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Protein

Serine carboxypeptidase 2

Gene

CBP2

Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1581
Binding sitei251Substrate1
Active sitei3611
Active sitei4131
Binding sitei414Substrate1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Protein family/group databases

ESTHERiwheat-cbp02. Carboxypeptidase_S10.
MEROPSiS10.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase 2 (EC:3.4.16.6)
Alternative name(s):
CPDW-II
Short name:
CP-WII
Carboxypeptidase D
Serine carboxypeptidase II
Cleaved into the following 2 chains:
Alternative name(s):
Serine carboxypeptidase II chain A
Alternative name(s):
Serine carboxypeptidase II chain B
Gene namesi
Name:CBP2
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unassembled WGS sequence

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000043121 – 259Serine carboxypeptidase 2 chain AAdd BLAST259
PropeptideiPRO_0000274569260 – 286Linker peptideAdd BLAST27
ChainiPRO_0000004313287 – 444Serine carboxypeptidase 2 chain BAdd BLAST158

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi65 ↔ 324Interchain (between A and B chains)
Glycosylationi116N-linked (GlcNAc...)1
Glycosylationi127N-linked (GlcNAc...)1
Disulfide bondi222 ↔ 234
Disulfide bondi258 ↔ 291Interchain (between A and B chains)
Glycosylationi259N-linked (GlcNAc...)1
Glycosylationi312N-linked (GlcNAc...)1
Glycosylationi318N-linked (GlcNAc...)1

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP08819.

Interactioni

Subunit structurei

Carboxypeptidase II is a dimer, where each monomer is composed of two chains linked by a disulfide bond.2 Publications

Structurei

Secondary structure

1444
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni7 – 9Combined sources3
Beta strandi24 – 32Combined sources9
Turni33 – 36Combined sources4
Beta strandi37 – 44Combined sources8
Helixi48 – 50Combined sources3
Beta strandi55 – 59Combined sources5
Turni62 – 64Combined sources3
Turni67 – 70Combined sources4
Helixi71 – 74Combined sources4
Beta strandi75 – 81Combined sources7
Helixi83 – 85Combined sources3
Beta strandi88 – 90Combined sources3
Helixi95 – 97Combined sources3
Beta strandi99 – 104Combined sources6
Beta strandi114 – 117Combined sources4
Helixi118 – 122Combined sources5
Helixi126 – 143Combined sources18
Helixi145 – 147Combined sources3
Beta strandi151 – 158Combined sources8
Helixi160 – 174Combined sources15
Beta strandi180 – 189Combined sources10
Helixi193 – 205Combined sources13
Turni206 – 208Combined sources3
Helixi212 – 222Combined sources11
Beta strandi227 – 229Combined sources3
Helixi232 – 245Combined sources14
Helixi292 – 301Combined sources10
Helixi303 – 308Combined sources6
Beta strandi312 – 315Combined sources4
Helixi326 – 330Combined sources5
Helixi340 – 348Combined sources9
Beta strandi352 – 358Combined sources7
Beta strandi362 – 364Combined sources3
Helixi366 – 374Combined sources9
Beta strandi380 – 389Combined sources10
Beta strandi392 – 400Combined sources9
Beta strandi403 – 408Combined sources6
Helixi415 – 418Combined sources4
Helixi420 – 432Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BCRX-ray2.50A1-263[»]
B285-444[»]
1BCSX-ray2.08A1-263[»]
B285-444[»]
1WHSX-ray2.00A6-260[»]
B287-439[»]
1WHTX-ray2.00A5-260[»]
B287-439[»]
3SC2X-ray2.20A1-259[»]
B287-438[»]
ProteinModelPortaliP08819.
SMRiP08819.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08819.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 53Substrate binding3
Regioni157 – 159Substrate binding3

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08819-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
VEPSGHAADR IARLPGQPAV DFDMYSGYIT VDEGAGRSLF YLLQEAPEDA
60 70 80 90 100
QPAPLVLWLN GGPGCSSVAY GASEELGAFR VKPRGAGLVL NEYRWNKVAN
110 120 130 140 150
VLFLDSPAGV GFSYTNTSSD IYTSGDNRTA HDSYAFLAKW FERFPHYKYR
160 170 180 190 200
DFYIAGESYA GHYVPELSQL VHRSKNPVIN LKGFMVGNGL IDDYHDYVGT
210 220 230 240 250
FEFWWNHGIV SDDTYRRLKE ACLHDSFIHP SPACDAATDV ATAEQGNIDM
260 270 280 290 300
YSLYTPVCNI TSSSSSSSSS LSQQRRSRGR YPWLTGSYDP CTERYSTAYY
310 320 330 340 350
NRRDVQMALH ANVTGAMNYT WATCSDTINT HWHDAPRSML PIYRELIAAG
360 370 380 390 400
LRIWVFSGDT DAVVPLTATR YSIGALGLPT TTSWYPWYDD QEVGGWSQVY
410 420 430 440
KGLTLVSVRG AGHEVPLHRP RQALVLFQYF LQGKPMPGQT KNAT
Length:444
Mass (Da):49,506
Last modified:February 6, 2007 - v2
Checksum:i983B9BCC1C2DECB2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209I → L in CAI64396 (PubMed:2324088).Curated1
Sequence conflicti216R → Q in CAI64396 (PubMed:2324088).Curated1
Sequence conflicti219K → R in CAI64396 (PubMed:2324088).Curated1
Sequence conflicti307M → T in CAI64396 (PubMed:2324088).Curated1
Sequence conflicti322A → S in CAI64396 (PubMed:2324088).Curated1
Sequence conflicti440 – 441TK → AT in CAI64396 (PubMed:2324088).Curated2
Sequence conflicti444T → TVA in CAI64396 (PubMed:2324088).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti1 – 3Missing in a' chain. 3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ890016 mRNA. Translation: CAI64396.1.
PIRiA29639.
UniGeneiTa.56555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ890016 mRNA. Translation: CAI64396.1.
PIRiA29639.
UniGeneiTa.56555.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BCRX-ray2.50A1-263[»]
B285-444[»]
1BCSX-ray2.08A1-263[»]
B285-444[»]
1WHSX-ray2.00A6-260[»]
B287-439[»]
1WHTX-ray2.00A5-260[»]
B287-439[»]
3SC2X-ray2.20A1-259[»]
B287-438[»]
ProteinModelPortaliP08819.
SMRiP08819.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERiwheat-cbp02. Carboxypeptidase_S10.
MEROPSiS10.005.

Proteomic databases

PRIDEiP08819.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP08819.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBP2_WHEAT
AccessioniPrimary (citable) accession number: P08819
Secondary accession number(s): Q4W1G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.