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Protein

D-alanyl-D-alanine carboxypeptidase DacA

Gene

dacA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.

Catalytic activityi

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Acyl-ester intermediate1 Publication
Active sitei70 – 701Proton acceptorBy similarity
Active sitei131 – 1311By similarity
Binding sitei258 – 2581SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciBSUB:BSU00100-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

MEROPSiS11.001.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanyl-D-alanine carboxypeptidase DacA (EC:3.4.16.4)
Short name:
CPase
Short name:
DD-carboxypeptidase
Short name:
DD-peptidase
Alternative name(s):
Penicillin-binding protein 5
Short name:
PBP-5
Gene namesi
Name:dacA
Ordered Locus Names:BSU00100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3112381.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 31311 PublicationAdd
BLAST
Chaini32 – 443412D-alanyl-D-alanine carboxypeptidase DacAPRO_0000027228Add
BLAST

Proteomic databases

PaxDbiP08750.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100000050.

Structurei

3D structure databases

ProteinModelPortaliP08750.
SMRiP08750. Positions 36-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S11 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1686. LUCA.
HOGENOMiHOG000086625.
InParanoidiP08750.
KOiK07258.
OMAiYVYEISQ.
PhylomeDBiP08750.

Family and domain databases

Gene3Di2.60.410.10. 1 hit.
3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015956. Peniciliin-bd_prot-assoc.
IPR018044. Peptidase_S11.
IPR012907. Peptidase_S11_C.
IPR001967. Peptidase_S11_N.
[Graphical view]
PfamiPF07943. PBP5_C. 1 hit.
PF00768. Peptidase_S11. 1 hit.
[Graphical view]
PRINTSiPR00725. DADACBPTASE1.
SMARTiSM00936. PBP5_C. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
SSF69189. SSF69189. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKKCKQLL MSLVVLTLAV TCLAPMSKAK AASDPIDINA SAAIMIEASS
60 70 80 90 100
GKILYSKNAD KRLPIASMTK MMTEYLLLEA IDQGKVKWDQ TYTPDDYVYE
110 120 130 140 150
ISQDNSLSNV PLRKDGKYTV KELYQATAIY SANAAAIAIA EIVAGSETKF
160 170 180 190 200
VEKMNAKAKE LGLTDYKFVN ATGLENKDLH GHQPEGTSVN EESEVSAKDM
210 220 230 240 250
AVLADHLITD YPEILETSSI AKTKFREGTD DEMDMPNWNF MLKGLVSEYK
260 270 280 290 300
KATVDGLKTG STDSAGSCFT GTAERNGMRV ITVVLNAKGN LHTGRFDETK
310 320 330 340 350
KMFDYAFDNF SMKEIYAEGD QVKGHKTISV DKGKEKEVGI VTNKAFSLPV
360 370 380 390 400
KNGEEKNYKA KVTLNKDNLT APVKKGTKVG KLTAEYTGDE KDYGFLNSDL
410 420 430 440
AGVDLVTKEN VEKANWFVLT MRSIGGFFAG IWGSIVDTVT GWF
Length:443
Mass (Da):48,636
Last modified:October 1, 1994 - v3
Checksum:iDA6C5B0307D7C117
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001E → Q AA sequence (PubMed:6768745).Curated
Sequence conflicti227 – 2271E → Q in AAA22375 (PubMed:3087956).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05246.1.
AL009126 Genomic DNA. Translation: CAB11786.1.
M13766 Genomic DNA. Translation: AAA22375.1.
PIRiS66040.
RefSeqiNP_387891.1. NC_000964.3.
WP_009966224.1. NZ_JNCM01000024.1.

Genome annotation databases

EnsemblBacteriaiCAB11786; CAB11786; BSU00100.
GeneIDi940000.
KEGGibsu:BSU00100.
PATRICi18971479. VBIBacSub10457_0011.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26185 Genomic DNA. Translation: BAA05246.1.
AL009126 Genomic DNA. Translation: CAB11786.1.
M13766 Genomic DNA. Translation: AAA22375.1.
PIRiS66040.
RefSeqiNP_387891.1. NC_000964.3.
WP_009966224.1. NZ_JNCM01000024.1.

3D structure databases

ProteinModelPortaliP08750.
SMRiP08750. Positions 36-412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100000050.

Chemistry

ChEMBLiCHEMBL3112381.

Protein family/group databases

MEROPSiS11.001.

Proteomic databases

PaxDbiP08750.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11786; CAB11786; BSU00100.
GeneIDi940000.
KEGGibsu:BSU00100.
PATRICi18971479. VBIBacSub10457_0011.

Phylogenomic databases

eggNOGiCOG1686. LUCA.
HOGENOMiHOG000086625.
InParanoidiP08750.
KOiK07258.
OMAiYVYEISQ.
PhylomeDBiP08750.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciBSUB:BSU00100-MONOMER.

Miscellaneous databases

PROiP08750.

Family and domain databases

Gene3Di2.60.410.10. 1 hit.
3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015956. Peniciliin-bd_prot-assoc.
IPR018044. Peptidase_S11.
IPR012907. Peptidase_S11_C.
IPR001967. Peptidase_S11_N.
[Graphical view]
PfamiPF07943. PBP5_C. 1 hit.
PF00768. Peptidase_S11. 1 hit.
[Graphical view]
PRINTSiPR00725. DADACBPTASE1.
SMARTiSM00936. PBP5_C. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
SSF69189. SSF69189. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDACA_BACSU
AccessioniPrimary (citable) accession number: P08750
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1994
Last modified: September 7, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.