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Protein

Osteopontin

Gene

Spp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.By similarity

GO - Molecular functioni

GO - Biological processi

  • biomineral tissue development Source: UniProtKB-KW
  • cell adhesion Source: RGD
  • cell differentiation Source: RGD
  • inflammatory response Source: RGD
  • negative regulation of collateral sprouting of intact axon in response to injury Source: RGD
  • osteoblast differentiation Source: RGD
  • positive regulation of bone resorption Source: RGD
  • response to organic substance Source: RGD
  • response to steroid hormone Source: RGD
  • response to vitamin D Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Biomineralization, Cell adhesion

Keywords - Ligandi

Sialic acid

Enzyme and pathway databases

ReactomeiR-RNO-1474228. Degradation of the extracellular matrix.
R-RNO-186797. Signaling by PDGF.
R-RNO-216083. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Osteopontin
Alternative name(s):
Bone sialoprotein 1
Secreted phosphoprotein 1
Short name:
SPP-1
Gene namesi
Name:Spp1
Synonyms:2b7, Spp-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi3752. Spp1.

Subcellular locationi

GO - Cellular componenti

  • cell projection Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: RGD
  • membrane-bounded vesicle Source: RGD
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 163 PublicationsAdd BLAST16
ChainiPRO_000002032517 – 317OsteopontinAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineBy similarity1
Modified residuei27PhosphoserineBy similarity1
Modified residuei60PhosphoserineBy similarity1
Modified residuei62PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Modified residuei66PhosphothreonineBy similarity1
Modified residuei76PhosphoserineBy similarity1
Modified residuei78PhosphoserineBy similarity1
Glycosylationi79N-linked (GlcNAc...)Sequence analysis1
Modified residuei81PhosphoserineBy similarity1
Modified residuei106PhosphoserineBy similarity1
Modified residuei109PhosphoserineBy similarity1
Modified residuei112PhosphoserineBy similarity1
Modified residuei115PhosphoserineBy similarity1
Modified residuei118PhosphoserineBy similarity1
Glycosylationi123O-linked (GalNAc...)By similarity1
Glycosylationi132O-linked (GalNAc...)By similarity1
Glycosylationi137O-linked (GalNAc...)By similarity1
Modified residuei170PhosphothreonineBy similarity1
Modified residuei175PhosphothreonineBy similarity1
Modified residuei176PhosphoserineBy similarity1
Modified residuei180PhosphoserineBy similarity1
Modified residuei200PhosphoserineBy similarity1
Modified residuei204PhosphoserineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei219PhosphoserineBy similarity1
Modified residuei222PhosphothreonineBy similarity1
Modified residuei224PhosphoserineBy similarity1
Modified residuei228PhosphoserineBy similarity1
Modified residuei257PhosphoserineBy similarity1
Modified residuei261PhosphoserineBy similarity1
Modified residuei266PhosphoserineBy similarity1
Modified residuei270PhosphoserineBy similarity1
Modified residuei273PhosphoserineBy similarity1
Modified residuei278PhosphoserineBy similarity1
Modified residuei283PhosphoserineBy similarity1
Modified residuei294PhosphoserineBy similarity1
Modified residuei306PhosphoserineBy similarity1
Modified residuei311PhosphoserineBy similarity1
Modified residuei313PhosphoserineBy similarity1
Modified residuei314PhosphoserineBy similarity1

Post-translational modificationi

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.By similarity
N- and O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08721.
PRIDEiP08721.

PTM databases

iPTMnetiP08721.
PhosphoSitePlusiP08721.

Expressioni

Gene expression databases

BgeeiENSRNOG00000043451.
GenevisibleiP08721. RN.

Interactioni

Subunit structurei

Ligand for integrin alpha-V/beta-3.

Protein-protein interaction databases

BioGridi247392. 4 interactors.
IntActiP08721. 2 interactors.
MINTiMINT-8283233.
STRINGi10116.ENSRNOP00000062358.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi144 – 146Cell attachment site3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi86 – 96Poly-AspAdd BLAST11

Sequence similaritiesi

Belongs to the osteopontin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
GeneTreeiENSGT00390000002509.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP08721.
KOiK06250.
OMAiVICFCLL.
OrthoDBiEOG091G0KVC.
PhylomeDBiP08721.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 2 hits.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08721-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLAVVCFCL FGLASCLPVK VAEFGSSEEK AHYSKHSDAV ATWLKPDPSQ
60 70 80 90 100
KQNLLAPQNS VSSEETDDFK QETLPSNSNE SHDHMDDDDD DDDDGDHAES
110 120 130 140 150
EDSVNSDESD ESHHSDESDE SFTASTQADV LTPIAPTVDV PDGRGDSLAY
160 170 180 190 200
GLRSKSRSFP VSDEQYPDAT DEDLTSRMKS QESDEAIKVI PVAQRLSVPS
210 220 230 240 250
DQDSNGKTSH ESSQLDEPSV ETHSLEQSKE YKQRASHEST EQSDAIDSAE
260 270 280 290 300
KPDAIDSAER SDAIDSQASS KASLEHQSHE FHSHEDKLVL DPKSKEDDRY
310
LKFRISHELE SSSSEVN
Length:317
Mass (Da):34,963
Last modified:April 1, 1993 - v2
Checksum:i73CB5C21FFF62310
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8F → L in AAA41762 (PubMed:3024151).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99252 mRNA. Translation: AAA41765.1.
M14656 mRNA. Translation: AAA41762.1.
BC078874 mRNA. Translation: AAH78874.1.
PIRiA25917.
JC5811.
RefSeqiNP_037013.2. NM_012881.2.
XP_008768218.1. XM_008769996.2.
UniGeneiRn.8871.

Genome annotation databases

EnsembliENSRNOT00000067875; ENSRNOP00000062358; ENSRNOG00000043451.
ENSRNOT00000075989; ENSRNOP00000068511; ENSRNOG00000043451.
GeneIDi25353.
KEGGirno:25353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99252 mRNA. Translation: AAA41765.1.
M14656 mRNA. Translation: AAA41762.1.
BC078874 mRNA. Translation: AAH78874.1.
PIRiA25917.
JC5811.
RefSeqiNP_037013.2. NM_012881.2.
XP_008768218.1. XM_008769996.2.
UniGeneiRn.8871.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247392. 4 interactors.
IntActiP08721. 2 interactors.
MINTiMINT-8283233.
STRINGi10116.ENSRNOP00000062358.

PTM databases

iPTMnetiP08721.
PhosphoSitePlusiP08721.

Proteomic databases

PaxDbiP08721.
PRIDEiP08721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067875; ENSRNOP00000062358; ENSRNOG00000043451.
ENSRNOT00000075989; ENSRNOP00000068511; ENSRNOG00000043451.
GeneIDi25353.
KEGGirno:25353.

Organism-specific databases

CTDi6696.
RGDi3752. Spp1.

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
GeneTreeiENSGT00390000002509.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP08721.
KOiK06250.
OMAiVICFCLL.
OrthoDBiEOG091G0KVC.
PhylomeDBiP08721.

Enzyme and pathway databases

ReactomeiR-RNO-1474228. Degradation of the extracellular matrix.
R-RNO-186797. Signaling by PDGF.
R-RNO-216083. Integrin cell surface interactions.

Miscellaneous databases

PROiP08721.

Gene expression databases

BgeeiENSRNOG00000043451.
GenevisibleiP08721. RN.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 2 hits.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSTP_RAT
AccessioniPrimary (citable) accession number: P08721
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.