P08800 (UGPA1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UTP--glucose-1-phosphate uridylyltransferase 1
EC=2.7.7.9

Alternative name(s):
UDP-glucose pyrophosphorylase 1
Short name=UDPGP 1
Short name=UGPase 1

Gene names

Name:	uppA
ORF Names:	DDB_G0289875

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a central role as a glucosyl donor in cellular metabolic pathways. During development the lack of the enzyme activity leads to cell death and lysis. Strains which lack UDPG pyrophosphorylase accomplish early developmental events but are unable to culminate. The enzyme affects the growth rate of the cells but is not essential for growth. Ref.1
Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Pathway	Carbohydrate metabolism; galactose metabolism.
Subunit structure	Homooctamer By similarity.
Developmental stage	Expressed in both prestalk and prespore cells. Expression becomes strong after 12 hours of development and peaks at 16 hours. Ref.5
Induction	By cAMP through transmembrane signal transduction. Ref.4
Sequence similarities	Belongs to the UDPGP type 1 family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	galactose metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	UTP--glucose-1-phosphate uridylyltransferase 1		PRO_0000185755
Regions
	Region	460 – 511	52	Oligomerization By similarity
Sites
	Active site	399	1	By similarity
	Metal binding	140	1	Magnesium By similarity
	Metal binding	264	1	Magnesium By similarity
Experimental info
	Sequence conflict	28	1	N → D in CAA68340. Ref.1
	Sequence conflict	28	1	N → D in AAA84450. Ref.2
	Sequence conflict	28	1	N → D in AAA33271. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P08800 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: B6732C5C38218733	FASTA	511	57,885
	10 20 30 40 50 60 MTDTATSKAT VERPKLQSTG SLHSLFKNVD LFSENDEELY PPLQHGARFA APIEDSTLLA 70 80 90 100 110 120 LGMKPDELKA FQKQRHAYIN KDQIYTDEIK IPNKTEMVDY HQLHLVSPID QSNASRLLNK 130 140 150 160 170 180 LVVIKLNGGL GNSMGCKTAK STMEIAPGVT FLDMAVAHIE QINQDYNVDV PLVIMNSYKT 190 200 210 220 230 240 HNETNKVIEK YKTHKVSIKT FQQSMFPKMY KDTLNLVPKP NTPMNPKEWY PPGSGDIFRS 250 260 270 280 290 300 LQRSGLIDEF LAAGKEYIFI SNVENLGSII DLQVLNHIHL QKIEFGLEVT NRINTDSTGG 310 320 330 340 350 360 ILMSYKDKLH LLELSQVKPE KLKIFKDFKL WNTNNIWVNL KSVSNLIKED KLDLDWIVNY 370 380 390 400 410 420 PLENHKAMVQ LETPAGMGIQ NFKNSVAIFV PRDRYRPIKS TSQLLVAQSN IFQFDHGQVK 430 440 450 460 470 480 LNSKREGQDV PLVKLGEEFS TVSDYEKRFK SIPDLLELDH LTVSGDVYFG SRITLKGTVI 490 500 510 IVANHGERVD IPDGVVLENK VLSGTLRILD H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and sequence of a UDP glucose pyrophosphorylase gene of Dictyostelium discoideum." Ragheb J.A., Dottin R.P. Nucleic Acids Res. 15:3891-3906(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Transmembrane signal transduction regulates gene expression in Dictyostelium discoideum." Pavlovic J., Haribabu B., Dottin R.P. Dev. Genet. 9:371-382(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
[4]	"Identification of a signal transduction response sequence element necessary for induction of a Dictyostelium discoideum gene by extracellular cyclic AMP." Pavlovic J., Haribabu B., Dottin R.P. Mol. Cell. Biol. 9:4660-4669(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30, INDUCTION.
[5]	"A second UDP-glucose pyrophosphorylase is required for differentiation and development in Dictyostelium discoideum." Bishop J.D., Moon B.C., Harrow F., Ratner D., Gomer R.H., Dottin R.P., Brazill D.T. J. Biol. Chem. 277:32430-32437(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00145 Genomic DNA. Translation: CAA68340.1. AAFI02000149 Genomic DNA. Translation: EAL62450.1. M27639 Genomic DNA. Translation: AAA84450.1. M30467 Genomic DNA. Translation: AAA33271.1.
PIR	XNDOU. S07383.
RefSeq	XP_635982.1. XM_630890.1.
3D structure databases
ProteinModelPortal	P08800.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191527.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191527; DDB0191527; DDB_G0289875.
GeneID	8627397.
KEGG	ddi:DDB_G0289875.
Organism-specific databases
dictyBase	DDB_G0289875. uppA.
Phylogenomic databases
eggNOG	COG4284.
OMA	NDEELYP.
ProtClustDB	CLSZ2429721.
Enzyme and pathway databases
UniPathway	UPA00214.
Family and domain databases
InterPro	IPR002618. UDPGP_trans. IPR016267. UDPGP_trans_subgr. [Graphical view]
PANTHER	PTHR11952. PTHR11952. 1 hit. PTHR11952:SF1. PTHR11952:SF1. 1 hit.
Pfam	PF01704. UDPGP. 1 hit. [Graphical view]
PIRSF	PIRSF000806. UDPGP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UGPA1_DICDI

Accession

Primary (citable) accession number: P08800
Secondary accession number(s): Q54GT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)