Reviewed, UniProtKB/Swiss-Prot P08735 (G3PC_MAIZE)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, cytosolic 1
    EC=1.2.1.12

Gene names

Name:	GAPC1
Synonyms:	GAPC, GPC1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Glyceraldehyde-3-phosphate dehydrogenase, cytosolic 1		PRO_0000145605
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	1 – 151	151	Binding to NAD
	Region	152 – 337	186	Catalytic
	Region	153 – 155	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	213 – 214	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	154	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	82	1	NAD; via carbonyl oxygen By similarity
	Binding site	184	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	236	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	318	1	NAD By similarity
	Site	181	1	Activates thiol group during catalysis By similarity
Experimental info
	Sequence conflict	336	1	T → S in CAA30151. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08735-1 [UniParc]. Last modified January 1, 1990. Version 2. Checksum: E18F580F09FDC07B	FASTA	337	36,523
	10 20 30 40 50 60 MGKIKIGING FGRIGRLVAR VALQSEDVEL VAVNDPFITT DYMTYMFKYD TVHGHWKHSD 70 80 90 100 110 120 ITLKDSKTLL FGDKPVTVFG IRNPEEIPWG EAGAEYVVES TGVFTDKDKA AAHLKGGAKK 130 140 150 160 170 180 VVISAPSKDA PMFVVGVNED KYTSDVNIVS NASCTTNCLA PLAKVIHDNF GIVEGLMTTV 190 200 210 220 230 240 HAITATQKTV DGPSAKDWRG GRAASFNIIP SSTGAAKAVG KVLPDLNGKL TGMSFRVPTV 250 260 270 280 290 300 DVSVVDLTVR IEKGASYEDI KKAIKAASEG PLKGIMGYVE EDLVSTDFLG DSRSSIFDAK 310 320 330 AGIALNDHFV KLVSWYDNEW GYSNRVVDLI RHMFKTQ

References

[1]	"Structure, evolution and anaerobic regulation of a nuclear gene encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from maize." Martinez P., Martin W.F., Cerff R. J. Mol. Biol. 208:551-565(1989) [PubMed: 2810356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wisconsin 22.
[2]	"Endosymbiotic origin and codon bias of the nuclear gene for chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize." Brinkmann H., Martinez P., Quigley F., Martin W.F., Cerff R. J. Mol. Evol. 26:320-328(1987) [PubMed: 3131533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07156 mRNA. Translation: CAA30151.1. X15596 Genomic DNA. Translation: CAA33620.1.
PIR	DEZMGC. S00354.
RefSeq	NP_001105413.1.
UniGene	Zm.3765
3D structure databases
HSSP	HSSP built from PDB template 1DSS based on UniProtKB P56649.
ModBase	Search...
Genome annotation databases
GeneID	542367.
Organism-specific databases
Gramene	P08735.
MaizeGDB	13873.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PC_MAIZE

Accession

Primary (citable) accession number: P08735

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 1, 1990
Last modified:	November 25, 2008
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)