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Protein

Chymotrypsin-like elastase family member 3B

Gene

CELA3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Efficient protease with alanine specificity but only little elastolytic activity.

Catalytic activityi

Preferential cleavage: Ala-|-Xaa. Does not hydrolyze elastin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73Charge relay systemBy similarity1
Active sitei123Charge relay systemBy similarity1
Active sitei217Charge relay systemBy similarity1

GO - Molecular functioni

  • peptidase activity Source: ProtInc
  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • cholesterol metabolic process Source: ProtInc
  • proteolysis Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciZFISH:HS11406-MONOMER.

Protein family/group databases

MEROPSiS01.205.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-like elastase family member 3B (EC:3.4.21.70)
Alternative name(s):
Elastase IIIB
Elastase-3B
Protease E
Gene namesi
Name:CELA3B
Synonyms:ELA3B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:15945. CELA3B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi23436.
OpenTargetsiENSG00000219073.
PharmGKBiPA27737.

Polymorphism and mutation databases

BioMutaiCELA3B.
DMDMi317373457.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Or 16Sequence analysisAdd BLAST15
PropeptideiPRO_000002769916 – 28Activation peptideSequence analysisAdd BLAST13
ChainiPRO_000002770029 – 270Chymotrypsin-like elastase family member 3BAdd BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi58 ↔ 74PROSITE-ProRule annotation
GlycosylationiCAR_000212114N-linked (GlcNAc...)1 Publication1
Disulfide bondi117 ↔ 120Curated
Disulfide bondi157 ↔ 223PROSITE-ProRule annotation
Disulfide bondi188 ↔ 204PROSITE-ProRule annotation
Disulfide bondi213 ↔ 244PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP08861.
PeptideAtlasiP08861.
PRIDEiP08861.

2D gel databases

SWISS-2DPAGEP08861.

PTM databases

iPTMnetiP08861.
PhosphoSitePlusiP08861.
UniCarbKBiP08861.

Expressioni

Tissue specificityi

Pancreas. Not detectable in keratinocytes.1 Publication

Gene expression databases

BgeeiENSG00000219073.
CleanExiHS_ELA3B.
ExpressionAtlasiP08861. baseline and differential.
GenevisibleiP08861. HS.

Organism-specific databases

HPAiHPA045650.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000338369.

Structurei

3D structure databases

ProteinModelPortaliP08861.
SMRiP08861.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 268Peptidase S1PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
InParanoidiP08861.
KOiK01345.
OMAiDGTWQVH.
OrthoDBiEOG091G0DF7.
PhylomeDBiP08861.
TreeFamiTF330455.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08861-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLRLLSSLL LVAVASGYGP PSSRPSSRVV NGEDAVPYSW PWQVSLQYEK
60 70 80 90 100
SGSFYHTCGG SLIAPDWVVT AGHCISSSRT YQVVLGEYDR AVKEGPEQVI
110 120 130 140 150
PINSGDLFVH PLWNRSCVAC GNDIALIKLS RSAQLGDAVQ LASLPPAGDI
160 170 180 190 200
LPNETPCYIT GWGRLYTNGP LPDKLQEALL PVVDYEHCSR WNWWGSSVKK
210 220 230 240 250
TMVCAGGDIR SGCNGDSGGP LNCPTEDGGW QVHGVTSFVS AFGCNTRRKP
260 270
TVFTRVSAFI DWIEETIASH
Length:270
Mass (Da):29,263
Last modified:January 11, 2011 - v3
Checksum:iF738C5F8F5195D8C
GO

Sequence cautioni

The sequence CAH71872 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAH71873 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4R → G in AAA36482 (PubMed:15489334).Curated1
Sequence conflicti64A → G in AAA36482 (PubMed:15489334).Curated1
Sequence conflicti129 – 131Missing AA sequence (PubMed:2737288).Curated3
Sequence conflicti164R → P in AAA36482 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02544679R → W.3 PublicationsCorresponds to variant rs7528405dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16630 mRNA. Translation: AAA36482.1.
AK315798 mRNA. Translation: BAG38141.1.
AL590556 Genomic DNA. Translation: CAH71871.1.
AL590556 Genomic DNA. Translation: CAH71872.1. Sequence problems.
AL590556 Genomic DNA. Translation: CAH71873.1. Sequence problems.
CH471134 Genomic DNA. Translation: EAW94999.1.
BC005216 mRNA. Translation: AAH05216.1.
M18692 mRNA. Translation: AAA58454.1.
CCDSiCCDS219.1.
PIRiB29934.
RefSeqiNP_031378.1. NM_007352.3.
UniGeneiHs.728752.

Genome annotation databases

EnsembliENST00000337107; ENSP00000338369; ENSG00000219073.
GeneIDi23436.
KEGGihsa:23436.
UCSCiuc001bfk.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16630 mRNA. Translation: AAA36482.1.
AK315798 mRNA. Translation: BAG38141.1.
AL590556 Genomic DNA. Translation: CAH71871.1.
AL590556 Genomic DNA. Translation: CAH71872.1. Sequence problems.
AL590556 Genomic DNA. Translation: CAH71873.1. Sequence problems.
CH471134 Genomic DNA. Translation: EAW94999.1.
BC005216 mRNA. Translation: AAH05216.1.
M18692 mRNA. Translation: AAA58454.1.
CCDSiCCDS219.1.
PIRiB29934.
RefSeqiNP_031378.1. NM_007352.3.
UniGeneiHs.728752.

3D structure databases

ProteinModelPortaliP08861.
SMRiP08861.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000338369.

Protein family/group databases

MEROPSiS01.205.

PTM databases

iPTMnetiP08861.
PhosphoSitePlusiP08861.
UniCarbKBiP08861.

Polymorphism and mutation databases

BioMutaiCELA3B.
DMDMi317373457.

2D gel databases

SWISS-2DPAGEP08861.

Proteomic databases

PaxDbiP08861.
PeptideAtlasiP08861.
PRIDEiP08861.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337107; ENSP00000338369; ENSG00000219073.
GeneIDi23436.
KEGGihsa:23436.
UCSCiuc001bfk.4. human.

Organism-specific databases

CTDi23436.
DisGeNETi23436.
GeneCardsiCELA3B.
HGNCiHGNC:15945. CELA3B.
HPAiHPA045650.
neXtProtiNX_P08861.
OpenTargetsiENSG00000219073.
PharmGKBiPA27737.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
InParanoidiP08861.
KOiK01345.
OMAiDGTWQVH.
OrthoDBiEOG091G0DF7.
PhylomeDBiP08861.
TreeFamiTF330455.

Enzyme and pathway databases

BioCyciZFISH:HS11406-MONOMER.

Miscellaneous databases

GeneWikiiCELA3B.
GenomeRNAii23436.
PROiP08861.

Gene expression databases

BgeeiENSG00000219073.
CleanExiHS_ELA3B.
ExpressionAtlasiP08861. baseline and differential.
GenevisibleiP08861. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEL3B_HUMAN
AccessioniPrimary (citable) accession number: P08861
Secondary accession number(s): B2RE44
, P11423, Q5VU28, Q5VU29, Q5VU30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 11, 2011
Last modified: November 2, 2016
This is version 162 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally thought to be elastase 1.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.