Reviewed, UniProtKB/Swiss-Prot P08692 (ARSC1_ECOLX)

Names and origin

Protein names

Recommended name:
    Arsenate reductase
    EC=1.20.4.1
Alternative name(s):
    Arsenical pump modifier

Gene names

Name:

arsC

Encoded on

Plasmid R773

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduction of arsenate [As(V)] to arsenite [As(III)]. This protein expands the substrate specificity of arsAB pump which can extrude arsenite and antimonite to allow for arsenate pumping and resistance.
Catalytic activity	Arsenate + glutaredoxin = arsenite + glutaredoxin disulfide + H2O.
Sequence similarities	Belongs to the arsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (glutaredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 141

141

Arsenate reductase

PRO_0000162537

Secondary structure

1

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141

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Helix

14 – 24

11

Beta strand

30 – 32

3

Turn

34 – 36

3

Helix

41 – 51

11

Helix

55 – 58

4

Helix

64 – 68

5

Turn

69 – 72

4

Helix

78 – 87

10

Helix

89 – 91

3

Beta strand

96 – 99

4

Beta strand

102 – 105

4

Helix

109 – 114

6

Beta strand

131 – 133

3

Sequences

	Sequence		Length	Mass (Da)
	P08692-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 240F4105487E2FBF	FASTA	141	15,830
	10 20 30 40 50 60 MSNITIYHNP ACGTSRNTLE MIRNSGTEPT IILYLENPPS RDELVKLIAD MGISVRALLR 70 80 90 100 110 120 KNVEPYEQLG LAEDKFTDDQ LIDFMLQHPI LINRPIVVTP LGTRLCRPSE VVLDILQDAQ 130 140 KGAFTKEDGE KVVDEAGKRL K

References

[1]	"Nucleotide sequence of the structural genes for an anion pump. The plasmid-encoded arsenical resistance operon." Chen C.-M., Misra T.K., Silver S., Rosen B.P. J. Biol. Chem. 261:15030-15038(1986) [PubMed: 3021763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular analysis of an anion pump: purification of the ArsC protein." Rosen B.P., Weigel U., Monticello R.A., Edwards B.P.F. Arch. Biochem. Biophys. 284:381-385(1991) [PubMed: 1703401] [Abstract] Cited for: PROTEIN SEQUENCE OF 5-13.
[3]	"The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase." Rosen B.P. Res. Microbiol. 141:336-341(1990) [PubMed: 1704144] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J02591 Genomic DNA. Translation: AAA21096.1.

PIR

C25937.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I9D	X-ray	1.65	A	1-141	[»]
1J9B	X-ray	1.26	A	1-141	[»]
1JZW	X-ray	1.76	A	1-140	[»]
1S3C	X-ray	1.25	A	1-141	[»]
1S3D	X-ray	1.54	A	3-140	[»]
1SD8	X-ray	1.59	A	1-141	[»]
1SD9	X-ray	1.65	A	1-141	[»]
1SJZ	X-ray	1.80	A	1-140	[»]
1SK0	X-ray	1.80	A	1-140	[»]
1SK1	X-ray	1.55	A	1-140	[»]
1SK2	X-ray	1.54	A	1-140	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.20.4.1. 246.

Family and domain databases

InterPro

IPR006659. Arsenate_reductase.
IPR006660. Arsenate_reductase-like.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF03960. ArsC. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00014. arsC. 1 hit.

PROSITE

PS51353. ARSC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ARSC1_ECOLX

Accession

Primary (citable) accession number: P08692

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)