P08700 (IL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-3
Short name=IL-3

Alternative name(s):
Hematopoietic growth factor
Mast cell growth factor
Short name=MCGF
Multipotential colony-stimulating factor
P-cell-stimulating factor

Gene names

Name:

IL3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Subunit structure	Monomer.
Subcellular location	Secreted.
Tissue specificity	Activated T-cells, mast cells, natural killer cells.
Sequence similarities	Belongs to the IL-3 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement PubMed 3497400. Source: ProtInc embryonic hemopoiesis Inferred from direct assay PubMed 21149635. Source: DFLAT immune response Inferred from electronic annotation. Source: InterPro nervous system development Traceable author statement PubMed 9736740. Source: ProtInc positive regulation of DNA replication Inferred from direct assay PubMed 9722506. Source: BHF-UCL positive regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of tyrosine phosphorylation of Stat5 protein Inferred from direct assay PubMed 9722506. Source: BHF-UCL
Cellular_component	extracellular space Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	interleukin-3 receptor binding Traceable author statement Ref.7. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CSF2RB	P32927	1	EBI-1811718,EBI-1809771

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Chain

20 – 152

133

Interleukin-3

PRO_0000015516

Amino acid modifications

Glycosylation

34

1

N-linked (GlcNAc...) Potential

Glycosylation

89

1

N-linked (GlcNAc...) Potential

Disulfide bond

35 ↔ 103

Natural variations

Natural variant

3

1

R → C.
Corresponds to variant rs35415145 [ dbSNP | Ensembl ].

VAR_034014

Natural variant

15

1

R → H. Ref.4
Corresponds to variant rs2069787 [ dbSNP | Ensembl ].

VAR_013071

Natural variant

27

1

P → S. Ref.4 Ref.6 Ref.7
Corresponds to variant rs40401 [ dbSNP | Ensembl ].

VAR_013072

Natural variant

60

1

N → S.
Corresponds to variant rs35482671 [ dbSNP | Ensembl ].

VAR_034015

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

152

Helix Strand Turn

Details...

Helix

35 – 45

11

Helix

61 – 68

8

Helix

73 – 86

14

Helix

91 – 100

10

Helix

101 – 103

3

Helix

123 – 141

19

Sequences

	Sequence		Length	Mass (Da)
	P08700 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: BB46F23FAC1259A4	FASTA	152	17,233
	10 20 30 40 50 60 MSRLPVLLLL QLLVRPGLQA PMTQTTPLKT SWVNCSNMID EIITHLKQPP LPLLDFNNLN 70 80 90 100 110 120 GEDQDILMEN NLRRPNLEAF NRAVKSLQNA SAIESILKNL LPCLPLATAA PTRHPIHIKD 130 140 150 GDWNEFRRKL TFYLKTLENA QAQQTTLSLA IF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a human multilineage-colony-stimulating factor cDNA clone identified by a conserved noncoding sequence in mouse interleukin-3." Dorssers L., Burger H., Bot F., Delwel R., Geurts van Kessel A.H.M., Loewenberg B., Wagemaker G. Gene 55:115-124(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and characterization of an expressible cDNA encoding human IL-3. Induction of IL-3 mRNA in human T cell clones." Otsuka T., Miyajima A., Brown N., Otsu K., Abrams J., Saeland S., Caux C., de Waal Malefijt R., de Vries J., Meyerson P., Yokota K., Gemmel L., Rennick D., Lee F., Arai N., Arai K., Yokota T. J. Immunol. 140:2288-2295(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3." Yang Y.-C., Ciarletta A.B., Temple P.A., Chung M.P., Kovacic S., Witek-Giannotti J.S., Leary A.C., Kriz R., Donahue R.E., Wong G.G., Clark S.C. Cell 47:3-10(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	SeattleSNPs variation discovery resource Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-15 AND SER-27.
[5]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-27.
[7]	"Molecular characterization of colony-stimulating factors and their receptors: human interleukin-3." Urdal D.L., Price V., Sassenfeld H.M., Cosman D., Gillis S., Park L.S. Ann. N. Y. Acad. Sci. 554:167-176(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 20-152, VARIANT SER-27.
[8]	"Three-dimensional solution structure and backbone dynamics of a variant of human interleukin-3." Feng Y., Klein B.K., McWherter C.A. J. Mol. Biol. 259:524-541(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

SeattleSNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M14743 mRNA. Translation: AAA59146.1.
M20137 mRNA. Translation: AAA59147.1.
M17115 mRNA. Translation: AAA35725.1.
AF365976 Genomic DNA. Translation: AAK38378.1.
AC004511 Genomic DNA. Translation: AAC08706.1.
BC066272 mRNA. Translation: AAH66272.1.
BC066273 mRNA. Translation: AAH66273.1.
BC066276 mRNA. Translation: AAH66276.1.
BC069472 mRNA. Translation: AAH69472.1.

IPI

IPI00030336.

PIR

A24427.

RefSeq

NP_000579.2. NM_000588.3.

UniGene

Hs.694.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JLI	NMR	-	A	34-144	[»]

ProteinModelPortal

P08700.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6N.

IntAct

P08700. 1 interaction.

STRING

9606.ENSP00000296870.

PTM databases

PhosphoSite

P08700.

Polymorphism databases

DMDM

124330.

Proteomic databases

PaxDb

P08700.

PRIDE

P08700.

Protocols and materials databases

DNASU

3562.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000296870; ENSP00000296870; ENSG00000164399.

GeneID

3562.

KEGG

hsa:3562.

UCSC

uc003kwe.1. human.

Organism-specific databases

CTD

3562.

GeneCards

GC05P131396.

HGNC

HGNC:6011. IL3.

MIM

147740. gene.

neXtProt

NX_P08700.

PharmGKB

PA29830.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG72146.

HOGENOM

HOG000037939.

HOVERGEN

HBG004103.

InParanoid

P08700.

KO

K04736.

OMA

RPNLEAF.

OrthoDB

EOG4QNMXW.

PhylomeDB

P08700.

Enzyme and pathway databases

Pathway_Interaction_DB

nfat_tfpathway. Calcineurin-regulated NFAT-dependent transcription in lymphocytes.
tcrcalciumpathway. Calcium signaling in the CD4+ TCR pathway.

Reactome

REACT_6900. Immune System.

SignaLink

P08700.

Gene expression databases

Bgee

P08700.

CleanEx

HS_IL3.

Genevestigator

P08700.

GermOnline

ENSG00000164399. Homo sapiens.

Family and domain databases

Gene3D

1.20.1250.10. 1 hit.

InterPro

IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. Interleukin_3.
[Graphical view]

PANTHER

PTHR10079. PTHR10079. 1 hit.

Pfam

PF02059. IL3. 1 hit.
[Graphical view]

PIRSF

PIRSF001939. IL-3. 1 hit.

PRINTS

PR00430. INTERLEUKIN3.

SUPFAM

SSF47266. 4_helix_cytokine. 1 hit.

ProtoNet

Search...

Other

DrugBank

DB01025. Amlexanox.

EvolutionaryTrace

P08700.

GenomeRNAi

3562.

NextBio

13912.

SOURCE

Search...

Entry information

Entry name

IL3_HUMAN

Accession

Primary (citable) accession number: P08700
Secondary accession number(s): Q6GS87

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	October 1, 1989
Last modified:	May 29, 2013
This is version 140 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.