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P08800

- UGPA1_DICDI

UniProt

P08800 - UGPA1_DICDI

Protein

UTP--glucose-1-phosphate uridylyltransferase 1

Gene

uppA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Plays a central role as a glucosyl donor in cellular metabolic pathways. During development the lack of the enzyme activity leads to cell death and lysis. Strains which lack UDPG pyrophosphorylase accomplish early developmental events but are unable to culminate. The enzyme affects the growth rate of the cells but is not essential for growth.1 Publication

    Catalytic activityi

    UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi140 – 1401MagnesiumBy similarity
    Metal bindingi264 – 2641MagnesiumBy similarity
    Active sitei399 – 3991By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. UTP:glucose-1-phosphate uridylyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. galactose metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_176277. Glycogen synthesis.
    UniPathwayiUPA00214.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase 1 (EC:2.7.7.9)
    Alternative name(s):
    UDP-glucose pyrophosphorylase 1
    Short name:
    UDPGP 1
    Short name:
    UGPase 1
    Gene namesi
    Name:uppA
    ORF Names:DDB_G0289875
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0289875. uppA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 511511UTP--glucose-1-phosphate uridylyltransferase 1PRO_0000185755Add
    BLAST

    Proteomic databases

    PRIDEiP08800.

    Expressioni

    Developmental stagei

    Expressed in both prestalk and prespore cells. Expression becomes strong after 12 hours of development and peaks at 16 hours.1 Publication

    Inductioni

    By cAMP through transmembrane signal transduction.1 Publication

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0191527.

    Structurei

    3D structure databases

    ProteinModelPortaliP08800.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni460 – 51152OligomerizationBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the UDPGP type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG4284.
    OMAiNDEELYP.
    PhylomeDBiP08800.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR029044. Nucleotide-diphossugar_trans.
    IPR016267. UDPGP_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view]
    PANTHERiPTHR11952. PTHR11952. 1 hit.
    PfamiPF01704. UDPGP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000806. UDPGP. 1 hit.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P08800-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDTATSKAT VERPKLQSTG SLHSLFKNVD LFSENDEELY PPLQHGARFA    50
    APIEDSTLLA LGMKPDELKA FQKQRHAYIN KDQIYTDEIK IPNKTEMVDY 100
    HQLHLVSPID QSNASRLLNK LVVIKLNGGL GNSMGCKTAK STMEIAPGVT 150
    FLDMAVAHIE QINQDYNVDV PLVIMNSYKT HNETNKVIEK YKTHKVSIKT 200
    FQQSMFPKMY KDTLNLVPKP NTPMNPKEWY PPGSGDIFRS LQRSGLIDEF 250
    LAAGKEYIFI SNVENLGSII DLQVLNHIHL QKIEFGLEVT NRINTDSTGG 300
    ILMSYKDKLH LLELSQVKPE KLKIFKDFKL WNTNNIWVNL KSVSNLIKED 350
    KLDLDWIVNY PLENHKAMVQ LETPAGMGIQ NFKNSVAIFV PRDRYRPIKS 400
    TSQLLVAQSN IFQFDHGQVK LNSKREGQDV PLVKLGEEFS TVSDYEKRFK 450
    SIPDLLELDH LTVSGDVYFG SRITLKGTVI IVANHGERVD IPDGVVLENK 500
    VLSGTLRILD H 511
    Length:511
    Mass (Da):57,885
    Last modified:December 4, 2007 - v2
    Checksum:iB6732C5C38218733
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281N → D in CAA68340. (PubMed:3035502)Curated
    Sequence conflicti28 – 281N → D in AAA84450. (PubMed:15875012)Curated
    Sequence conflicti28 – 281N → D in AAA33271. (PubMed:2854024)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00145 Genomic DNA. Translation: CAA68340.1.
    AAFI02000149 Genomic DNA. Translation: EAL62450.1.
    M27639 Genomic DNA. Translation: AAA84450.1.
    M30467 Genomic DNA. Translation: AAA33271.1.
    PIRiS07383. XNDOU.
    RefSeqiXP_635982.1. XM_630890.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191527; DDB0191527; DDB_G0289875.
    GeneIDi8627397.
    KEGGiddi:DDB_G0289875.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00145 Genomic DNA. Translation: CAA68340.1 .
    AAFI02000149 Genomic DNA. Translation: EAL62450.1 .
    M27639 Genomic DNA. Translation: AAA84450.1 .
    M30467 Genomic DNA. Translation: AAA33271.1 .
    PIRi S07383. XNDOU.
    RefSeqi XP_635982.1. XM_630890.1.

    3D structure databases

    ProteinModelPortali P08800.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191527.

    Proteomic databases

    PRIDEi P08800.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191527 ; DDB0191527 ; DDB_G0289875 .
    GeneIDi 8627397.
    KEGGi ddi:DDB_G0289875.

    Organism-specific databases

    dictyBasei DDB_G0289875. uppA.

    Phylogenomic databases

    eggNOGi COG4284.
    OMAi NDEELYP.
    PhylomeDBi P08800.

    Enzyme and pathway databases

    UniPathwayi UPA00214 .
    Reactomei REACT_176277. Glycogen synthesis.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR029044. Nucleotide-diphossugar_trans.
    IPR016267. UDPGP_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view ]
    PANTHERi PTHR11952. PTHR11952. 1 hit.
    Pfami PF01704. UDPGP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000806. UDPGP. 1 hit.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and sequence of a UDP glucose pyrophosphorylase gene of Dictyostelium discoideum."
      Ragheb J.A., Dottin R.P.
      Nucleic Acids Res. 15:3891-3906(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: AX3.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Transmembrane signal transduction regulates gene expression in Dictyostelium discoideum."
      Pavlovic J., Haribabu B., Dottin R.P.
      Dev. Genet. 9:371-382(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
    4. "Identification of a signal transduction response sequence element necessary for induction of a Dictyostelium discoideum gene by extracellular cyclic AMP."
      Pavlovic J., Haribabu B., Dottin R.P.
      Mol. Cell. Biol. 9:4660-4669(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30, INDUCTION.
    5. "A second UDP-glucose pyrophosphorylase is required for differentiation and development in Dictyostelium discoideum."
      Bishop J.D., Moon B.C., Harrow F., Ratner D., Gomer R.H., Dottin R.P., Brazill D.T.
      J. Biol. Chem. 277:32430-32437(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
      Strain: AX4.

    Entry informationi

    Entry nameiUGPA1_DICDI
    AccessioniPrimary (citable) accession number: P08800
    Secondary accession number(s): Q54GT7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3