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P08690

- ARSA1_ECOLX

UniProt

P08690 - ARSA1_ECOLX

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Protein

Arsenical pump-driving ATPase

Gene

arsA

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Anion-transporting ATPase. Catalyzes the extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents.

Catalytic activityi

ATP + H2O + arsenite(In) = ADP + phosphate + arsenite(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228ATPSequence Analysis
Nucleotide bindingi334 – 3418ATPSequence Analysis

GO - Molecular functioni

  1. arsenite-transmembrane transporting ATPase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. detoxification of arsenic-containing substance Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arsenical resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.4.1.1. the arsenite-antimonite (arsab) efflux family.

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenical pump-driving ATPase (EC:3.6.3.16)
Alternative name(s):
Arsenical resistance ATPase
Arsenite-translocating ATPase
Arsenite-transporting ATPase
Gene namesi
Name:arsA
Encoded oniPlasmid R7730 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 583583Arsenical pump-driving ATPasePRO_0000152251Add
BLAST

Proteomic databases

PRIDEiP08690.

Interactioni

Protein-protein interaction databases

DIPiDIP-16995N.

Structurei

Secondary structure

1
583
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 43Combined sources
Beta strandi9 – 146Combined sources
Helixi21 – 3414Combined sources
Beta strandi39 – 435Combined sources
Helixi50 – 534Combined sources
Beta strandi71 – 755Combined sources
Helixi78 – 9013Combined sources
Helixi91 – 933Combined sources
Turni94 – 963Combined sources
Helixi99 – 10810Combined sources
Helixi112 – 12817Combined sources
Helixi132 – 1354Combined sources
Beta strandi137 – 1437Combined sources
Helixi147 – 1548Combined sources
Helixi156 – 1594Combined sources
Beta strandi167 – 1693Combined sources
Helixi170 – 1723Combined sources
Helixi174 – 1785Combined sources
Helixi183 – 19412Combined sources
Turni196 – 1983Combined sources
Beta strandi199 – 2079Combined sources
Helixi209 – 22517Combined sources
Beta strandi230 – 2378Combined sources
Helixi240 – 2434Combined sources
Helixi247 – 26115Combined sources
Helixi265 – 2684Combined sources
Beta strandi272 – 2765Combined sources
Helixi285 – 2906Combined sources
Helixi315 – 3239Combined sources
Beta strandi328 – 3336Combined sources
Helixi340 – 35314Combined sources
Beta strandi358 – 3636Combined sources
Beta strandi380 – 3845Combined sources
Helixi387 – 40317Combined sources
Helixi408 – 41710Combined sources
Helixi421 – 43212Combined sources
Turni433 – 4353Combined sources
Helixi436 – 4405Combined sources
Beta strandi442 – 4476Combined sources
Helixi452 – 4609Combined sources
Helixi482 – 4865Combined sources
Turni488 – 4903Combined sources
Beta strandi491 – 4977Combined sources
Helixi501 – 51616Combined sources
Beta strandi523 – 5308Combined sources
Helixi538 – 55518Combined sources
Turni556 – 5583Combined sources
Beta strandi560 – 5667Combined sources
Helixi575 – 5828Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F48X-ray2.30A1-583[»]
1IHUX-ray2.15A1-583[»]
1II0X-ray2.40A/B1-583[»]
1II9X-ray2.60A/B1-583[»]
ProteinModelPortaliP08690.
SMRiP08690. Positions 1-583.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08690.

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.Curated

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR025723. Anion-transp_ATPase-like_dom.
IPR027541. Ars_ATPase.
IPR016300. ATPase_ArsA/GET3.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10803. PTHR10803. 1 hit.
PfamiPF02374. ArsA_ATPase. 3 hits.
[Graphical view]
PIRSFiPIRSF001327. Arsenical_pump-driving_ATPase. 1 hit.
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR04291. arsen_driv_ArsA. 1 hit.
TIGR00345. GET3_arsA_TRC40. 1 hit.

Sequencei

Sequence statusi: Complete.

P08690-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQFLQNIPPY LFFTGKGGVG KTSISCATAI RLAEQGKRVL LVSTDPASNV
60 70 80 90 100
GQVFSQTIGI TIQAIASVPG LSALEIDPQA AAQQYRARIV DPIKGVLPDD
110 120 130 140 150
VVSSINEQLS GACTTEIAAF DEFTGLLTDA SLLTRFDHII FDTAPTGHTI
160 170 180 190 200
RLLQLPGAWS SFIDSNPEGA SCLGPMAGLE KQREQYAYAV EALSDPKRTR
210 220 230 240 250
LVLVARLQKS TLQEVARTHL ELAAIGLKNQ YLVINGVLPK TEAANDTLAA
260 270 280 290 300
AIWEREQEAL ANLPADLAGL PTDTLFLQPV NMVGVSALSR LLSTQPVASP
310 320 330 340 350
SSDEYLQQRP DIPSLSALVD DIARNEHGLI MLMGKGGVGK TTMAAAIAVR
360 370 380 390 400
LADMGFDVHL TTSDPAAHLS MTLNGSLNNL QVSRIDPHEE TERYRQHVLE
410 420 430 440 450
TKGKELDEAG KRLLEEDLRS PCTEEIAVFQ AFSRVIREAG KRFVVMDTAP
460 470 480 490 500
TGHTLLLLDA TGAYHREIAK KMGEKGHFTT PMMLLQDPER TKVLLVTLPE
510 520 530 540 550
TTPVLEAANL QADLERAGIH PWGWIINNSL SIADTRSPLL RMRAQQELPQ
560 570 580
IESVKRQHAS RVALVPVLAS EPTGIDKLKQ LAG
Length:583
Mass (Da):63,188
Last modified:January 1, 1988 - v1
Checksum:i658C5AF65E5E75F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02591 Genomic DNA. Translation: AAA21094.1.
PIRiA25937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02591 Genomic DNA. Translation: AAA21094.1 .
PIRi A25937.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F48 X-ray 2.30 A 1-583 [» ]
1IHU X-ray 2.15 A 1-583 [» ]
1II0 X-ray 2.40 A/B 1-583 [» ]
1II9 X-ray 2.60 A/B 1-583 [» ]
ProteinModelPortali P08690.
SMRi P08690. Positions 1-583.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-16995N.

Protein family/group databases

TCDBi 3.A.4.1.1. the arsenite-antimonite (arsab) efflux family.

Proteomic databases

PRIDEi P08690.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P08690.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR025723. Anion-transp_ATPase-like_dom.
IPR027541. Ars_ATPase.
IPR016300. ATPase_ArsA/GET3.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10803. PTHR10803. 1 hit.
Pfami PF02374. ArsA_ATPase. 3 hits.
[Graphical view ]
PIRSFi PIRSF001327. Arsenical_pump-driving_ATPase. 1 hit.
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR04291. arsen_driv_ArsA. 1 hit.
TIGR00345. GET3_arsA_TRC40. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the structural genes for an anion pump. The plasmid-encoded arsenical resistance operon."
    Chen C.-M., Misra T.K., Silver S., Rosen B.P.
    J. Biol. Chem. 261:15030-15038(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase."
    Rosen B.P.
    Res. Microbiol. 141:336-341(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiARSA1_ECOLX
AccessioniPrimary (citable) accession number: P08690
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 26, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3