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Protein

UTP--glucose-1-phosphate uridylyltransferase 1

Gene

uppA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a central role as a glucosyl donor in cellular metabolic pathways. During development the lack of the enzyme activity leads to cell death and lysis. Strains which lack UDPG pyrophosphorylase accomplish early developmental events but are unable to culminate. The enzyme affects the growth rate of the cells but is not essential for growth.1 Publication

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi140MagnesiumBy similarity1
Binding sitei140UTPBy similarity1
Binding sitei203UTPBy similarity1
Binding sitei233UTP; via amide nitrogenBy similarity1
Metal bindingi264MagnesiumBy similarity1
Binding sitei264UTPBy similarity1
Active sitei399By similarity1
Binding sitei399UTPBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • UTP:glucose-1-phosphate uridylyltransferase activity Source: dictyBase

GO - Biological processi

  • culmination involved in sorocarp development Source: dictyBase
  • galactose metabolic process Source: UniProtKB-UniPathway
  • glycogen metabolic process Source: GO_Central
  • UDP-glucose metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase 1 (EC:2.7.7.9)
Alternative name(s):
UDP-glucose pyrophosphorylase 1
Short name:
UDPGP 1
Short name:
UGPase 1
Gene namesi
Name:uppA
ORF Names:DDB_G0289875
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0289875. uppA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857551 – 511UTP--glucose-1-phosphate uridylyltransferase 1Add BLAST511

Proteomic databases

PaxDbiP08800.

Expressioni

Developmental stagei

Expressed in both prestalk and prespore cells. Expression becomes strong after 12 hours of development and peaks at 16 hours.1 Publication

Inductioni

By cAMP through transmembrane signal transduction.1 Publication

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191527.

Structurei

3D structure databases

ProteinModelPortaliP08800.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 129UTP bindingBy similarity4
Regioni128 – 129Substrate bindingBy similarity2
Regioni262 – 264Substrate bindingBy similarity3
Regioni460 – 511OligomerizationBy similarityAdd BLAST52

Sequence similaritiesi

Belongs to the UDPGP type 1 family.Curated

Phylogenomic databases

eggNOGiKOG2638. Eukaryota.
COG4284. LUCA.
InParanoidiP08800.
KOiK00963.
OMAiTNRINTD.
PhylomeDBiP08800.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
IPR016267. UDPGP_trans.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P08800-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTATSKAT VERPKLQSTG SLHSLFKNVD LFSENDEELY PPLQHGARFA
60 70 80 90 100
APIEDSTLLA LGMKPDELKA FQKQRHAYIN KDQIYTDEIK IPNKTEMVDY
110 120 130 140 150
HQLHLVSPID QSNASRLLNK LVVIKLNGGL GNSMGCKTAK STMEIAPGVT
160 170 180 190 200
FLDMAVAHIE QINQDYNVDV PLVIMNSYKT HNETNKVIEK YKTHKVSIKT
210 220 230 240 250
FQQSMFPKMY KDTLNLVPKP NTPMNPKEWY PPGSGDIFRS LQRSGLIDEF
260 270 280 290 300
LAAGKEYIFI SNVENLGSII DLQVLNHIHL QKIEFGLEVT NRINTDSTGG
310 320 330 340 350
ILMSYKDKLH LLELSQVKPE KLKIFKDFKL WNTNNIWVNL KSVSNLIKED
360 370 380 390 400
KLDLDWIVNY PLENHKAMVQ LETPAGMGIQ NFKNSVAIFV PRDRYRPIKS
410 420 430 440 450
TSQLLVAQSN IFQFDHGQVK LNSKREGQDV PLVKLGEEFS TVSDYEKRFK
460 470 480 490 500
SIPDLLELDH LTVSGDVYFG SRITLKGTVI IVANHGERVD IPDGVVLENK
510
VLSGTLRILD H
Length:511
Mass (Da):57,885
Last modified:December 4, 2007 - v2
Checksum:iB6732C5C38218733
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28N → D in CAA68340 (PubMed:3035502).Curated1
Sequence conflicti28N → D in AAA84450 (PubMed:15875012).Curated1
Sequence conflicti28N → D in AAA33271 (PubMed:2854024).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00145 Genomic DNA. Translation: CAA68340.1.
AAFI02000149 Genomic DNA. Translation: EAL62450.1.
M27639 Genomic DNA. Translation: AAA84450.1.
M30467 Genomic DNA. Translation: AAA33271.1.
PIRiS07383. XNDOU.
RefSeqiXP_635982.1. XM_630890.1.

Genome annotation databases

EnsemblProtistsiEAL62450; EAL62450; DDB_G0289875.
GeneIDi8627397.
KEGGiddi:DDB_G0289875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00145 Genomic DNA. Translation: CAA68340.1.
AAFI02000149 Genomic DNA. Translation: EAL62450.1.
M27639 Genomic DNA. Translation: AAA84450.1.
M30467 Genomic DNA. Translation: AAA33271.1.
PIRiS07383. XNDOU.
RefSeqiXP_635982.1. XM_630890.1.

3D structure databases

ProteinModelPortaliP08800.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191527.

Proteomic databases

PaxDbiP08800.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL62450; EAL62450; DDB_G0289875.
GeneIDi8627397.
KEGGiddi:DDB_G0289875.

Organism-specific databases

dictyBaseiDDB_G0289875. uppA.

Phylogenomic databases

eggNOGiKOG2638. Eukaryota.
COG4284. LUCA.
InParanoidiP08800.
KOiK00963.
OMAiTNRINTD.
PhylomeDBiP08800.

Enzyme and pathway databases

UniPathwayiUPA00214.

Miscellaneous databases

PROiP08800.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
IPR016267. UDPGP_trans.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUGPA1_DICDI
AccessioniPrimary (citable) accession number: P08800
Secondary accession number(s): Q54GT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: December 4, 2007
Last modified: November 30, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.