Reviewed, UniProtKB/Swiss-Prot P08695 (BPHC_PSEPS)

Names and origin

Protein names

Recommended name:
    Biphenyl-2,3-diol 1,2-dioxygenase
    EC=1.13.11.39
Alternative name(s):
    23OHBP oxygenase
    2,3-dihydroxybiphenyl dioxygenase
      Short name=DHBD

Gene names

Name:

bphC

Organism

Pseudomonas pseudoalcaligenes

Taxonomic identifier

330 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Biphenyl-2,3-diol + O2 = 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O.
Cofactor	Fe2+ ion.
Pathway	Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl: step 3/4.
Subunit structure	Homooctamer.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	biphenyl-2,3-diol 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 303	302	Biphenyl-2,3-diol 1,2-dioxygenase		PRO_0000085034
Sites
	Metal binding	146	1	Iron By similarity
	Metal binding	210	1	Iron By similarity
	Metal binding	260	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08695-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6844D0ABBD81FED5	FASTA	303	33,206
	10 20 30 40 50 60 MSIRSLGYMG FAVSDVAAWR SFLTQKLGLM EAGTTDNGDL FRIDSRAWRI AVQQGEVDDL 70 80 90 100 110 120 AFAGYEVADA AGLAQMADKL KQAGIAVTTG DASLARRRGV TGLITFADPF GLPLEIYYGA 130 140 150 160 170 180 SEVFEKPFLP GAAVSGFLTG EQGLGHFVRC VPDSDKALAF YTDVLGFQLS DVIDMKMGPD 190 200 210 220 230 240 VTVPVYFLHC NERHHTLAIA AFPLPKRIHH FMLEVASLDD VGFAFDRVDA DGLITSTLGR 250 260 270 280 290 300 HTNDHMVSFY ASTPSGVEVE YGWSARTVDR SWVVVRHDSP SMWGHKSVRD KALRATKHEQ QPE

References

[1]	"Nucleotide sequence of the 2,3-dihydroxybiphenyl dioxygenase gene of Pseudomonas pseudoalcaligenes." Furukawa K., Arimura N., Miyazaki T. J. Bacteriol. 169:427-429(1987) [PubMed: 3793719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KF707.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15333 Genomic DNA. Translation: AAA25753.1.
3D structure databases
SMR	P08695. Positions 2-289.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.39. 3804.
Family and domain databases
InterPro	IPR017626. DiOHbiphenyl_dOase. IPR004360. Glyas_bleo-R_dOase. IPR000486. Xdiol_ring_cleave_dOase_1/2. [Graphical view]
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03213. 23dbph12diox. 1 hit.
PROSITE	PS00082. EXTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BPHC_PSEPS

Accession

Primary (citable) accession number: P08695

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)