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Protein

Interleukin-3

Gene

IL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

GO - Molecular functioni

  1. cytokine activity Source: GO_Central
  2. interleukin-3 receptor binding Source: UniProtKB

GO - Biological processi

  1. cell-cell signaling Source: ProtInc
  2. cytokine-mediated signaling pathway Source: GO_Central
  3. embryonic hemopoiesis Source: DFLAT
  4. immune response Source: InterPro
  5. nervous system development Source: ProtInc
  6. positive regulation of cell proliferation Source: UniProtKB
  7. positive regulation of DNA replication Source: BHF-UCL
  8. positive regulation of mast cell proliferation Source: GO_Central
  9. positive regulation of myeloid leukocyte differentiation Source: GO_Central
  10. positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
  11. positive regulation of tyrosine phosphorylation of Stat5 protein Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Enzyme and pathway databases

ReactomeiREACT_1695. GPVI-mediated activation cascade.
REACT_19290. G beta:gamma signalling through PI3Kgamma.
REACT_23837. Interleukin-3, 5 and GM-CSF signaling.
REACT_23891. Interleukin receptor SHC signaling.
SignaLinkiP08700.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-3
Short name:
IL-3
Alternative name(s):
Hematopoietic growth factor
Mast cell growth factor
Short name:
MCGF
Multipotential colony-stimulating factor
P-cell-stimulating factor
Gene namesi
Name:IL3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:6011. IL3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29830.

Chemistry

DrugBankiDB01025. Amlexanox.

Polymorphism and mutation databases

BioMutaiIL3.
DMDMi124330.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 152133Interleukin-3PRO_0000015516Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi35 ↔ 103
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP08700.
PRIDEiP08700.

PTM databases

PhosphoSiteiP08700.

Expressioni

Tissue specificityi

Activated T-cells, mast cells, natural killer cells.

Gene expression databases

BgeeiP08700.
CleanExiHS_IL3.
GenevestigatoriP08700.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
CSF2RBP329271EBI-1811718,EBI-1809771

Protein-protein interaction databases

BioGridi109777. 3 interactions.
DIPiDIP-6N.
IntActiP08700. 1 interaction.
STRINGi9606.ENSP00000296870.

Structurei

Secondary structure

1
152
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 4511Combined sources
Helixi61 – 688Combined sources
Helixi73 – 8614Combined sources
Helixi91 – 10010Combined sources
Helixi101 – 1033Combined sources
Helixi123 – 14119Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JLINMR-A34-144[»]
ProteinModelPortaliP08700.
SMRiP08700. Positions 34-144.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08700.

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-3 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG72146.
GeneTreeiENSGT00730000111772.
HOGENOMiHOG000037939.
HOVERGENiHBG004103.
InParanoidiP08700.
KOiK04736.
OMAiQNASAIE.
OrthoDBiEOG7NKKNN.
PhylomeDBiP08700.
TreeFamiTF338567.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. IL-3.
[Graphical view]
PANTHERiPTHR10079. PTHR10079. 1 hit.
PfamiPF02059. IL3. 1 hit.
[Graphical view]
PIRSFiPIRSF001939. IL-3. 1 hit.
PRINTSiPR00430. INTERLEUKIN3.
SUPFAMiSSF47266. SSF47266. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLPVLLLL QLLVRPGLQA PMTQTTPLKT SWVNCSNMID EIITHLKQPP
60 70 80 90 100
LPLLDFNNLN GEDQDILMEN NLRRPNLEAF NRAVKSLQNA SAIESILKNL
110 120 130 140 150
LPCLPLATAA PTRHPIHIKD GDWNEFRRKL TFYLKTLENA QAQQTTLSLA

IF
Length:152
Mass (Da):17,233
Last modified:October 1, 1989 - v2
Checksum:iBB46F23FAC1259A4
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3 – 31R → C.
Corresponds to variant rs35415145 [ dbSNP | Ensembl ].
VAR_034014
Natural varianti15 – 151R → H.1 Publication
Corresponds to variant rs2069787 [ dbSNP | Ensembl ].
VAR_013071
Natural varianti27 – 271P → S.3 Publications
Corresponds to variant rs40401 [ dbSNP | Ensembl ].
VAR_013072
Natural varianti60 – 601N → S.
Corresponds to variant rs35482671 [ dbSNP | Ensembl ].
VAR_034015

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14743 mRNA. Translation: AAA59146.1.
M20137 mRNA. Translation: AAA59147.1.
M17115 mRNA. Translation: AAA35725.1.
AF365976 Genomic DNA. Translation: AAK38378.1.
AC004511 Genomic DNA. Translation: AAC08706.1.
BC066272 mRNA. Translation: AAH66272.1.
BC066273 mRNA. Translation: AAH66273.1.
BC066276 mRNA. Translation: AAH66276.1.
BC069472 mRNA. Translation: AAH69472.1.
CCDSiCCDS4149.1.
PIRiA24427.
RefSeqiNP_000579.2. NM_000588.3.
UniGeneiHs.694.

Genome annotation databases

EnsembliENST00000296870; ENSP00000296870; ENSG00000164399.
GeneIDi3562.
KEGGihsa:3562.
UCSCiuc003kwe.1. human.

Polymorphism and mutation databases

BioMutaiIL3.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14743 mRNA. Translation: AAA59146.1.
M20137 mRNA. Translation: AAA59147.1.
M17115 mRNA. Translation: AAA35725.1.
AF365976 Genomic DNA. Translation: AAK38378.1.
AC004511 Genomic DNA. Translation: AAC08706.1.
BC066272 mRNA. Translation: AAH66272.1.
BC066273 mRNA. Translation: AAH66273.1.
BC066276 mRNA. Translation: AAH66276.1.
BC069472 mRNA. Translation: AAH69472.1.
CCDSiCCDS4149.1.
PIRiA24427.
RefSeqiNP_000579.2. NM_000588.3.
UniGeneiHs.694.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JLINMR-A34-144[»]
ProteinModelPortaliP08700.
SMRiP08700. Positions 34-144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109777. 3 interactions.
DIPiDIP-6N.
IntActiP08700. 1 interaction.
STRINGi9606.ENSP00000296870.

Chemistry

DrugBankiDB01025. Amlexanox.

PTM databases

PhosphoSiteiP08700.

Polymorphism and mutation databases

BioMutaiIL3.
DMDMi124330.

Proteomic databases

PaxDbiP08700.
PRIDEiP08700.

Protocols and materials databases

DNASUi3562.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296870; ENSP00000296870; ENSG00000164399.
GeneIDi3562.
KEGGihsa:3562.
UCSCiuc003kwe.1. human.

Organism-specific databases

CTDi3562.
GeneCardsiGC05P131396.
HGNCiHGNC:6011. IL3.
MIMi147740. gene.
neXtProtiNX_P08700.
PharmGKBiPA29830.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG72146.
GeneTreeiENSGT00730000111772.
HOGENOMiHOG000037939.
HOVERGENiHBG004103.
InParanoidiP08700.
KOiK04736.
OMAiQNASAIE.
OrthoDBiEOG7NKKNN.
PhylomeDBiP08700.
TreeFamiTF338567.

Enzyme and pathway databases

ReactomeiREACT_1695. GPVI-mediated activation cascade.
REACT_19290. G beta:gamma signalling through PI3Kgamma.
REACT_23837. Interleukin-3, 5 and GM-CSF signaling.
REACT_23891. Interleukin receptor SHC signaling.
SignaLinkiP08700.

Miscellaneous databases

EvolutionaryTraceiP08700.
GeneWikiiInterleukin_3.
GenomeRNAii3562.
NextBioi13912.
PROiP08700.
SOURCEiSearch...

Gene expression databases

BgeeiP08700.
CleanExiHS_IL3.
GenevestigatoriP08700.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR002183. IL-3.
[Graphical view]
PANTHERiPTHR10079. PTHR10079. 1 hit.
PfamiPF02059. IL3. 1 hit.
[Graphical view]
PIRSFiPIRSF001939. IL-3. 1 hit.
PRINTSiPR00430. INTERLEUKIN3.
SUPFAMiSSF47266. SSF47266. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a human multilineage-colony-stimulating factor cDNA clone identified by a conserved noncoding sequence in mouse interleukin-3."
    Dorssers L., Burger H., Bot F., Delwel R., Geurts van Kessel A.H.M., Loewenberg B., Wagemaker G.
    Gene 55:115-124(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of an expressible cDNA encoding human IL-3. Induction of IL-3 mRNA in human T cell clones."
    Otsuka T., Miyajima A., Brown N., Otsu K., Abrams J., Saeland S., Caux C., de Waal Malefijt R., de Vries J., Meyerson P., Yokota K., Gemmel L., Rennick D., Lee F., Arai N., Arai K., Yokota T.
    J. Immunol. 140:2288-2295(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3."
    Yang Y.-C., Ciarletta A.B., Temple P.A., Chung M.P., Kovacic S., Witek-Giannotti J.S., Leary A.C., Kriz R., Donahue R.E., Wong G.G., Clark S.C.
    Cell 47:3-10(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. SeattleSNPs variation discovery resource
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-15 AND SER-27.
  5. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-27.
  7. "Molecular characterization of colony-stimulating factors and their receptors: human interleukin-3."
    Urdal D.L., Price V., Sassenfeld H.M., Cosman D., Gillis S., Park L.S.
    Ann. N. Y. Acad. Sci. 554:167-176(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 20-152, VARIANT SER-27.
  8. "Three-dimensional solution structure and backbone dynamics of a variant of human interleukin-3."
    Feng Y., Klein B.K., McWherter C.A.
    J. Mol. Biol. 259:524-541(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiIL3_HUMAN
AccessioniPrimary (citable) accession number: P08700
Secondary accession number(s): Q6GS87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 1, 1989
Last modified: April 29, 2015
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.