P08703 (IPNS_PENCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isopenicillin N synthase
Short name=IPNS
EC=1.21.3.1

Gene names

Name:	pcbC
Synonyms:	ips

Organism

Penicillium chrysogenum (Penicillium notatum)

Taxonomic identifier

5076 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium › Penicillium chrysogenum complex

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.
Catalytic activity	N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.
Cofactor	Iron. Ascorbate.
Pathway	Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 2/3.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro isopenicillin-N synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Isopenicillin N synthase		PRO_0000219503
Regions
	Domain	176 – 288	113	Fe2OG dioxygenase
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	216	1	Iron By similarity
	Metal binding	270	1	Iron By similarity
Natural variations
	Natural variant	195	1	I → Y in strain: AS-P-78.

Sequences

	Sequence		Length	Mass (Da)
	P08703 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: E338FACBC7C74FC4	FASTA	331	37,960
	10 20 30 40 50 60 MASTPKANVP KIDVSPLFGD NMEEKMKVAR AIDAASRDTG FFYAVNHGVD VKRLSNKTRE 70 80 90 100 110 120 FHFSITDEEK WDLAIRAYNK EHQDQIRAGY YLSIPEKKAV ESFCYLNPNF KPDHPLIQSK 130 140 150 160 170 180 TPTHEVNVWP DEKKHPGFRE FAEQYYWDVF GLSSALLRGY ALALGKEEDF FSRHFKKEDA 190 200 210 220 230 240 LSSVVLIRYP YLNPIPPAAI KTAEDGTKLS FEWHEDVSLI TVLYQSDVAN LQVEMPQGYL 250 260 270 280 290 300 DIEADDNAYL VNCGSYMAHI TNNYYPAPIH RVKWVNEERQ SLPFFVNLGF NDTVQPWDPS 310 320 330 KEDGKTDQRP ISYGDYLQNG LVSLINKNGQ T

References

[1]	"Cloning and expression of the isopenicillin N synthetase gene from Penicillium chrysogenum." Carr L.G., Skatrud P.L., Scheetz M.E. II, Queener S.W., Ingolia T.D. Gene 48:257-266(1986) [PubMed: 3104145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 23X-80-269-37-2.
[2]	"Cloning, sequence analysis and transcriptional study of the isopenicillin N synthase of Penicillium chrysogenum AS-P-78." Barrredo J.L., Cantoral J.M., Alvarez E., Diez B., Martin J.F. Mol. Gen. Genet. 216:91-98(1989) [PubMed: 2499766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AS-P-78.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15083 Genomic DNA. Translation: AAA33696.1. X17436 Genomic DNA. Translation: CAA35480.1.
PIR	A26467. S04441.
3D structure databases
ProteinModelPortal	P08703.
SMR	P08703. Positions 3-331.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002057. Isopenicillin-N_synth_CS. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. PS00185. IPNS_1. 1 hit. PS00186. IPNS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPNS_PENCH

Accession

Primary (citable) accession number: P08703

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	October 19, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program