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P08690

- ARSA1_ECOLX

UniProt

P08690 - ARSA1_ECOLX

Protein

Arsenical pump-driving ATPase

Gene

arsA

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Anion-transporting ATPase. Catalyzes the extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents.

    Catalytic activityi

    ATP + H2O + arsenite(In) = ADP + phosphate + arsenite(Out).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228ATPSequence Analysis
    Nucleotide bindingi334 – 3418ATPSequence Analysis

    GO - Molecular functioni

    1. arsenite-transmembrane transporting ATPase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. detoxification of arsenic-containing substance Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Arsenical resistance

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Protein family/group databases

    TCDBi3.A.4.1.1. the arsenite-antimonite (arsab) efflux family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arsenical pump-driving ATPase (EC:3.6.3.16)
    Alternative name(s):
    Arsenical resistance ATPase
    Arsenite-translocating ATPase
    Arsenite-transporting ATPase
    Gene namesi
    Name:arsA
    Encoded oniPlasmid R7730 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 583583Arsenical pump-driving ATPasePRO_0000152251Add
    BLAST

    Proteomic databases

    PRIDEiP08690.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-16995N.

    Structurei

    Secondary structure

    1
    583
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 43
    Beta strandi9 – 146
    Helixi21 – 3414
    Beta strandi39 – 435
    Helixi50 – 534
    Beta strandi71 – 755
    Helixi78 – 9013
    Helixi91 – 933
    Turni94 – 963
    Helixi99 – 10810
    Helixi112 – 12817
    Helixi132 – 1354
    Beta strandi137 – 1437
    Helixi147 – 1548
    Helixi156 – 1594
    Beta strandi167 – 1693
    Helixi170 – 1723
    Helixi174 – 1785
    Helixi183 – 19412
    Turni196 – 1983
    Beta strandi199 – 2079
    Helixi209 – 22517
    Beta strandi230 – 2378
    Helixi240 – 2434
    Helixi247 – 26115
    Helixi265 – 2684
    Beta strandi272 – 2765
    Helixi285 – 2906
    Helixi315 – 3239
    Beta strandi328 – 3336
    Helixi340 – 35314
    Beta strandi358 – 3636
    Beta strandi380 – 3845
    Helixi387 – 40317
    Helixi408 – 41710
    Helixi421 – 43212
    Turni433 – 4353
    Helixi436 – 4405
    Beta strandi442 – 4476
    Helixi452 – 4609
    Helixi482 – 4865
    Turni488 – 4903
    Beta strandi491 – 4977
    Helixi501 – 51616
    Beta strandi523 – 5308
    Helixi538 – 55518
    Turni556 – 5583
    Beta strandi560 – 5667
    Helixi575 – 5828

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1F48X-ray2.30A1-583[»]
    1IHUX-ray2.15A1-583[»]
    1II0X-ray2.40A/B1-583[»]
    1II9X-ray2.60A/B1-583[»]
    ProteinModelPortaliP08690.
    SMRiP08690. Positions 1-583.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08690.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the arsA ATPase family.Curated

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR025723. Anion-transp_ATPase-like_dom.
    IPR027541. Ars_ATPase.
    IPR016300. ATPase_ArsA/GET3.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10803. PTHR10803. 1 hit.
    PfamiPF02374. ArsA_ATPase. 3 hits.
    [Graphical view]
    PIRSFiPIRSF001327. Arsenical_pump-driving_ATPase. 1 hit.
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR04291. arsen_driv_ArsA. 1 hit.
    TIGR00345. GET3_arsA_TRC40. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P08690-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQFLQNIPPY LFFTGKGGVG KTSISCATAI RLAEQGKRVL LVSTDPASNV    50
    GQVFSQTIGI TIQAIASVPG LSALEIDPQA AAQQYRARIV DPIKGVLPDD 100
    VVSSINEQLS GACTTEIAAF DEFTGLLTDA SLLTRFDHII FDTAPTGHTI 150
    RLLQLPGAWS SFIDSNPEGA SCLGPMAGLE KQREQYAYAV EALSDPKRTR 200
    LVLVARLQKS TLQEVARTHL ELAAIGLKNQ YLVINGVLPK TEAANDTLAA 250
    AIWEREQEAL ANLPADLAGL PTDTLFLQPV NMVGVSALSR LLSTQPVASP 300
    SSDEYLQQRP DIPSLSALVD DIARNEHGLI MLMGKGGVGK TTMAAAIAVR 350
    LADMGFDVHL TTSDPAAHLS MTLNGSLNNL QVSRIDPHEE TERYRQHVLE 400
    TKGKELDEAG KRLLEEDLRS PCTEEIAVFQ AFSRVIREAG KRFVVMDTAP 450
    TGHTLLLLDA TGAYHREIAK KMGEKGHFTT PMMLLQDPER TKVLLVTLPE 500
    TTPVLEAANL QADLERAGIH PWGWIINNSL SIADTRSPLL RMRAQQELPQ 550
    IESVKRQHAS RVALVPVLAS EPTGIDKLKQ LAG 583
    Length:583
    Mass (Da):63,188
    Last modified:January 1, 1988 - v1
    Checksum:i658C5AF65E5E75F5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02591 Genomic DNA. Translation: AAA21094.1.
    PIRiA25937.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02591 Genomic DNA. Translation: AAA21094.1 .
    PIRi A25937.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1F48 X-ray 2.30 A 1-583 [» ]
    1IHU X-ray 2.15 A 1-583 [» ]
    1II0 X-ray 2.40 A/B 1-583 [» ]
    1II9 X-ray 2.60 A/B 1-583 [» ]
    ProteinModelPortali P08690.
    SMRi P08690. Positions 1-583.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-16995N.

    Protein family/group databases

    TCDBi 3.A.4.1.1. the arsenite-antimonite (arsab) efflux family.

    Proteomic databases

    PRIDEi P08690.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P08690.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR025723. Anion-transp_ATPase-like_dom.
    IPR027541. Ars_ATPase.
    IPR016300. ATPase_ArsA/GET3.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10803. PTHR10803. 1 hit.
    Pfami PF02374. ArsA_ATPase. 3 hits.
    [Graphical view ]
    PIRSFi PIRSF001327. Arsenical_pump-driving_ATPase. 1 hit.
    SMARTi SM00382. AAA. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR04291. arsen_driv_ArsA. 1 hit.
    TIGR00345. GET3_arsA_TRC40. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the structural genes for an anion pump. The plasmid-encoded arsenical resistance operon."
      Chen C.-M., Misra T.K., Silver S., Rosen B.P.
      J. Biol. Chem. 261:15030-15038(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase."
      Rosen B.P.
      Res. Microbiol. 141:336-341(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiARSA1_ECOLX
    AccessioniPrimary (citable) accession number: P08690
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3