Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P08764

- T3RE_BPP1

UniProt

P08764 - T3RE_BPP1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Type III restriction-modification system EcoPI enzyme res

Gene

res

Organism
Enterobacteria phage P1 (Bacteriophage P1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This protein cuts the DNA some 25 base-pairs to the 3'-end of the recognition site. It is only required for restriction but needs the presence of the modification enzyme.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. helicase activity Source: UniProtKB-KW
  4. Type III site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

REBASEi988. EcoPI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type III restriction-modification system EcoPI enzyme res (EC:3.1.21.5)
Gene namesi
Name:res
OrganismiEnterobacteria phage P1 (Bacteriophage P1)
Taxonomic identifieri10678 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePunalikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]
ProteomesiUP000008091: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 970970Type III restriction-modification system EcoPI enzyme resPRO_0000077374Add
BLAST

Interactioni

Subunit structurei

Contains two different subunits: res and mod.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006935. Helicase/UvrB_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04851. ResIII. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequencei

Sequence statusi: Complete.

P08764-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKGFTFEKN LPHQKAGVDA VMNVFVSATS HQEDNVSIRL LVNPELRLTE
60 70 80 90 100
QQYYKNIKKV QELNGIEHVK NNYDARSNVI DVSMETGTGK TYTYTKTIFD
110 120 130 140 150
LNKSFGINKF IIIVPTLSIK AGTVNFLKSD ALKEHFRDDY EREIKTYVVE
160 170 180 190 200
SQKNAGKSTK SYMPQAIHDF VEASNFNKKY IHVLVINTGM IHSKNLNSTY
210 220 230 240 250
DVGLLDNHFD SPFSALGAVK PFIIIDEPHK FPTGKKTWEN IEKFNAQYII
260 270 280 290 300
RYGATFSEGY KNLVYRLTAV DAFNEDLVKG IDAYIEDIVG DGDANLKFIK
310 320 330 340 350
SDGEEVTFEL NENNKKTLFK LTKGESLSKT HSAIHDLTLD ALGKNTVVLS
360 370 380 390 400
NGIELKIGCS INPYSYDQTL ADSMMRKAIK EHFKLEKEFL TQRPRIKPLT
410 420 430 440 450
LFFIDDIEGY RDGNNIAGSL KAKFEEYVLA EANELLKIEK DEFYSNYLEK
460 470 480 490 500
TVKDISSVHG GYFSKDNSDK DDKIEKEINE ILHDKELLLS LDNPRRFIFS
510 520 530 540 550
KWTLREGWDN PNVFQICKLR SSGSTTSKLQ EVGRGLRLPV NEYMCRVKDR
560 570 580 590 600
NFTLKYYVDF TEKDFVDSLV KEVNESSFKE RVPSKFTQEL KEQIRAQYPE
610 620 630 640 650
LSSRALMNEL FNDEIIDDND NFKDSDAYSR LKSKYPAAFP IGVKPGKIKK
660 670 680 690 700
ATDGKRRTKM RVGKFSELKE LWELINQKAV IEYKINSENE FLSIFKSFML
710 720 730 740 750
EETERFTKSG VHTRIDKIYI HNDMAMSKSI VSDDDDFAKL NTMSYREFLD
760 770 780 790 800
NLSQTIFVKH DTLHKVFCDI KDTINITEYL NIQTIRKIKS GFSKYLLNNS
810 820 830 840 850
FNKFSLGYNL ISGSIHPTKF TNADGKPLDE VLSSDLGVLQ DNSKAPLDTY
860 870 880 890 900
LFEEVFYDSE LERRNITDRE IQSVVVFSKI PKNSIKIPVA GGYTYSPDFA
910 920 930 940 950
YVVKTAEGDY LNFIIETKNV DSKDSLRLEE KKKIEHAQAL FNQISQSVKV
960 970
EFKTQFANDD IYQLIKSALP
Length:970
Mass (Da):111,459
Last modified:November 1, 1988 - v1
Checksum:iB599110154D723AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06287 Genomic DNA. Translation: CAA29615.1.
AF234172 Genomic DNA. Translation: AAQ13982.1.
PIRiS01352.
RefSeqiYP_006476.1. NC_005856.1.

Genome annotation databases

GeneIDi2777489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06287 Genomic DNA. Translation: CAA29615.1 .
AF234172 Genomic DNA. Translation: AAQ13982.1 .
PIRi S01352.
RefSeqi YP_006476.1. NC_005856.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 988. EcoPI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2777489.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR006935. Helicase/UvrB_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF04851. ResIII. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants."
    Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A.
    J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiT3RE_BPP1
AccessioniPrimary (citable) accession number: P08764
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3