Reviewed, UniProtKB/Swiss-Prot P08753 (GNAI3_RAT)

Names and origin

Protein names

Recommended name:
    Guanine nucleotide-binding protein G(k) subunit alpha
Alternative name(s):
    G(i) alpha-3

Gene names

Name:	Gnai3
Synonyms:	Gnai-3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K+ channels.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1. Ref.4
Sequence similarities	Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Molecular function	Transducer
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro negative regulation of secretion Traceable author statement. Source: UniProtKB vesicle fusion Inferred from direct assay. Source: RGD
Cellular component	Golgi membrane Traceable author statement. Source: UniProtKB membrane fraction Inferred from direct assay. Source: RGD membrane raft Inferred from direct assay. Source: RGD zymogen granule Inferred from direct assay. Source: RGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW metabotropic serotonin receptor binding Inferred from physical interaction. Source: RGD signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RGS19	P49795	3	EBI-874897,EBI-874907	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 354	353	Guanine nucleotide-binding protein G(k) subunit alpha		PRO_0000203694
Regions
	Nucleotide binding	40 – 47	8	GTP By similarity
	Nucleotide binding	200 – 204	5	GTP By similarity
	Nucleotide binding	269 – 272	4	GTP By similarity
Amino acid modifications
	Modified residue	69	1	Phosphotyrosine By similarity
	Modified residue	354	1	Phosphotyrosine By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
	Lipidation	3	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08753-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A65D63CE5A32C777	FASTA	354	40,522
	10 20 30 40 50 60 MGCTLSAEDK AAVERSKMID RNLREDGEKA AKEVKLLLLG AGESGKSTIV KQMKIIHEDG 70 80 90 100 110 120 YSEDECKQYK VVVYSNTIQS IIAIIRAMGR LKIDFGEAAR ADDARQLFVL AGSAEEGVMT 130 140 150 160 170 180 SELAGVIKRL WRDGGVQACF SRSREYQLND SASYYLNDLD RISQTNYIPT QQDVLRTRVK 190 200 210 220 230 240 TTGIVETHFT FKELYFKMFD VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM 250 260 270 280 290 300 NRMHESMKLF DSICNNKWFT DTSIILFLNK KDLFEEKIKR SPLTICYPEY TGSNTYEEAA 310 320 330 340 350 AYIQCQFEDL NRRKDTKEVY THFTCATDTK NVQFVFDAVT DVIIKNNLKE CGLY

References

[1]	"Presence of three distinct molecular species of Gi protein alpha subunit. Structure of rat cDNAs and human genomic DNAs." Itoh H., Toyama R., Kozasa T., Tsukamoto T., Matsuoka M., Kaziro Y. J. Biol. Chem. 263:6656-6664(1988) [PubMed: 2834384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium." Jones D.T., Reed R.R. J. Biol. Chem. 262:14241-14249(1987) [PubMed: 2820999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Purification and characterization of Go alpha and three types of Gi alpha after expression in Escherichia coli." Linder M.E., Ewald D.A., Miller R.J., Gilman A.G. J. Biol. Chem. 265:8243-8251(1990) [PubMed: 2159473] [Abstract] Cited for: PROTEIN SEQUENCE OF 111-125.
[4]	"Activator of G protein signaling 3 is a guanine dissociation inhibitor for Galpha i subunits." de Vries L., Fischer T., Tronchere H., Brothers G.M., Strockbine B., Siderovski D.P., Farquhar M.G. Proc. Natl. Acad. Sci. U.S.A. 97:14364-14369(2000) [PubMed: 11121039] [Abstract] Cited for: INTERACTION WITH GPSM1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03219 mRNA. Translation: AAA41224.1. M20713 mRNA. Translation: AAA40823.1.
IPI	IPI00231726.
PIR	RGRTI3. E27423.
RefSeq	NP_037238.1.
UniGene	Rn.4368
3D structure databases
SMR	P08753. Positions 5-348.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:608N.
IntAct	P08753. 1 interaction.
STRING	P08753.
Proteomic databases
PRIDE	P08753.
Genome annotation databases
Ensembl	ENSRNOT00000026710; ENSRNOP00000026710; ENSRNOG00000019465; Rattus norvegicus. [Genome view]
GeneID	25643.
KEGG	rno:25643.
UCSC	NM_013106. rat.
Organism-specific databases
CTD	25643.
RGD	2714. Gnai3.
Phylogenomic databases
HOVERGEN	P08753.
OMA	RISQTNY
OrthoDB	EOG9P5NVF
Gene expression databases
ArrayExpress	P08753.
Genevestigator	P08753.
GermOnline	ENSRNOG00000019465. Rattus norvegicus.
Family and domain databases
InterPro	IPR001408. Gprotein_alpha_I. IPR001019. Gprotein_alpha_su. IPR011025. GproteinA_insert. [Graphical view]
PANTHER	PTHR10218. Gprotein_alph_bd. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00441. GPROTEINAI.
SMART	SM00275. G_alpha. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	607491.

Entry information

Entry name

GNAI3_RAT

Accession

Primary (citable) accession number: P08753

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)