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P08764

- T3RE_BPP1

UniProt

P08764 - T3RE_BPP1

Protein

Type III restriction-modification system EcoPI enzyme res

Gene

res

Organism
Enterobacteria phage P1 (Bacteriophage P1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    This protein cuts the DNA some 25 base-pairs to the 3'-end of the recognition site. It is only required for restriction but needs the presence of the modification enzyme.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: InterPro
    3. helicase activity Source: UniProtKB-KW
    4. Type III site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Protein family/group databases

    REBASEi988. EcoPI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type III restriction-modification system EcoPI enzyme res (EC:3.1.21.5)
    Gene namesi
    Name:res
    OrganismiEnterobacteria phage P1 (Bacteriophage P1)
    Taxonomic identifieri10678 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePunalikevirus
    Virus hostiEnterobacteriaceae [TaxID: 543]
    ProteomesiUP000008091: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 970970Type III restriction-modification system EcoPI enzyme resPRO_0000077374Add
    BLAST

    Interactioni

    Subunit structurei

    Contains two different subunits: res and mod.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR006935. Helicase/UvrB_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF04851. ResIII. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P08764-1 [UniParc]FASTAAdd to Basket

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    MSKGFTFEKN LPHQKAGVDA VMNVFVSATS HQEDNVSIRL LVNPELRLTE    50
    QQYYKNIKKV QELNGIEHVK NNYDARSNVI DVSMETGTGK TYTYTKTIFD 100
    LNKSFGINKF IIIVPTLSIK AGTVNFLKSD ALKEHFRDDY EREIKTYVVE 150
    SQKNAGKSTK SYMPQAIHDF VEASNFNKKY IHVLVINTGM IHSKNLNSTY 200
    DVGLLDNHFD SPFSALGAVK PFIIIDEPHK FPTGKKTWEN IEKFNAQYII 250
    RYGATFSEGY KNLVYRLTAV DAFNEDLVKG IDAYIEDIVG DGDANLKFIK 300
    SDGEEVTFEL NENNKKTLFK LTKGESLSKT HSAIHDLTLD ALGKNTVVLS 350
    NGIELKIGCS INPYSYDQTL ADSMMRKAIK EHFKLEKEFL TQRPRIKPLT 400
    LFFIDDIEGY RDGNNIAGSL KAKFEEYVLA EANELLKIEK DEFYSNYLEK 450
    TVKDISSVHG GYFSKDNSDK DDKIEKEINE ILHDKELLLS LDNPRRFIFS 500
    KWTLREGWDN PNVFQICKLR SSGSTTSKLQ EVGRGLRLPV NEYMCRVKDR 550
    NFTLKYYVDF TEKDFVDSLV KEVNESSFKE RVPSKFTQEL KEQIRAQYPE 600
    LSSRALMNEL FNDEIIDDND NFKDSDAYSR LKSKYPAAFP IGVKPGKIKK 650
    ATDGKRRTKM RVGKFSELKE LWELINQKAV IEYKINSENE FLSIFKSFML 700
    EETERFTKSG VHTRIDKIYI HNDMAMSKSI VSDDDDFAKL NTMSYREFLD 750
    NLSQTIFVKH DTLHKVFCDI KDTINITEYL NIQTIRKIKS GFSKYLLNNS 800
    FNKFSLGYNL ISGSIHPTKF TNADGKPLDE VLSSDLGVLQ DNSKAPLDTY 850
    LFEEVFYDSE LERRNITDRE IQSVVVFSKI PKNSIKIPVA GGYTYSPDFA 900
    YVVKTAEGDY LNFIIETKNV DSKDSLRLEE KKKIEHAQAL FNQISQSVKV 950
    EFKTQFANDD IYQLIKSALP 970
    Length:970
    Mass (Da):111,459
    Last modified:November 1, 1988 - v1
    Checksum:iB599110154D723AA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06287 Genomic DNA. Translation: CAA29615.1.
    AF234172 Genomic DNA. Translation: AAQ13982.1.
    PIRiS01352.
    RefSeqiYP_006476.1. NC_005856.1.

    Genome annotation databases

    GeneIDi2777489.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06287 Genomic DNA. Translation: CAA29615.1 .
    AF234172 Genomic DNA. Translation: AAQ13982.1 .
    PIRi S01352.
    RefSeqi YP_006476.1. NC_005856.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 988. EcoPI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2777489.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR006935. Helicase/UvrB_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF04851. ResIII. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants."
      Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A.
      J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiT3RE_BPP1
    AccessioniPrimary (citable) accession number: P08764
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3