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Protein

GTP:AMP phosphotransferase AK3, mitochondrial

Gene

AK3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.UniRule annotation

Catalytic activityi

NTP + AMP = NDP + ADP.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38AMPUniRule annotation1 Publication1
Binding sitei43AMPUniRule annotation1
Binding sitei98AMPUniRule annotation1 Publication1
Binding sitei128GTPUniRule annotation1
Binding sitei161AMPUniRule annotation1
Binding sitei172AMPUniRule annotation1
Binding sitei201GTP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 22GTPUniRule annotation6
Nucleotide bindingi64 – 66AMPUniRule annotation1 Publication3
Nucleotide bindingi91 – 94AMPUniRule annotation4
Nucleotide bindingi137 – 138GTPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-983231 Factors involved in megakaryocyte development and platelet production
SABIO-RKiP08760

Names & Taxonomyi

Protein namesi
Recommended name:
GTP:AMP phosphotransferase AK3, mitochondrialUniRule annotation (EC:2.7.4.10UniRule annotation)
Alternative name(s):
Adenylate kinase 3UniRule annotation
Short name:
AK 3UniRule annotation
Adenylate kinase 3 alpha-like 1UniRule annotation
Gene namesi
Name:AK3UniRule annotation
Synonyms:AK3L1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Organism-specific databases

VGNCiVGNC:25772 AK3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation1 Publication
ChainiPRO_00001589212 – 227GTP:AMP phosphotransferase AK3, mitochondrialAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20N6-succinyllysineBy similarity1
Modified residuei29N6-acetyllysine; alternateBy similarity1
Modified residuei29N6-succinyllysine; alternateBy similarity1
Modified residuei34N6-acetyllysineBy similarity1
Modified residuei37PhosphoserineBy similarity1
Modified residuei57N6-succinyllysineBy similarity1
Modified residuei64N6-acetyllysine; alternateBy similarity1
Modified residuei64N6-succinyllysine; alternateBy similarity1
Modified residuei80N6-acetyllysine; alternateBy similarity1
Modified residuei80N6-succinyllysine; alternateBy similarity1
Modified residuei174N6-acetyllysine; alternateBy similarity1
Modified residuei174N6-succinyllysine; alternateBy similarity1
Modified residuei189N6-acetyllysine; alternateBy similarity1
Modified residuei189N6-succinyllysine; alternateBy similarity1
Modified residuei203N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP08760
PeptideAtlasiP08760
PRIDEiP08760

Expressioni

Gene expression databases

BgeeiENSBTAG00000017147

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

IntActiP08760, 1 interactor
STRINGi9913.ENSBTAP00000022789

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 13Combined sources5
Helixi20 – 30Combined sources11
Beta strandi34 – 37Combined sources4
Helixi38 – 47Combined sources10
Helixi51 – 61Combined sources11
Helixi68 – 82Combined sources15
Beta strandi87 – 91Combined sources5
Helixi96 – 103Combined sources8
Beta strandi110 – 115Combined sources6
Helixi118 – 125Combined sources8
Beta strandi128 – 130Combined sources3
Turni132 – 134Combined sources3
Beta strandi137 – 139Combined sources3
Turni140 – 142Combined sources3
Turni152 – 154Combined sources3
Helixi162 – 164Combined sources3
Helixi166 – 189Combined sources24
Beta strandi193 – 197Combined sources5
Helixi201 – 213Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AK3X-ray1.85A/B2-227[»]
ProteinModelPortaliP08760
SMRiP08760
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08760

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 66NMPbindUniRule annotation1 PublicationAdd BLAST30
Regioni127 – 164LIDUniRule annotation1 PublicationAdd BLAST38

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.UniRule annotation1 Publication

Sequence similaritiesi

Belongs to the adenylate kinase family. AK3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00550000074679
HOGENOMiHOG000238772
HOVERGENiHBG000458
InParanoidiP08760
KOiK00944
OMAiVKNRWIH
OrthoDBiEOG091G06BH
TreeFamiTF312916

Family and domain databases

CDDicd01428 ADK, 1 hit
HAMAPiMF_00235 Adenylate_kinase_Adk, 1 hit
MF_03169 Adenylate_kinase_AK3, 1 hit
InterProiView protein in InterPro
IPR006259 Adenyl_kin_sub
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR007862 Adenylate_kinase_lid-dom
IPR036193 ADK_active_lid_dom_sf
IPR028586 AK3/Ak4_mitochondrial
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PfamiView protein in Pfam
PF05191 ADK_lid, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 2 hits
SSF57774 SSF57774, 1 hit
TIGRFAMsiTIGR01351 adk, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGASARLLRA AIMGAPGSGK GTVSSRITKH FELKHLSSGD LLRDNMLRGT
60 70 80 90 100
EIGVLAKTFI DQGKLIPDDV MTRLVLHELK NLTQYNWLLD GFPRTLPQAE
110 120 130 140 150
ALDRAYQIDT VINLNVPFEV IKQRLTARWI HPGSGRVYNI EFNPPKTMGI
160 170 180 190 200
DDLTGEPLVQ REDDRPETVV KRLKAYEAQT EPVLEYYRKK GVLETFSGTE
210 220
TNKIWPHVYA FLQTKLPQRS QETSVTP
Length:227
Mass (Da):25,671
Last modified:January 23, 2007 - v3
Checksum:i2C29AEF195FBFBFA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11Missing (Ref. 2) Curated1
Sequence conflicti11Missing (PubMed:6088234).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25757 mRNA Translation: AAA30705.1
BC114157 mRNA Translation: AAI14158.1
D10376 Genomic DNA Translation: BAA01210.1
PIRiA34442
RefSeqiNP_776662.1, NM_174237.2
UniGeneiBt.5149

Genome annotation databases

EnsembliENSBTAT00000022789; ENSBTAP00000022789; ENSBTAG00000017147
GeneIDi281613
KEGGibta:281613

Similar proteinsi

Entry informationi

Entry nameiKAD3_BOVIN
AccessioniPrimary (citable) accession number: P08760
Secondary accession number(s): Q29RI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health