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P08773

- DCHM_BPT4

UniProt

P08773 - DCHM_BPT4

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Protein

Deoxycytidylate 5-hydroxymethyltransferase

Gene

42

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei148 – 1481Sequence Analysis

GO - Molecular functioni

  1. deoxycytidylate 5-hydroxymethyltransferase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: InterPro

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxycytidylate 5-hydroxymethyltransferase (EC:2.1.2.8)
Short name:
Deoxycytidylate hydroxymethylase
Alternative name(s):
dCMP hydroxymethylase
Short name:
dCMP HMase
Gene namesi
Name:42
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246Deoxycytidylate 5-hydroxymethyltransferasePRO_0000141068Add
BLAST

Structurei

Secondary structure

1
246
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2013
Beta strandi28 – 303
Beta strandi32 – 4211
Beta strandi48 – 503
Helixi54 – 6613
Helixi71 – 733
Beta strandi74 – 774
Helixi80 – 856
Helixi96 – 1016
Helixi103 – 1053
Helixi108 – 11811
Beta strandi126 – 1283
Helixi134 – 1374
Helixi138 – 1425
Beta strandi149 – 1579
Beta strandi160 – 17112
Helixi174 – 19724
Beta strandi204 – 21815
Helixi219 – 2213
Helixi222 – 23110
Turni238 – 2403

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B49X-ray2.30A/C1-246[»]
1B5DX-ray2.20A/B1-246[»]
1B5EX-ray1.60A/B1-241[»]
ProteinModelPortaliP08773.
SMRiP08773. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08773.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
InterProiIPR014619. Deoxycytidylate_hydroxyMease.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF036750. dCMP_HMase. 1 hit.
SUPFAMiSSF55831. SSF55831. 1 hit.

Sequencei

Sequence statusi: Complete.

P08773 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISDSMTVEE IRLHLGLALK EKDFVVDKTG VKTIEIIGAS FVADEPFIFG
60 70 80 90 100
ALNDEYIQRE LEWYKSKSLF VKDIPGETPK IWQQVASSKG EINSNYGWAI
110 120 130 140 150
WSEDNYAQYD MCLAELGQNP DSRRGIMIYT RPSMQFDYNK DGMSDFMCTN
160 170 180 190 200
TVQYLIRDKK INAVVNMRSN DVVFGFRNDY AWQKYVLDKL VSDLNAGDST
210 220 230 240
RQYKAGSIIW NVGSLHVYSR HFYLVDHWWK TGETHISKKD YVGKYA
Length:246
Mass (Da):28,489
Last modified:November 1, 1988 - v1
Checksum:i36F1C4EE23B51E8E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641V → L in AAA88467. (PubMed:2832395)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00148 Genomic DNA. Translation: CAA68342.1.
AF158101 Genomic DNA. Translation: AAD42467.1.
M22767 Genomic DNA. Translation: AAA88468.1.
M22766 Genomic DNA. Translation: AAA88467.1.
M37159 Genomic DNA. Translation: AAA21709.1.
X03139 Genomic DNA. Translation: CAA26907.1.
PIRiJS0786. SZBPT4.
RefSeqiNP_049659.1. NC_000866.4.

Genome annotation databases

GeneIDi1258746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00148 Genomic DNA. Translation: CAA68342.1 .
AF158101 Genomic DNA. Translation: AAD42467.1 .
M22767 Genomic DNA. Translation: AAA88468.1 .
M22766 Genomic DNA. Translation: AAA88467.1 .
M37159 Genomic DNA. Translation: AAA21709.1 .
X03139 Genomic DNA. Translation: CAA26907.1 .
PIRi JS0786. SZBPT4.
RefSeqi NP_049659.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B49 X-ray 2.30 A/C 1-246 [» ]
1B5D X-ray 2.20 A/B 1-246 [» ]
1B5E X-ray 1.60 A/B 1-241 [» ]
ProteinModelPortali P08773.
SMRi P08773. Positions 1-246.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1258746.

Miscellaneous databases

EvolutionaryTracei P08773.

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
InterProi IPR014619. Deoxycytidylate_hydroxyMease.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view ]
Pfami PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF036750. dCMP_HMase. 1 hit.
SUPFAMi SSF55831. SSF55831. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the deoxycytidylate hydroxymethylase gene of bacteriophage T4 (g42) and the homology of its gene product with thymidylate synthase of E. coli."
    Lamm N., Tomaschewski J., Rueger W.
    Nucleic Acids Res. 15:3920-3920(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W.
  2. "Deoxycytidylate hydroxymethylase gene of bacteriophage T4. Nucleotide sequence determination and over-expression of the gene."
    Lamm N., Wang Y., Mathews C.K., Rueger W.
    Eur. J. Biochem. 172:553-563(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene."
    Thylen C.
    J. Bacteriol. 170:1994-1998(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-186 AND 225-246.
  5. "T4-induced alpha- and beta-glucosyltransferase: cloning of the genes and a comparison of their products based on sequencing data."
    Tomaschewski J., Gram H., Crabb J.W., Rueger W.
    Nucleic Acids Res. 13:7551-7568(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-246.

Entry informationi

Entry nameiDCHM_BPT4
AccessioniPrimary (citable) accession number: P08773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: October 1, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3