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P08793

- XDH_CALVI

UniProt

P08793 - XDH_CALVI

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Protein

Xanthine dehydrogenase

Gene

XDH

Organism
Calliphora vicina (Blue blowfly) (Calliphora erythrocephala)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid (By similarity).By similarity

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters.By similarity
  • FADBy similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur 1By similarity
Metal bindingi61 – 611Iron-sulfur 1By similarity
Metal bindingi64 – 641Iron-sulfur 1By similarity
Metal bindingi86 – 861Iron-sulfur 1By similarity
Metal bindingi126 – 1261Iron-sulfur 2By similarity
Metal bindingi129 – 1291Iron-sulfur 2By similarity
Metal bindingi161 – 1611Iron-sulfur 2By similarity
Metal bindingi163 – 1631Iron-sulfur 2By similarity
Binding sitei353 – 3531FADBy similarity
Binding sitei376 – 3761FADBy similarity
Binding sitei424 – 4241FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei442 – 4421FADBy similarity
Metal bindingi790 – 7901MolybdenumBy similarity
Metal bindingi821 – 8211Molybdenum; via carbonyl oxygenBy similarity
Binding sitei825 – 8251SubstrateBy similarity
Binding sitei903 – 9031SubstrateBy similarity
Metal bindingi935 – 9351Molybdenum; via amide nitrogenBy similarity
Binding sitei937 – 9371SubstrateBy similarity
Metal bindingi1102 – 11021Molybdenum; via amide nitrogenBy similarity
Active sitei1285 – 12851Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2808FADBy similarity
Nucleotide bindingi363 – 3675FADBy similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
  2. electron carrier activity Source: InterPro
  3. flavin adenine dinucleotide binding Source: UniProtKB
  4. iron ion binding Source: InterPro
  5. molybdopterin cofactor binding Source: UniProtKB
  6. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
  7. xanthine dehydrogenase activity Source: UniProtKB
  8. xanthine oxidase activity Source: InterPro

GO - Biological processi

  1. xanthine catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine dehydrogenase (EC:1.17.1.4)
Short name:
XD
Gene namesi
Name:XDH
OrganismiCalliphora vicina (Blue blowfly) (Calliphora erythrocephala)
Taxonomic identifieri7373 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaOestroideaCalliphoridaeCalliphorinaeCalliphora

Subcellular locationi

Peroxisome By similarity

GO - Cellular componenti

  1. peroxisome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13531353Xanthine dehydrogenasePRO_0000166078Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP08793.
SMRiP08793. Positions 15-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 104882Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini245 – 434190FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the xanthine dehydrogenase family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR022407. OxRdtase_Mopterin_BS.
IPR014307. Xanthine_DH_ssu.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08793-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQEHNAAVL DLNPTFSTLI FFVNGKKVID TNPDPECTLL TYLREKLRLC
60 70 80 90 100
GTKLGCGEGG CGACTVMISR IDTLTNRIKH IAVNACLTPV CAMHGSAVTT
110 120 130 140 150
VEGIGSTRTR LHPVQERLAK AHGSQCGFCT PGIVMSMYAL LRNLSQPSMK
160 170 180 190 200
DLEIAFQGNL CRCTGYRPIL EGYKTFTKEF GCAMGDKCCK VNGNKCGEGM
210 220 230 240 250
ENGGDMVDDK LFEKSEFVPF DPSQEPIFPP ELQLNKDWDS QTLVYKGERA
260 270 280 290 300
TWYRPGNLED LLKIKAQFPE AKLVVGNTEI GVEVKFKHFL YPVLVNPTKV
310 320 330 340 350
KEMIDVQELE DSIYFGASVS LMDIDRILRS SIEKLPEHQT RFFQCAVNML
360 370 380 390 400
HYFAGKQIRN VASLGGNIMT GSPISDMNPV LMAGAVKFKV AKYVEGQIKY
410 420 430 440 450
REVCMASGFF TGYRKNVIEP TEILVGLYFP KTLEHQYVVA FKQAKRRDDD
460 470 480 490 500
IAIVNAAINV FIDPRSITVD KVYMAFGGMA PTTVLATRTA DIMVKQQWNK
510 520 530 540 550
VLMERVVENL CAELPLAPSA PGGMIAYRRS LVVSLFFKAY LTITQQLIKS
560 570 580 590 600
GILPQDSLPQ EELSGSDVFH TPALKSAQLF EKVSNKQSEC DPIGRPKIHA
610 620 630 640 650
SALKQATGEA IYCDDMPRME NELYLALVLS TKAHAKILSI DASEALAMPG
660 670 680 690 700
VHAFFSSKDI TQHENEVGPV FHDEEVFASD MVYCQGQVIG AIAADNPNFS
710 720 730 740 750
SKTARKVTIE YEDIKPVIIT IEQAIEHKSY FPDYPRFTEI GDVEKAFSEA
760 770 780 790 800
DHVYEGSCRM GGQEHFYLET HASLAVPRDS DEIEIFCSTQ HPSEVQKLVA
810 820 830 840 850
HVLSTSAHRV VCRAKRLGGG FGGKESRAIA VALPVALACH RLRRPIRCML
860 870 880 890 900
DRDEDMMITG TRHPFLFKYK IAFTSEGRLT GCYIECYNNA GWSMDLSFSV
910 920 930 940 950
LERAMFHFEN CYKIPNIKVG GWVCKTNLPS NTAFRGFGGP QGMFAGEHII
960 970 980 990 1000
RDVARILGKD YLEIMKQNFY KEGDITHYQQ KLDNFPIEKC FYDCLQQSNY
1010 1020 1030 1040 1050
YQKRKEIEEF NRNHRWRKRG ISLVPTKYGI AFGVSHLNQA GALINIYADG
1060 1070 1080 1090 1100
SVLLSHGGVE IGQGLHTKMI QCCARALQIP IEFIHISETA TDKVPNTSPT
1110 1120 1130 1140 1150
AASSGSDLNG MAVLDACEKL NKRLAPIKEA NPNGSWTEWI NKAYFERVSL
1160 1170 1180 1190 1200
SATGFYRMPD IGYDPVQNPN ALMYNYFTNG VGSSIVEIDC LTGDHQVLST
1210 1220 1230 1240 1250
DIVMDIGSSL NPAIDIGQIE GAFMQGYGLF TLEEMIYSPQ GVLYSRGPGM
1260 1270 1280 1290 1300
YKLPGFADIP GEFNVTILTG AANPRAVYSS KAVGEPPLFI GCSVFFAIKE
1310 1320 1330 1340 1350
AITSARLMNG LSEDFKLESP ATSARIRMAC QDEFTNLIEQ PPAGSYVPWN

IVP
Length:1,353
Mass (Da):150,209
Last modified:November 1, 1988 - v1
Checksum:i7120361C57C3E297
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07229 mRNA. Translation: CAA30189.1.
X07323, X07324, X07325 Genomic DNA. Translation: CAA30281.1.
M18423 Genomic DNA. Translation: AAA27879.1.
PIRiJQ0407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07229 mRNA. Translation: CAA30189.1 .
X07323 , X07324 , X07325 Genomic DNA. Translation: CAA30281.1 .
M18423 Genomic DNA. Translation: AAA27879.1 .
PIRi JQ0407.

3D structure databases

ProteinModelPortali P08793.
SMRi P08793. Positions 15-178.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProi IPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR022407. OxRdtase_Mopterin_BS.
IPR014307. Xanthine_DH_ssu.
[Graphical view ]
Pfami PF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000127. Xanthine_DH. 1 hit.
SMARTi SM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view ]
SUPFAMi SSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsi TIGR02963. xanthine_xdhA. 1 hit.
PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Divergence of the nucleotide sequences encoding xanthine dehydrogenase in Calliphora vicina and Drosophila melanogaster."
    Houde M., Tiveron M.C., Bregegere F.
    Gene 85:391-402(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Cloning and partial characterization of the xanthine dehydrogenase gene of Calliphora vicina, a distant relative of Drosophila melanogaster."
    Rocher-Chambonnet C., Berreur P., Houde M., Tiveron M.C., Lepesant J.-A., Bregegere F.
    Gene 59:201-212(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 208-367.

Entry informationi

Entry nameiXDH_CALVI
AccessioniPrimary (citable) accession number: P08793
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3