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Protein

PTS system beta-glucoside-specific EIIBCA component

Gene

bglF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in beta-glucoside transport.
Acts as both a kinase and a phosphatase on BglG.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei24Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation1
Active sitei547Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:BGLF-MONOMER
MetaCyc:BGLF-MONOMER

Protein family/group databases

TCDBi4.A.1.2.2 the pts glucose-glucoside (glc) family

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system beta-glucoside-specific EIIBCA component
Alternative name(s):
EIIBCA-Bgl
Short name:
EII-Bgl
Including the following 3 domains:
Beta-glucoside-specific phosphotransferase enzyme IIB component (EC:2.7.1.-)
Alternative name(s):
PTS system beta-glucoside-specific EIIB component
Beta-glucoside permease IIC component
Alternative name(s):
PTS system beta-glucoside-specific EIIC component
Beta-glucoside-specific phosphotransferase enzyme IIA component
Alternative name(s):
PTS system beta-glucoside-specific EIIA component
Gene namesi
Name:bglF
Synonyms:bglC, bglS
Ordered Locus Names:b3722, JW3700
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10115 bglF

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 99PeriplasmicSequence analysisAdd BLAST99
Transmembranei100 – 120HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini121 – 140CytoplasmicSequence analysisAdd BLAST20
Transmembranei141 – 161HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini162 – 166PeriplasmicSequence analysis5
Transmembranei167 – 187HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini188 – 202CytoplasmicSequence analysisAdd BLAST15
Transmembranei203 – 223HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini224 – 244PeriplasmicSequence analysisAdd BLAST21
Transmembranei245 – 265HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini266 – 284CytoplasmicSequence analysisAdd BLAST19
Transmembranei285 – 305HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini306 – 324PeriplasmicSequence analysisAdd BLAST19
Transmembranei325 – 345HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini346 – 353CytoplasmicSequence analysis8
Transmembranei354 – 374HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini375 – 380PeriplasmicSequence analysis6
Transmembranei381 – 401HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini402 – 403CytoplasmicSequence analysis2
Transmembranei404 – 424HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini425 – 431PeriplasmicSequence analysis7
Transmembranei432 – 452HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini453 – 625CytoplasmicSequence analysisAdd BLAST173

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864811 – 625PTS system beta-glucoside-specific EIIBCA componentAdd BLAST625

Proteomic databases

PaxDbiP08722
PRIDEiP08722

Interactioni

Protein-protein interaction databases

BioGridi4262137, 5 interactors
DIPiDIP-9215N
IntActiP08722, 1 interactor
STRINGi316385.ECDH10B_3909

Structurei

3D structure databases

ProteinModelPortaliP08722
SMRiP08722
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 84PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST84
Domaini102 – 465PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST364
Domaini495 – 599PTS EIIA type-1PROSITE-ProRule annotationAdd BLAST105

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C5Y Bacteria
COG1263 LUCA
COG1264 LUCA
COG2190 LUCA
HOGENOMiHOG000102023
InParanoidiP08722
KOiK02755
K02756
K02757
OMAiLWQVCVI
PhylomeDBiP08722

Family and domain databases

CDDicd00212 PTS_IIB_glc, 1 hit
Gene3Di3.30.1360.60, 1 hit
InterProiView protein in InterPro
IPR011055 Dup_hybrid_motif
IPR036878 Glu_permease_IIB
IPR018113 PTrfase_EIIB_Cys
IPR001127 PTS_EIIA_1_perm
IPR003352 PTS_EIIC
IPR013013 PTS_EIIC_1
IPR011297 PTS_IIABC_b_glu
IPR001996 PTS_IIB_1
IPR004719 PTS_maltose/Glc_sub_IIC
PfamiView protein in Pfam
PF00358 PTS_EIIA_1, 1 hit
PF00367 PTS_EIIB, 1 hit
PF02378 PTS_EIIC, 1 hit
SUPFAMiSSF51261 SSF51261, 1 hit
SSF55604 SSF55604, 1 hit
TIGRFAMsiTIGR00826 EIIB_glc, 1 hit
TIGR00830 PTBA, 1 hit
TIGR00852 pts-Glc, 1 hit
TIGR01995 PTS-II-ABC-beta, 1 hit
PROSITEiView protein in PROSITE
PS51093 PTS_EIIA_TYPE_1, 1 hit
PS00371 PTS_EIIA_TYPE_1_HIS, 1 hit
PS51098 PTS_EIIB_TYPE_1, 1 hit
PS01035 PTS_EIIB_TYPE_1_CYS, 1 hit
PS51103 PTS_EIIC_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P08722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELARKIVA GVGGADNIVS LMHCATRLRF KLKDESKAQA EVLKKTPGII
60 70 80 90 100
MVVESGGQFQ VVIGNHVADV FLAVNSVAGL DEKAQQAPEN DDKGNLLNRF
110 120 130 140 150
VYVISGIFTP LIGLMAATGI LKGMLALALT FQWTTEQSGT YLILFSASDA
160 170 180 190 200
LFWFFPIILG YTAGKRFGGN PFTAMVIGGA LVHPLILTAF ENGQKADALG
210 220 230 240 250
LDFLGIPVTL LNYSSSVIPI IFSAWLCSIL ERRLNAWLPS AIKNFFTPLL
260 270 280 290 300
CLMVITPVTF LLVGPLSTWI SELIAAGYLW LYQAVPAFAG AVMGGFWQIF
310 320 330 340 350
VMFGLHWGLV PLCINNFTVL GYDTMIPLLM PAIMAQVGAA LGVFLCERDA
360 370 380 390 400
QKKVVAGSAA LTSLFGITEP AVYGVNLPRK YPFVIACISG ALGATIIGYA
410 420 430 440 450
QTKVYSFGLP SIFTFMQTIP STGIDFTVWA SVIGGVIAIG CAFVGTVMLH
460 470 480 490 500
FITAKRQPAQ GAPQEKTPEV ITPPEQGGIC SPMTGEIVPL IHVADTTFAS
510 520 530 540 550
GLLGKGIAIL PSVGEVRSPV AGRIASLFAT LHAIGIESDD GVEILIHVGI
560 570 580 590 600
DTVKLDGKFF SAHVNVGDKV NTGDRLISFD IPAIREAGFD LTTPVLISNS
610 620
DDFTDVLPHG TAQISAGEPL LSIIR
Length:625
Mass (Da):66,483
Last modified:August 1, 1988 - v1
Checksum:i9E12A2207125C4E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15746 Genomic DNA Translation: AAA83837.1
M16487 Genomic DNA Translation: AAA23510.1
L10328 Genomic DNA Translation: AAA62073.1
U00096 Genomic DNA Translation: AAC76745.1
AP009048 Genomic DNA Translation: BAE77566.1
PIRiC25977
RefSeqiNP_418178.1, NC_000913.3
WP_000137296.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76745; AAC76745; b3722
BAE77566; BAE77566; BAE77566
GeneIDi948236
KEGGiecj:JW3700
eco:b3722
PATRICifig|1411691.4.peg.2979

Similar proteinsi

Entry informationi

Entry nameiPTV3B_ECOLI
AccessioniPrimary (citable) accession number: P08722
Secondary accession number(s): Q2M840
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: March 28, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health