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P08700

- IL3_HUMAN

UniProt

P08700 - IL3_HUMAN

Protein

Interleukin-3

Gene

IL3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
    This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

    GO - Molecular functioni

    1. interleukin-3 receptor binding Source: UniProtKB

    GO - Biological processi

    1. cell-cell signaling Source: ProtInc
    2. embryonic hemopoiesis Source: DFLAT
    3. immune response Source: InterPro
    4. nervous system development Source: ProtInc
    5. positive regulation of cell proliferation Source: UniProtKB
    6. positive regulation of DNA replication Source: BHF-UCL
    7. positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
    8. positive regulation of tyrosine phosphorylation of Stat5 protein Source: BHF-UCL

    Keywords - Molecular functioni

    Cytokine, Growth factor

    Enzyme and pathway databases

    ReactomeiREACT_23837. Interleukin-3, 5 and GM-CSF signaling.
    REACT_23891. Interleukin receptor SHC signaling.
    SignaLinkiP08700.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin-3
    Short name:
    IL-3
    Alternative name(s):
    Hematopoietic growth factor
    Mast cell growth factor
    Short name:
    MCGF
    Multipotential colony-stimulating factor
    P-cell-stimulating factor
    Gene namesi
    Name:IL3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:6011. IL3.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29830.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Add
    BLAST
    Chaini20 – 152133Interleukin-3PRO_0000015516Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi35 ↔ 103
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP08700.
    PRIDEiP08700.

    PTM databases

    PhosphoSiteiP08700.

    Expressioni

    Tissue specificityi

    Activated T-cells, mast cells, natural killer cells.

    Gene expression databases

    BgeeiP08700.
    CleanExiHS_IL3.
    GenevestigatoriP08700.

    Interactioni

    Subunit structurei

    Monomer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CSF2RBP329271EBI-1811718,EBI-1809771

    Protein-protein interaction databases

    BioGridi109777. 3 interactions.
    DIPiDIP-6N.
    IntActiP08700. 1 interaction.
    STRINGi9606.ENSP00000296870.

    Structurei

    Secondary structure

    1
    152
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi35 – 4511
    Helixi61 – 688
    Helixi73 – 8614
    Helixi91 – 10010
    Helixi101 – 1033
    Helixi123 – 14119

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JLINMR-A34-144[»]
    ProteinModelPortaliP08700.
    SMRiP08700. Positions 34-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08700.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the IL-3 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG72146.
    HOGENOMiHOG000037939.
    HOVERGENiHBG004103.
    InParanoidiP08700.
    KOiK04736.
    OMAiQNASAIE.
    OrthoDBiEOG7NKKNN.
    PhylomeDBiP08700.
    TreeFamiTF338567.

    Family and domain databases

    Gene3Di1.20.1250.10. 1 hit.
    InterProiIPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR002183. IL-3.
    [Graphical view]
    PANTHERiPTHR10079. PTHR10079. 1 hit.
    PfamiPF02059. IL3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001939. IL-3. 1 hit.
    PRINTSiPR00430. INTERLEUKIN3.
    SUPFAMiSSF47266. SSF47266. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08700-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRLPVLLLL QLLVRPGLQA PMTQTTPLKT SWVNCSNMID EIITHLKQPP    50
    LPLLDFNNLN GEDQDILMEN NLRRPNLEAF NRAVKSLQNA SAIESILKNL 100
    LPCLPLATAA PTRHPIHIKD GDWNEFRRKL TFYLKTLENA QAQQTTLSLA 150
    IF 152
    Length:152
    Mass (Da):17,233
    Last modified:October 1, 1989 - v2
    Checksum:iBB46F23FAC1259A4
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti3 – 31R → C.
    Corresponds to variant rs35415145 [ dbSNP | Ensembl ].
    VAR_034014
    Natural varianti15 – 151R → H.1 Publication
    Corresponds to variant rs2069787 [ dbSNP | Ensembl ].
    VAR_013071
    Natural varianti27 – 271P → S.3 Publications
    Corresponds to variant rs40401 [ dbSNP | Ensembl ].
    VAR_013072
    Natural varianti60 – 601N → S.
    Corresponds to variant rs35482671 [ dbSNP | Ensembl ].
    VAR_034015

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14743 mRNA. Translation: AAA59146.1.
    M20137 mRNA. Translation: AAA59147.1.
    M17115 mRNA. Translation: AAA35725.1.
    AF365976 Genomic DNA. Translation: AAK38378.1.
    AC004511 Genomic DNA. Translation: AAC08706.1.
    BC066272 mRNA. Translation: AAH66272.1.
    BC066273 mRNA. Translation: AAH66273.1.
    BC066276 mRNA. Translation: AAH66276.1.
    BC069472 mRNA. Translation: AAH69472.1.
    CCDSiCCDS4149.1.
    PIRiA24427.
    RefSeqiNP_000579.2. NM_000588.3.
    UniGeneiHs.694.

    Genome annotation databases

    EnsembliENST00000296870; ENSP00000296870; ENSG00000164399.
    GeneIDi3562.
    KEGGihsa:3562.
    UCSCiuc003kwe.1. human.

    Polymorphism databases

    DMDMi124330.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology
    SeattleSNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14743 mRNA. Translation: AAA59146.1 .
    M20137 mRNA. Translation: AAA59147.1 .
    M17115 mRNA. Translation: AAA35725.1 .
    AF365976 Genomic DNA. Translation: AAK38378.1 .
    AC004511 Genomic DNA. Translation: AAC08706.1 .
    BC066272 mRNA. Translation: AAH66272.1 .
    BC066273 mRNA. Translation: AAH66273.1 .
    BC066276 mRNA. Translation: AAH66276.1 .
    BC069472 mRNA. Translation: AAH69472.1 .
    CCDSi CCDS4149.1.
    PIRi A24427.
    RefSeqi NP_000579.2. NM_000588.3.
    UniGenei Hs.694.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JLI NMR - A 34-144 [» ]
    ProteinModelPortali P08700.
    SMRi P08700. Positions 34-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109777. 3 interactions.
    DIPi DIP-6N.
    IntActi P08700. 1 interaction.
    STRINGi 9606.ENSP00000296870.

    Chemistry

    DrugBanki DB01025. Amlexanox.

    PTM databases

    PhosphoSitei P08700.

    Polymorphism databases

    DMDMi 124330.

    Proteomic databases

    PaxDbi P08700.
    PRIDEi P08700.

    Protocols and materials databases

    DNASUi 3562.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000296870 ; ENSP00000296870 ; ENSG00000164399 .
    GeneIDi 3562.
    KEGGi hsa:3562.
    UCSCi uc003kwe.1. human.

    Organism-specific databases

    CTDi 3562.
    GeneCardsi GC05P131396.
    HGNCi HGNC:6011. IL3.
    MIMi 147740. gene.
    neXtProti NX_P08700.
    PharmGKBi PA29830.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG72146.
    HOGENOMi HOG000037939.
    HOVERGENi HBG004103.
    InParanoidi P08700.
    KOi K04736.
    OMAi QNASAIE.
    OrthoDBi EOG7NKKNN.
    PhylomeDBi P08700.
    TreeFami TF338567.

    Enzyme and pathway databases

    Reactomei REACT_23837. Interleukin-3, 5 and GM-CSF signaling.
    REACT_23891. Interleukin receptor SHC signaling.
    SignaLinki P08700.

    Miscellaneous databases

    EvolutionaryTracei P08700.
    GeneWikii Interleukin_3.
    GenomeRNAii 3562.
    NextBioi 13912.
    PROi P08700.
    SOURCEi Search...

    Gene expression databases

    Bgeei P08700.
    CleanExi HS_IL3.
    Genevestigatori P08700.

    Family and domain databases

    Gene3Di 1.20.1250.10. 1 hit.
    InterProi IPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR002183. IL-3.
    [Graphical view ]
    PANTHERi PTHR10079. PTHR10079. 1 hit.
    Pfami PF02059. IL3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001939. IL-3. 1 hit.
    PRINTSi PR00430. INTERLEUKIN3.
    SUPFAMi SSF47266. SSF47266. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a human multilineage-colony-stimulating factor cDNA clone identified by a conserved noncoding sequence in mouse interleukin-3."
      Dorssers L., Burger H., Bot F., Delwel R., Geurts van Kessel A.H.M., Loewenberg B., Wagemaker G.
      Gene 55:115-124(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation and characterization of an expressible cDNA encoding human IL-3. Induction of IL-3 mRNA in human T cell clones."
      Otsuka T., Miyajima A., Brown N., Otsu K., Abrams J., Saeland S., Caux C., de Waal Malefijt R., de Vries J., Meyerson P., Yokota K., Gemmel L., Rennick D., Lee F., Arai N., Arai K., Yokota T.
      J. Immunol. 140:2288-2295(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3."
      Yang Y.-C., Ciarletta A.B., Temple P.A., Chung M.P., Kovacic S., Witek-Giannotti J.S., Leary A.C., Kriz R., Donahue R.E., Wong G.G., Clark S.C.
      Cell 47:3-10(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. SeattleSNPs variation discovery resource
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-15 AND SER-27.
    5. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-27.
    7. "Molecular characterization of colony-stimulating factors and their receptors: human interleukin-3."
      Urdal D.L., Price V., Sassenfeld H.M., Cosman D., Gillis S., Park L.S.
      Ann. N. Y. Acad. Sci. 554:167-176(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 20-152, VARIANT SER-27.
    8. "Three-dimensional solution structure and backbone dynamics of a variant of human interleukin-3."
      Feng Y., Klein B.K., McWherter C.A.
      J. Mol. Biol. 259:524-541(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiIL3_HUMAN
    AccessioniPrimary (citable) accession number: P08700
    Secondary accession number(s): Q6GS87
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 152 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3