regulator AbrB with the antibiotic biosynthesis gene tycA." Fuerbass R., Marahiel M.A. FEBS Lett. 287:153-156(1991) [PubMed: 1908787] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-56.
[7]	"Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis." Hamon M.A., Stanley N.R., Britton R.A., Grossman A.D., Lazazzera B.A. Mol. Microbiol. 52:847-860(2004) [PubMed: 15101989] [Abstract] Cited for: ABRB-REGULATED GENES.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X12820 Genomic DNA. Translation: CAA31307.1.
M26917 Genomic DNA. Translation: AAA22195.1. Different initiation.
D26185 Genomic DNA. Translation: BAA05272.1.
X61953 Genomic DNA. Translation: CAA43955.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB11813.1.

PIR

S03096.

RefSeq

NP_387918.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YFB	NMR	-	A/B	2-53	[»]
1YSF	NMR	-	A/B	2-53	[»]
1Z0R	NMR	-	A/B	3-55	[»]
2K1N	NMR	-	A/B/C/D	3-57	[»]
2RO4	NMR	-	A/B	3-55	[»]

ProteinModelPortal

P08874.

SMR

P08874. Positions 3-57.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46306N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000003485; EBBACP00000003485; EBBACG00000003478.

GeneID

937009.

GenomeReviews

Gene locus BSU00370 in contig AL009126_GR.

KEGG

bsu:BSU00370.

NMPDR

fig|224308.1.peg.37.

PATRIC

18971545. VBIBacSub10457_0038.

Organism-specific databases

GenoList

BSU00370. [Micado]

Phylogenomic databases

GeneTree

EBGT00070000031935.

HOGENOM

HBG305668.

OMA

GAEQIIN.

PhylomeDB

P08874.

ProtClustDB

CLSK886557.

Enzyme and pathway databases

BioCyc

BSUB:BSU00370-MONOMER.

Family and domain databases

InterPro

IPR006339. Tscrpt_reg_AbrB.
IPR007159. Tscrpt_reg_AbrB/SpoVT.
[Graphical view]

KO

K06284.

Pfam

PF04014. SpoVT_AbrB. 1 hit.
[Graphical view]

SMART

SM00966. SpoVT_AbrB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01439. Lp_hng_hel_AbrB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ABRB_BACSU

Accession

Primary (citable) accession number: P08874

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	January 25, 2012
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program