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P08818

- CBP2_HORVU

UniProt

P08818 - CBP2_HORVU

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Protein

Serine carboxypeptidase 2

Gene

CBP2

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in the degradation of small peptides (2-5 residues) or in the degradation of storage proteins in the embryo.

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei190 – 1901By similarity
Active sitei390 – 3901By similarity
Active sitei443 – 4431By similarity

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Protein family/group databases

MEROPSiS10.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase 2 (EC:3.4.16.6)
Alternative name(s):
CP-MII
Carboxypeptidase D
Serine carboxypeptidase II
Cleaved into the following 2 chains:
Alternative name(s):
Serine carboxypeptidase II chain A
Alternative name(s):
Serine carboxypeptidase II chain B
Gene namesi
Name:CBP2
Synonyms:CXP;2
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP08818.

Subcellular locationi

Secreted
Note: Secreted into the endosperm.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 34341 PublicationAdd
BLAST
Chaini35 – 294260Serine carboxypeptidase 2 chain APRO_0000004309Add
BLAST
Propeptidei295 – 31319Linker peptideBy similarityPRO_0000004310Add
BLAST
Chaini314 – 476163Serine carboxypeptidase 2 chain BPRO_0000004311Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi97 ↔ 353Interchain (between A and B chains)By similarity
Glycosylationi148 – 1481N-linked (GlcNAc...)
Glycosylationi159 – 1591N-linked (GlcNAc...)
Disulfide bondi254 ↔ 266By similarity
Disulfide bondi290 ↔ 320Interchain (between A and B chains)By similarity
Glycosylationi291 – 2911N-linked (GlcNAc...)
Modified residuei314 – 3141Blocked amino end (Thr)
Glycosylationi341 – 3411N-linked (GlcNAc...)
Glycosylationi347 – 3471N-linked (GlcNAc...)
Glycosylationi352 – 3521N-linked (GlcNAc...); partial
Glycosylationi352 – 3521O-linked (GalNAc...); in variant 351-AT-352
Glycosylationi472 – 4721N-linked (GlcNAc...)

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Developmental stagei

Simultaneously present in aleurone and endosperm between 20 and 30 days postanthesis. Accumulates in the developing grain and is stored in its active form in the mature grain. Also found in the roots and shoots of the growing seedling.

Gene expression databases

GenevestigatoriP08818.

Interactioni

Subunit structurei

Carboxypeptidase II is a dimer, where each monomer is composed of two chains linked by a disulfide bond.

Structurei

3D structure databases

ProteinModelPortaliP08818.
SMRiP08818. Positions 37-292, 316-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08818-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRTTTRRLPP APAAAAVLLA ALTCLLLRPA AVAAAGGHAA DRIVRLPGQP
60 70 80 90 100
EVDFDMYSGY ITVDEAAGRS LFYLLQEAPE EAQPAPLVLW LNGGPGCSSV
110 120 130 140 150
AYGASEELGA FRVMPRGAGL VLNEYRWNKV ANVLFLDSPA GVGFSYTNTS
160 170 180 190 200
SDIYTSGDNR TAHDSYAFLA AWFERFPHYK YREFYVAGES YAGHYVPELS
210 220 230 240 250
QLVHRSGNPV INLKGFMVGN GLIDDYHDYV GTFEFWWNHG IVSDDTYRRL
260 270 280 290 300
KDACLHDSFI HPSPACDAAT DVATAEQGNI DMYSLYTPVC NISSSSSSSS
310 320 330 340 350
LSRRRTRGRY PWLTGSYDPC TERYSTAYYN RRDVQTALHA NVTGAMNYTW
360 370 380 390 400
TNCSDTINTH WHDAPRSMLP IYRELIAAGL RIWVFSGDTD AVVPLTATRY
410 420 430 440 450
SIGALGLATT TSWYPWYDDL QEVGGWSQVY KGLTLVSVRG AGHEVPLHRP
460 470
RQALILFQQF LQGKPMPGRT TNVTVA
Length:476
Mass (Da):52,625
Last modified:May 2, 2002 - v2
Checksum:i18685725B1A6B5E4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811Y → R AA sequence 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti351 – 3522TN → AT.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09602 Genomic DNA. Translation: CAA70815.1.
PIRiT05701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09602 Genomic DNA. Translation: CAA70815.1 .
PIRi T05701.

3D structure databases

ProteinModelPortali P08818.
SMRi P08818. Positions 37-292, 316-469.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S10.005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P08818.

Gene expression databases

Genevestigatori P08818.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view ]
PANTHERi PTHR11802. PTHR11802. 1 hit.
Pfami PF00450. Peptidase_S10. 1 hit.
[Graphical view ]
PRINTSi PR00724. CRBOXYPTASEC.
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The gene family of serine carboxypeptidases in barley."
    Rocher A., Lok F., Cameron-Mills V., von Wettstein D.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Igri.
    Tissue: Etiolated leaf.
  2. "Primary structure of carboxypeptidase II from malted barley."
    Soerensen S.B., Svendsen I., Breddam K.
    Carlsberg Res. Commun. 52:285-295(1987)
    Cited for: PROTEIN SEQUENCE OF 35-294 AND 314-472, GLYCOSYLATION OF VARIANT 351-AT-352.

Entry informationi

Entry nameiCBP2_HORVU
AccessioniPrimary (citable) accession number: P08818
Secondary accession number(s): P93177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: May 2, 2002
Last modified: October 29, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3