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P08700 (IL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 150. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-3

Short name=IL-3
Alternative name(s):
Hematopoietic growth factor
Mast cell growth factor
Short name=MCGF
Multipotential colony-stimulating factor
P-cell-stimulating factor
Gene names
Name:IL3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Subunit structure

Monomer.

Subcellular location

Secreted.

Tissue specificity

Activated T-cells, mast cells, natural killer cells.

Sequence similarities

Belongs to the IL-3 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CSF2RBP329271EBI-1811718,EBI-1809771

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919
Chain20 – 152133Interleukin-3
PRO_0000015516

Amino acid modifications

Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation891N-linked (GlcNAc...) Potential
Disulfide bond35 ↔ 103

Natural variations

Natural variant31R → C.
Corresponds to variant rs35415145 [ dbSNP | Ensembl ].
VAR_034014
Natural variant151R → H. Ref.4
Corresponds to variant rs2069787 [ dbSNP | Ensembl ].
VAR_013071
Natural variant271P → S. Ref.4 Ref.6 Ref.7
Corresponds to variant rs40401 [ dbSNP | Ensembl ].
VAR_013072
Natural variant601N → S.
Corresponds to variant rs35482671 [ dbSNP | Ensembl ].
VAR_034015

Secondary structure

............ 152
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08700 [UniParc].

Last modified October 1, 1989. Version 2.
Checksum: BB46F23FAC1259A4

FASTA15217,233
        10         20         30         40         50         60 
MSRLPVLLLL QLLVRPGLQA PMTQTTPLKT SWVNCSNMID EIITHLKQPP LPLLDFNNLN 

        70         80         90        100        110        120 
GEDQDILMEN NLRRPNLEAF NRAVKSLQNA SAIESILKNL LPCLPLATAA PTRHPIHIKD 

       130        140        150 
GDWNEFRRKL TFYLKTLENA QAQQTTLSLA IF 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a human multilineage-colony-stimulating factor cDNA clone identified by a conserved noncoding sequence in mouse interleukin-3."
Dorssers L., Burger H., Bot F., Delwel R., Geurts van Kessel A.H.M., Loewenberg B., Wagemaker G.
Gene 55:115-124(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation and characterization of an expressible cDNA encoding human IL-3. Induction of IL-3 mRNA in human T cell clones."
Otsuka T., Miyajima A., Brown N., Otsu K., Abrams J., Saeland S., Caux C., de Waal Malefijt R., de Vries J., Meyerson P., Yokota K., Gemmel L., Rennick D., Lee F., Arai N., Arai K., Yokota T.
J. Immunol. 140:2288-2295(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3."
Yang Y.-C., Ciarletta A.B., Temple P.A., Chung M.P., Kovacic S., Witek-Giannotti J.S., Leary A.C., Kriz R., Donahue R.E., Wong G.G., Clark S.C.
Cell 47:3-10(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]SeattleSNPs variation discovery resource
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-15 AND SER-27.
[5]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-27.
[7]"Molecular characterization of colony-stimulating factors and their receptors: human interleukin-3."
Urdal D.L., Price V., Sassenfeld H.M., Cosman D., Gillis S., Park L.S.
Ann. N. Y. Acad. Sci. 554:167-176(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 20-152, VARIANT SER-27.
[8]"Three-dimensional solution structure and backbone dynamics of a variant of human interleukin-3."
Feng Y., Klein B.K., McWherter C.A.
J. Mol. Biol. 259:524-541(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M14743 mRNA. Translation: AAA59146.1.
M20137 mRNA. Translation: AAA59147.1.
M17115 mRNA. Translation: AAA35725.1.
AF365976 Genomic DNA. Translation: AAK38378.1.
AC004511 Genomic DNA. Translation: AAC08706.1.
BC066272 mRNA. Translation: AAH66272.1.
BC066273 mRNA. Translation: AAH66273.1.
BC066276 mRNA. Translation: AAH66276.1.
BC069472 mRNA. Translation: AAH69472.1.
CCDSCCDS4149.1.
PIRA24427.
RefSeqNP_000579.2. NM_000588.3.
UniGeneHs.694.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JLINMR-A34-144[»]
ProteinModelPortalP08700.
SMRP08700. Positions 34-144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109777. 3 interactions.
DIPDIP-6N.
IntActP08700. 1 interaction.
STRING9606.ENSP00000296870.

Chemistry

DrugBankDB01025. Amlexanox.

PTM databases

PhosphoSiteP08700.

Polymorphism databases

DMDM124330.

Proteomic databases

PaxDbP08700.
PRIDEP08700.

Protocols and materials databases

DNASU3562.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000296870; ENSP00000296870; ENSG00000164399.
GeneID3562.
KEGGhsa:3562.
UCSCuc003kwe.1. human.

Organism-specific databases

CTD3562.
GeneCardsGC05P131396.
HGNCHGNC:6011. IL3.
MIM147740. gene.
neXtProtNX_P08700.
PharmGKBPA29830.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG72146.
HOGENOMHOG000037939.
HOVERGENHBG004103.
InParanoidP08700.
KOK04736.
OMAQNASAIE.
OrthoDBEOG7NKKNN.
PhylomeDBP08700.
TreeFamTF338567.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
SignaLinkP08700.

Gene expression databases

BgeeP08700.
CleanExHS_IL3.
GenevestigatorP08700.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. IL-3.
[Graphical view]
PANTHERPTHR10079. PTHR10079. 1 hit.
PfamPF02059. IL3. 1 hit.
[Graphical view]
PIRSFPIRSF001939. IL-3. 1 hit.
PRINTSPR00430. INTERLEUKIN3.
SUPFAMSSF47266. SSF47266. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP08700.
GeneWikiInterleukin_3.
GenomeRNAi3562.
NextBio13912.
PROP08700.
SOURCESearch...

Entry information

Entry nameIL3_HUMAN
AccessionPrimary (citable) accession number: P08700
Secondary accession number(s): Q6GS87
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 1, 1989
Last modified: July 9, 2014
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM