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Protein

Type III restriction-modification system EcoPI enzyme mod

Gene

MOD

Organism
Escherichia phage P1 (Bacteriophage P1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds the system-specific DNA recognition site 5'-AGACC-3'. Necessary for restriction and for methylation of A-3.

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3390 M.EcoPI

Names & Taxonomyi

Protein namesi
Recommended name:
Type III restriction-modification system EcoPI enzyme mod (EC:2.1.1.72)
Short name:
M.EcoPI
Alternative name(s):
EcoPI methyltransferase
Gene namesi
Name:MOD
OrganismiEscherichia phage P1 (Bacteriophage P1)
Taxonomic identifieri10678 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeP1virus
Virus hostiEnterobacteriaceae [TaxID: 543]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880301 – 646Type III restriction-modification system EcoPI enzyme modAdd BLAST646

Proteomic databases

PRIDEiP08763

Interactioni

Subunit structurei

Contains two different subunits: res and mod. Mod is a homotetramer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP08763
SMRiP08763
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 126Binding of S-adenosyl methionineSequence analysis4

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR002295 D21N6_MeTrfase
IPR002941 DNA_methylase_N4/N6
IPR002052 DNA_methylase_N6_adenine_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01555 N6_N4_Mtase, 1 hit
PIRSFiPIRSF015855 TypeIII_Mtase_mKpnI, 1 hit
PRINTSiPR00506 D21N6MTFRASE
SUPFAMiSSF53335 SSF53335, 3 hits
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P08763-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKETIFSEV ETANSKQLAV LKANFPQCFD KNGAFIQEKL LEIIRASEVE
60 70 80 90 100
LSKESYSLNW LGKSYARLLA NLPPKTLLAE DKTHNQQEEN KNSQNLLIKG
110 120 130 140 150
DNLEVLKHMV NAYAEKVNMI YIDPPYNTGK DGFVYNDDRK FTPEQLSELA
160 170 180 190 200
GIELDEANRI LEFTTKGSSS HSAWLTFIYP RLYIARELLK EDGVIFISID
210 220 230 240 250
DNEDKQLGLL CDEVFGQGNF VAKLPTIMNL KGNHDNFGFS DTHEYIYVYA
260 270 280 290 300
KNKDVCSLGQ FDIDESEVEK EWDEDEYGLF KRADTLKRTG QDASRKSRPK
310 320 330 340 350
GWFPVFINSE NKVYVTDDDK PLNEDDYVLY PVSPTGEELS WSWGKKKIND
360 370 380 390 400
EFYNLIVIDI KDGKNIYKKQ RPALGELPTK KPKSIWYKPE YSTSTATTEL
410 420 430 440 450
KNLLGAKLFE GPKPVPLITD LVKIGTKKDS LVLDFFAGSG TTAEAVAYLN
460 470 480 490 500
EKDSGCRNFI CIQKDEVINK TKNAYSLGYR SIFEITKKRI QEVFKKSTTT
510 520 530 540 550
SDNAAKIGFK VIHTIDDFRA KVESELTLTN HTFFDDAVLT PEQYDALLTT
560 570 580 590 600
WCVYDGSLLT TPIEDVDLSG YTAHFCNGRL YLIAPNFTSE ALKALLQKLD
610 620 630 640
SDEDFAPNKV VFYGCNFESA KQRELNEALK SYANKKSIEL DLVVRN
Length:646
Mass (Da):73,486
Last modified:November 1, 1988 - v1
Checksum:iDB2D8724777A5412
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06287 Genomic DNA Translation: CAA29614.1
PIRiS01351

Similar proteinsi

Entry informationi

Entry nameiT3MO_BPP1
AccessioniPrimary (citable) accession number: P08763
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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