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Protein

Galectin-3

Gene

Lgals3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells (By similarity). Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.By similarity1 Publication

GO - Molecular functioni

  • advanced glycation end-product receptor activity Source: RGD
  • disaccharide binding Source: RGD
  • Fc-gamma receptor I complex binding Source: RGD
  • IgE binding Source: RGD
  • monosaccharide binding Source: RGD

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • mRNA processing Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of cell proliferation in bone marrow Source: RGD
  • positive regulation of angiogenesis Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of serotonin secretion Source: RGD
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Differentiation, Immunity, Innate immunity, mRNA processing, mRNA splicing

Keywords - Ligandi

IgE-binding protein, Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Galectin-3
Short name:
Gal-3
Alternative name(s):
35 kDa lectin
Carbohydrate-binding protein 35
Short name:
CBP 35
Galactose-specific lectin 3
IgE-binding protein
Laminin-binding protein
Lectin L-29
Mac-2 antigen
Gene namesi
Name:Lgals3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi69356. Lgals3.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Secreted By similarity

  • Note: Secreted by a non-classical secretory pathway and associates with the cell surface.By similarity

GO - Cellular componenti

  • cell surface Source: RGD
  • cytoplasm Source: RGD
  • extracellular space Source: RGD
  • nucleus Source: RGD
  • plasma membrane Source: Reactome
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 262261Galectin-3PRO_0000076933Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei6 – 61Phosphoserine; by CK1By similarity
Disulfide bondi185 – 185InterchainBy similarity
Modified residuei200 – 2001PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP08699.
PRIDEiP08699.

PTM databases

iPTMnetiP08699.
PhosphoSiteiP08699.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010645.
ExpressionAtlasiP08699. baseline and differential.
GenevisibleiP08699. RN.

Interactioni

Subunit structurei

Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Interacts with CD6 and ALCAM. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA (By similarity).By similarity2 Publications

GO - Molecular functioni

  • Fc-gamma receptor I complex binding Source: RGD
  • IgE binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014216.

Structurei

3D structure databases

ProteinModelPortaliP08699.
SMRiP08699. Positions 126-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati35 – 4391
Repeati44 – 5292
Repeati53 – 6193
Repeati62 – 7094
Repeati71 – 7995
Repeati80 – 8896
Repeati89 – 98107; approximate
Repeati99 – 10578; approximate
Repeati106 – 11279; truncated
Domaini130 – 260131GalectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 112789 X 9 AA tandem repeats of Y-P-G-X(3)-P-[GS]-[AG]Add
BLAST
Regioni193 – 1997Beta-galactoside bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi238 – 25316Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 galectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
HOGENOMiHOG000246423.
HOVERGENiHBG006255.
InParanoidiP08699.
KOiK06831.
PhylomeDBiP08699.
TreeFamiTF315551.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
[Graphical view]
PfamiPF00337. Gal-bind_lectin. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGFSLNDA LAGSGNPNPQ GWPGAWGNQP GAGGYPGASY PGAYPGQAPP
60 70 80 90 100
GGYPGQAPPS AYPGPTGPSA YPGPTAPGAY PGPTAPGAFP GQPGGPGAYP
110 120 130 140 150
SAPGAYPSAP GAYPATGPFG APTGPLTVPY DMPLPGGVMP RMLITIIGTV
160 170 180 190 200
KPNANSITLN FKKGNDIAFH FNPRFNENNR RVIVCNTKQD NNWGREERQS
210 220 230 240 250
AFPFESGKPF KIQVLVEADH FKVAVNDVHL LQYNHRMKNL REISQLGIIG
260
DITLTSASHA MI
Length:262
Mass (Da):27,202
Last modified:January 23, 2007 - v4
Checksum:iEADB994F5EBD493D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201Q → R in AAA40828 (PubMed:2958848).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02962 mRNA. Translation: AAA40828.1.
BC089054 mRNA. Translation: AAH89054.1.
M13697 mRNA. Translation: AAA41378.1.
PIRiA54889.
RefSeqiNP_114020.1. NM_031832.1.
UniGeneiRn.764.

Genome annotation databases

EnsembliENSRNOT00000014216; ENSRNOP00000014216; ENSRNOG00000010645.
GeneIDi83781.
KEGGirno:83781.
UCSCiRGD:69356. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02962 mRNA. Translation: AAA40828.1.
BC089054 mRNA. Translation: AAH89054.1.
M13697 mRNA. Translation: AAA41378.1.
PIRiA54889.
RefSeqiNP_114020.1. NM_031832.1.
UniGeneiRn.764.

3D structure databases

ProteinModelPortaliP08699.
SMRiP08699. Positions 126-262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014216.

PTM databases

iPTMnetiP08699.
PhosphoSiteiP08699.

Proteomic databases

PaxDbiP08699.
PRIDEiP08699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014216; ENSRNOP00000014216; ENSRNOG00000010645.
GeneIDi83781.
KEGGirno:83781.
UCSCiRGD:69356. rat.

Organism-specific databases

CTDi3958.
RGDi69356. Lgals3.

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
HOGENOMiHOG000246423.
HOVERGENiHBG006255.
InParanoidiP08699.
KOiK06831.
PhylomeDBiP08699.
TreeFamiTF315551.

Miscellaneous databases

PROiP08699.

Gene expression databases

BgeeiENSRNOG00000010645.
ExpressionAtlasiP08699. baseline and differential.
GenevisibleiP08699. RN.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001079. Galectin_CRD.
[Graphical view]
PfamiPF00337. Gal-bind_lectin. 1 hit.
[Graphical view]
SMARTiSM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51304. GALECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEG3_RAT
AccessioniPrimary (citable) accession number: P08699
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 148 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.