Reviewed, UniProtKB/Swiss-Prot P08761 (MSRA_DROME)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase
    EC=1.8.4.11
Alternative name(s):
    Peptide-methionine (S)-S-oxide reductase
    Methionine-S-sulfoxide reductase
    Ecdysone-induced protein 28/29 kDa

Gene names

Name:	Eip71CD
Synonyms:	Eip28/29, MsrA
ORF Names:	CG7266

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. Ref.2
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin.
Induction	By ecdysone.
Sequence similarities	Belongs to the msrA Met sulfoxide reductase family.
Sequence caution	The sequence CAA28205.1 differs from that shown. Reason: Frameshift at position 222.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein metabolic process Inferred from electronic annotation. Source: InterPro salivary gland cell autophagic cell death Inferred from expression pattern. Source: FlyBase sulfur amino acid metabolic process Non-traceable author statement. Source: FlyBase
Molecular function	peptide-methionine-(S)-S-oxide reductase activity Ref.2 Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Eip28 (identifier: P08761-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Eip29 (identifier: P08761-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     79-82: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Peptide methionine sulfoxide reductase		PRO_0000138629
Natural variations
	Alternative sequence	79 – 82	4	Missing in isoform Eip29.		VSP_003279
Experimental info
	Sequence conflict	218 – 219	2	EA → V in AAO45213. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform Eip28 (A) [UniParc]. Last modified March 15, 2004. Version 2. Checksum: 8BB13B2092227D3E	FASTA	246	27,698
	10 20 30 40 50 60 MSLTITSSVT HPELKDLSTV RNEQKELNIS PVHDVNVTKA TATFGMGCFW GAESLYGATR 70 80 90 100 110 120 GVLRTTVGYA GGSSDLPTYR KMGDHTEVLE IDYDPTVISF KELLDLFWNN HEYGLTTPIK 130 140 150 160 170 180 RQYASLILYH DEEQKQVAHA SKLEEQERRA PEIITTEIAS KENFYPAEAY HQKYRLQGHK 190 200 210 220 230 240 DLASSLNLSP KLLQTSYVAT KLNGYLAGVG GIEQFKAEAE TMGLTPTQRQ YCYYHVEQNE GQGLYC
	Isoform Eip29 (B). Checksum: 29C4EE6B9742E50F	FASTA	242	27,119
	10 20 30 40 50 60 MSLTITSSVT HPELKDLSTV RNEQKELNIS PVHDVNVTKA TATFGMGCFW GAESLYGATR 70 80 90 100 110 120 GVLRTTVGYA GGSSDLPTGD HTEVLEIDYD PTVISFKELL DLFWNNHEYG LTTPIKRQYA 130 140 150 160 170 180 SLILYHDEEQ KQVAHASKLE EQERRAPEII TTEIASKENF YPAEAYHQKY RLQGHKDLAS 190 200 210 220 230 240 SLNLSPKLLQ TSYVATKLNG YLAGVGGIEQ FKAEAETMGL TPTQRQYCYY HVEQNEGQGL YC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the Eip28/29 gene, an ecdysone-inducible gene from Drosophila." Cherbas L., Schulz R.A., Koehler M.M.D., Savakis C., Cherbas P. J. Mol. Biol. 189:617-631(1986) [PubMed: 3097323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS EIP28 AND EIP29). Strain: Canton-S.
[2]	"Reaction mechanism, evolutionary analysis, and role of zinc in Drosophila methionine-R-sulfoxide reductase." Kumar R.A., Koc A., Cerny R.L., Gladyshev V.N. J. Biol. Chem. 277:37527-37535(2002) [PubMed: 12145281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM EIP28), FUNCTION.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM EIP29). Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X58286 Genomic DNA. Translation: CAA41223.1. Frameshift. X04024 Genomic DNA. Translation: CAA27657.1. Frameshift. X04024 Genomic DNA. Translation: CAA27658.1. Frameshift. X04521 mRNA. Translation: CAA28205.1. Frameshift. AF541958 mRNA. Translation: AAN28311.1. AE014296 Genomic DNA. Translation: AAF49630.2. AE014296 Genomic DNA. Translation: AAN11775.1. BT004857 mRNA. Translation: AAO45213.1.
PIR	A24254.
RefSeq	NP_524085.2. NP_730047.1.
UniGene	Dm.2545
3D structure databases
HSSP	HSSP built from PDB template 1FVA based on UniProtKB P54149.
ModBase	Search...
Protein-protein interaction databases
IntAct	P08761. 5 interactions.
Genome annotation databases
Ensembl	FBgn0000565. Drosophila melanogaster. [Contig view]
GeneID	39675.
KEGG	dme:Dmel_CG7266.
Organism-specific databases
FlyBase	FBgn0000565. Eip71CD.
Phylogenomic databases
OMA	P08761. HQKFELK.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-016766-MON.
BRENDA	1.8.4.11. 48.
Gene expression databases
ArrayExpress	P08761.
GermOnline	CG7266. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002569. MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit.
Pfam	PF01625. PMSR. 1 hit. [Graphical view]
ProDom	PD003489. PMSR. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00401. msrA. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	814812.

Entry information

Entry name

MSRA_DROME

Accession

Primary (citable) accession number: P08761
Secondary accession number(s): Q86NL3 , Q8IQM6, Q8IT52, Q9VUP3, Q9VUP4, Q9VUP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	March 15, 2004
Last modified:	June 16, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project