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Protein

Guanine nucleotide-binding protein G(i) subunit alpha-2

Gene

Gnai2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471MagnesiumBy similarity
Metal bindingi182 – 1821MagnesiumBy similarity
Binding sitei327 – 3271GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 478GTPBy similarity
Nucleotide bindingi176 – 1827GTPBy similarity
Nucleotide bindingi201 – 2055GTPBy similarity
Nucleotide bindingi270 – 2734GTPBy similarity

GO - Molecular functioni

  1. G-protein beta/gamma-subunit complex binding Source: GO_Central
  2. G-protein coupled receptor binding Source: GO_Central
  3. GTPase activity Source: GO_Central
  4. GTP binding Source: MGI
  5. metal ion binding Source: UniProtKB-KW
  6. signal transducer activity Source: GO_Central

GO - Biological processi

  1. activation of MAPKK activity Source: Ensembl
  2. adenosine receptor signaling pathway Source: GO_Central
  3. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: MGI
  4. cell cycle Source: UniProtKB-KW
  5. cell division Source: UniProtKB
  6. cell proliferation Source: MGI
  7. gamma-aminobutyric acid signaling pathway Source: GO_Central
  8. G-protein coupled acetylcholine receptor signaling pathway Source: MGI
  9. intracellular signal transduction Source: Ensembl
  10. metabolic process Source: GOC
  11. negative regulation of synaptic transmission Source: Ensembl
  12. positive regulation of cell proliferation Source: Ensembl
  13. regulation of calcium ion transport Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_272374. Adrenaline,noradrenaline inhibits insulin secretion.
REACT_296380. G alpha (z) signalling events.
REACT_303206. G-protein activation.
REACT_309686. Adenylate cyclase inhibitory pathway.
REACT_313192. G alpha (s) signalling events.
REACT_319215. PLC beta mediated events.
REACT_330925. ADP signalling through P2Y purinoceptor 12.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(i) subunit alpha-2
Alternative name(s):
Adenylate cyclase-inhibiting G alpha protein
Gene namesi
Name:Gnai2
Synonyms:Gnai-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:95772. Gnai2.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cell membrane By similarity
Note: Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody (By similarity).By similarity

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. cytosol Source: Ensembl
  4. extracellular vesicular exosome Source: MGI
  5. heterotrimeric G-protein complex Source: GO_Central
  6. membrane Source: MGI
  7. membrane raft Source: Ensembl
  8. midbody Source: UniProtKB
  9. nucleoplasm Source: MGI
  10. plasma membrane Source: UniProtKB
  11. vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 355354Guanine nucleotide-binding protein G(i) subunit alpha-2PRO_0000203681Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

MaxQBiP08752.
PaxDbiP08752.
PRIDEiP08752.

PTM databases

PhosphoSiteiP08752.

Expressioni

Gene expression databases

BgeeiP08752.
ExpressionAtlasiP08752. baseline and differential.
GenevestigatoriP08752.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UNC5B. Interacts with GPSM1. Interacts with RGS12 and RGS14 (By similarity).By similarity

Protein-protein interaction databases

BioGridi199967. 2 interactions.
DIPiDIP-605N.
IntActiP08752. 6 interactions.
MINTiMINT-4096273.
STRINGi10090.ENSMUSP00000057543.

Structurei

3D structure databases

ProteinModelPortaliP08752.
SMRiP08752. Positions 5-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiNOG322962.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP08752.
KOiK04630.
OMAiWADQGVQ.
OrthoDBiEOG72C50B.
TreeFamiTF300673.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPQR
110 120 130 140 150
ADDARQLFAL SCAAEEQGML PEDLSGVIRR LWADHGVQAC FGRSREYQLN
160 170 180 190 200
DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF
210 220 230 240 250
DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL
260 270 280 290 300
FDSICNNKWF TDTSIILFLN KKDLFEEKIT QSSLTICFPE YTGANKYDEA
310 320 330 340 350
ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK

DCGLF
Length:355
Mass (Da):40,489
Last modified:July 27, 2011 - v5
Checksum:i90AC64AFA713493E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821M → I in AAB30632 (PubMed:8170357).Curated
Sequence conflicti82 – 821M → L in AAA37692 (PubMed:3092218).Curated
Sequence conflicti87 – 871A → R in AAA37692 (PubMed:3092218).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13963 mRNA. Translation: AAA37692.1.
AK157998 mRNA. Translation: BAE34308.1.
AK159222 mRNA. Translation: BAE34909.1.
AK167388 mRNA. Translation: BAE39478.1.
BC065159 mRNA. Translation: AAH65159.1.
S71213 mRNA. Translation: AAB30632.2.
CCDSiCCDS23502.1.
PIRiB25889. RGMSI2.
RefSeqiNP_032164.2. NM_008138.4.
XP_006511700.1. XM_006511637.2.
UniGeneiMm.196464.

Genome annotation databases

EnsembliENSMUST00000055704; ENSMUSP00000057543; ENSMUSG00000032562.
ENSMUST00000192615; ENSMUSP00000142326; ENSMUSG00000032562.
GeneIDi14678.
KEGGimmu:14678.
UCSCiuc009rml.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13963 mRNA. Translation: AAA37692.1.
AK157998 mRNA. Translation: BAE34308.1.
AK159222 mRNA. Translation: BAE34909.1.
AK167388 mRNA. Translation: BAE39478.1.
BC065159 mRNA. Translation: AAH65159.1.
S71213 mRNA. Translation: AAB30632.2.
CCDSiCCDS23502.1.
PIRiB25889. RGMSI2.
RefSeqiNP_032164.2. NM_008138.4.
XP_006511700.1. XM_006511637.2.
UniGeneiMm.196464.

3D structure databases

ProteinModelPortaliP08752.
SMRiP08752. Positions 5-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199967. 2 interactions.
DIPiDIP-605N.
IntActiP08752. 6 interactions.
MINTiMINT-4096273.
STRINGi10090.ENSMUSP00000057543.

PTM databases

PhosphoSiteiP08752.

Proteomic databases

MaxQBiP08752.
PaxDbiP08752.
PRIDEiP08752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055704; ENSMUSP00000057543; ENSMUSG00000032562.
ENSMUST00000192615; ENSMUSP00000142326; ENSMUSG00000032562.
GeneIDi14678.
KEGGimmu:14678.
UCSCiuc009rml.1. mouse.

Organism-specific databases

CTDi2771.
MGIiMGI:95772. Gnai2.

Phylogenomic databases

eggNOGiNOG322962.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP08752.
KOiK04630.
OMAiWADQGVQ.
OrthoDBiEOG72C50B.
TreeFamiTF300673.

Enzyme and pathway databases

ReactomeiREACT_272374. Adrenaline,noradrenaline inhibits insulin secretion.
REACT_296380. G alpha (z) signalling events.
REACT_303206. G-protein activation.
REACT_309686. Adenylate cyclase inhibitory pathway.
REACT_313192. G alpha (s) signalling events.
REACT_319215. PLC beta mediated events.
REACT_330925. ADP signalling through P2Y purinoceptor 12.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

ChiTaRSiGnai2. mouse.
NextBioi286578.
PROiP08752.
SOURCEiSearch...

Gene expression databases

BgeeiP08752.
ExpressionAtlasiP08752. baseline and differential.
GenevestigatoriP08752.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Inhibitory and stimulatory G proteins of adenylate cyclase: cDNA and amino acid sequences of the alpha chains."
    Sullivan K.A., Liao Y.-C., Alborzi A., Beiderman B., Chang F.-H., Masters S.B., Levinson A.D., Bourne H.R.
    Proc. Natl. Acad. Sci. U.S.A. 83:6687-6691(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Inner ear and Placenta.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "G protein Gi2 alpha in the cochlea: cloning and selective occurrence in receptor cells."
    Tachibana M., Asano T., Wilcox E., Yokotani N., Rivolta M.N., Fex J.
    Brain Res. Mol. Brain Res. 21:355-358(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-355.
  5. Lubec G., Kang S.U.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 36-46; 55-67; 146-162; 182-193; 199-206; 250-258; 279-296 AND 319-331, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiGNAI2_MOUSE
AccessioniPrimary (citable) accession number: P08752
Secondary accession number(s): Q3TXK7, Q6P1C0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 149 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.