ID   G3PC_MAIZE              Reviewed;         337 AA.
AC   P08735;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 2.
DT   16-JUN-2009, entry version 74.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase, cytosolic 1;
DE            EC=1.2.1.12;
GN   Name=GAPC1; Synonyms=GAPC, GPC1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
OC   PACCAD clade; Panicoideae; Andropogoneae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Wisconsin 22;
RX   MEDLINE=90040690; PubMed=2810356; DOI=10.1016/0022-2836(89)90147-2;
RA   Martinez P., Martin W.F., Cerff R.;
RT   "Structure, evolution and anaerobic regulation of a nuclear gene
RT   encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from
RT   maize.";
RL   J. Mol. Biol. 208:551-565(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=88230473; PubMed=3131533; DOI=10.1007/BF02101150;
RA   Brinkmann H., Martinez P., Quigley F., Martin W.F., Cerff R.;
RT   "Endosymbiotic origin and codon bias of the nuclear gene for
RT   chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize.";
RL   J. Mol. Evol. 26:320-328(1987).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic
CC       form which participates in glycolysis and two chloroplast forms
CC       which participates in photosynthesis. These three forms are
CC       encoded by distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X07156; CAA30151.1; -; mRNA.
DR   EMBL; X15596; CAA33620.1; -; Genomic_DNA.
DR   PIR; S00354; DEZMGC.
DR   RefSeq; NP_001105413.1; -.
DR   UniGene; Zm.3765; -.
DR   HSSP; P56649; 1DSS.
DR   GeneID; 542367; -.
DR   Gramene; P08735; -.
DR   MaizeGDB; 13873; -.
DR   BRENDA; 1.2.1.12; 289.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:EC.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    337       Glyceraldehyde-3-phosphate dehydrogenase,
FT                                cytosolic 1.
FT                                /FTId=PRO_0000145605.
FT   NP_BIND      13     14       NAD (By similarity).
FT   REGION        1    151       Binding to NAD.
FT   REGION      152    337       Catalytic.
FT   REGION      153    155       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      213    214       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    154    154       Nucleophile (By similarity).
FT   BINDING      35     35       NAD (By similarity).
FT   BINDING      82     82       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     184    184       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     236    236       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     318    318       NAD (By similarity).
FT   SITE        181    181       Activates thiol group during catalysis
FT                                (By similarity).
FT   CONFLICT    336    336       T -> S (in Ref. 2; CAA30151).
SQ   SEQUENCE   337 AA;  36523 MW;  E18F580F09FDC07B CRC64;
     MGKIKIGING FGRIGRLVAR VALQSEDVEL VAVNDPFITT DYMTYMFKYD TVHGHWKHSD
     ITLKDSKTLL FGDKPVTVFG IRNPEEIPWG EAGAEYVVES TGVFTDKDKA AAHLKGGAKK
     VVISAPSKDA PMFVVGVNED KYTSDVNIVS NASCTTNCLA PLAKVIHDNF GIVEGLMTTV
     HAITATQKTV DGPSAKDWRG GRAASFNIIP SSTGAAKAVG KVLPDLNGKL TGMSFRVPTV
     DVSVVDLTVR IEKGASYEDI KKAIKAASEG PLKGIMGYVE EDLVSTDFLG DSRSSIFDAK
     AGIALNDHFV KLVSWYDNEW GYSNRVVDLI RHMFKTQ
//