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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation1 Publication

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Mg2+. Mn2+ can replace Mg2+ as the divalent metal. The role of the metal is to bind 5-phosphoribose 1-diphosphate and form a Mg-5-phosphoribose 1-diphosphate complex which then serves as substrate for OPRTase.1 Publication

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265-phosphoribose 1-diphosphate1 Publication1
Binding sitei995-phosphoribose 1-diphosphate; shared with dimeric partner1 Publication1
Binding sitei1005-phosphoribose 1-diphosphate1 Publication1
Binding sitei1035-phosphoribose 1-diphosphate; shared with dimeric partner1 Publication1
Binding sitei1055-phosphoribose 1-diphosphate; shared with dimeric partner1 Publication1
Binding sitei128Orotate1 Publication1
Binding sitei156Orotate1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

SABIO-RKP08870.
UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrE1 Publication
Ordered Locus Names:STM3733
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19K → Q: No loss of activity. 1 Publication1
Mutagenesisi26K → A or Q: Reduced activity. 1 Publication1
Mutagenesisi73K → A or Q: Significant loss of activity. 1 Publication1
Mutagenesisi100K → A: Reduced activity. 1 Publication1
Mutagenesisi103K → A or Q: Drastically reduced activity. 1 Publication1
Mutagenesisi124D → N: Loss of activity. 1 Publication1
Mutagenesisi125D → N: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001107342 – 213Orotate phosphoribosyltransferaseAdd BLAST212

Proteomic databases

PaxDbiP08870.
PRIDEiP08870.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

STRINGi99287.STM3733.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 14Combined sources12
Beta strandi17 – 24Combined sources8
Beta strandi30 – 35Combined sources6
Helixi37 – 39Combined sources3
Helixi43 – 60Combined sources18
Beta strandi65 – 68Combined sources4
Turni71 – 73Combined sources3
Helixi74 – 89Combined sources16
Beta strandi94 – 98Combined sources5
Beta strandi104 – 106Combined sources3
Beta strandi109 – 114Combined sources6
Beta strandi118 – 123Combined sources6
Beta strandi128 – 130Combined sources3
Helixi132 – 142Combined sources11
Beta strandi146 – 155Combined sources10
Beta strandi161 – 165Combined sources5
Helixi166 – 174Combined sources9
Beta strandi177 – 183Combined sources7
Helixi184 – 193Combined sources10
Helixi195 – 197Combined sources3
Helixi198 – 211Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LH0X-ray2.00A/B1-213[»]
1OPRX-ray2.30A1-213[»]
1STOX-ray2.60A1-213[»]
ProteinModelPortaliP08870.
SMRiP08870.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08870.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 35Orotate binding1 Publication2
Regioni72 – 735-phosphoribose 1-diphosphate binding1 Publication2
Regioni124 – 1325-phosphoribose 1-diphosphate binding1 Publication9

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP2. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037974.
KOiK00762.
OMAiMKAYQRQ.
PhylomeDBiP08870.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPYQRQFIE FALNKQVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLALLG
60 70 80 90 100
RFYAEALVDS GIEFDLLFGP AYKGIPIATT TAVALAEHHD KDLPYCFNRK
110 120 130 140 150
EAKDHGEGGS LVGSALQGRV MLVDDVITAG TAIRESMEII QAHGATLAGV
160 170 180 190 200
LISLDRQERG RGEISAIQEV ERDYGCKVIS IITLKDLIAY LEEKPDMAEH
210
LAAVRAYREE FGV
Length:213
Mass (Da):23,562
Last modified:January 23, 2007 - v3
Checksum:i963A8BE3DD9113B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19547 Genomic DNA. Translation: CAA79607.1.
AE006468 Genomic DNA. Translation: AAL22592.1.
PIRiS32801.
RefSeqiNP_462633.1. NC_003197.1.
WP_000806167.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL22592; AAL22592; STM3733.
GeneIDi1255257.
KEGGistm:STM3733.
PATRICi32386305. VBISalEnt20916_3949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19547 Genomic DNA. Translation: CAA79607.1.
AE006468 Genomic DNA. Translation: AAL22592.1.
PIRiS32801.
RefSeqiNP_462633.1. NC_003197.1.
WP_000806167.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LH0X-ray2.00A/B1-213[»]
1OPRX-ray2.30A1-213[»]
1STOX-ray2.60A1-213[»]
ProteinModelPortaliP08870.
SMRiP08870.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM3733.

Proteomic databases

PaxDbiP08870.
PRIDEiP08870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL22592; AAL22592; STM3733.
GeneIDi1255257.
KEGGistm:STM3733.
PATRICi32386305. VBISalEnt20916_3949.

Phylogenomic databases

eggNOGiENOG4107QP2. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037974.
KOiK00762.
OMAiMKAYQRQ.
PhylomeDBiP08870.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
SABIO-RKP08870.

Miscellaneous databases

EvolutionaryTraceiP08870.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRE_SALTY
AccessioniPrimary (citable) accession number: P08870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.