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P08818

- CBP2_HORVU

UniProt

P08818 - CBP2_HORVU

Protein

Serine carboxypeptidase 2

Gene

CBP2

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (02 May 2002)
      Previous versions | rss
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    Functioni

    May be involved in the degradation of small peptides (2-5 residues) or in the degradation of storage proteins in the embryo.

    Catalytic activityi

    Preferential release of a C-terminal arginine or lysine residue.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei190 – 1901By similarity
    Active sitei390 – 3901By similarity
    Active sitei443 – 4431By similarity

    GO - Molecular functioni

    1. serine-type carboxypeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Carboxypeptidase, Hydrolase, Protease

    Protein family/group databases

    MEROPSiS10.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine carboxypeptidase 2 (EC:3.4.16.6)
    Alternative name(s):
    CP-MII
    Carboxypeptidase D
    Serine carboxypeptidase II
    Cleaved into the following 2 chains:
    Alternative name(s):
    Serine carboxypeptidase II chain A
    Alternative name(s):
    Serine carboxypeptidase II chain B
    Gene namesi
    Name:CBP2
    Synonyms:CXP;2
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiP08818.

    Subcellular locationi

    Secreted
    Note: Secreted into the endosperm.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 34341 PublicationAdd
    BLAST
    Chaini35 – 294260Serine carboxypeptidase 2 chain APRO_0000004309Add
    BLAST
    Propeptidei295 – 31319Linker peptideBy similarityPRO_0000004310Add
    BLAST
    Chaini314 – 476163Serine carboxypeptidase 2 chain BPRO_0000004311Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi97 ↔ 353Interchain (between A and B chains)By similarity
    Glycosylationi148 – 1481N-linked (GlcNAc...)1 Publication
    Glycosylationi159 – 1591N-linked (GlcNAc...)1 Publication
    Disulfide bondi254 ↔ 266By similarity
    Disulfide bondi290 ↔ 320Interchain (between A and B chains)By similarity
    Glycosylationi291 – 2911N-linked (GlcNAc...)1 Publication
    Modified residuei314 – 3141Blocked amino end (Thr)
    Glycosylationi341 – 3411N-linked (GlcNAc...)1 Publication
    Glycosylationi347 – 3471N-linked (GlcNAc...)1 Publication
    Glycosylationi352 – 3521N-linked (GlcNAc...); partial1 Publication
    Glycosylationi352 – 3521O-linked (GalNAc...); in variant 351-AT-3521 Publication
    Glycosylationi472 – 4721N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Expressioni

    Developmental stagei

    Simultaneously present in aleurone and endosperm between 20 and 30 days postanthesis. Accumulates in the developing grain and is stored in its active form in the mature grain. Also found in the roots and shoots of the growing seedling.

    Gene expression databases

    GenevestigatoriP08818.

    Interactioni

    Subunit structurei

    Carboxypeptidase II is a dimer, where each monomer is composed of two chains linked by a disulfide bond.

    Structurei

    3D structure databases

    ProteinModelPortaliP08818.
    SMRiP08818. Positions 37-292, 316-469.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S10 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view]
    PANTHERiPTHR11802. PTHR11802. 1 hit.
    PfamiPF00450. Peptidase_S10. 1 hit.
    [Graphical view]
    PRINTSiPR00724. CRBOXYPTASEC.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
    PS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08818-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRTTTRRLPP APAAAAVLLA ALTCLLLRPA AVAAAGGHAA DRIVRLPGQP    50
    EVDFDMYSGY ITVDEAAGRS LFYLLQEAPE EAQPAPLVLW LNGGPGCSSV 100
    AYGASEELGA FRVMPRGAGL VLNEYRWNKV ANVLFLDSPA GVGFSYTNTS 150
    SDIYTSGDNR TAHDSYAFLA AWFERFPHYK YREFYVAGES YAGHYVPELS 200
    QLVHRSGNPV INLKGFMVGN GLIDDYHDYV GTFEFWWNHG IVSDDTYRRL 250
    KDACLHDSFI HPSPACDAAT DVATAEQGNI DMYSLYTPVC NISSSSSSSS 300
    LSRRRTRGRY PWLTGSYDPC TERYSTAYYN RRDVQTALHA NVTGAMNYTW 350
    TNCSDTINTH WHDAPRSMLP IYRELIAAGL RIWVFSGDTD AVVPLTATRY 400
    SIGALGLATT TSWYPWYDDL QEVGGWSQVY KGLTLVSVRG AGHEVPLHRP 450
    RQALILFQQF LQGKPMPGRT TNVTVA 476
    Length:476
    Mass (Da):52,625
    Last modified:May 2, 2002 - v2
    Checksum:i18685725B1A6B5E4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811Y → R AA sequence 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti351 – 3522TN → AT.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09602 Genomic DNA. Translation: CAA70815.1.
    PIRiT05701.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09602 Genomic DNA. Translation: CAA70815.1 .
    PIRi T05701.

    3D structure databases

    ProteinModelPortali P08818.
    SMRi P08818. Positions 37-292, 316-469.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S10.005.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P08818.

    Gene expression databases

    Genevestigatori P08818.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view ]
    PANTHERi PTHR11802. PTHR11802. 1 hit.
    Pfami PF00450. Peptidase_S10. 1 hit.
    [Graphical view ]
    PRINTSi PR00724. CRBOXYPTASEC.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00560. CARBOXYPEPT_SER_HIS. 1 hit.
    PS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene family of serine carboxypeptidases in barley."
      Rocher A., Lok F., Cameron-Mills V., von Wettstein D.
      Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Igri.
      Tissue: Etiolated leaf.
    2. "Primary structure of carboxypeptidase II from malted barley."
      Soerensen S.B., Svendsen I., Breddam K.
      Carlsberg Res. Commun. 52:285-295(1987)
      Cited for: PROTEIN SEQUENCE OF 35-294 AND 314-472, GLYCOSYLATION OF VARIANT 351-AT-352.

    Entry informationi

    Entry nameiCBP2_HORVU
    AccessioniPrimary (citable) accession number: P08818
    Secondary accession number(s): P93177
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: May 2, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3