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Protein

Calpastatin

Gene

CAST

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI27.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpastatin
Alternative name(s):
Calpain inhibitor
Cleaved into the following chain:
Gene namesi
Name:CAST
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718CalpastatinPRO_0000004168Add
BLAST
Chaini290 – 718429Erythrocyte calpastatinPRO_0000004169Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki32 – 32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei49 – 491N6-acetyllysineBy similarity
Modified residuei86 – 861PhosphoserineBy similarity
Modified residuei133 – 1331PhosphoserineBy similarity
Modified residuei222 – 2221PhosphoserineBy similarity
Modified residuei243 – 2431PhosphoserineBy similarity
Modified residuei290 – 2901Blocked amino end (Ser); in form erythrocyte
Modified residuei367 – 3671PhosphoserineBy similarity
Modified residuei369 – 3691PhosphoserineBy similarity
Modified residuei376 – 3761PhosphoserineBy similarity
Modified residuei444 – 4441PhosphoserineBy similarity
Modified residuei520 – 5201PhosphoserineBy similarity
Modified residuei531 – 5311PhosphoserineBy similarity
Modified residuei579 – 5791PhosphoserineBy similarity
Modified residuei581 – 5811PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006752.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati170 – 22253Inhibitory domain 1Add
BLAST
Repeati307 – 35953Inhibitory domain 2Add
BLAST
Repeati450 – 50354Inhibitory domain 3Add
BLAST
Repeati587 – 64054Inhibitory domain 4Add
BLAST

Domaini

Each of the four flexible inhibitory domains can inhibit one calcium-bound calpain molecule by occupying both sides of the active site.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFF9. Eukaryota.
ENOG4111YF0. LUCA.
HOGENOMiHOG000082405.
HOVERGENiHBG000183.
InParanoidiP08855.
KOiK04281.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPAEAKAVP ISKEMEGPHP HSKKRHRRQD AKTEPEKSQS TKPPVDHEKK
60 70 80 90 100
AQEGKPKEHT KPKSTHKHAS DGEGKHGRNE KTASRSKEPV TPAKRTEPET
110 120 130 140 150
KPQDTKPAGG KSVAAGTTAA PGKAGDPKKE KKSLPAAALA EPKPDEPSGK
160 170 180 190 200
SGMDAALDDL IDTLGEPSET QEDSTAYTGP EISDPMSSTY IEELGKREVT
210 220 230 240 250
IPPKYRELLE KKTGVAGPPP DSVTPLGPDD AIDALSSDFT CSSPVASGKE
260 270 280 290 300
AGKEAAKSAG EVLEAESAKV MRAAAPPQEK KRKVEEDAMS DQALEALSAS
310 320 330 340 350
LGTRMAEPEL DLSSIKEVAE AKRKEEKVEK CGEDDETVPA EYRLKPATDK
360 370 380 390 400
DGKPLLPEPA EKPKPRSESE LIDELSKDFS QAKSNEKQPK PTGKTEESKA
410 420 430 440 450
AVPAPVAEAV PRTSMCSIQP VPPKPASLQK STVPDDAVEA LAGSLGRKEA
460 470 480 490 500
DPEEGKPVAD KIKEKSKEEE REKLGEKEET IPPDYRLEEA KDKDGKPLLP
510 520 530 540 550
SEPTAQLPAL SEDLLLDALS EDFSGPSSAS SLKFDDAMLS AAVSEVVSQS
560 570 580 590 600
PASITRATAP PPDTRPSNKE LDDALDKLSD SLGQRQPDPD ENKPMEDKVK
610 620 630 640 650
ERAKKEHKDK LGERDDTIPP EYRHLLDQGE QDKPEKPPTK KSKEIKKPAG
660 670 680 690 700
DQDPIDALSG DLDSCPPAAE TSQATEKDKS KTTTASSSKA AKHGDKAKDS
710
AQTTEETSKP KANEKNAS
Length:718
Mass (Da):76,965
Last modified:November 1, 1988 - v1
Checksum:iB57F2B85D386A6BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16476 mRNA. Translation: AAA31186.1.
PIRiA26615.
RefSeqiNP_001075739.1. NM_001082270.1.
UniGeneiOcu.1838.

Genome annotation databases

GeneIDi100009098.
KEGGiocu:100009098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16476 mRNA. Translation: AAA31186.1.
PIRiA26615.
RefSeqiNP_001075739.1. NM_001082270.1.
UniGeneiOcu.1838.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006752.

Protein family/group databases

MEROPSiI27.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009098.
KEGGiocu:100009098.

Organism-specific databases

CTDi831.

Phylogenomic databases

eggNOGiENOG410IFF9. Eukaryota.
ENOG4111YF0. LUCA.
HOGENOMiHOG000082405.
HOVERGENiHBG000183.
InParanoidiP08855.
KOiK04281.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICAL_RABIT
AccessioniPrimary (citable) accession number: P08855
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 11, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In erythrocytes the protein lacks the N-terminal region of the liver inhibitor but retains three inhibitory units.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.