P08773 (DCHM_BPT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxycytidylate 5-hydroxymethyltransferase
Short name=Deoxycytidylate hydroxymethylase
EC=2.1.2.8

Alternative name(s):
dCMP hydroxymethylase
Short name=dCMP HMase

Gene names

Name:

42

Organism

Enterobacteria phage T4 (Bacteriophage T4) [Reference proteome]

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Tevenvirinae › T4likevirus › Enterobacteria phage T4 sensu lato

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate.
Sequence similarities	Belongs to the thymidylate synthase family.

Ontologies

Keywords
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	deoxycytidylate 5-hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC thymidylate synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 246

246

Deoxycytidylate 5-hydroxymethyltransferase

PRO_0000141068

Sites

Active site

148

1

Potential

Experimental info

Sequence conflict

164

1

V → L in AAA88467. Ref.4

Secondary structure

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246

Helix Strand Turn

Details...

Helix

8 – 20

13

Beta strand

28 – 30

3

Beta strand

32 – 42

11

Beta strand

48 – 50

3

Helix

54 – 66

13

Helix

71 – 73

3

Beta strand

74 – 77

4

Helix

80 – 85

6

Helix

96 – 101

6

Helix

103 – 105

3

Helix

108 – 118

11

Beta strand

126 – 128

3

Helix

134 – 137

4

Helix

138 – 142

5

Beta strand

149 – 157

9

Beta strand

160 – 171

12

Helix

174 – 197

24

Beta strand

204 – 218

15

Helix

219 – 221

3

Helix

222 – 231

10

Turn

238 – 240

3

Sequences

	Sequence		Length	Mass (Da)
	P08773 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 36F1C4EE23B51E8E	FASTA	246	28,489
	10 20 30 40 50 60 MISDSMTVEE IRLHLGLALK EKDFVVDKTG VKTIEIIGAS FVADEPFIFG ALNDEYIQRE 70 80 90 100 110 120 LEWYKSKSLF VKDIPGETPK IWQQVASSKG EINSNYGWAI WSEDNYAQYD MCLAELGQNP 130 140 150 160 170 180 DSRRGIMIYT RPSMQFDYNK DGMSDFMCTN TVQYLIRDKK INAVVNMRSN DVVFGFRNDY 190 200 210 220 230 240 AWQKYVLDKL VSDLNAGDST RQYKAGSIIW NVGSLHVYSR HFYLVDHWWK TGETHISKKD YVGKYA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the deoxycytidylate hydroxymethylase gene of bacteriophage T4 (g42) and the homology of its gene product with thymidylate synthase of E. coli." Lamm N., Tomaschewski J., Rueger W. Nucleic Acids Res. 15:3920-3920(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W.
[2]	"Deoxycytidylate hydroxymethylase gene of bacteriophage T4. Nucleotide sequence determination and over-expression of the gene." Lamm N., Wang Y., Mathews C.K., Rueger W. Eur. J. Biochem. 172:553-563(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene." Thylen C. J. Bacteriol. 170:1994-1998(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-186 AND 225-246.
[5]	"T4-induced alpha- and beta-glucosyltransferase: cloning of the genes and a comparison of their products based on sequencing data." Tomaschewski J., Gram H., Crabb J.W., Rueger W. Nucleic Acids Res. 13:7551-7568(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-246.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y00148 Genomic DNA. Translation: CAA68342.1.
AF158101 Genomic DNA. Translation: AAD42467.1.
M22767 Genomic DNA. Translation: AAA88468.1.
M22766 Genomic DNA. Translation: AAA88467.1.
M37159 Genomic DNA. Translation: AAA21709.1.
X03139 Genomic DNA. Translation: CAA26907.1.

PIR

SZBPT4. JS0786.

RefSeq

NP_049659.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B49	X-ray	2.30	A/C	1-246	[»]
1B5D	X-ray	2.20	A/B	1-246	[»]
1B5E	X-ray	1.60	A/B	1-241	[»]

ProteinModelPortal

P08773.

SMR

P08773. Positions 1-246.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1258746.

Phylogenomic databases

ProtClustDB

CLSP2343284.

Family and domain databases

Gene3D

3.30.572.10. 1 hit.

InterPro

IPR014619. Deoxycytidylate_hydroxyMease.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]

Pfam

PF00303. Thymidylat_synt. 1 hit.
[Graphical view]

PIRSF

PIRSF036750. dCMP_HMase. 1 hit.

SUPFAM

SSF55831. Thymidylat_synth_C. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P08773.

Entry information

Entry name

DCHM_BPT4

Accession

Primary (citable) accession number: P08773

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program