Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P08800 (UGPA1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UTP--glucose-1-phosphate uridylyltransferase 1

EC=2.7.7.9
Alternative name(s):
UDP-glucose pyrophosphorylase 1
Short name=UDPGP 1
Short name=UGPase 1
Gene names
Name:uppA
ORF Names:DDB_G0289875
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a central role as a glucosyl donor in cellular metabolic pathways. During development the lack of the enzyme activity leads to cell death and lysis. Strains which lack UDPG pyrophosphorylase accomplish early developmental events but are unable to culminate. The enzyme affects the growth rate of the cells but is not essential for growth. Ref.1

Catalytic activity

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Pathway

Carbohydrate metabolism; galactose metabolism.

Subunit structure

Homooctamer By similarity.

Developmental stage

Expressed in both prestalk and prespore cells. Expression becomes strong after 12 hours of development and peaks at 16 hours. Ref.5

Induction

By cAMP through transmembrane signal transduction. Ref.4

Sequence similarities

Belongs to the UDPGP type 1 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 511511UTP--glucose-1-phosphate uridylyltransferase 1
PRO_0000185755

Regions

Region460 – 51152Oligomerization By similarity

Sites

Active site3991 By similarity
Metal binding1401Magnesium By similarity
Metal binding2641Magnesium By similarity

Experimental info

Sequence conflict281N → D in CAA68340. Ref.1
Sequence conflict281N → D in AAA84450. Ref.2
Sequence conflict281N → D in AAA33271. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P08800 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: B6732C5C38218733

FASTA51157,885
        10         20         30         40         50         60 
MTDTATSKAT VERPKLQSTG SLHSLFKNVD LFSENDEELY PPLQHGARFA APIEDSTLLA 

        70         80         90        100        110        120 
LGMKPDELKA FQKQRHAYIN KDQIYTDEIK IPNKTEMVDY HQLHLVSPID QSNASRLLNK 

       130        140        150        160        170        180 
LVVIKLNGGL GNSMGCKTAK STMEIAPGVT FLDMAVAHIE QINQDYNVDV PLVIMNSYKT 

       190        200        210        220        230        240 
HNETNKVIEK YKTHKVSIKT FQQSMFPKMY KDTLNLVPKP NTPMNPKEWY PPGSGDIFRS 

       250        260        270        280        290        300 
LQRSGLIDEF LAAGKEYIFI SNVENLGSII DLQVLNHIHL QKIEFGLEVT NRINTDSTGG 

       310        320        330        340        350        360 
ILMSYKDKLH LLELSQVKPE KLKIFKDFKL WNTNNIWVNL KSVSNLIKED KLDLDWIVNY 

       370        380        390        400        410        420 
PLENHKAMVQ LETPAGMGIQ NFKNSVAIFV PRDRYRPIKS TSQLLVAQSN IFQFDHGQVK 

       430        440        450        460        470        480 
LNSKREGQDV PLVKLGEEFS TVSDYEKRFK SIPDLLELDH LTVSGDVYFG SRITLKGTVI 

       490        500        510 
IVANHGERVD IPDGVVLENK VLSGTLRILD H 

« Hide

References

« Hide 'large scale' references
[1]"Structure and sequence of a UDP glucose pyrophosphorylase gene of Dictyostelium discoideum."
Ragheb J.A., Dottin R.P.
Nucleic Acids Res. 15:3891-3906(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: AX3.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Transmembrane signal transduction regulates gene expression in Dictyostelium discoideum."
Pavlovic J., Haribabu B., Dottin R.P.
Dev. Genet. 9:371-382(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
[4]"Identification of a signal transduction response sequence element necessary for induction of a Dictyostelium discoideum gene by extracellular cyclic AMP."
Pavlovic J., Haribabu B., Dottin R.P.
Mol. Cell. Biol. 9:4660-4669(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30, INDUCTION.
[5]"A second UDP-glucose pyrophosphorylase is required for differentiation and development in Dictyostelium discoideum."
Bishop J.D., Moon B.C., Harrow F., Ratner D., Gomer R.H., Dottin R.P., Brazill D.T.
J. Biol. Chem. 277:32430-32437(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00145 Genomic DNA. Translation: CAA68340.1.
AAFI02000149 Genomic DNA. Translation: EAL62450.1.
M27639 Genomic DNA. Translation: AAA84450.1.
M30467 Genomic DNA. Translation: AAA33271.1.
PIRXNDOU. S07383.
RefSeqXP_635982.1. XM_630890.1.

3D structure databases

ProteinModelPortalP08800.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191527.

Proteomic databases

PRIDEP08800.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191527; DDB0191527; DDB_G0289875.
GeneID8627397.
KEGGddi:DDB_G0289875.

Organism-specific databases

dictyBaseDDB_G0289875. uppA.

Phylogenomic databases

eggNOGCOG4284.
OMANDEELYP.
PhylomeDBP08800.

Enzyme and pathway databases

UniPathwayUPA00214.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans.
IPR016267. UDPGP_trans_subgr.
[Graphical view]
PANTHERPTHR11952. PTHR11952. 1 hit.
PfamPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFPIRSF000806. UDPGP. 1 hit.
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Entry information

Entry nameUGPA1_DICDI
AccessionPrimary (citable) accession number: P08800
Secondary accession number(s): Q54GT7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: December 4, 2007
Last modified: June 11, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase