P08763 (T3MO_BPP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type III restriction-modification system EcoPI enzyme mod
Short name=M.EcoPI
EC=2.1.1.72

Alternative name(s):
EcoPI methyltransferase

Gene names

Name:

MOD

Organism

Enterobacteria phage P1 (Bacteriophage P1)

Taxonomic identifier

10678 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Punalikevirus

Virus host

Enterobacteriaceae [TaxID: 543]

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds the system-specific DNA recognition site 5'-AGACC-3'. Necessary for restriction and for methylation of A-3.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Contains two different subunits: res and mod. Mod is a homotetramer By similarity.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Type III restriction-modification system EcoPI enzyme mod		PRO_0000088030
Regions
	Region	123 – 126	4	Binding of S-adenosyl methionine Potential

Sequences

	Sequence		Length	Mass (Da)
	P08763 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: DB2D8724777A5412	FASTA	646	73,486
	10 20 30 40 50 60 MKKETIFSEV ETANSKQLAV LKANFPQCFD KNGAFIQEKL LEIIRASEVE LSKESYSLNW 70 80 90 100 110 120 LGKSYARLLA NLPPKTLLAE DKTHNQQEEN KNSQNLLIKG DNLEVLKHMV NAYAEKVNMI 130 140 150 160 170 180 YIDPPYNTGK DGFVYNDDRK FTPEQLSELA GIELDEANRI LEFTTKGSSS HSAWLTFIYP 190 200 210 220 230 240 RLYIARELLK EDGVIFISID DNEDKQLGLL CDEVFGQGNF VAKLPTIMNL KGNHDNFGFS 250 260 270 280 290 300 DTHEYIYVYA KNKDVCSLGQ FDIDESEVEK EWDEDEYGLF KRADTLKRTG QDASRKSRPK 310 320 330 340 350 360 GWFPVFINSE NKVYVTDDDK PLNEDDYVLY PVSPTGEELS WSWGKKKIND EFYNLIVIDI 370 380 390 400 410 420 KDGKNIYKKQ RPALGELPTK KPKSIWYKPE YSTSTATTEL KNLLGAKLFE GPKPVPLITD 430 440 450 460 470 480 LVKIGTKKDS LVLDFFAGSG TTAEAVAYLN EKDSGCRNFI CIQKDEVINK TKNAYSLGYR 490 500 510 520 530 540 SIFEITKKRI QEVFKKSTTT SDNAAKIGFK VIHTIDDFRA KVESELTLTN HTFFDDAVLT 550 560 570 580 590 600 PEQYDALLTT WCVYDGSLLT TPIEDVDLSG YTAHFCNGRL YLIAPNFTSE ALKALLQKLD 610 620 630 640 SDEDFAPNKV VFYGCNFESA KQRELNEALK SYANKKSIEL DLVVRN

References

[1]

"Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants." Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A. J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06287 Genomic DNA. Translation: CAA29614.1.
PIR	S01351.
3D structure databases
ProteinModelPortal	P08763.
ModBase	Search...
Protein family/group databases
REBASE	3390. M.EcoPI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PIRSF	PIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTS	PR00506. D21N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T3MO_BPP1

Accession

Primary (citable) accession number: P08763

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program