Reviewed, UniProtKB/Swiss-Prot P08793 (XDH_CALVI)

Names and origin

Protein names

Recommended name:
    Xanthine dehydrogenase
      Short name=XD
    EC=1.17.1.4

Gene names

Name:

XDH

Organism

Calliphora vicina (Blue blowfly) (Calliphora erythrocephala)

Taxonomic identifier

7373 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Oestroidea › Calliphoridae › Calliphorinae › Calliphora

Protein attributes

Sequence length	1353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH.
Cofactor	Binds 2 2Fe-2S clusters. FAD. Molybdopterin.
Subunit structure	Homodimer.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the xanthine dehydrogenase family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	2Fe-2S FAD Flavoprotein Iron Iron-sulfur Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW xanthine dehydrogenase activity Inferred from electronic annotation. Source: EC xanthine oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1353	1353	Xanthine dehydrogenase		PRO_0000166078
Regions
	Domain	17 – 104	88	2Fe-2S ferredoxin-type
	Domain	245 – 434	190	FAD-binding PCMH-type
Sites
	Metal binding	50	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	56	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	61	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	64	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08793-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 7120361C57C3E297	FASTA	1,353	150,209
	10 20 30 40 50 60 MTQEHNAAVL DLNPTFSTLI FFVNGKKVID TNPDPECTLL TYLREKLRLC GTKLGCGEGG 70 80 90 100 110 120 CGACTVMISR IDTLTNRIKH IAVNACLTPV CAMHGSAVTT VEGIGSTRTR LHPVQERLAK 130 140 150 160 170 180 AHGSQCGFCT PGIVMSMYAL LRNLSQPSMK DLEIAFQGNL CRCTGYRPIL EGYKTFTKEF 190 200 210 220 230 240 GCAMGDKCCK VNGNKCGEGM ENGGDMVDDK LFEKSEFVPF DPSQEPIFPP ELQLNKDWDS 250 260 270 280 290 300 QTLVYKGERA TWYRPGNLED LLKIKAQFPE AKLVVGNTEI GVEVKFKHFL YPVLVNPTKV 310 320 330 340 350 360 KEMIDVQELE DSIYFGASVS LMDIDRILRS SIEKLPEHQT RFFQCAVNML HYFAGKQIRN 370 380 390 400 410 420 VASLGGNIMT GSPISDMNPV LMAGAVKFKV AKYVEGQIKY REVCMASGFF TGYRKNVIEP 430 440 450 460 470 480 TEILVGLYFP KTLEHQYVVA FKQAKRRDDD IAIVNAAINV FIDPRSITVD KVYMAFGGMA 490 500 510 520 530 540 PTTVLATRTA DIMVKQQWNK VLMERVVENL CAELPLAPSA PGGMIAYRRS LVVSLFFKAY 550 560 570 580 590 600 LTITQQLIKS GILPQDSLPQ EELSGSDVFH TPALKSAQLF EKVSNKQSEC DPIGRPKIHA 610 620 630 640 650 660 SALKQATGEA IYCDDMPRME NELYLALVLS TKAHAKILSI DASEALAMPG VHAFFSSKDI 670 680 690 700 710 720 TQHENEVGPV FHDEEVFASD MVYCQGQVIG AIAADNPNFS SKTARKVTIE YEDIKPVIIT 730 740 750 760 770 780 IEQAIEHKSY FPDYPRFTEI GDVEKAFSEA DHVYEGSCRM GGQEHFYLET HASLAVPRDS 790 800 810 820 830 840 DEIEIFCSTQ HPSEVQKLVA HVLSTSAHRV VCRAKRLGGG FGGKESRAIA VALPVALACH 850 860 870 880 890 900 RLRRPIRCML DRDEDMMITG TRHPFLFKYK IAFTSEGRLT GCYIECYNNA GWSMDLSFSV 910 920 930 940 950 960 LERAMFHFEN CYKIPNIKVG GWVCKTNLPS NTAFRGFGGP QGMFAGEHII RDVARILGKD 970 980 990 1000 1010 1020 YLEIMKQNFY KEGDITHYQQ KLDNFPIEKC FYDCLQQSNY YQKRKEIEEF NRNHRWRKRG 1030 1040 1050 1060 1070 1080 ISLVPTKYGI AFGVSHLNQA GALINIYADG SVLLSHGGVE IGQGLHTKMI QCCARALQIP 1090 1100 1110 1120 1130 1140 IEFIHISETA TDKVPNTSPT AASSGSDLNG MAVLDACEKL NKRLAPIKEA NPNGSWTEWI 1150 1160 1170 1180 1190 1200 NKAYFERVSL SATGFYRMPD IGYDPVQNPN ALMYNYFTNG VGSSIVEIDC LTGDHQVLST 1210 1220 1230 1240 1250 1260 DIVMDIGSSL NPAIDIGQIE GAFMQGYGLF TLEEMIYSPQ GVLYSRGPGM YKLPGFADIP 1270 1280 1290 1300 1310 1320 GEFNVTILTG AANPRAVYSS KAVGEPPLFI GCSVFFAIKE AITSARLMNG LSEDFKLESP 1330 1340 1350 ATSARIRMAC QDEFTNLIEQ PPAGSYVPWN IVP

References

[1]	"Divergence of the nucleotide sequences encoding xanthine dehydrogenase in Calliphora vicina and Drosophila melanogaster." Houde M., Tiveron M.C., Bregegere F. Gene 85:391-402(1989) [PubMed: 2516831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Cloning and partial characterization of the xanthine dehydrogenase gene of Calliphora vicina, a distant relative of Drosophila melanogaster." Rocher-Chambonnet C., Berreur P., Houde M., Tiveron M.C., Lepesant J.-A., Bregegere F. Gene 59:201-212(1987) [PubMed: 2830167] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 208-367.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07229 mRNA. Translation: CAA30189.1. X07323, X07324, X07325 Genomic DNA. Translation: CAA30281.1. M18423 Genomic DNA. Translation: AAA27879.1.
PIR	JQ0407.
3D structure databases
HSSP	HSSP built from PDB template 1FO4 based on UniProtKB P80457.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.17.1.4. 2654.
Family and domain databases
InterPro	IPR002888. 2Fe-2S_bd. IPR006058. 2Fe2S_fd_BS. IPR000674. Ald_Oxase/Xan_DH_a/b. IPR016208. Ald_Oxase/xanthine_DH. IPR008274. AldOxase/xan_DH_Mopterin-bd. IPR012675. b-grasp_ferredoxin-like. IPR005107. CO_DH_flav_C. IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR001041. Ferredoxin. IPR002346. Mopterin_DH_FAD-bd. IPR000572. OxRdtase_Mopterin-bd. IPR014307. Xanthine_DH_ssu. [Graphical view]
Gene3D	G3DSA:3.30.365.10. Ald_xan_DH_mo_bd. 2 hits. G3DSA:3.90.1170.50. Aldxan_DH_hamm. 1 hit. G3DSA:3.30.390.50. CO_DH_flav_C. 1 hit. G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF01315. Ald_Xan_dh_C. 1 hit. PF02738. Ald_Xan_dh_C2. 1 hit. PF03450. CO_deh_flav_C. 1 hit. PF00941. FAD_binding_5. 1 hit. PF00111. Fer2. 1 hit. PF01799. Fer2_2. 1 hit. [Graphical view]
PIRSF	PIRSF000127. Xanthine_DH. 1 hit.
ProDom	PD186071. 2Fe-2S_bind. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02963. xanthine_xdhA. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51387. FAD_PCMH. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XDH_CALVI

Accession

Primary (citable) accession number: P08793

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)