Reviewed, UniProtKB/Swiss-Prot P08694 (BPHB_PSEPS)

Names and origin

Protein names

Recommended name:
    Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
    EC=1.3.1.56
Alternative name(s):
    Biphenyl-2,3-dihydro-2,3-diol dehydrogenase
    Biphenyl-cis-diol dehydrogenase
    2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase
    2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase

Gene names

Name:

bphB

Organism

Pseudomonas pseudoalcaligenes

Taxonomic identifier

330 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cis-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+ = biphenyl-2,3-diol + NADH.
Pathway	Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoic acid and benzoic acid from biphenyl: step 2/4.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase		PRO_0000054533
Regions
	Nucleotide binding	9 – 33	25	NAD By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	140	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08694-1 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: F32A4A870B47E566	FASTA	275	28,726
	10 20 30 40 50 60 MKLKGEAVLI TGGASGLGRA LVDRFVAEAK VAVLDKSAER LAELETDLGD NVLGIVGDVR 70 80 90 100 110 120 SLEDQKQAAS RCVARFGKID TLIPNAGIWD YSTALVDLPE ESLDAAFDEV FHINVKGYIH 130 140 150 160 170 180 AVKALPALVA SRGNVIFTIS NAGFYPNGGG PLYTAAKQAI VGLVRELAFE LAPYVRVNGV 190 200 210 220 230 240 GPGGMNSDMR GPSSLGMGSK AISTVPLADM LKSVLPIGRM PEVEEYTGAY VFFATRGDAA 250 260 270 PASGALVNYD GGLGVRGFFS GAGGNDLLEQ LNIHP

References

[1]	"Nucleotide sequence of the 2,3-dihydroxybiphenyl dioxygenase gene of Pseudomonas pseudoalcaligenes." Furukawa K., Arimura N., Miyazaki T. J. Bacteriol. 169:427-429(1987) [PubMed: 3793719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KF707.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15333 Genomic DNA. Translation: AAA25752.1. Different initiation.
3D structure databases
HSSP	HSSP built from PDB template 1BDB based on UniProtKB P47227.
SMR	P08694. Positions 1-274.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.56. 3804.
Family and domain databases
InterPro	IPR017711. BphB_TodD. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR03325. BphB_TodD. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BPHB_PSEPS

Accession

Primary (citable) accession number: P08694

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)