Reviewed, UniProtKB/Swiss-Prot P08703 (IPNS_PENCH)

Names and origin

Protein names

Recommended name:
    Isopenicillin N synthetase
    EC=1.21.3.1
Alternative name(s):
    Isopenicillin N synthase
      Short name=IPNS

Gene names

Name:	pcbC
Synonyms:	ips

Organism

Penicillium chrysogenum (Penicillium notatum)

Taxonomic identifier

5076 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium › Penicillium chrysogenum complex

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.
Catalytic activity	N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.
Cofactor	Iron. Ascorbate.
Pathway	Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 2/3.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW isopenicillin-N synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Isopenicillin N synthetase		PRO_0000219503
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	216	1	Iron By similarity
	Metal binding	270	1	Iron By similarity
Natural variations
	Natural variant	195	1	I → Y in strain: AS-P-78.

Sequences

	Sequence		Length	Mass (Da)
	P08703-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: E338FACBC7C74FC4	FASTA	331	37,960
	10 20 30 40 50 60 MASTPKANVP KIDVSPLFGD NMEEKMKVAR AIDAASRDTG FFYAVNHGVD VKRLSNKTRE 70 80 90 100 110 120 FHFSITDEEK WDLAIRAYNK EHQDQIRAGY YLSIPEKKAV ESFCYLNPNF KPDHPLIQSK 130 140 150 160 170 180 TPTHEVNVWP DEKKHPGFRE FAEQYYWDVF GLSSALLRGY ALALGKEEDF FSRHFKKEDA 190 200 210 220 230 240 LSSVVLIRYP YLNPIPPAAI KTAEDGTKLS FEWHEDVSLI TVLYQSDVAN LQVEMPQGYL 250 260 270 280 290 300 DIEADDNAYL VNCGSYMAHI TNNYYPAPIH RVKWVNEERQ SLPFFVNLGF NDTVQPWDPS 310 320 330 KEDGKTDQRP ISYGDYLQNG LVSLINKNGQ T

References

[1]	"Cloning and expression of the isopenicillin N synthetase gene from Penicillium chrysogenum." Carr L.G., Skatrud P.L., Scheetz M.E. II, Queener S.W., Ingolia T.D. Gene 48:257-266(1986) [PubMed: 3104145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 23X-80-269-37-2.
[2]	"Cloning, sequence analysis and transcriptional study of the isopenicillin N synthase of Penicillium chrysogenum AS-P-78." Barrredo J.L., Cantoral J.M., Alvarez E., Diez B., Martin J.F. Mol. Gen. Genet. 216:91-98(1989) [PubMed: 2499766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AS-P-78.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15083 Genomic DNA. Translation: AAA33696.1. X17436 Genomic DNA. Translation: CAA35480.1.
PIR	A26467. S04441.
3D structure databases
HSSP	HSSP built from PDB template 1QJE based on UniProtKB P05326.
SMR	P08703. Positions 3-331.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.21.3.1. 285.
Family and domain databases
InterPro	IPR002057. Isopenicillin-N_synth_CS. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS00185. IPNS_1. 1 hit. PS00186. IPNS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPNS_PENCH

Accession

Primary (citable) accession number: P08703

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)