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P08773

- DCHM_BPT4

UniProt

P08773 - DCHM_BPT4

Protein

Deoxycytidylate 5-hydroxymethyltransferase

Gene

42

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei148 – 1481Sequence Analysis

    GO - Molecular functioni

    1. deoxycytidylate 5-hydroxymethyltransferase activity Source: UniProtKB-EC
    2. thymidylate synthase activity Source: InterPro

    GO - Biological processi

    1. dTMP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxycytidylate 5-hydroxymethyltransferase (EC:2.1.2.8)
    Short name:
    Deoxycytidylate hydroxymethylase
    Alternative name(s):
    dCMP hydroxymethylase
    Short name:
    dCMP HMase
    Gene namesi
    Name:42
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246Deoxycytidylate 5-hydroxymethyltransferasePRO_0000141068Add
    BLAST

    Structurei

    Secondary structure

    1
    246
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 2013
    Beta strandi28 – 303
    Beta strandi32 – 4211
    Beta strandi48 – 503
    Helixi54 – 6613
    Helixi71 – 733
    Beta strandi74 – 774
    Helixi80 – 856
    Helixi96 – 1016
    Helixi103 – 1053
    Helixi108 – 11811
    Beta strandi126 – 1283
    Helixi134 – 1374
    Helixi138 – 1425
    Beta strandi149 – 1579
    Beta strandi160 – 17112
    Helixi174 – 19724
    Beta strandi204 – 21815
    Helixi219 – 2213
    Helixi222 – 23110
    Turni238 – 2403

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B49X-ray2.30A/C1-246[»]
    1B5DX-ray2.20A/B1-246[»]
    1B5EX-ray1.60A/B1-241[»]
    ProteinModelPortaliP08773.
    SMRiP08773. Positions 1-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08773.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family.Curated

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    InterProiIPR014619. Deoxycytidylate_hydroxyMease.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036750. dCMP_HMase. 1 hit.
    SUPFAMiSSF55831. SSF55831. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P08773-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISDSMTVEE IRLHLGLALK EKDFVVDKTG VKTIEIIGAS FVADEPFIFG    50
    ALNDEYIQRE LEWYKSKSLF VKDIPGETPK IWQQVASSKG EINSNYGWAI 100
    WSEDNYAQYD MCLAELGQNP DSRRGIMIYT RPSMQFDYNK DGMSDFMCTN 150
    TVQYLIRDKK INAVVNMRSN DVVFGFRNDY AWQKYVLDKL VSDLNAGDST 200
    RQYKAGSIIW NVGSLHVYSR HFYLVDHWWK TGETHISKKD YVGKYA 246
    Length:246
    Mass (Da):28,489
    Last modified:November 1, 1988 - v1
    Checksum:i36F1C4EE23B51E8E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti164 – 1641V → L in AAA88467. (PubMed:2832395)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00148 Genomic DNA. Translation: CAA68342.1.
    AF158101 Genomic DNA. Translation: AAD42467.1.
    M22767 Genomic DNA. Translation: AAA88468.1.
    M22766 Genomic DNA. Translation: AAA88467.1.
    M37159 Genomic DNA. Translation: AAA21709.1.
    X03139 Genomic DNA. Translation: CAA26907.1.
    PIRiJS0786. SZBPT4.
    RefSeqiNP_049659.1. NC_000866.4.

    Genome annotation databases

    GeneIDi1258746.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00148 Genomic DNA. Translation: CAA68342.1 .
    AF158101 Genomic DNA. Translation: AAD42467.1 .
    M22767 Genomic DNA. Translation: AAA88468.1 .
    M22766 Genomic DNA. Translation: AAA88467.1 .
    M37159 Genomic DNA. Translation: AAA21709.1 .
    X03139 Genomic DNA. Translation: CAA26907.1 .
    PIRi JS0786. SZBPT4.
    RefSeqi NP_049659.1. NC_000866.4.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B49 X-ray 2.30 A/C 1-246 [» ]
    1B5D X-ray 2.20 A/B 1-246 [» ]
    1B5E X-ray 1.60 A/B 1-241 [» ]
    ProteinModelPortali P08773.
    SMRi P08773. Positions 1-246.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258746.

    Miscellaneous databases

    EvolutionaryTracei P08773.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    InterProi IPR014619. Deoxycytidylate_hydroxyMease.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036750. dCMP_HMase. 1 hit.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the deoxycytidylate hydroxymethylase gene of bacteriophage T4 (g42) and the homology of its gene product with thymidylate synthase of E. coli."
      Lamm N., Tomaschewski J., Rueger W.
      Nucleic Acids Res. 15:3920-3920(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: W.
    2. "Deoxycytidylate hydroxymethylase gene of bacteriophage T4. Nucleotide sequence determination and over-expression of the gene."
      Lamm N., Wang Y., Mathews C.K., Rueger W.
      Eur. J. Biochem. 172:553-563(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene."
      Thylen C.
      J. Bacteriol. 170:1994-1998(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-186 AND 225-246.
    5. "T4-induced alpha- and beta-glucosyltransferase: cloning of the genes and a comparison of their products based on sequencing data."
      Tomaschewski J., Gram H., Crabb J.W., Rueger W.
      Nucleic Acids Res. 13:7551-7568(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-246.

    Entry informationi

    Entry nameiDCHM_BPT4
    AccessioniPrimary (citable) accession number: P08773
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3