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Protein

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Gene

Myl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (PubMed:15331360). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi37 – 48Add BLAST12

GO - Molecular functioni

GO - Biological processi

  • cardiac muscle contraction Source: RGD
  • cell growth involved in cardiac muscle cell development Source: RGD
  • heart development Source: UniProtKB
  • positive regulation of the force of heart contraction Source: UniProtKB
  • regulation of the force of heart contraction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 2, ventricular/cardiac muscle isoformCurated
Short name:
MLC-2Curated
Short name:
MLC-2v
Alternative name(s):
Myosin light chain 2, slow skeletal/ventricular muscle isoformBy similarity
Short name:
MLC-2s/vBy similarity
Gene namesi
Name:Myl2Imported
Synonyms:Mlc2Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1564245. Myl2.

Subcellular locationi

GO - Cellular componenti

  • A band Source: UniProtKB-SubCell
  • contractile fiber Source: RGD
  • myofibril Source: RGD
  • myosin complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001987292 – 166Myosin regulatory light chain 2, ventricular/cardiac muscle isoformAdd BLAST165

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei52PhosphothreonineCombined sources1

Post-translational modificationi

N-terminus is methylated by METTL11A/NTM1.By similarity
Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity) (PubMed:17885681). Dephosphorylated by PPP1CB complexed to PPP1R12B (PubMed:16431080). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).By similarity2 Publications

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PRIDEiP08733.

PTM databases

iPTMnetiP08733.
PhosphoSitePlusiP08733.

Expressioni

Tissue specificityi

Abundantly expressed in both cardiac and slow skeletal muscle (soleus), with no detectable expression in fast skeletal muscle (vastus lateralis) or non-muscle tissue.1 Publication

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains (By similarity). Interacts with MYOC (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP08733.
SMRiP08733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 59EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini94 – 129EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini130 – 165EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012180.
InParanoidiP08733.
KOiK10351.
PhylomeDBiP08733.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08733-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPKKAKKRL EGGSSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR
60 70 80 90 100
DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL
110 120 130 140 150
NAFKVFDPEG KGSLKADYVR EMLTTQAERF SKEEIDQMFA AFPPDVTGNL
160
DYKNLVHIIT HGEEKD
Length:166
Mass (Da):18,880
Last modified:January 23, 2007 - v2
Checksum:i512FD14B06825275
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41D → A in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti87G → R in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti94D → G in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti99I → L in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti107 – 120DPEGK…ADYVR → APRRRITGADCVQ in AAA41621 (PubMed:3005270).CuratedAdd BLAST14
Sequence conflicti135I → T in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti145D → N in AAA41621 (PubMed:3005270).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11851 mRNA. Translation: AAA41621.1.
X07314 mRNA. Translation: CAA30277.1.
M30304
, M30298, M30299, M30300, M30301, M30302, M30303 Genomic DNA. Translation: AAA41624.1.
BC126064 mRNA. Translation: AAI26065.1.
PIRiS00752. MORT2C.
RefSeqiNP_001030329.2. NM_001035252.2.
UniGeneiRn.37176.

Genome annotation databases

GeneIDi363925.
KEGGirno:363925.
UCSCiRGD:1564245. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11851 mRNA. Translation: AAA41621.1.
X07314 mRNA. Translation: CAA30277.1.
M30304
, M30298, M30299, M30300, M30301, M30302, M30303 Genomic DNA. Translation: AAA41624.1.
BC126064 mRNA. Translation: AAI26065.1.
PIRiS00752. MORT2C.
RefSeqiNP_001030329.2. NM_001035252.2.
UniGeneiRn.37176.

3D structure databases

ProteinModelPortaliP08733.
SMRiP08733.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP08733.
PhosphoSitePlusiP08733.

Proteomic databases

PRIDEiP08733.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi363925.
KEGGirno:363925.
UCSCiRGD:1564245. rat.

Organism-specific databases

CTDi4633.
RGDi1564245. Myl2.

Phylogenomic databases

HOVERGENiHBG012180.
InParanoidiP08733.
KOiK10351.
PhylomeDBiP08733.

Miscellaneous databases

PROiP08733.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLRV_RAT
AccessioniPrimary (citable) accession number: P08733
Secondary accession number(s): A0JMZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This chain binds calcium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.