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P08763

- T3MO_BPP1

UniProt

P08763 - T3MO_BPP1

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Protein
Type III restriction-modification system EcoPI enzyme mod
Gene
MOD
Organism
Enterobacteria phage P1 (Bacteriophage P1)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Binds the system-specific DNA recognition site 5'-AGACC-3'. Necessary for restriction and for methylation of A-3.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Protein family/group databases

REBASEi3390. M.EcoPI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type III restriction-modification system EcoPI enzyme mod (EC:2.1.1.72)
Short name:
M.EcoPI
Alternative name(s):
EcoPI methyltransferase
Gene namesi
Name:MOD
OrganismiEnterobacteria phage P1 (Bacteriophage P1)
Taxonomic identifieri10678 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePunalikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 646646Type III restriction-modification system EcoPI enzyme mod
PRO_0000088030Add
BLAST

Interactioni

Subunit structurei

Contains two different subunits: res and mod. Mod is a homotetramer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP08763.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1264Binding of S-adenosyl methionine Reviewed prediction

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PIRSFiPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08763-1 [UniParc]FASTAAdd to Basket

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MKKETIFSEV ETANSKQLAV LKANFPQCFD KNGAFIQEKL LEIIRASEVE    50
LSKESYSLNW LGKSYARLLA NLPPKTLLAE DKTHNQQEEN KNSQNLLIKG 100
DNLEVLKHMV NAYAEKVNMI YIDPPYNTGK DGFVYNDDRK FTPEQLSELA 150
GIELDEANRI LEFTTKGSSS HSAWLTFIYP RLYIARELLK EDGVIFISID 200
DNEDKQLGLL CDEVFGQGNF VAKLPTIMNL KGNHDNFGFS DTHEYIYVYA 250
KNKDVCSLGQ FDIDESEVEK EWDEDEYGLF KRADTLKRTG QDASRKSRPK 300
GWFPVFINSE NKVYVTDDDK PLNEDDYVLY PVSPTGEELS WSWGKKKIND 350
EFYNLIVIDI KDGKNIYKKQ RPALGELPTK KPKSIWYKPE YSTSTATTEL 400
KNLLGAKLFE GPKPVPLITD LVKIGTKKDS LVLDFFAGSG TTAEAVAYLN 450
EKDSGCRNFI CIQKDEVINK TKNAYSLGYR SIFEITKKRI QEVFKKSTTT 500
SDNAAKIGFK VIHTIDDFRA KVESELTLTN HTFFDDAVLT PEQYDALLTT 550
WCVYDGSLLT TPIEDVDLSG YTAHFCNGRL YLIAPNFTSE ALKALLQKLD 600
SDEDFAPNKV VFYGCNFESA KQRELNEALK SYANKKSIEL DLVVRN 646
Length:646
Mass (Da):73,486
Last modified:November 1, 1988 - v1
Checksum:iDB2D8724777A5412
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06287 Genomic DNA. Translation: CAA29614.1.
PIRiS01351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06287 Genomic DNA. Translation: CAA29614.1 .
PIRi S01351.

3D structure databases

ProteinModelPortali P08763.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3390. M.EcoPI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PIRSFi PIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSi PR00506. D21N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 3 hits.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants."
    Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A.
    J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5.

Entry informationi

Entry nameiT3MO_BPP1
AccessioniPrimary (citable) accession number: P08763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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