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P08752

- GNAI2_MOUSE

UniProt

P08752 - GNAI2_MOUSE

Protein

Guanine nucleotide-binding protein G(i) subunit alpha-2

Gene

Gnai2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 5 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471MagnesiumBy similarity
    Metal bindingi182 – 1821MagnesiumBy similarity
    Binding sitei327 – 3271GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi40 – 478GTPBy similarity
    Nucleotide bindingi176 – 1827GTPBy similarity
    Nucleotide bindingi201 – 2055GTPBy similarity
    Nucleotide bindingi270 – 2734GTPBy similarity

    GO - Molecular functioni

    1. G-protein beta/gamma-subunit complex binding Source: RefGenome
    2. G-protein coupled receptor binding Source: RefGenome
    3. GTPase activity Source: RefGenome
    4. GTP binding Source: MGI
    5. metal ion binding Source: UniProtKB-KW
    6. protein binding Source: MGI
    7. signal transducer activity Source: RefGenome

    GO - Biological processi

    1. activation of MAPKK activity Source: Ensembl
    2. adenosine receptor signaling pathway Source: RefGenome
    3. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: MGI
    4. cell cycle Source: UniProtKB-KW
    5. cell division Source: UniProtKB
    6. cell proliferation Source: MGI
    7. gamma-aminobutyric acid signaling pathway Source: RefGenome
    8. G-protein coupled acetylcholine receptor signaling pathway Source: MGI
    9. negative regulation of synaptic transmission Source: Ensembl
    10. positive regulation of cell proliferation Source: Ensembl
    11. regulation of calcium ion transport Source: Ensembl

    Keywords - Molecular functioni

    Transducer

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_205726. G-protein activation.
    REACT_222824. Adenylate cyclase inhibitory pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(i) subunit alpha-2
    Alternative name(s):
    Adenylate cyclase-inhibiting G alpha protein
    Gene namesi
    Name:Gnai2
    Synonyms:Gnai-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:95772. Gnai2.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cell membrane By similarity
    Note: Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody By similarity.By similarity

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. cytosol Source: Ensembl
    4. heterotrimeric G-protein complex Source: RefGenome
    5. membrane raft Source: Ensembl
    6. midbody Source: UniProtKB
    7. nucleus Source: Ensembl
    8. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 355354Guanine nucleotide-binding protein G(i) subunit alpha-2PRO_0000203681Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi3 – 31S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate

    Proteomic databases

    MaxQBiP08752.
    PaxDbiP08752.
    PRIDEiP08752.

    PTM databases

    PhosphoSiteiP08752.

    Expressioni

    Gene expression databases

    ArrayExpressiP08752.
    BgeeiP08752.
    GenevestigatoriP08752.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UNC5B. Interacts with GPSM1. Interacts with RGS12 and RGS14 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi199967. 2 interactions.
    DIPiDIP-605N.
    IntActiP08752. 6 interactions.
    MINTiMINT-4096273.
    STRINGi10090.ENSMUSP00000057543.

    Structurei

    3D structure databases

    ProteinModelPortaliP08752.
    SMRiP08752. Positions 5-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG322962.
    GeneTreeiENSGT00690000101958.
    HOGENOMiHOG000038730.
    HOVERGENiHBG063184.
    InParanoidiQ3TXK7.
    KOiK04630.
    OMAiEYAGANK.
    OrthoDBiEOG72C50B.
    TreeFamiTF300673.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08752-1 [UniParc]FASTAAdd to Basket

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    MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV    50
    KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPQR 100
    ADDARQLFAL SCAAEEQGML PEDLSGVIRR LWADHGVQAC FGRSREYQLN 150
    DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF 200
    DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL 250
    FDSICNNKWF TDTSIILFLN KKDLFEEKIT QSSLTICFPE YTGANKYDEA 300
    ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK 350
    DCGLF 355
    Length:355
    Mass (Da):40,489
    Last modified:July 27, 2011 - v5
    Checksum:i90AC64AFA713493E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti82 – 821M → I in AAB30632. (PubMed:8170357)Curated
    Sequence conflicti82 – 821M → L in AAA37692. (PubMed:3092218)Curated
    Sequence conflicti87 – 871A → R in AAA37692. (PubMed:3092218)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13963 mRNA. Translation: AAA37692.1.
    AK157998 mRNA. Translation: BAE34308.1.
    AK159222 mRNA. Translation: BAE34909.1.
    AK167388 mRNA. Translation: BAE39478.1.
    BC065159 mRNA. Translation: AAH65159.1.
    S71213 mRNA. Translation: AAB30632.2.
    CCDSiCCDS23502.1.
    PIRiB25889. RGMSI2.
    RefSeqiNP_032164.2. NM_008138.4.
    XP_006511700.1. XM_006511637.1.
    UniGeneiMm.196464.

    Genome annotation databases

    EnsembliENSMUST00000055704; ENSMUSP00000057543; ENSMUSG00000032562.
    GeneIDi14678.
    KEGGimmu:14678.
    UCSCiuc009rml.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13963 mRNA. Translation: AAA37692.1 .
    AK157998 mRNA. Translation: BAE34308.1 .
    AK159222 mRNA. Translation: BAE34909.1 .
    AK167388 mRNA. Translation: BAE39478.1 .
    BC065159 mRNA. Translation: AAH65159.1 .
    S71213 mRNA. Translation: AAB30632.2 .
    CCDSi CCDS23502.1.
    PIRi B25889. RGMSI2.
    RefSeqi NP_032164.2. NM_008138.4.
    XP_006511700.1. XM_006511637.1.
    UniGenei Mm.196464.

    3D structure databases

    ProteinModelPortali P08752.
    SMRi P08752. Positions 5-354.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199967. 2 interactions.
    DIPi DIP-605N.
    IntActi P08752. 6 interactions.
    MINTi MINT-4096273.
    STRINGi 10090.ENSMUSP00000057543.

    PTM databases

    PhosphoSitei P08752.

    Proteomic databases

    MaxQBi P08752.
    PaxDbi P08752.
    PRIDEi P08752.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000055704 ; ENSMUSP00000057543 ; ENSMUSG00000032562 .
    GeneIDi 14678.
    KEGGi mmu:14678.
    UCSCi uc009rml.1. mouse.

    Organism-specific databases

    CTDi 2771.
    MGIi MGI:95772. Gnai2.

    Phylogenomic databases

    eggNOGi NOG322962.
    GeneTreei ENSGT00690000101958.
    HOGENOMi HOG000038730.
    HOVERGENi HBG063184.
    InParanoidi Q3TXK7.
    KOi K04630.
    OMAi EYAGANK.
    OrthoDBi EOG72C50B.
    TreeFami TF300673.

    Enzyme and pathway databases

    Reactomei REACT_205726. G-protein activation.
    REACT_222824. Adenylate cyclase inhibitory pathway.

    Miscellaneous databases

    ChiTaRSi GNAI2. mouse.
    NextBioi 286578.
    PROi P08752.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P08752.
    Bgeei P08752.
    Genevestigatori P08752.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Inhibitory and stimulatory G proteins of adenylate cyclase: cDNA and amino acid sequences of the alpha chains."
      Sullivan K.A., Liao Y.-C., Alborzi A., Beiderman B., Chang F.-H., Masters S.B., Levinson A.D., Bourne H.R.
      Proc. Natl. Acad. Sci. U.S.A. 83:6687-6691(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Inner ear and Placenta.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "G protein Gi2 alpha in the cochlea: cloning and selective occurrence in receptor cells."
      Tachibana M., Asano T., Wilcox E., Yokotani N., Rivolta M.N., Fex J.
      Brain Res. Mol. Brain Res. 21:355-358(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-355.
    5. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 36-46; 55-67; 146-162; 182-193; 199-206; 250-258; 279-296 AND 319-331, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiGNAI2_MOUSE
    AccessioniPrimary (citable) accession number: P08752
    Secondary accession number(s): Q3TXK7, Q6P1C0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 143 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3