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Protein

Elongation factor 1-alpha 1

Gene

Ef1alpha48D

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTPBy similarity
Nucleotide bindingi91 – 955GTPBy similarity
Nucleotide bindingi153 – 1564GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: FlyBase

GO - Biological processi

  • determination of adult lifespan Source: FlyBase
  • translation Source: FlyBase
  • translational elongation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-3371511. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alpha 1
Short name:
EF-1-alpha-1
Alternative name(s):
50 kDa female-specific protein
Gene namesi
Name:Ef1alpha48D
Synonyms:EF1B, F1
ORF Names:CG8280
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0000556. Ef1alpha48D.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • cytosol Source: FlyBase
  • eukaryotic translation elongation factor 1 complex Source: FlyBase
  • lipid particle Source: FlyBase
  • microtubule associated complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Elongation factor 1-alpha 1PRO_0000090906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei301 – 30115-glutamyl glycerylphosphorylethanolamine1 Publication
Modified residuei374 – 37415-glutamyl glycerylphosphorylethanolamineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08736.
PRIDEiP08736.

Expressioni

Gene expression databases

BgeeiFBgn0000556.
ExpressionAtlasiP08736. differential.
GenevisibleiP08736. DM.

Interactioni

Protein-protein interaction databases

BioGridi62056. 56 interactions.
IntActiP08736. 5 interactions.
MINTiMINT-284766.
STRINGi7227.FBpp0304260.

Structurei

3D structure databases

ProteinModelPortaliP08736.
SMRiP08736. Positions 4-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 242238tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 218G1By similarity
Regioni70 – 745G2By similarity
Regioni91 – 944G3By similarity
Regioni153 – 1564G4By similarity
Regioni194 – 1963G5By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0052. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00670000097815.
InParanoidiP08736.
KOiK03231.
OMAiNMNWFKG.
OrthoDBiEOG091G05LW.
PhylomeDBiP08736.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKEKIHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAQEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDIALWKF ETAKYYVTII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIVAAGT GEFEAGISKN GQTREHALLA FTLGVKQLIV
160 170 180 190 200
GVNKMDSSEP PYSEARYEEI KKEVSSYIKK IGYNPAAVAF VPISGWHGDN
210 220 230 240 250
MLEPSTNMPW FKGWKVERKE GNADGKTLID ALDAILPPAR PTDKALRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG VLKPGTVVVF APANITTEVK SVEMHHEALQ
310 320 330 340 350
EAVPGDNVGF NVKNVSVKEL RRGYVAGDSK ANPPKGAADF TAQVIVLNHP
360 370 380 390 400
GQIANGYTPV LDCHTAHIAC KFAEIKEKVD RRSGKTTEEN PKFIKSGDAA
410 420 430 440 450
IVNLVPSKPL CVEAFQEFPP LGRFAVRDMR QTVAVGVIKA VNFKDASGGK
460
VTKAAEKATK GKK
Length:463
Mass (Da):50,307
Last modified:June 21, 2005 - v2
Checksum:i33ECDCDB4DAE8BBA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1163LIV → QID in AAA28526 (PubMed:16593598).Curated
Sequence conflicti114 – 1163LIV → QID in CAA29993 (PubMed:3131735).Curated
Sequence conflicti131 – 1311G → D in AAA28526 (PubMed:16593598).Curated
Sequence conflicti131 – 1311G → D in CAA29993 (PubMed:3131735).Curated
Sequence conflicti181 – 1811I → V in AAA28526 (PubMed:16593598).Curated
Sequence conflicti181 – 1811I → V in CAA29993 (PubMed:3131735).Curated
Sequence conflicti215 – 2173KVE → EVG in AAA28526 (PubMed:16593598).Curated
Sequence conflicti215 – 2173KVE → EVG in CAA29993 (PubMed:3131735).Curated
Sequence conflicti229 – 2291I → V in AAA28526 (PubMed:16593598).Curated
Sequence conflicti229 – 2291I → V in CAA29993 (PubMed:3131735).Curated
Sequence conflicti376 – 3761K → L in AAA28526 (PubMed:16593598).Curated
Sequence conflicti376 – 3761K → L in CAA29993 (PubMed:3131735).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11744 Genomic DNA. Translation: AAA28526.1.
X06869 Genomic DNA. Translation: CAA29993.1.
AE013599 Genomic DNA. Translation: AAF58608.1.
BT011039 mRNA. Translation: AAR30199.1.
PIRiS00676.
RefSeqiNP_001286321.1. NM_001299392.1.
NP_001286322.1. NM_001299393.1.
NP_477375.1. NM_058027.5.
NP_725085.1. NM_165850.3.
UniGeneiDm.29457.

Genome annotation databases

EnsemblMetazoaiFBtr0088035; FBpp0087142; FBgn0000556.
FBtr0331927; FBpp0304260; FBgn0000556.
FBtr0345022; FBpp0311272; FBgn0000556.
FBtr0345023; FBpp0311273; FBgn0000556.
GeneIDi36271.
KEGGidme:Dmel_CG8280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11744 Genomic DNA. Translation: AAA28526.1.
X06869 Genomic DNA. Translation: CAA29993.1.
AE013599 Genomic DNA. Translation: AAF58608.1.
BT011039 mRNA. Translation: AAR30199.1.
PIRiS00676.
RefSeqiNP_001286321.1. NM_001299392.1.
NP_001286322.1. NM_001299393.1.
NP_477375.1. NM_058027.5.
NP_725085.1. NM_165850.3.
UniGeneiDm.29457.

3D structure databases

ProteinModelPortaliP08736.
SMRiP08736. Positions 4-444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62056. 56 interactions.
IntActiP08736. 5 interactions.
MINTiMINT-284766.
STRINGi7227.FBpp0304260.

Proteomic databases

PaxDbiP08736.
PRIDEiP08736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088035; FBpp0087142; FBgn0000556.
FBtr0331927; FBpp0304260; FBgn0000556.
FBtr0345022; FBpp0311272; FBgn0000556.
FBtr0345023; FBpp0311273; FBgn0000556.
GeneIDi36271.
KEGGidme:Dmel_CG8280.

Organism-specific databases

CTDi36271.
FlyBaseiFBgn0000556. Ef1alpha48D.

Phylogenomic databases

eggNOGiKOG0052. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00670000097815.
InParanoidiP08736.
KOiK03231.
OMAiNMNWFKG.
OrthoDBiEOG091G05LW.
PhylomeDBiP08736.

Enzyme and pathway databases

ReactomeiR-DME-3371511. HSF1 activation.

Miscellaneous databases

ChiTaRSiEf1alpha48D. fly.
GenomeRNAii36271.
PROiP08736.

Gene expression databases

BgeeiFBgn0000556.
ExpressionAtlasiP08736. differential.
GenevisibleiP08736. DM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1A1_DROME
AccessioniPrimary (citable) accession number: P08736
Secondary accession number(s): Q0E9B9, Q9V631
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 21, 2005
Last modified: September 7, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.