Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Gene

Myl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (PubMed:15331360). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).By similarity1 Publication

Miscellaneous

This chain binds calcium.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi37 – 48Add BLAST12

GO - Molecular functioni

GO - Biological processi

  • cardiac muscle contraction Source: RGD
  • cell growth involved in cardiac muscle cell development Source: RGD
  • heart development Source: UniProtKB
  • positive regulation of the force of heart contraction Source: UniProtKB
  • regulation of the force of heart contraction Source: UniProtKB

Keywordsi

Molecular functionMotor protein, Muscle protein, Myosin
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 2, ventricular/cardiac muscle isoformCurated
Short name:
MLC-2Curated
Short name:
MLC-2v
Alternative name(s):
Myosin light chain 2, slow skeletal/ventricular muscle isoformBy similarity
Short name:
MLC-2s/vBy similarity
Gene namesi
Name:Myl2Imported
Synonyms:Mlc2Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1564245 Myl2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001987292 – 166Myosin regulatory light chain 2, ventricular/cardiac muscle isoformAdd BLAST165

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylserineBy similarity1
Modified residuei14PhosphoserineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei52PhosphothreonineCombined sources1

Post-translational modificationi

N-terminus is methylated by METTL11A/NTM1.By similarity
Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity) (PubMed:17885681). Dephosphorylated by PPP1CB complexed to PPP1R12B (PubMed:16431080). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).By similarity2 Publications

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP08733
PRIDEiP08733

PTM databases

iPTMnetiP08733
PhosphoSitePlusiP08733

Expressioni

Tissue specificityi

Abundantly expressed in both cardiac and slow skeletal muscle (soleus), with no detectable expression in fast skeletal muscle (vastus lateralis) or non-muscle tissue.1 Publication

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains (By similarity). Interacts with MYOC (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039707

Structurei

3D structure databases

ProteinModelPortaliP08733
SMRiP08733
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 59EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini94 – 129EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini130 – 165EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012180
InParanoidiP08733
KOiK10351
PhylomeDBiP08733

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13405 EF-hand_6, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08733-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPKKAKKRL EGGSSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR
60 70 80 90 100
DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL
110 120 130 140 150
NAFKVFDPEG KGSLKADYVR EMLTTQAERF SKEEIDQMFA AFPPDVTGNL
160
DYKNLVHIIT HGEEKD
Length:166
Mass (Da):18,880
Last modified:January 23, 2007 - v2
Checksum:i512FD14B06825275
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41D → A in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti87G → R in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti94D → G in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti99I → L in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti107 – 120DPEGK…ADYVR → APRRRITGADCVQ in AAA41621 (PubMed:3005270).CuratedAdd BLAST14
Sequence conflicti135I → T in AAA41621 (PubMed:3005270).Curated1
Sequence conflicti145D → N in AAA41621 (PubMed:3005270).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11851 mRNA Translation: AAA41621.1
X07314 mRNA Translation: CAA30277.1
M30304
, M30298, M30299, M30300, M30301, M30302, M30303 Genomic DNA Translation: AAA41624.1
BC126064 mRNA Translation: AAI26065.1
PIRiS00752 MORT2C
RefSeqiNP_001030329.2, NM_001035252.2
UniGeneiRn.37176

Genome annotation databases

GeneIDi363925
KEGGirno:363925
UCSCiRGD:1564245 rat

Similar proteinsi

Entry informationi

Entry nameiMLRV_RAT
AccessioniPrimary (citable) accession number: P08733
Secondary accession number(s): A0JMZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health