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Protein

Keratin, type I cytoskeletal 16

Gene

KRT16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.By similarity

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin, I (acidic) and II (neutral to basic) (40-55 and 56-70 kDa, respectively).

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: UniProtKB

GO - Biological processi

  • aging Source: UniProtKB
  • cell proliferation Source: ProtInc
  • cornification Source: Reactome
  • cytoskeleton organization Source: UniProtKB
  • epidermis development Source: ProtInc
  • establishment of skin barrier Source: UniProtKB
  • hair cycle Source: UniProtKB
  • inflammatory response Source: UniProtKB
  • innate immune response Source: UniProtKB
  • intermediate filament cytoskeleton organization Source: Ensembl
  • keratinization Source: UniProtKB
  • keratinocyte differentiation Source: UniProtKB
  • keratinocyte migration Source: UniProtKB
  • morphogenesis of an epithelium Source: UniProtKB
  • negative regulation of cell migration Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-HSA-6805567. Keratinization.
R-HSA-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type I cytoskeletal 16
Alternative name(s):
Cytokeratin-16
Short name:
CK-16
Keratin-16
Short name:
K16
Gene namesi
Name:KRT16
Synonyms:KRT16A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:6423. KRT16.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • intermediate filament Source: UniProtKB
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Intermediate filament, Keratin

Pathology & Biotechi

Involvement in diseasei

Pachyonychia congenita 1 (PC1)12 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
See also OMIM:167200
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017065121M → T in PC1. 1 PublicationCorresponds to variant dbSNP:rs28928894Ensembl.1
Natural variantiVAR_012855122Q → P in PC1. 1 PublicationCorresponds to variant dbSNP:rs59349773Ensembl.1
Natural variantiVAR_072436124L → H in PC1. 1 PublicationCorresponds to variant dbSNP:rs58293603Ensembl.1
Natural variantiVAR_072437124L → P in PC1. 1 PublicationCorresponds to variant dbSNP:rs58293603Ensembl.1
Natural variantiVAR_013837124L → R in PC1. 1 PublicationCorresponds to variant dbSNP:rs58293603Ensembl.1
Natural variantiVAR_072438125N → D in PC1. 2 PublicationsCorresponds to variant dbSNP:rs58608173Ensembl.1
Natural variantiVAR_072439125N → G in PC1; requires 2 nucleotide substitutions. 1 PublicationCorresponds to variant dbSNP:rs587777717Ensembl.1
Natural variantiVAR_009183125N → S in FNEPPK1 and PC1. 5 PublicationsCorresponds to variant dbSNP:rs60723330Ensembl.1
Natural variantiVAR_009184127R → C in FNEPPK1 and PC1. 3 PublicationsCorresponds to variant dbSNP:rs59856285Ensembl.1
Natural variantiVAR_012856127R → P in PC1. 4 PublicationsCorresponds to variant dbSNP:rs57424749Ensembl.1
Natural variantiVAR_017066128L → Q in PC1. 2 PublicationsCorresponds to variant dbSNP:rs28928895Ensembl.1
Natural variantiVAR_035440130Missing in PC1. 1 Publication1
Natural variantiVAR_003846132L → P in PC1. 3 PublicationsCorresponds to variant dbSNP:rs60944949Ensembl.1
Natural variantiVAR_017067354K → N in PC1; late onset. 1 PublicationCorresponds to variant dbSNP:rs59328451Ensembl.1
Natural variantiVAR_072440421L → P in PC1. 1 Publication1
Keratoderma, palmoplantar, non-epidermolytic, focal 1 (FNEPPK1)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
See also OMIM:613000
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_009183125N → S in FNEPPK1 and PC1. 5 PublicationsCorresponds to variant dbSNP:rs60723330Ensembl.1
Natural variantiVAR_009184127R → C in FNEPPK1 and PC1. 3 PublicationsCorresponds to variant dbSNP:rs59856285Ensembl.1
KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.

Keywords - Diseasei

Disease mutation, Ectodermal dysplasia, Palmoplantar keratoderma

Organism-specific databases

DisGeNETi3868.
MalaCardsiKRT16.
MIMi167200. phenotype.
613000. phenotype.
OpenTargetsiENSG00000186832.
Orphaneti2199. Epidermolytic palmoplantar keratoderma.
2309. Pachyonychia congenita.
PharmGKBiPA30210.

Polymorphism and mutation databases

BioMutaiKRT16.
DMDMi23503075.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000636621 – 473Keratin, type I cytoskeletal 16Add BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55Omega-N-methylarginineBy similarity1
Modified residuei439PhosphoserineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP08779.
PeptideAtlasiP08779.
PRIDEiP08779.

PTM databases

iPTMnetiP08779.
PhosphoSitePlusiP08779.
SwissPalmiP08779.

Expressioni

Tissue specificityi

Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.

Gene expression databases

BgeeiENSG00000186832.
CleanExiHS_KRT16.
ExpressionAtlasiP08779. baseline and differential.
GenevisibleiP08779. HS.

Organism-specific databases

HPAiCAB000136.
HPA000539.

Interactioni

Subunit structurei

Heterodimer of a type I and a type II keratin. KRT16 associates with KRT6 isomers (KRT6A or KRT6B) (By similarity). Interacts with TCHP (PubMed:15731013). Interacts with TRADD (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi110066. 36 interactors.
IntActiP08779. 68 interactors.
MINTiMINT-1145021.
STRINGi9606.ENSP00000301653.

Structurei

3D structure databases

ProteinModelPortaliP08779.
SMRiP08779.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 116HeadAdd BLAST116
Regioni117 – 424RodAdd BLAST308
Regioni117 – 152Coil 1AAdd BLAST36
Regioni153 – 170Linker 1Add BLAST18
Regioni171 – 262Coil 1BAdd BLAST92
Regioni263 – 285Linker 12Add BLAST23
Regioni286 – 424Coil 2Add BLAST139
Regioni425 – 473TailAdd BLAST49

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFTF. Eukaryota.
ENOG410Y9IV. LUCA.
GeneTreeiENSGT00760000118808.
HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiP08779.
KOiK07604.
OMAiFEHELMM.
OrthoDBiEOG091G087I.
PhylomeDBiP08779.
TreeFamiTF332742.

Family and domain databases

InterProiView protein in InterPro
IPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiView protein in Pfam
PF00038. Filament. 1 hit.
PRINTSiPR01248. TYPE1KERATIN.
SMARTiView protein in SMART
SM01391. Filament. 1 hit.
PROSITEiView protein in PROSITE
PS00226. IF. 1 hit.

Sequencei

Sequence statusi: Complete.

P08779-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL
60 70 80 90 100
SVSSRFSSGG ACGLGGGYGG GFSSSSSFGS GFGGGYGGGL GAGFGGGLGA
110 120 130 140 150
GFGGGFAGGD GLLVGSEKVT MQNLNDRLAS YLDKVRALEE ANADLEVKIR
160 170 180 190 200
DWYQRQRPSE IKDYSPYFKT IEDLRNKIIA ATIENAQPIL QIDNARLAAD
210 220 230 240 250
DFRTKYEHEL ALRQTVEADV NGLRRVLDEL TLARTDLEMQ IEGLKEELAY
260 270 280 290 300
LRKNHEEEML ALRGQTGGDV NVEMDAAPGV DLSRILNEMR DQYEQMAEKN
310 320 330 340 350
RRDAETWFLS KTEELNKEVA SNSELVQSSR SEVTELRRVL QGLEIELQSQ
360 370 380 390 400
LSMKASLENS LEETKGRYCM QLSQIQGLIG SVEEQLAQLR CEMEQQSQEY
410 420 430 440 450
QILLDVKTRL EQEIATYRRL LEGEDAHLSS QQASGQSYSS REVFTSSSSS
460 470
SSRQTRPILK EQSSSSFSQG QSS
Length:473
Mass (Da):51,268
Last modified:January 23, 2007 - v4
Checksum:iBA8CE9F4716A88A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2T → A in AAB35421 (PubMed:7487986).Curated1
Sequence conflicti26G → A in AAA59460 (PubMed:2431270).Curated1
Sequence conflicti38G → A in AAA59460 (PubMed:2431270).Curated1
Sequence conflicti41 – 43RAP → PA in AAA59460 (PubMed:2431270).Curated3
Sequence conflicti49 – 50GL → A in AAA59460 (PubMed:2431270).Curated2
Sequence conflicti187 – 189QPI → HAL in AAA59460 (PubMed:2431270).Curated3
Sequence conflicti208 – 211HELA → ARTG in AAA59460 (PubMed:2431270).Curated4
Sequence conflicti352S → R in AAA59460 (PubMed:2431270).Curated1
Sequence conflicti452 – 460SRQTRPILK → AVRPGPSS in AAA59460 (PubMed:2431270).Curated9

Mass spectrometryi

Molecular mass is 50924.66 Da from positions 2 - 473. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_012854104 – 107Missing Rare variant; found as somatic mutation in a patient with localized epidermolytic hyperkeratosis in the right palm and the right sole; unknown pathological significance. 1 Publication4
Natural variantiVAR_017065121M → T in PC1. 1 PublicationCorresponds to variant dbSNP:rs28928894Ensembl.1
Natural variantiVAR_012855122Q → P in PC1. 1 PublicationCorresponds to variant dbSNP:rs59349773Ensembl.1
Natural variantiVAR_072436124L → H in PC1. 1 PublicationCorresponds to variant dbSNP:rs58293603Ensembl.1
Natural variantiVAR_072437124L → P in PC1. 1 PublicationCorresponds to variant dbSNP:rs58293603Ensembl.1
Natural variantiVAR_013837124L → R in PC1. 1 PublicationCorresponds to variant dbSNP:rs58293603Ensembl.1
Natural variantiVAR_072438125N → D in PC1. 2 PublicationsCorresponds to variant dbSNP:rs58608173Ensembl.1
Natural variantiVAR_072439125N → G in PC1; requires 2 nucleotide substitutions. 1 PublicationCorresponds to variant dbSNP:rs587777717Ensembl.1
Natural variantiVAR_009183125N → S in FNEPPK1 and PC1. 5 PublicationsCorresponds to variant dbSNP:rs60723330Ensembl.1
Natural variantiVAR_009184127R → C in FNEPPK1 and PC1. 3 PublicationsCorresponds to variant dbSNP:rs59856285Ensembl.1
Natural variantiVAR_012856127R → P in PC1. 4 PublicationsCorresponds to variant dbSNP:rs57424749Ensembl.1
Natural variantiVAR_017066128L → Q in PC1. 2 PublicationsCorresponds to variant dbSNP:rs28928895Ensembl.1
Natural variantiVAR_035440130Missing in PC1. 1 Publication1
Natural variantiVAR_003846132L → P in PC1. 3 PublicationsCorresponds to variant dbSNP:rs60944949Ensembl.1
Natural variantiVAR_017067354K → N in PC1; late onset. 1 PublicationCorresponds to variant dbSNP:rs59328451Ensembl.1
Natural variantiVAR_072440421L → P in PC1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28439
, M28432, M28433, M28434, M28435, M28436, M28437, M28438 Genomic DNA. Translation: AAA59460.1.
S79867 mRNA. Translation: AAB35421.1.
AF061809 Genomic DNA. Translation: AAD15829.1.
AF061812 mRNA. Translation: AAC99326.1.
AK290853 mRNA. Translation: BAF83542.1.
CH471152 Genomic DNA. Translation: EAW60749.1.
BC039169 mRNA. Translation: AAH39169.1.
S78514 Genomic DNA. Translation: AAB34564.1.
CCDSiCCDS11401.1.
PIRiA33652.
JC4313.
RefSeqiNP_005548.2. NM_005557.3.
UniGeneiHs.655160.

Genome annotation databases

EnsembliENST00000301653; ENSP00000301653; ENSG00000186832.
GeneIDi3868.
KEGGihsa:3868.
UCSCiuc002hxg.5. human.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiK1C16_HUMAN
AccessioniPrimary (citable) accession number: P08779
Secondary accession number(s): A8K488
, P30654, Q16402, Q9UBG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 179 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families