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P08763 (T3MO_BPP1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type III restriction-modification system EcoPI enzyme mod

Short name=M.EcoPI
EC=2.1.1.72
Alternative name(s):
EcoPI methyltransferase
Gene names
Name:MOD
OrganismEnterobacteria phage P1 (Bacteriophage P1)
Taxonomic identifier10678 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePunalikevirus
Virus hostEnterobacteriaceae [TaxID: 543]

Protein attributes

Sequence length646 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds the system-specific DNA recognition site 5'-AGACC-3'. Necessary for restriction and for methylation of A-3.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Subunit structure

Contains two different subunits: res and mod. Mod is a homotetramer By similarity.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 646646Type III restriction-modification system EcoPI enzyme mod
PRO_0000088030

Regions

Region123 – 1264Binding of S-adenosyl methionine Potential

Sequences

Sequence LengthMass (Da)Tools
P08763 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: DB2D8724777A5412

FASTA64673,486
        10         20         30         40         50         60 
MKKETIFSEV ETANSKQLAV LKANFPQCFD KNGAFIQEKL LEIIRASEVE LSKESYSLNW 

        70         80         90        100        110        120 
LGKSYARLLA NLPPKTLLAE DKTHNQQEEN KNSQNLLIKG DNLEVLKHMV NAYAEKVNMI 

       130        140        150        160        170        180 
YIDPPYNTGK DGFVYNDDRK FTPEQLSELA GIELDEANRI LEFTTKGSSS HSAWLTFIYP 

       190        200        210        220        230        240 
RLYIARELLK EDGVIFISID DNEDKQLGLL CDEVFGQGNF VAKLPTIMNL KGNHDNFGFS 

       250        260        270        280        290        300 
DTHEYIYVYA KNKDVCSLGQ FDIDESEVEK EWDEDEYGLF KRADTLKRTG QDASRKSRPK 

       310        320        330        340        350        360 
GWFPVFINSE NKVYVTDDDK PLNEDDYVLY PVSPTGEELS WSWGKKKIND EFYNLIVIDI 

       370        380        390        400        410        420 
KDGKNIYKKQ RPALGELPTK KPKSIWYKPE YSTSTATTEL KNLLGAKLFE GPKPVPLITD 

       430        440        450        460        470        480 
LVKIGTKKDS LVLDFFAGSG TTAEAVAYLN EKDSGCRNFI CIQKDEVINK TKNAYSLGYR 

       490        500        510        520        530        540 
SIFEITKKRI QEVFKKSTTT SDNAAKIGFK VIHTIDDFRA KVESELTLTN HTFFDDAVLT 

       550        560        570        580        590        600 
PEQYDALLTT WCVYDGSLLT TPIEDVDLSG YTAHFCNGRL YLIAPNFTSE ALKALLQKLD 

       610        620        630        640 
SDEDFAPNKV VFYGCNFESA KQRELNEALK SYANKKSIEL DLVVRN 

« Hide

References

[1]"Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants."
Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A.
J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06287 Genomic DNA. Translation: CAA29614.1.
PIRS01351.

3D structure databases

ProteinModelPortalP08763.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3390. M.EcoPI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PIRSFPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSPR00506. D21N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT3MO_BPP1
AccessionPrimary (citable) accession number: P08763
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries