P08764 (T3RE_BPP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type III restriction-modification system EcoPI enzyme res
EC=3.1.21.5

Gene names

Name:

res

Organism

Enterobacteria phage P1 (Bacteriophage P1) [Reference proteome]

Taxonomic identifier

10678 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Punalikevirus

Virus host

Enterobacteriaceae [TaxID: 543]

Protein attributes

Sequence length	970 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein cuts the DNA some 25 base-pairs to the 3'-end of the recognition site. It is only required for restriction but needs the presence of the modification enzyme.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.
Subunit structure	Contains two different subunits: res and mod.
Sequence similarities	Belongs to the type III restriction-modification system res protein family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro Type III site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC helicase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 970	970	Type III restriction-modification system EcoPI enzyme res		PRO_0000077374

Sequences

	Sequence		Length	Mass (Da)
	P08764 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: B599110154D723AA	FASTA	970	111,459
	10 20 30 40 50 60 MSKGFTFEKN LPHQKAGVDA VMNVFVSATS HQEDNVSIRL LVNPELRLTE QQYYKNIKKV 70 80 90 100 110 120 QELNGIEHVK NNYDARSNVI DVSMETGTGK TYTYTKTIFD LNKSFGINKF IIIVPTLSIK 130 140 150 160 170 180 AGTVNFLKSD ALKEHFRDDY EREIKTYVVE SQKNAGKSTK SYMPQAIHDF VEASNFNKKY 190 200 210 220 230 240 IHVLVINTGM IHSKNLNSTY DVGLLDNHFD SPFSALGAVK PFIIIDEPHK FPTGKKTWEN 250 260 270 280 290 300 IEKFNAQYII RYGATFSEGY KNLVYRLTAV DAFNEDLVKG IDAYIEDIVG DGDANLKFIK 310 320 330 340 350 360 SDGEEVTFEL NENNKKTLFK LTKGESLSKT HSAIHDLTLD ALGKNTVVLS NGIELKIGCS 370 380 390 400 410 420 INPYSYDQTL ADSMMRKAIK EHFKLEKEFL TQRPRIKPLT LFFIDDIEGY RDGNNIAGSL 430 440 450 460 470 480 KAKFEEYVLA EANELLKIEK DEFYSNYLEK TVKDISSVHG GYFSKDNSDK DDKIEKEINE 490 500 510 520 530 540 ILHDKELLLS LDNPRRFIFS KWTLREGWDN PNVFQICKLR SSGSTTSKLQ EVGRGLRLPV 550 560 570 580 590 600 NEYMCRVKDR NFTLKYYVDF TEKDFVDSLV KEVNESSFKE RVPSKFTQEL KEQIRAQYPE 610 620 630 640 650 660 LSSRALMNEL FNDEIIDDND NFKDSDAYSR LKSKYPAAFP IGVKPGKIKK ATDGKRRTKM 670 680 690 700 710 720 RVGKFSELKE LWELINQKAV IEYKINSENE FLSIFKSFML EETERFTKSG VHTRIDKIYI 730 740 750 760 770 780 HNDMAMSKSI VSDDDDFAKL NTMSYREFLD NLSQTIFVKH DTLHKVFCDI KDTINITEYL 790 800 810 820 830 840 NIQTIRKIKS GFSKYLLNNS FNKFSLGYNL ISGSIHPTKF TNADGKPLDE VLSSDLGVLQ 850 860 870 880 890 900 DNSKAPLDTY LFEEVFYDSE LERRNITDRE IQSVVVFSKI PKNSIKIPVA GGYTYSPDFA 910 920 930 940 950 960 YVVKTAEGDY LNFIIETKNV DSKDSLRLEE KKKIEHAQAL FNQISQSVKV EFKTQFANDD 970 IYQLIKSALP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants." Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A. J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome of bacteriophage P1." Lobocka M.B., Rose D.J., Plunkett G. III, Rusin M., Samojedny A., Lehnherr H., Yarmolinsky M.B., Blattner F.R. J. Bacteriol. 186:7032-7068(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06287 Genomic DNA. Translation: CAA29615.1. AF234172 Genomic DNA. Translation: AAQ13982.1.
PIR	S01352.
RefSeq	YP_006476.1. NC_005856.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	988. EcoPI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2777489.
Family and domain databases
InterPro	IPR006935. Helicase/UvrB_dom. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF04851. ResIII. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 2 hits.
ProtoNet	Search...

Entry information

Entry name

T3RE_BPP1

Accession

Primary (citable) accession number: P08764

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	May 29, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program