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P08818 (CBP2_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 3, 2012. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine carboxypeptidase 2

EC=3.4.16.6
Alternative name(s):
CP-MII
Carboxypeptidase D
Serine carboxypeptidase II

Cleaved into the following 2 chains:

  1. Serine carboxypeptidase 2 chain A
    Alternative name(s):
    Serine carboxypeptidase II chain A
  2. Serine carboxypeptidase 2 chain B
    Alternative name(s):
    Serine carboxypeptidase II chain B
Gene names
Name:CBP2
Synonyms:CXP;2
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in the degradation of small peptides (2-5 residues) or in the degradation of storage proteins in the embryo.

Catalytic activity

Preferential release of a C-terminal arginine or lysine residue.

Subunit structure

Carboxypeptidase II is a dimer, where each monomer is composed of two chains linked by a disulfide bond.

Subcellular location

Secreted. Note: Secreted into the endosperm.

Developmental stage

Simultaneously present in aleurone and endosperm between 20 and 30 days postanthesis. Accumulates in the developing grain and is stored in its active form in the mature grain. Also found in the roots and shoots of the growing seedling.

Sequence similarities

Belongs to the peptidase S10 family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionCarboxypeptidase
Hydrolase
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type carboxypeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 Ref.2
Chain35 – 294260Serine carboxypeptidase 2 chain A
PRO_0000004309
Propeptide295 – 31319Linker peptide By similarity
PRO_0000004310
Chain314 – 476163Serine carboxypeptidase 2 chain B
PRO_0000004311

Sites

Active site1901 By similarity
Active site3901 By similarity
Active site4431 By similarity

Amino acid modifications

Modified residue3141Blocked amino end (Thr)
Glycosylation1481N-linked (GlcNAc...)
Glycosylation1591N-linked (GlcNAc...)
Glycosylation2911N-linked (GlcNAc...)
Glycosylation3411N-linked (GlcNAc...)
Glycosylation3471N-linked (GlcNAc...)
Glycosylation3521N-linked (GlcNAc...); partial
Glycosylation3521O-linked (GalNAc...); in variant 351-AT-352
Glycosylation4721N-linked (GlcNAc...)
Disulfide bond97 ↔ 353Interchain (between A and B chains) By similarity
Disulfide bond254 ↔ 266 By similarity
Disulfide bond290 ↔ 320Interchain (between A and B chains) By similarity

Natural variations

Natural variant351 – 3522TN → AT.

Experimental info

Sequence conflict1811Y → R AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P08818 [UniParc].

Last modified May 2, 2002. Version 2.
Checksum: 18685725B1A6B5E4

FASTA47652,625
        10         20         30         40         50         60 
MRTTTRRLPP APAAAAVLLA ALTCLLLRPA AVAAAGGHAA DRIVRLPGQP EVDFDMYSGY 

        70         80         90        100        110        120 
ITVDEAAGRS LFYLLQEAPE EAQPAPLVLW LNGGPGCSSV AYGASEELGA FRVMPRGAGL 

       130        140        150        160        170        180 
VLNEYRWNKV ANVLFLDSPA GVGFSYTNTS SDIYTSGDNR TAHDSYAFLA AWFERFPHYK 

       190        200        210        220        230        240 
YREFYVAGES YAGHYVPELS QLVHRSGNPV INLKGFMVGN GLIDDYHDYV GTFEFWWNHG 

       250        260        270        280        290        300 
IVSDDTYRRL KDACLHDSFI HPSPACDAAT DVATAEQGNI DMYSLYTPVC NISSSSSSSS 

       310        320        330        340        350        360 
LSRRRTRGRY PWLTGSYDPC TERYSTAYYN RRDVQTALHA NVTGAMNYTW TNCSDTINTH 

       370        380        390        400        410        420 
WHDAPRSMLP IYRELIAAGL RIWVFSGDTD AVVPLTATRY SIGALGLATT TSWYPWYDDL 

       430        440        450        460        470 
QEVGGWSQVY KGLTLVSVRG AGHEVPLHRP RQALILFQQF LQGKPMPGRT TNVTVA 

« Hide

References

[1]"The gene family of serine carboxypeptidases in barley."
Rocher A., Lok F., Cameron-Mills V., von Wettstein D.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Igri.
Tissue: Etiolated leaf.
[2]"Primary structure of carboxypeptidase II from malted barley."
Soerensen S.B., Svendsen I., Breddam K.
Carlsberg Res. Commun. 52:285-295(1987)
Cited for: PROTEIN SEQUENCE OF 35-294 AND 314-472, GLYCOSYLATION OF VARIANT 351-AT-352.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09602 Genomic DNA. Translation: CAA70815.1.
PIRT05701.

3D structure databases

ProteinModelPortalP08818.
SMRP08818. Positions 37-292, 316-469.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS10.005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP08818.

Gene expression databases

GenevestigatorP08818.

Family and domain databases

InterProIPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERPTHR11802. PTHR11802. 1 hit.
PfamPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSPR00724. CRBOXYPTASEC.
PROSITEPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCBP2_HORVU
AccessionPrimary (citable) accession number: P08818
Secondary accession number(s): P93177
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: May 2, 2002
Last modified: October 3, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries