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P08773 (DCHM_BPT4) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxycytidylate 5-hydroxymethyltransferase

Short name=Deoxycytidylate hydroxymethylase
EC=2.1.2.8
Alternative name(s):
dCMP hydroxymethylase
Short name=dCMP HMase
Gene names
Name:42
OrganismEnterobacteria phage T4 (Bacteriophage T4) [Reference proteome]
Taxonomic identifier10665 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate.

Sequence similarities

Belongs to the thymidylate synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246Deoxycytidylate 5-hydroxymethyltransferase
PRO_0000141068

Sites

Active site1481 Potential

Experimental info

Sequence conflict1641V → L in AAA88467. Ref.4

Secondary structure

....................................... 246
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08773 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 36F1C4EE23B51E8E

FASTA24628,489
        10         20         30         40         50         60 
MISDSMTVEE IRLHLGLALK EKDFVVDKTG VKTIEIIGAS FVADEPFIFG ALNDEYIQRE 

        70         80         90        100        110        120 
LEWYKSKSLF VKDIPGETPK IWQQVASSKG EINSNYGWAI WSEDNYAQYD MCLAELGQNP 

       130        140        150        160        170        180 
DSRRGIMIYT RPSMQFDYNK DGMSDFMCTN TVQYLIRDKK INAVVNMRSN DVVFGFRNDY 

       190        200        210        220        230        240 
AWQKYVLDKL VSDLNAGDST RQYKAGSIIW NVGSLHVYSR HFYLVDHWWK TGETHISKKD 


YVGKYA 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the deoxycytidylate hydroxymethylase gene of bacteriophage T4 (g42) and the homology of its gene product with thymidylate synthase of E. coli."
Lamm N., Tomaschewski J., Rueger W.
Nucleic Acids Res. 15:3920-3920(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: W.
[2]"Deoxycytidylate hydroxymethylase gene of bacteriophage T4. Nucleotide sequence determination and over-expression of the gene."
Lamm N., Wang Y., Mathews C.K., Rueger W.
Eur. J. Biochem. 172:553-563(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Bacteriophage T4 genome."
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.
Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene."
Thylen C.
J. Bacteriol. 170:1994-1998(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-186 AND 225-246.
[5]"T4-induced alpha- and beta-glucosyltransferase: cloning of the genes and a comparison of their products based on sequencing data."
Tomaschewski J., Gram H., Crabb J.W., Rueger W.
Nucleic Acids Res. 13:7551-7568(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-246.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00148 Genomic DNA. Translation: CAA68342.1.
AF158101 Genomic DNA. Translation: AAD42467.1.
M22767 Genomic DNA. Translation: AAA88468.1.
M22766 Genomic DNA. Translation: AAA88467.1.
M37159 Genomic DNA. Translation: AAA21709.1.
X03139 Genomic DNA. Translation: CAA26907.1.
PIRSZBPT4. JS0786.
RefSeqNP_049659.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B49X-ray2.30A/C1-246[»]
1B5DX-ray2.20A/B1-246[»]
1B5EX-ray1.60A/B1-241[»]
ProteinModelPortalP08773.
SMRP08773. Positions 1-246.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1258746.

Phylogenomic databases

ProtClustDBCLSP2343284.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
InterProIPR014619. Deoxycytidylate_hydroxyMease.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFPIRSF036750. dCMP_HMase. 1 hit.
SUPFAMSSF55831. SSF55831. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP08773.

Entry information

Entry nameDCHM_BPT4
AccessionPrimary (citable) accession number: P08773
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references