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P08690

- ARSA1_ECOLX

UniProt

P08690 - ARSA1_ECOLX

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Protein

Arsenical pump-driving ATPase

Gene
arsA
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Anion-transporting ATPase. Catalyzes the extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents.

Catalytic activityi

ATP + H2O + arsenite(In) = ADP + phosphate + arsenite(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228ATP Reviewed prediction
Nucleotide bindingi334 – 3418ATP Reviewed prediction

GO - Molecular functioni

  1. arsenite-transmembrane transporting ATPase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. detoxification of arsenic-containing substance Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arsenical resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.4.1.1. the arsenite-antimonite (arsab) efflux family.

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenical pump-driving ATPase (EC:3.6.3.16)
Alternative name(s):
Arsenical resistance ATPase
Arsenite-translocating ATPase
Arsenite-transporting ATPase
Gene namesi
Name:arsA
Encoded oniPlasmid R7730 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 583583Arsenical pump-driving ATPasePRO_0000152251Add
BLAST

Proteomic databases

PRIDEiP08690.

Interactioni

Protein-protein interaction databases

DIPiDIP-16995N.

Structurei

Secondary structure

1
583
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 43
Beta strandi9 – 146
Helixi21 – 3414
Beta strandi39 – 435
Helixi50 – 534
Beta strandi71 – 755
Helixi78 – 9013
Helixi91 – 933
Turni94 – 963
Helixi99 – 10810
Helixi112 – 12817
Helixi132 – 1354
Beta strandi137 – 1437
Helixi147 – 1548
Helixi156 – 1594
Beta strandi167 – 1693
Helixi170 – 1723
Helixi174 – 1785
Helixi183 – 19412
Turni196 – 1983
Beta strandi199 – 2079
Helixi209 – 22517
Beta strandi230 – 2378
Helixi240 – 2434
Helixi247 – 26115
Helixi265 – 2684
Beta strandi272 – 2765
Helixi285 – 2906
Helixi315 – 3239
Beta strandi328 – 3336
Helixi340 – 35314
Beta strandi358 – 3636
Beta strandi380 – 3845
Helixi387 – 40317
Helixi408 – 41710
Helixi421 – 43212
Turni433 – 4353
Helixi436 – 4405
Beta strandi442 – 4476
Helixi452 – 4609
Helixi482 – 4865
Turni488 – 4903
Beta strandi491 – 4977
Helixi501 – 51616
Beta strandi523 – 5308
Helixi538 – 55518
Turni556 – 5583
Beta strandi560 – 5667
Helixi575 – 5828

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F48X-ray2.30A1-583[»]
1IHUX-ray2.15A1-583[»]
1II0X-ray2.40A/B1-583[»]
1II9X-ray2.60A/B1-583[»]
ProteinModelPortaliP08690.
SMRiP08690. Positions 1-583.

Miscellaneous databases

EvolutionaryTraceiP08690.

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR025723. Anion-transp_ATPase-like_dom.
IPR027541. Ars_ATPase.
IPR016300. ATPase_ArsA/GET3.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10803. PTHR10803. 1 hit.
PfamiPF02374. ArsA_ATPase. 3 hits.
[Graphical view]
PIRSFiPIRSF001327. Arsenical_pump-driving_ATPase. 1 hit.
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR04291. arsen_driv_ArsA. 1 hit.
TIGR00345. GET3_arsA_TRC40. 1 hit.

Sequencei

Sequence statusi: Complete.

P08690-1 [UniParc]FASTAAdd to Basket

« Hide

MQFLQNIPPY LFFTGKGGVG KTSISCATAI RLAEQGKRVL LVSTDPASNV    50
GQVFSQTIGI TIQAIASVPG LSALEIDPQA AAQQYRARIV DPIKGVLPDD 100
VVSSINEQLS GACTTEIAAF DEFTGLLTDA SLLTRFDHII FDTAPTGHTI 150
RLLQLPGAWS SFIDSNPEGA SCLGPMAGLE KQREQYAYAV EALSDPKRTR 200
LVLVARLQKS TLQEVARTHL ELAAIGLKNQ YLVINGVLPK TEAANDTLAA 250
AIWEREQEAL ANLPADLAGL PTDTLFLQPV NMVGVSALSR LLSTQPVASP 300
SSDEYLQQRP DIPSLSALVD DIARNEHGLI MLMGKGGVGK TTMAAAIAVR 350
LADMGFDVHL TTSDPAAHLS MTLNGSLNNL QVSRIDPHEE TERYRQHVLE 400
TKGKELDEAG KRLLEEDLRS PCTEEIAVFQ AFSRVIREAG KRFVVMDTAP 450
TGHTLLLLDA TGAYHREIAK KMGEKGHFTT PMMLLQDPER TKVLLVTLPE 500
TTPVLEAANL QADLERAGIH PWGWIINNSL SIADTRSPLL RMRAQQELPQ 550
IESVKRQHAS RVALVPVLAS EPTGIDKLKQ LAG 583
Length:583
Mass (Da):63,188
Last modified:January 1, 1988 - v1
Checksum:i658C5AF65E5E75F5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02591 Genomic DNA. Translation: AAA21094.1.
PIRiA25937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02591 Genomic DNA. Translation: AAA21094.1 .
PIRi A25937.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F48 X-ray 2.30 A 1-583 [» ]
1IHU X-ray 2.15 A 1-583 [» ]
1II0 X-ray 2.40 A/B 1-583 [» ]
1II9 X-ray 2.60 A/B 1-583 [» ]
ProteinModelPortali P08690.
SMRi P08690. Positions 1-583.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-16995N.

Protein family/group databases

TCDBi 3.A.4.1.1. the arsenite-antimonite (arsab) efflux family.

Proteomic databases

PRIDEi P08690.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P08690.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR025723. Anion-transp_ATPase-like_dom.
IPR027541. Ars_ATPase.
IPR016300. ATPase_ArsA/GET3.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10803. PTHR10803. 1 hit.
Pfami PF02374. ArsA_ATPase. 3 hits.
[Graphical view ]
PIRSFi PIRSF001327. Arsenical_pump-driving_ATPase. 1 hit.
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR04291. arsen_driv_ArsA. 1 hit.
TIGR00345. GET3_arsA_TRC40. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the structural genes for an anion pump. The plasmid-encoded arsenical resistance operon."
    Chen C.-M., Misra T.K., Silver S., Rosen B.P.
    J. Biol. Chem. 261:15030-15038(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase."
    Rosen B.P.
    Res. Microbiol. 141:336-341(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiARSA1_ECOLX
AccessioniPrimary (citable) accession number: P08690
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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