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Protein

Deoxycytidylate 5-hydroxymethyltransferase

Gene

42

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei148Sequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BRENDAi2.1.2.8. 732.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxycytidylate 5-hydroxymethyltransferase (EC:2.1.2.8)
Short name:
Deoxycytidylate hydroxymethylase
Alternative name(s):
dCMP hydroxymethylase
Short name:
dCMP HMase
Gene namesi
Name:42
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410681 – 246Deoxycytidylate 5-hydroxymethyltransferaseAdd BLAST246

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 20Combined sources13
Beta strandi28 – 30Combined sources3
Beta strandi32 – 42Combined sources11
Beta strandi48 – 50Combined sources3
Helixi54 – 66Combined sources13
Helixi71 – 73Combined sources3
Beta strandi74 – 77Combined sources4
Helixi80 – 85Combined sources6
Helixi96 – 101Combined sources6
Helixi103 – 105Combined sources3
Helixi108 – 118Combined sources11
Beta strandi126 – 128Combined sources3
Helixi134 – 137Combined sources4
Helixi138 – 142Combined sources5
Beta strandi149 – 157Combined sources9
Beta strandi160 – 171Combined sources12
Helixi174 – 197Combined sources24
Beta strandi204 – 218Combined sources15
Helixi219 – 221Combined sources3
Helixi222 – 231Combined sources10
Turni238 – 240Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B49X-ray2.30A/C1-246[»]
1B5DX-ray2.20A/B1-246[»]
1B5EX-ray1.60A/B1-241[»]
ProteinModelPortaliP08773.
SMRiP08773.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08773.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

KOiK19796.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
InterProiIPR014619. Deoxycytidylate_hydroxyMease.
IPR023451. Thymidate_synth/dCMP_Mease.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF036750. dCMP_HMase. 1 hit.
SUPFAMiSSF55831. SSF55831. 1 hit.

Sequencei

Sequence statusi: Complete.

P08773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISDSMTVEE IRLHLGLALK EKDFVVDKTG VKTIEIIGAS FVADEPFIFG
60 70 80 90 100
ALNDEYIQRE LEWYKSKSLF VKDIPGETPK IWQQVASSKG EINSNYGWAI
110 120 130 140 150
WSEDNYAQYD MCLAELGQNP DSRRGIMIYT RPSMQFDYNK DGMSDFMCTN
160 170 180 190 200
TVQYLIRDKK INAVVNMRSN DVVFGFRNDY AWQKYVLDKL VSDLNAGDST
210 220 230 240
RQYKAGSIIW NVGSLHVYSR HFYLVDHWWK TGETHISKKD YVGKYA
Length:246
Mass (Da):28,489
Last modified:November 1, 1988 - v1
Checksum:i36F1C4EE23B51E8E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164V → L in AAA88467 (PubMed:2832395).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00148 Genomic DNA. Translation: CAA68342.1.
AF158101 Genomic DNA. Translation: AAD42467.1.
M22767 Genomic DNA. Translation: AAA88468.1.
M22766 Genomic DNA. Translation: AAA88467.1.
M37159 Genomic DNA. Translation: AAA21709.1.
X03139 Genomic DNA. Translation: CAA26907.1.
PIRiJS0786. SZBPT4.
RefSeqiNP_049659.1. NC_000866.4.

Genome annotation databases

GeneIDi1258746.
KEGGivg:1258746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00148 Genomic DNA. Translation: CAA68342.1.
AF158101 Genomic DNA. Translation: AAD42467.1.
M22767 Genomic DNA. Translation: AAA88468.1.
M22766 Genomic DNA. Translation: AAA88467.1.
M37159 Genomic DNA. Translation: AAA21709.1.
X03139 Genomic DNA. Translation: CAA26907.1.
PIRiJS0786. SZBPT4.
RefSeqiNP_049659.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B49X-ray2.30A/C1-246[»]
1B5DX-ray2.20A/B1-246[»]
1B5EX-ray1.60A/B1-241[»]
ProteinModelPortaliP08773.
SMRiP08773.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258746.
KEGGivg:1258746.

Phylogenomic databases

KOiK19796.

Enzyme and pathway databases

BRENDAi2.1.2.8. 732.

Miscellaneous databases

EvolutionaryTraceiP08773.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
InterProiIPR014619. Deoxycytidylate_hydroxyMease.
IPR023451. Thymidate_synth/dCMP_Mease.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF036750. dCMP_HMase. 1 hit.
SUPFAMiSSF55831. SSF55831. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDCHM_BPT4
AccessioniPrimary (citable) accession number: P08773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.