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Protein

PTS system beta-glucoside-specific EIIBCA component

Gene

bglF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in beta-glucoside transport.
Acts as both a kinase and a phosphatase on BglG.

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation
Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei24Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation1
Active sitei547Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:BGLF-MONOMER.
ECOL316407:JW3700-MONOMER.
MetaCyc:BGLF-MONOMER.

Protein family/group databases

TCDBi4.A.1.2.2. the pts glucose-glucoside (glc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system beta-glucoside-specific EIIBCA component
Alternative name(s):
EIIBCA-Bgl
Short name:
EII-Bgl
Including the following 3 domains:
Beta-glucoside-specific phosphotransferase enzyme IIB component (EC:2.7.1.-)
Alternative name(s):
PTS system beta-glucoside-specific EIIB component
Beta-glucoside permease IIC component
Alternative name(s):
PTS system beta-glucoside-specific EIIC component
Beta-glucoside-specific phosphotransferase enzyme IIA component
Alternative name(s):
PTS system beta-glucoside-specific EIIA component
Gene namesi
Name:bglF
Synonyms:bglC, bglS
Ordered Locus Names:b3722, JW3700
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10115. bglF.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 99PeriplasmicSequence analysisAdd BLAST99
Transmembranei100 – 120HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini121 – 140CytoplasmicSequence analysisAdd BLAST20
Transmembranei141 – 161HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini162 – 166PeriplasmicSequence analysis5
Transmembranei167 – 187HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini188 – 202CytoplasmicSequence analysisAdd BLAST15
Transmembranei203 – 223HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini224 – 244PeriplasmicSequence analysisAdd BLAST21
Transmembranei245 – 265HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini266 – 284CytoplasmicSequence analysisAdd BLAST19
Transmembranei285 – 305HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini306 – 324PeriplasmicSequence analysisAdd BLAST19
Transmembranei325 – 345HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini346 – 353CytoplasmicSequence analysis8
Transmembranei354 – 374HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini375 – 380PeriplasmicSequence analysis6
Transmembranei381 – 401HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini402 – 403CytoplasmicSequence analysis2
Transmembranei404 – 424HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini425 – 431PeriplasmicSequence analysis7
Transmembranei432 – 452HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini453 – 625CytoplasmicSequence analysisAdd BLAST173

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864811 – 625PTS system beta-glucoside-specific EIIBCA componentAdd BLAST625

Proteomic databases

PaxDbiP08722.
PRIDEiP08722.

Interactioni

Protein-protein interaction databases

BioGridi4262137. 5 interactors.
DIPiDIP-9215N.
IntActiP08722. 1 interactor.
STRINGi511145.b3722.

Structurei

3D structure databases

ProteinModelPortaliP08722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 84PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST84
Domaini102 – 465PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST364
Domaini495 – 599PTS EIIA type-1PROSITE-ProRule annotationAdd BLAST105

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C5Y. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
COG2190. LUCA.
HOGENOMiHOG000102023.
InParanoidiP08722.
KOiK02755.
K02756.
K02757.
OMAiKIWFAAS.
PhylomeDBiP08722.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR011297. PTS_IIABC_b_glu.
IPR001996. PTS_IIB_1.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR01995. PTS-II-ABC-beta. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELARKIVA GVGGADNIVS LMHCATRLRF KLKDESKAQA EVLKKTPGII
60 70 80 90 100
MVVESGGQFQ VVIGNHVADV FLAVNSVAGL DEKAQQAPEN DDKGNLLNRF
110 120 130 140 150
VYVISGIFTP LIGLMAATGI LKGMLALALT FQWTTEQSGT YLILFSASDA
160 170 180 190 200
LFWFFPIILG YTAGKRFGGN PFTAMVIGGA LVHPLILTAF ENGQKADALG
210 220 230 240 250
LDFLGIPVTL LNYSSSVIPI IFSAWLCSIL ERRLNAWLPS AIKNFFTPLL
260 270 280 290 300
CLMVITPVTF LLVGPLSTWI SELIAAGYLW LYQAVPAFAG AVMGGFWQIF
310 320 330 340 350
VMFGLHWGLV PLCINNFTVL GYDTMIPLLM PAIMAQVGAA LGVFLCERDA
360 370 380 390 400
QKKVVAGSAA LTSLFGITEP AVYGVNLPRK YPFVIACISG ALGATIIGYA
410 420 430 440 450
QTKVYSFGLP SIFTFMQTIP STGIDFTVWA SVIGGVIAIG CAFVGTVMLH
460 470 480 490 500
FITAKRQPAQ GAPQEKTPEV ITPPEQGGIC SPMTGEIVPL IHVADTTFAS
510 520 530 540 550
GLLGKGIAIL PSVGEVRSPV AGRIASLFAT LHAIGIESDD GVEILIHVGI
560 570 580 590 600
DTVKLDGKFF SAHVNVGDKV NTGDRLISFD IPAIREAGFD LTTPVLISNS
610 620
DDFTDVLPHG TAQISAGEPL LSIIR
Length:625
Mass (Da):66,483
Last modified:August 1, 1988 - v1
Checksum:i9E12A2207125C4E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15746 Genomic DNA. Translation: AAA83837.1.
M16487 Genomic DNA. Translation: AAA23510.1.
L10328 Genomic DNA. Translation: AAA62073.1.
U00096 Genomic DNA. Translation: AAC76745.1.
AP009048 Genomic DNA. Translation: BAE77566.1.
PIRiC25977.
RefSeqiNP_418178.1. NC_000913.3.
WP_000137296.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76745; AAC76745; b3722.
BAE77566; BAE77566; BAE77566.
GeneIDi948236.
KEGGiecj:JW3700.
eco:b3722.
PATRICi32122939. VBIEscCol129921_3845.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15746 Genomic DNA. Translation: AAA83837.1.
M16487 Genomic DNA. Translation: AAA23510.1.
L10328 Genomic DNA. Translation: AAA62073.1.
U00096 Genomic DNA. Translation: AAC76745.1.
AP009048 Genomic DNA. Translation: BAE77566.1.
PIRiC25977.
RefSeqiNP_418178.1. NC_000913.3.
WP_000137296.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP08722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262137. 5 interactors.
DIPiDIP-9215N.
IntActiP08722. 1 interactor.
STRINGi511145.b3722.

Protein family/group databases

TCDBi4.A.1.2.2. the pts glucose-glucoside (glc) family.

Proteomic databases

PaxDbiP08722.
PRIDEiP08722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76745; AAC76745; b3722.
BAE77566; BAE77566; BAE77566.
GeneIDi948236.
KEGGiecj:JW3700.
eco:b3722.
PATRICi32122939. VBIEscCol129921_3845.

Organism-specific databases

EchoBASEiEB0113.
EcoGeneiEG10115. bglF.

Phylogenomic databases

eggNOGiENOG4105C5Y. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
COG2190. LUCA.
HOGENOMiHOG000102023.
InParanoidiP08722.
KOiK02755.
K02756.
K02757.
OMAiKIWFAAS.
PhylomeDBiP08722.

Enzyme and pathway databases

BioCyciEcoCyc:BGLF-MONOMER.
ECOL316407:JW3700-MONOMER.
MetaCyc:BGLF-MONOMER.

Miscellaneous databases

PROiP08722.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR011297. PTS_IIABC_b_glu.
IPR001996. PTS_IIB_1.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR01995. PTS-II-ABC-beta. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTV3B_ECOLI
AccessioniPrimary (citable) accession number: P08722
Secondary accession number(s): Q2M840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.