Reviewed, UniProtKB/Swiss-Prot P08873 (PA20_NOTSC)

Names and origin

Protein names	Recommended name:     Phospholipase A2     EC=3.1.1.4 Alternative name(s):     Notechis 11'2     Phosphatidylcholine 2-acylhydrolase
Organism	Notechis scutatus scutatus (Mainland tiger snake) (Common tiger snake)
Taxonomic identifier	70142 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Notechis

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 27	8	Ref.2		PRO_0000022938
	Chain	28 – 145	118	Phospholipase A2		PRO_0000022939
Sites
	Active site	75	1	By similarity
	Active site	119	1	By similarity
	Metal binding	55	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	57	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	59	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	76	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	38 ↔ 98		By similarity
	Disulfide bond	54 ↔ 144		By similarity
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	71 ↔ 125		By similarity
	Disulfide bond	78 ↔ 118		By similarity
	Disulfide bond	87 ↔ 111		By similarity
	Disulfide bond	105 ↔ 116		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08873-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 3F508029C6E0F001	FASTA	145	15,903
	10 20 30 40 50 60 MYPAHLLVLL TVCVSLLEAS SIPARPLNLY QFGNMIQCAN HGRRPTLAYA DYGCYCGAGG 70 80 90 100 110 120 SGTPVDELDR CCKAHDDCYG EAGKKGCYPT LTLYSWQCIE KTPTCNSKTG CERSVCDCDA 130 140 TAAKCFAKAP YNKKNYNIDT EKRCQ

References

[1]	"Complete amino acid sequence of a PLA2 from the tiger snake Notechis scutatus scutatus as deduced from a complementary DNA." Ducancel F., Guignery Frelat G., Menez A., Boulain J.-C., Bouchier C. Nucleic Acids Res. 16:9049-9049(1988) [PubMed: 3174443] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.
[2]	"Notechis 11'2, a non-toxic phospholipase A2 from the venom of Notechis scutatus scutatus." Bouchier C., Boyot P., Tesson F., Tremeau O., Bouet F., Hodgson D., Boulain J.-C., Menez A. Eur. J. Biochem. 202:493-500(1991) [PubMed: 1761049] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-145. Tissue: Venom.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X12605 mRNA. Translation: CAA31125.1.
PIR	S01390.
3D structure databases
HSSP	HSSP built from PDB template 1AE7 based on UniProtKB P00608.
SMR	P08873. Positions 28-145.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P08873.
Enzyme and pathway databases
BRENDA	3.1.1.4. 292759.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
ProDom	PD000303. PhospholipaseA2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA20_NOTSC

Accession

Primary (citable) accession number: P08873

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)