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P08690 (ARSA1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Arsenical pump-driving ATPase
EC=3.6.3.16
Alternative name(s):
Arsenical resistance ATPase
Arsenite-translocating ATPase
Arsenite-transporting ATPase
Gene names
Name:arsA
Encoded onPlasmid R773
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Anion-transporting ATPase. Catalyzes the extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents.

Catalytic activity

ATP + H2O + arsenite(In) = ADP + phosphate + arsenite(Out).

Sequence similarities

Belongs to the arsA ATPase family.

Ontologies

Keywords
   Biological processArsenical resistance
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical term3D-structure
Plasmid
Gene Ontology (GO)
   Biological processdetoxification of arsenic-containing substance

Inferred from electronic annotation. Source: InterPro

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

arsenite-transmembrane transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 583583Arsenical pump-driving ATPase
PRO_0000152251

Regions

Nucleotide binding15 – 228ATP Potential
Nucleotide binding334 – 3418ATP Potential

Secondary structure

1 .......................................................................................... 583
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08690 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 658C5AF65E5E75F5

FASTA58363,188
        10         20         30         40         50         60 
MQFLQNIPPY LFFTGKGGVG KTSISCATAI RLAEQGKRVL LVSTDPASNV GQVFSQTIGI 

        70         80         90        100        110        120 
TIQAIASVPG LSALEIDPQA AAQQYRARIV DPIKGVLPDD VVSSINEQLS GACTTEIAAF 

       130        140        150        160        170        180 
DEFTGLLTDA SLLTRFDHII FDTAPTGHTI RLLQLPGAWS SFIDSNPEGA SCLGPMAGLE 

       190        200        210        220        230        240 
KQREQYAYAV EALSDPKRTR LVLVARLQKS TLQEVARTHL ELAAIGLKNQ YLVINGVLPK 

       250        260        270        280        290        300 
TEAANDTLAA AIWEREQEAL ANLPADLAGL PTDTLFLQPV NMVGVSALSR LLSTQPVASP 

       310        320        330        340        350        360 
SSDEYLQQRP DIPSLSALVD DIARNEHGLI MLMGKGGVGK TTMAAAIAVR LADMGFDVHL 

       370        380        390        400        410        420 
TTSDPAAHLS MTLNGSLNNL QVSRIDPHEE TERYRQHVLE TKGKELDEAG KRLLEEDLRS 

       430        440        450        460        470        480 
PCTEEIAVFQ AFSRVIREAG KRFVVMDTAP TGHTLLLLDA TGAYHREIAK KMGEKGHFTT 

       490        500        510        520        530        540 
PMMLLQDPER TKVLLVTLPE TTPVLEAANL QADLERAGIH PWGWIINNSL SIADTRSPLL 

       550        560        570        580 
RMRAQQELPQ IESVKRQHAS RVALVPVLAS EPTGIDKLKQ LAG

« Hide

References

[1]"Nucleotide sequence of the structural genes for an anion pump. The plasmid-encoded arsenical resistance operon."
Chen C.-M., Misra T.K., Silver S., Rosen B.P.
J. Biol. Chem. 261:15030-15038(1986) [PubMed: 3021763] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase."
Rosen B.P.
Res. Microbiol. 141:336-341(1990) [PubMed: 1704144] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J02591 Genomic DNA. Translation: AAA21094.1.
PIRA25937.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1F48X-ray2.30A1-583[»]
1IHUX-ray2.15A1-583[»]
1II0X-ray2.40A/B1-583[»]
1II9X-ray2.60A/B1-583[»]
ProteinModelPortalP08690.
SMRP08690. Positions 1-583.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-16995N.

Protein family/group databases

TCDB3.A.4.1.1. arsenite-antimonite (ArsAB) efflux family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003593. ATPase_AAA+_core.
IPR016300. ATPase_ArsA/Get3.
[Graphical view]
PANTHERPTHR10803. PTHR10803. 1 hit.
PIRSFPIRSF001327. Arsenical_pump-driving_ATPase. 1 hit.
SMARTSM00382. AAA. 2 hits.
[Graphical view]
TIGRFAMsTIGR00345. ArsA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameARSA1_ECOLX
AccessionPrimary (citable) accession number: P08690
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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