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P08764 (T3RE_BPP1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type III restriction-modification system EcoPI enzyme res

EC=3.1.21.5
Gene names
Name:res
OrganismEnterobacteria phage P1 (Bacteriophage P1) [Reference proteome]
Taxonomic identifier10678 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePunalikevirus
Virus hostEnterobacteriaceae [TaxID: 543]

Protein attributes

Sequence length970 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This protein cuts the DNA some 25 base-pairs to the 3'-end of the recognition site. It is only required for restriction but needs the presence of the modification enzyme.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Subunit structure

Contains two different subunits: res and mod.

Sequence similarities

Belongs to the type III restriction-modification system res protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 970970Type III restriction-modification system EcoPI enzyme res
PRO_0000077374

Sequences

Sequence LengthMass (Da)Tools
P08764 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: B599110154D723AA

FASTA970111,459
        10         20         30         40         50         60 
MSKGFTFEKN LPHQKAGVDA VMNVFVSATS HQEDNVSIRL LVNPELRLTE QQYYKNIKKV 

        70         80         90        100        110        120 
QELNGIEHVK NNYDARSNVI DVSMETGTGK TYTYTKTIFD LNKSFGINKF IIIVPTLSIK 

       130        140        150        160        170        180 
AGTVNFLKSD ALKEHFRDDY EREIKTYVVE SQKNAGKSTK SYMPQAIHDF VEASNFNKKY 

       190        200        210        220        230        240 
IHVLVINTGM IHSKNLNSTY DVGLLDNHFD SPFSALGAVK PFIIIDEPHK FPTGKKTWEN 

       250        260        270        280        290        300 
IEKFNAQYII RYGATFSEGY KNLVYRLTAV DAFNEDLVKG IDAYIEDIVG DGDANLKFIK 

       310        320        330        340        350        360 
SDGEEVTFEL NENNKKTLFK LTKGESLSKT HSAIHDLTLD ALGKNTVVLS NGIELKIGCS 

       370        380        390        400        410        420 
INPYSYDQTL ADSMMRKAIK EHFKLEKEFL TQRPRIKPLT LFFIDDIEGY RDGNNIAGSL 

       430        440        450        460        470        480 
KAKFEEYVLA EANELLKIEK DEFYSNYLEK TVKDISSVHG GYFSKDNSDK DDKIEKEINE 

       490        500        510        520        530        540 
ILHDKELLLS LDNPRRFIFS KWTLREGWDN PNVFQICKLR SSGSTTSKLQ EVGRGLRLPV 

       550        560        570        580        590        600 
NEYMCRVKDR NFTLKYYVDF TEKDFVDSLV KEVNESSFKE RVPSKFTQEL KEQIRAQYPE 

       610        620        630        640        650        660 
LSSRALMNEL FNDEIIDDND NFKDSDAYSR LKSKYPAAFP IGVKPGKIKK ATDGKRRTKM 

       670        680        690        700        710        720 
RVGKFSELKE LWELINQKAV IEYKINSENE FLSIFKSFML EETERFTKSG VHTRIDKIYI 

       730        740        750        760        770        780 
HNDMAMSKSI VSDDDDFAKL NTMSYREFLD NLSQTIFVKH DTLHKVFCDI KDTINITEYL 

       790        800        810        820        830        840 
NIQTIRKIKS GFSKYLLNNS FNKFSLGYNL ISGSIHPTKF TNADGKPLDE VLSSDLGVLQ 

       850        860        870        880        890        900 
DNSKAPLDTY LFEEVFYDSE LERRNITDRE IQSVVVFSKI PKNSIKIPVA GGYTYSPDFA 

       910        920        930        940        950        960 
YVVKTAEGDY LNFIIETKNV DSKDSLRLEE KKKIEHAQAL FNQISQSVKV EFKTQFANDD 

       970 
IYQLIKSALP 

« Hide

References

« Hide 'large scale' references
[1]"Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants."
Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A.
J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genome of bacteriophage P1."
Lobocka M.B., Rose D.J., Plunkett G. III, Rusin M., Samojedny A., Lehnherr H., Yarmolinsky M.B., Blattner F.R.
J. Bacteriol. 186:7032-7068(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06287 Genomic DNA. Translation: CAA29615.1.
AF234172 Genomic DNA. Translation: AAQ13982.1.
PIRS01352.
RefSeqYP_006476.1. NC_005856.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE988. EcoPI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2777489.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR006935. Helicase/UvrB_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF04851. ResIII. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
ProtoNetSearch...

Entry information

Entry nameT3RE_BPP1
AccessionPrimary (citable) accession number: P08764
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries