Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTP:AMP phosphotransferase AK3, mitochondrial

Gene

AK3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.UniRule annotation

Catalytic activityi

NTP + AMP = NDP + ADP.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38AMPUniRule annotation1 Publication1
Binding sitei43AMPUniRule annotation1
Binding sitei98AMPUniRule annotation1 Publication1
Binding sitei128GTPUniRule annotation1
Binding sitei161AMPUniRule annotation1
Binding sitei172AMPUniRule annotation1
Binding sitei201GTP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 22GTPUniRule annotation6
Nucleotide bindingi64 – 66AMPUniRule annotation1 Publication3
Nucleotide bindingi91 – 94AMPUniRule annotation4
Nucleotide bindingi137 – 138GTPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-983231. Factors involved in megakaryocyte development and platelet production.
SABIO-RKP08760.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP:AMP phosphotransferase AK3, mitochondrialUniRule annotation (EC:2.7.4.10UniRule annotation)
Alternative name(s):
Adenylate kinase 3UniRule annotation
Short name:
AK 3UniRule annotation
Adenylate kinase 3 alpha-like 1UniRule annotation
Gene namesi
Name:AK3UniRule annotation
Synonyms:AK3L1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

  • Mitochondrion matrix UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation1 Publication
ChainiPRO_00001589212 – 227GTP:AMP phosphotransferase AK3, mitochondrialAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20N6-succinyllysineBy similarity1
Modified residuei29N6-acetyllysine; alternateBy similarity1
Modified residuei29N6-succinyllysine; alternateBy similarity1
Modified residuei34N6-acetyllysineBy similarity1
Modified residuei37PhosphoserineBy similarity1
Modified residuei57N6-succinyllysineBy similarity1
Modified residuei64N6-acetyllysine; alternateBy similarity1
Modified residuei64N6-succinyllysine; alternateBy similarity1
Modified residuei80N6-acetyllysine; alternateBy similarity1
Modified residuei80N6-succinyllysine; alternateBy similarity1
Modified residuei174N6-acetyllysine; alternateBy similarity1
Modified residuei174N6-succinyllysine; alternateBy similarity1
Modified residuei189N6-acetyllysine; alternateBy similarity1
Modified residuei189N6-succinyllysine; alternateBy similarity1
Modified residuei203N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP08760.
PeptideAtlasiP08760.
PRIDEiP08760.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017147.

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

IntActiP08760. 1 interactor.
STRINGi9913.ENSBTAP00000022789.

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 13Combined sources5
Helixi20 – 30Combined sources11
Beta strandi34 – 37Combined sources4
Helixi38 – 47Combined sources10
Helixi51 – 61Combined sources11
Helixi68 – 82Combined sources15
Beta strandi87 – 91Combined sources5
Helixi96 – 103Combined sources8
Beta strandi110 – 115Combined sources6
Helixi118 – 125Combined sources8
Beta strandi128 – 130Combined sources3
Turni132 – 134Combined sources3
Beta strandi137 – 139Combined sources3
Turni140 – 142Combined sources3
Turni152 – 154Combined sources3
Helixi162 – 164Combined sources3
Helixi166 – 189Combined sources24
Beta strandi193 – 197Combined sources5
Helixi201 – 213Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AK3X-ray1.85A/B2-227[»]
ProteinModelPortaliP08760.
SMRiP08760.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08760.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 66NMPbindUniRule annotation1 PublicationAdd BLAST30
Regioni127 – 164LIDUniRule annotation1 PublicationAdd BLAST38

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.UniRule annotation1 Publication

Sequence similaritiesi

Belongs to the adenylate kinase family. AK3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00550000074679.
HOGENOMiHOG000238772.
HOVERGENiHBG000458.
InParanoidiP08760.
KOiK00944.
OMAiNVPFQTI.
OrthoDBiEOG091G06BH.
TreeFamiTF312916.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03169. Adenylate_kinase_AK3. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57774. SSF57774. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGASARLLRA AIMGAPGSGK GTVSSRITKH FELKHLSSGD LLRDNMLRGT
60 70 80 90 100
EIGVLAKTFI DQGKLIPDDV MTRLVLHELK NLTQYNWLLD GFPRTLPQAE
110 120 130 140 150
ALDRAYQIDT VINLNVPFEV IKQRLTARWI HPGSGRVYNI EFNPPKTMGI
160 170 180 190 200
DDLTGEPLVQ REDDRPETVV KRLKAYEAQT EPVLEYYRKK GVLETFSGTE
210 220
TNKIWPHVYA FLQTKLPQRS QETSVTP
Length:227
Mass (Da):25,671
Last modified:January 23, 2007 - v3
Checksum:i2C29AEF195FBFBFA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11Missing (Ref. 2) Curated1
Sequence conflicti11Missing (PubMed:6088234).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25757 mRNA. Translation: AAA30705.1.
BC114157 mRNA. Translation: AAI14158.1.
D10376 Genomic DNA. Translation: BAA01210.1.
PIRiA34442.
RefSeqiNP_776662.1. NM_174237.2.
UniGeneiBt.5149.

Genome annotation databases

EnsembliENSBTAT00000022789; ENSBTAP00000022789; ENSBTAG00000017147.
GeneIDi281613.
KEGGibta:281613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25757 mRNA. Translation: AAA30705.1.
BC114157 mRNA. Translation: AAI14158.1.
D10376 Genomic DNA. Translation: BAA01210.1.
PIRiA34442.
RefSeqiNP_776662.1. NM_174237.2.
UniGeneiBt.5149.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AK3X-ray1.85A/B2-227[»]
ProteinModelPortaliP08760.
SMRiP08760.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08760. 1 interactor.
STRINGi9913.ENSBTAP00000022789.

Proteomic databases

PaxDbiP08760.
PeptideAtlasiP08760.
PRIDEiP08760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000022789; ENSBTAP00000022789; ENSBTAG00000017147.
GeneIDi281613.
KEGGibta:281613.

Organism-specific databases

CTDi50808.

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00550000074679.
HOGENOMiHOG000238772.
HOVERGENiHBG000458.
InParanoidiP08760.
KOiK00944.
OMAiNVPFQTI.
OrthoDBiEOG091G06BH.
TreeFamiTF312916.

Enzyme and pathway databases

ReactomeiR-BTA-983231. Factors involved in megakaryocyte development and platelet production.
SABIO-RKP08760.

Miscellaneous databases

EvolutionaryTraceiP08760.

Gene expression databases

BgeeiENSBTAG00000017147.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03169. Adenylate_kinase_AK3. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57774. SSF57774. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAD3_BOVIN
AccessioniPrimary (citable) accession number: P08760
Secondary accession number(s): Q29RI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.