P08796 (CSA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Contact site A protein
Short name=CSA

Alternative name(s):
Cell adhesion molecule gp80
Membrane-associated glycoprotein gp80

Gene names

Name:	csaA
ORF Names:	DDB_G0289073

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This cell-surface glycoprotein mediates cell-cell binding via homophilic interaction.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor. Note: Attached to the membrane by a GPI-anchor that contains a phosphoceramide moiety. Such anchor mediates a fast and long persistence cell adhesion of the protein. Ref.9
Developmental stage	Restricted to the aggregation stage of development of D.discoideum.
Domain	The C-terminal region contains clusters of proline regularly alternating with a hydroxyamino acid. This domain might act as a spacer to elevate sites active in cell contact into the extracellular space. Ref.8
Post-translational modification	Phosphorylated on serine and N-glycosylated with two types of oligosaccharide chains. The GPI-like-anchor contains a phosphoceramide group, rather than a phosphatidyl group.
Miscellaneous	The expression of this stringently regulated protein during cell development is mediated through cell-surface cAMP receptors.
Sequence similarities	Contains 2 IPT/TIG domains.
Caution	The Dictyosteliida are known to produce a glycosylsphingolipidinositol anchor (GPI-like-anchor). It has not been established whether Dictyosteliida make a glycosylphosphatidylinositol anchor (GPI-anchor) also, and whether their GPI-like-anchor modifications can be interconverted with GPI-anchor modifications in a resculpting process. It has not been established that the GPI-like-anchor modification in Dictyosteliida utilizes the same sequence motif.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Repeat Signal
PTM	GPI-anchor Glycoprotein Lipoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	aggregation involved in sorocarp development Traceable author statement PubMed 15366765. Source: dictyBase cell-cell adhesion Inferred from mutant phenotype PubMed 6191197. Source: dictyBase cortical actin cytoskeleton organization Traceable author statement PubMed 15366765. Source: dictyBase protein homooligomerization Traceable author statement PubMed 15366765. Source: dictyBase
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW external side of plasma membrane Inferred from direct assay PubMed 6191197. Source: dictyBase extracellular space Inferred from direct assay PubMed 20422638. Source: dictyBase
Molecular_function	protein homodimerization activity Traceable author statement PubMed 15366765. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.5 Ref.6
	Chain	20 – 492	473	Contact site A protein		PRO_0000021010
	Propeptide	493 – 514	22	Removed in mature form Potential		PRO_0000021011
Regions
	Domain	21 – 104	84	IPT/TIG 1
	Domain	191 – 283	93	IPT/TIG 2
	Repeat	462 – 469	8	1
	Repeat	472 – 479	8	2
	Region	20 – 453	434	Globular Potential
	Region	462 – 479	18	2 X 8 AA repeats, Pro-rich
	Compositional bias	454 – 485	32	Pro-rich (hinge)
Amino acid modifications
	Lipidation	492	1	GPI-like-anchor amidated serine Potential
	Glycosylation	128	1	N-linked (GlcNAc...) Potential
	Glycosylation	137	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Glycosylation	294	1	N-linked (GlcNAc...) Potential
	Glycosylation	399	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	216	1	G → V in AAA33212. Ref.2
	Sequence conflict	216	1	G → V in CAA47110. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P08796 [UniParc]. Last modified December 4, 2007. Version 3. Checksum: DB1BA39B56AAD878	FASTA	514	53,655
	10 20 30 40 50 60 MKFLLVLIIL YNILNSAHSA PTITAVSNGK FGVPTYITIT GTGFTGTPVV TIGGQTCDPV 70 80 90 100 110 120 IVANTASLQC QFSAQLAPGN SNFDVIVKVG GVPSTGGNGL FKYTPPTLST IFPNNGRIGM 130 140 150 160 170 180 ILVDGPSNIS GYKLNVNDSI NSAMLSVTAD SVSPTIYFLV PNTIAGGLLN LELIQPFGFS 190 200 210 220 230 240 TIVTSKSVFS PTITSITPLA FDLTPTNVTV TGKYFGTTAS VTMGSHIYTG LTVQDDGTNC 250 260 270 280 290 300 HVIFTTRSVY ESSNTITAKA STGVDMIYLD NQGNQQPITF TYNPPTITST KQVNDSVEIS 310 320 330 340 350 360 TTNTGTDFTQ ISLTMGTSSP TNLVITGTNE KIVITLPHAL PEGEIQFNLK AGISNVVTST 370 380 390 400 410 420 LLVTPVINSV TQAPHNGGSI TISGIFLNNA HVSIVVDQNT TDIVCAPDSN GESIICPVEA 430 440 450 460 470 480 GSGTINLVVT NYKNFASDPT IKTEATTSTT YTIPDTPTPT DTATPSPTPT ETATPSPTPK 490 500 510 PTSTPEETEA PSSATTLISP LSLIVIFISF VLLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence and transcript regulation of a cell adhesion protein from aggregating Dictyostelium cells." Noegel A., Gerisch G., Stadler J., Westphal M. EMBO J. 5:1473-1476(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular mechanisms of cell-cell interaction in Dictyostelium discoideum." Siu C.-H., Wong L.M., Lam T.Y., Kamboj R.K., Choi A., Cho A. Biochem. Cell Biol. 66:1089-1099(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Identification of a unique cAMP-response element in the gene encoding the cell adhesion molecule gp80 in Dictyostelium discoideum." Desbarats L., Lam T.Y., Wong L.M., Siu C.-H. J. Biol. Chem. 267:19655-19664(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX2.
[4]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[5]	"Improved purification and N-terminal amino acid sequence determination of the contact site A glycoprotein of Dictyostelium discoideum." Stadler J., Bordier C., Lottspeich F., Henschen A., Gerisch G. Hoppe-Seyler's Z. Physiol. Chem. 363:771-776(1982) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-46.
[6]	"Cloning of cDNA for the contact site A glycoprotein of Dictyostelium discoideum." Wong L.M., Siu C.-H. Proc. Natl. Acad. Sci. U.S.A. 83:4248-4252(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-49.
[7]	"Identification of an octapeptide involved in homophilic interaction of the cell adhesion molecule gp80 of Dictyostelium discoideum." Kamboj R.K., Gariepy J., Siu C.-H. Cell 59:615-625(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 132-139.
[8]	"Mapping of a cell-binding domain in the cell adhesion molecule gp80 of Dictyostelium discoideum." Kamboj R.K., Wong L.M., Lam T.Y., Siu C.-H. J. Cell Biol. 107:1835-1843(1988) [PubMed] [Europe PMC] [Abstract] Cited for: CELL-BINDING DOMAIN.
[9]	"The contact site A glycoprotein of Dictyostelium discoideum carries a phospholipid anchor of a novel type." Stadler J., Keenan T.W., Bauer G., Gerisch G. EMBO J. 8:371-377(1989) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04004 mRNA. Translation: CAA27634.1. M36545 mRNA. Translation: AAA33212.1. X66483 Genomic DNA. Translation: CAA47110.1. AAFI02000130 Genomic DNA. Translation: EAL62886.1.
PIR	A44100. A31643. S22066.
RefSeq	XP_636399.1. XM_631307.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191156.
Proteomic databases
PRIDE	P08796.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191156; DDB0191156; DDB_G0289073.
GeneID	8626958.
KEGG	ddi:DDB_G0289073.
Organism-specific databases
dictyBase	DDB_G0289073. csaA.
Phylogenomic databases
eggNOG	NOG12793.
OMA	FTYNPPT.
ProtClustDB	CLSZ2429816.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
SUPFAM	SSF81296. Ig_E-set. 2 hits.
ProtoNet	Search...

Entry information

Entry name

CSA_DICDI

Accession

Primary (citable) accession number: P08796
Secondary accession number(s): P19408, Q54I06

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)