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Protein

Serine carboxypeptidase 2

Gene

CBP2

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the degradation of small peptides (2-5 residues) or in the degradation of storage proteins in the embryo.

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei190By similarity1
Active sitei390By similarity1
Active sitei443By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Protein family/group databases

ESTHERihorvu-cbp2. Carboxypeptidase_S10.
MEROPSiS10.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase 2 (EC:3.4.16.6)
Alternative name(s):
CP-MII
Carboxypeptidase D
Serine carboxypeptidase II
Cleaved into the following 2 chains:
Alternative name(s):
Serine carboxypeptidase II chain A
Alternative name(s):
Serine carboxypeptidase II chain B
Gene namesi
Name:CBP2
Synonyms:CXP;2
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

  • Secreted

  • Note: Secreted into the endosperm.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 341 PublicationAdd BLAST34
ChainiPRO_000000430935 – 294Serine carboxypeptidase 2 chain AAdd BLAST260
PropeptideiPRO_0000004310295 – 313Linker peptideBy similarityAdd BLAST19
ChainiPRO_0000004311314 – 476Serine carboxypeptidase 2 chain BAdd BLAST163

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi97 ↔ 353Interchain (between A and B chains)By similarity
Glycosylationi148N-linked (GlcNAc...)1
Glycosylationi159N-linked (GlcNAc...)1
Disulfide bondi254 ↔ 266By similarity
Disulfide bondi290 ↔ 320Interchain (between A and B chains)By similarity
Glycosylationi291N-linked (GlcNAc...)1
Modified residuei314Blocked amino end (Thr)1
Glycosylationi341N-linked (GlcNAc...)1
Glycosylationi347N-linked (GlcNAc...)1
Glycosylationi352N-linked (GlcNAc...); partial1
Glycosylationi352O-linked (GalNAc...); in variant 351-AT-3521
Glycosylationi472N-linked (GlcNAc...)1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Developmental stagei

Simultaneously present in aleurone and endosperm between 20 and 30 days postanthesis. Accumulates in the developing grain and is stored in its active form in the mature grain. Also found in the roots and shoots of the growing seedling.

Interactioni

Subunit structurei

Carboxypeptidase II is a dimer, where each monomer is composed of two chains linked by a disulfide bond.

Protein-protein interaction databases

STRINGi4513.MLOC_55352.3.

Structurei

3D structure databases

ProteinModelPortaliP08818.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTTTRRLPP APAAAAVLLA ALTCLLLRPA AVAAAGGHAA DRIVRLPGQP
60 70 80 90 100
EVDFDMYSGY ITVDEAAGRS LFYLLQEAPE EAQPAPLVLW LNGGPGCSSV
110 120 130 140 150
AYGASEELGA FRVMPRGAGL VLNEYRWNKV ANVLFLDSPA GVGFSYTNTS
160 170 180 190 200
SDIYTSGDNR TAHDSYAFLA AWFERFPHYK YREFYVAGES YAGHYVPELS
210 220 230 240 250
QLVHRSGNPV INLKGFMVGN GLIDDYHDYV GTFEFWWNHG IVSDDTYRRL
260 270 280 290 300
KDACLHDSFI HPSPACDAAT DVATAEQGNI DMYSLYTPVC NISSSSSSSS
310 320 330 340 350
LSRRRTRGRY PWLTGSYDPC TERYSTAYYN RRDVQTALHA NVTGAMNYTW
360 370 380 390 400
TNCSDTINTH WHDAPRSMLP IYRELIAAGL RIWVFSGDTD AVVPLTATRY
410 420 430 440 450
SIGALGLATT TSWYPWYDDL QEVGGWSQVY KGLTLVSVRG AGHEVPLHRP
460 470
RQALILFQQF LQGKPMPGRT TNVTVA
Length:476
Mass (Da):52,625
Last modified:May 2, 2002 - v2
Checksum:i18685725B1A6B5E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181Y → R AA sequence (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti351 – 352TN → AT.2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09602 Genomic DNA. Translation: CAA70815.1.
PIRiT05701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09602 Genomic DNA. Translation: CAA70815.1.
PIRiT05701.

3D structure databases

ProteinModelPortaliP08818.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4513.MLOC_55352.3.

Protein family/group databases

ESTHERihorvu-cbp2. Carboxypeptidase_S10.
MEROPSiS10.005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBP2_HORVU
AccessioniPrimary (citable) accession number: P08818
Secondary accession number(s): P93177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: May 2, 2002
Last modified: October 5, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.