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P08729 (K2C7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 162. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Keratin, type II cytoskeletal 7
Alternative name(s):
Cytokeratin-7
Short name=CK-7
Keratin-7
Short name=K7
Sarcolectin
Type-II keratin Kb7
Gene names
Name:KRT7
Synonyms:SCL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Ref.3 Ref.9

Subunit structure

Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Interacts with GPER1. Ref.8 Ref.9 Ref.15

Subcellular location

Cytoplasm Ref.2.

Tissue specificity

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. Ref.1 Ref.4

Induction

Up-regulated by retinoic acid. Ref.2

Post-translational modification

Arg-20 is dimethylated, probably to asymmetric dimethylarginine. Ref.7

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities

Belongs to the intermediate filament family.

Mass spectrometry

Molecular mass is 51203.48 Da from positions 2 - 469. Determined by MALDI. Ref.10

Sequence caution

The sequence CAA26956.2 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EIF3AQ141523EBI-297833,EBI-366617

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7
Chain2 – 469468Keratin, type II cytoskeletal 7
PRO_0000063725

Regions

Region2 – 9089Head
Region90 – 12637Coil 1A
Region91 – 399309Rod
Region92 – 976Interaction with HPV16 E7
Region127 – 14418Linker 1
Region145 – 23692Coil 1B
Region237 – 26024Linker 12
Region261 – 399139Coil 2
Region400 – 46970Tail

Sites

Site3431Stutter

Amino acid modifications

Modified residue21N-acetylserine Ref.7
Modified residue21Phosphoserine Ref.11
Modified residue201Dimethylated arginine; alternate Ref.7
Modified residue201Omega-N-methylarginine; alternate Ref.7
Modified residue1791N6-acetyllysine Ref.14
Modified residue2521Phosphoserine Ref.13
Modified residue2541Phosphoserine Ref.11

Natural variations

Natural variant1861H → R. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.16
Corresponds to variant rs6580870 [ dbSNP | Ensembl ].
VAR_060731
Natural variant3641G → A. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6
Corresponds to variant rs2608009 [ dbSNP | Ensembl ].
VAR_016321

Experimental info

Sequence conflict791D → G in CAB41416. Ref.3
Sequence conflict83 – 842SL → FS in CAB41416. Ref.3
Sequence conflict971T → A in CAA26956. Ref.1
Sequence conflict971T → A in CAA31695. Ref.2
Sequence conflict1551L → M in CAB41416. Ref.3
Sequence conflict1641A → Q in CAA26956. Ref.1
Sequence conflict1641A → Q in CAA31695. Ref.2
Sequence conflict1641A → Q in CAB41416. Ref.3
Sequence conflict1651E → G in CAA26956. Ref.1
Sequence conflict1651E → G in CAA31695. Ref.2
Sequence conflict1651E → G in CAB41416. Ref.3
Sequence conflict1681S → T in CAA26956. Ref.1
Sequence conflict1681S → T in CAA31695. Ref.2
Sequence conflict1681S → T in CAB41416. Ref.3
Sequence conflict3421R → C in CAA26956. Ref.1
Sequence conflict3421R → C in CAA31695. Ref.2
Sequence conflict4111V → A in CAB41416. Ref.3
Sequence conflict4671A → T in CAB41416. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P08729 [UniParc].

Last modified January 11, 2011. Version 5.
Checksum: 070CBE8F66A62497

FASTA46951,386
        10         20         30         40         50         60 
MSIHFSSPVF TSRSAAFSGR GAQVRLSSAR PGGLGSSSLY GLGASRPRVA VRSAYGGPVG 

        70         80         90        100        110        120 
AGIREVTINQ SLLAPLRLDA DPSLQRVRQE ESEQIKTLNN KFASFIDKVR FLEQQNKLLE 

       130        140        150        160        170        180 
TKWTLLQEQK SAKSSRLPDI FEAQIAGLRG QLEALQVDGG RLEAELRSMQ DVVEDFKNKY 

       190        200        210        220        230        240 
EDEINHRTAA ENEFVVLKKD VDAAYMSKVE LEAKVDALND EINFLRTLNE TELTELQSQI 

       250        260        270        280        290        300 
SDTSVVLSMD NSRSLDLDGI IAEVKAQYEE MAKCSRAEAE AWYQTKFETL QAQAGKHGDD 

       310        320        330        340        350        360 
LRNTRNEISE MNRAIQRLQA EIDNIKNQRA KLEAAIAEAE ERGELALKDA RAKQEELEAA 

       370        380        390        400        410        420 
LQRGKQDMAR QLREYQELMS VKLALDIEIA TYRKLLEGEE SRLAGDGVGA VNISVMNSTG 

       430        440        450        460 
GSSSGGGIGL TLGGTMGSNA LSFSSSAGPG LLKAYSIRTA SASRRSARD 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and expression of a human type II mesothelial keratin."
Glass C., Kim K.H., Fuchs E.
J. Cell Biol. 101:2366-2373(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANTS ARG-186 AND ALA-364.
Tissue: Mesothelium.
[2]"Isolation, sequence, and differential expression of a human K7 gene in simple epithelial cells."
Glass C., Fuchs E.
J. Cell Biol. 107:1337-1350(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, INDUCTION, VARIANTS ARG-186 AND ALA-364.
[3]"Sarcolectin (SCL): structure and expression of the recombinant molecule."
Kaba A., Jiang P., Chany-Fournier F., Chany C.
Biochimie 81:709-715(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANTS ARG-186 AND ALA-364.
Tissue: Placenta.
[4]"Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse."
Smith F.J.D., Porter R.M., Corden L.D., Lunny D.P., Lane E.B., McLean W.H.I.
Biochem. Biophys. Res. Commun. 297:818-827(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, VARIANTS ARG-186 AND ALA-364.
Tissue: Keratinocyte.
[5]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ARG-186 AND ALA-364.
Tissue: Pancreas.
[7]Bienvenut W.V., Zebisch A., Lilla S., von Kriegsheim A., Lempens A., Kolch W.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-48; 53-96; 102-110; 123-130; 137-161; 178-208; 215-273; 277-296; 306-313; 318-326; 330-348; 352-363 AND 374-402, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, METHYLATION AT ARG-20, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Colon carcinoma and Ovarian carcinoma.
[8]"Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7."
Lin L., Holbro T., Alonso G., Gerosa D., Burger M.M.
J. Cell. Biochem. 80:483-490(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EIF3S10.
[9]"Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit alpha(1)-globin and human cytokeratin 7."
Kanduc D.
J. Virol. 76:7040-7048(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HPV16 E7.
[10]"Cluster analysis of an extensive human breast cancer cell line protein expression map database."
Harris R.A., Yang A., Stein R.C., Lucy K., Brusten L., Herath A., Parekh R., Waterfield M.D., O'Hare M.J., Neville M.A., Page M.J., Zvelebil M.J.
Proteomics 2:212-223(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
Tissue: Mammary cancer.
[11]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-179, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"G protein-coupled estrogen receptor 1/G protein-coupled receptor 30 localizes in the plasma membrane and traffics intracellularly on cytokeratin intermediate filaments."
Sanden C., Broselid S., Cornmark L., Andersson K., Daszkiewicz-Nilsson J., Martensson U.E., Olde B., Leeb-Lundberg L.M.
Mol. Pharmacol. 79:400-410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GPER1.
[16]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-186, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03212 mRNA. Translation: CAA26956.2. Sequence problems.
X13320 expand/collapse EMBL AC list , X13346, X13347, X13348, X13349, X13350, X13351, X13352, X13353 Genomic DNA. Translation: CAA31695.1.
AJ238246 mRNA. Translation: CAB41416.1.
AF509887 mRNA. Translation: AAN64031.1.
AF509892, AF509891 Genomic DNA. Translation: AAN64035.1.
AC007338 Genomic DNA. No translation available.
AC007494 Genomic DNA. No translation available.
BC002700 mRNA. Translation: AAH02700.1.
CCDSCCDS8822.1.
PIRB24177.
S05602.
RefSeqNP_005547.3. NM_005556.3.
UniGeneHs.411501.
Hs.670221.

3D structure databases

ProteinModelPortalP08729.
SMRP08729. Positions 88-240, 256-398.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110053. 10 interactions.
IntActP08729. 6 interactions.
MINTMINT-256895.
STRING9606.ENSP00000329243.

PTM databases

PhosphoSiteP08729.

Polymorphism databases

DMDM317373583.

2D gel databases

SWISS-2DPAGEP08729.

Proteomic databases

MaxQBP08729.
PaxDbP08729.
PRIDEP08729.

Protocols and materials databases

DNASU3855.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000331817; ENSP00000329243; ENSG00000135480.
GeneID3855.
KEGGhsa:3855.
UCSCuc001saa.1. human.

Organism-specific databases

CTD3855.
GeneCardsGC12P052626.
H-InvDBHIX0010643.
HIX0079487.
HGNCHGNC:6445. KRT7.
HPACAB000028.
HPA007272.
MIM148059. gene.
neXtProtNX_P08729.
PharmGKBPA30233.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG145985.
HOGENOMHOG000230976.
HOVERGENHBG013015.
InParanoidP08729.
KOK07605.
OMAMSIHFSS.
OrthoDBEOG7FV3Q8.
PhylomeDBP08729.
TreeFamTF317854.

Gene expression databases

BgeeP08729.
CleanExHS_KRT7.
GenevestigatorP08729.

Family and domain databases

InterProIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR003054. Keratin_II.
IPR009053. Prefoldin.
[Graphical view]
PANTHERPTHR23239. PTHR23239. 1 hit.
PfamPF00038. Filament. 1 hit.
[Graphical view]
PRINTSPR01276. TYPE2KERATIN.
SUPFAMSSF46579. SSF46579. 1 hit.
PROSITEPS00226. IF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKRT7. human.
GeneWikiKeratin_7.
GenomeRNAi3855.
NextBio15169.
PROP08729.
SOURCESearch...

Entry information

Entry nameK2C7_HUMAN
AccessionPrimary (citable) accession number: P08729
Secondary accession number(s): Q92676, Q9BUD8, Q9Y3R7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 162 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM