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Protein

Type III restriction-modification system EcoPI enzyme mod

Gene

MOD

Organism
Enterobacteria phage P1 (Bacteriophage P1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds the system-specific DNA recognition site 5'-AGACC-3'. Necessary for restriction and for methylation of A-3.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Protein family/group databases

REBASEi3390. M.EcoPI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type III restriction-modification system EcoPI enzyme mod (EC:2.1.1.72)
Short name:
M.EcoPI
Alternative name(s):
EcoPI methyltransferase
Gene namesi
Name:MOD
OrganismiEnterobacteria phage P1 (Bacteriophage P1)
Taxonomic identifieri10678 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePunalikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 646646Type III restriction-modification system EcoPI enzyme modPRO_0000088030Add
BLAST

Interactioni

Subunit structurei

Contains two different subunits: res and mod. Mod is a homotetramer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP08763.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1264Binding of S-adenosyl methionineSequence Analysis

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PIRSFiPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08763-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKETIFSEV ETANSKQLAV LKANFPQCFD KNGAFIQEKL LEIIRASEVE
60 70 80 90 100
LSKESYSLNW LGKSYARLLA NLPPKTLLAE DKTHNQQEEN KNSQNLLIKG
110 120 130 140 150
DNLEVLKHMV NAYAEKVNMI YIDPPYNTGK DGFVYNDDRK FTPEQLSELA
160 170 180 190 200
GIELDEANRI LEFTTKGSSS HSAWLTFIYP RLYIARELLK EDGVIFISID
210 220 230 240 250
DNEDKQLGLL CDEVFGQGNF VAKLPTIMNL KGNHDNFGFS DTHEYIYVYA
260 270 280 290 300
KNKDVCSLGQ FDIDESEVEK EWDEDEYGLF KRADTLKRTG QDASRKSRPK
310 320 330 340 350
GWFPVFINSE NKVYVTDDDK PLNEDDYVLY PVSPTGEELS WSWGKKKIND
360 370 380 390 400
EFYNLIVIDI KDGKNIYKKQ RPALGELPTK KPKSIWYKPE YSTSTATTEL
410 420 430 440 450
KNLLGAKLFE GPKPVPLITD LVKIGTKKDS LVLDFFAGSG TTAEAVAYLN
460 470 480 490 500
EKDSGCRNFI CIQKDEVINK TKNAYSLGYR SIFEITKKRI QEVFKKSTTT
510 520 530 540 550
SDNAAKIGFK VIHTIDDFRA KVESELTLTN HTFFDDAVLT PEQYDALLTT
560 570 580 590 600
WCVYDGSLLT TPIEDVDLSG YTAHFCNGRL YLIAPNFTSE ALKALLQKLD
610 620 630 640
SDEDFAPNKV VFYGCNFESA KQRELNEALK SYANKKSIEL DLVVRN
Length:646
Mass (Da):73,486
Last modified:November 1, 1988 - v1
Checksum:iDB2D8724777A5412
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06287 Genomic DNA. Translation: CAA29614.1.
PIRiS01351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06287 Genomic DNA. Translation: CAA29614.1.
PIRiS01351.

3D structure databases

ProteinModelPortaliP08763.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3390. M.EcoPI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PIRSFiPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants."
    Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A.
    J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5.

Entry informationi

Entry nameiT3MO_BPP1
AccessioniPrimary (citable) accession number: P08763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: January 7, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.